The Agilent AdvanceBio SEC 300Å HPLC column (4.6 × 300 mm, 2.7 µm) is specifically designed for size exclusion chromatography (SEC) of proteins and biomolecules. Engineered with fully porous 2.7 µm particles and a 300 Å pore size, this column provides high-resolution separation and reproducibility for aggregate and fragment analysis in biopharmaceutical workflows. It is particularly well-suited for characterizing monoclonal antibodies (mAbs) and protein aggregates across a wide molecular weight range of 5–1,250 kDa.
Constructed with durable stainless-steel hardware, the column supports pressures up to 400 bar and withstands operating temperatures up to 80 °C. Its broad pH tolerance (2–8.5) ensures versatility for diverse applications. The column delivers consistent, reliable performance for advanced protein analysis in biopharma laboratories.
Key Features
- Optimized for protein and mAb aggregate/fragment analysis
- Fully porous 2.7 µm particles with 300 Å pore size for high-resolution SEC
- Broad molecular weight separation range: 5–1,250 kDa
- Stainless-steel hardware rated to 400 bar and 80 °C
- Suitable for LC and LC/MS biopharma workflows
Technical Specifications
- Brand: AdvanceBio SEC
- Hardware: Stainless Steel (SS)
- Inner Diameter (ID): 4.6 mm
- Length: 300 mm
- Particle Size: 2.7 µm
- Particle Type: Fully Porous
- Phase: SEC
- Pore Size: 300 Å
- Pressure Rating: 400 bar
- Maximum Temperature: 80 °C
- Molecular Weight Range: 5,000 – 1,250,000 Da
- pH Range: 2.0 – 8.5
- Separation Mode: Size Exclusion Chromatography (SEC)
- Application Domain: Biopharma
- Typical Applications:
- Protein aggregate and fragment analysis
- Monoclonal antibodies (mAbs)
- Proteins
- LC Platform: Stainless Steel
- Technique: LC
- Shipping Solvent: 0.02% Sodium Azide
- UNSPSC Code: 41115711
