Controlling the Agilent 1260 Infinity/1290 Infinity II Multisampler (G7167A/B) in Waters Empower 3 Environment

Significance of the Topic

Automated sampling with liquid chromatography modules is essential for high-throughput laboratories, reducing manual error and improving data quality. Integrating the Agilent 1260 Infinity/1290 Infinity II Multisamplers with Waters Empower 3 through the Agilent Instrument Control Framework (ICF) enables seamless instrument management in third-party chromatography data systems.

Objectives and Study Overview

This technical note provides step-by-step instructions for configuring and controlling the Agilent Multisampler in Waters Empower 3. It covers prerequisites, well plate definition, method setup, and sample analysis workflows.

Methodology and Instrumentation Used

• Agilent ICF and Waters Instrument Control Software to bridge communication between Empower 3 and Agilent LC hardware
• Required firmware and software: Multisampler firmware Rev. D.06.60 or higher; Waters ICS 2.1 Hotfix with Agilent ICF A.02.03 DU1 HF2 and ICF-LC Driver A.02.11 SP1
• Empower system configuration via DHCP-based instrument detection, module scanning, and chromatographic system creation
• Well plate import or manual definition in Empower, assignment to Multisampler drawers, and capillary configuration
• Instrument method setup in Empower including injection parameters, cleaning routines, and priming

Main Results and Discussion

The guide confirms reliable control of the Multisampler through Empower 3, ensuring correct mapping of plate types and system modules. It enables both single and series injections, with optional cleaning protocols to minimize carryover and maintain performance.

Benefits and Practical Applications of the Method

• Faster method development using standardized ICF drivers
• Improved reproducibility for pharmaceutical, environmental, and biochemical screening
• Flexible plate and cleaning configurations support diverse sample needs
• Third-party CDS compatibility expands integration across laboratory environments

Future Trends and Potential Applications

Emerging developments may include cloud-based CDS integration, AI-driven sampling optimization, support for new microplate formats, and advanced self-diagnostics to further reduce downtime in automated workflows.

Conclusion

Utilizing Agilent Multisamplers with Waters Empower via ICF offers a robust solution for automated sample handling, enhancing laboratory efficiency, data integrity, and throughput. The documented configuration and cleaning procedures support modern analytical demands.

References

No external literature references were provided in the original document.