Mobile Phase Optimization in SEC Method Development

Application Note Pharma and Biopharma SEC Method Transfer from Diol Chemistry to Polymer-Coated Silica Author Richard Hurteau Agilent Technologies, Inc. Abstract The Agilent AdvanceBio SEC 130 and 300 Å columns contain highly uniform, 2.7-µm particles, with a low-binding, polymer-coated silica…

Agilent Biocolumns Aggregate/Fragment Analysis Application Compendium Contents Background 2 Getting Started 3 How to Guide - Size Exclusion Chromatography for Biomolecule Analysis - 5991-3651EN 4 Featured Application Notes Elevate Your mAb Aggregate Analysis - 5994-2709EN 1 22 22 High-Resolution, High-Throughput…

Agilent “How to” Guide for SIZE EXCLUSION CHROMATOGRAPHY FOR BIOMOLECULE ANALYSIS A GUIDE TO SUCCESSFUL SEC The chromatographic separation of biomolecules based on their size in solution is known as size exclusion chromatography (SEC). Unlike other modes of chromatography, it…

Agilent-NISTmAb Aggregate and Fragment Analysis Agilent BioHPLC Columns Application Compendium Contents Agilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted from NISTmAb RM 8671 batch. Quality control (QC) testing is performed using Agilent LC-MS system. QC batch release test includes aggregate profile,…