High-throughput Screening of Oligonucleotides for Identity and Purity Assessment Using the ACQUITY QDa Detector and ProMass for MassLynx

[ APPLICATION NOTEBOOK ] APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES Table of Contents Introduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4 [ HPLC & UPLC Separations ] Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8 Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11 Oligonucleotide…

[ APPLICATION NOTE ] Developing a Novel, Integrated LC-MS Workflow for High-resolution Monitoring and Characterization of Oligonucleotides Henry Shion, Robert Birdsall, and Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ Optimized RP-UPLC mobile phase Methods that…

ADDING COST EFFECTIVE MASS DETECTION FOR IMPROVED PRODUCTIVITY IN OLIGONUCLEOTIDE SCREENING ASSAYS Robert E. Birdsall, Sean M. McCarthy, Scott J. Berger, Maki Terasaki, Kenji Hiroseand Ying Qing Yu Biopharmaceutical Business Team, Waters Corporation, Milford, MA and Tokyo, Japan TUV Absorbance…

Adding Mass Detection to Synthetic Oligonucleotide Analyses with the ACQUITY QDa Detector Robert E. Birdsall and Ying Qing Yu Waters Corporation, Milford, MA, USA A P P L I C AT I O N B E N E F I…