The Agilent 1260 Infinity HPLC + SCIEX Triple Quad 5500 is a complete LC-MS/MS platform designed for highly sensitive and selective quantitative analysis of organic compounds in complex matrices. The offered configuration combines a SCIEX Triple Quad 5500 triple-quadrupole mass spectrometer with ESI and an Agilent 1260 Infinity HPLC equipped with a binary pump, autosampler, and thermostatted column compartment.
The SCIEX Triple Quad 5500 is primarily optimized for robust MRM quantitation, combining fast eQ™ Electronics with precursor/product-ion selection and the Turbo V™ ion source. The Agilent 1260 Infinity Binary LC provides high-pressure binary gradients up to 600 bar, flow rates up to 5 mL/min, and low delay volume for fast HPLC/UHPLC and LC-MS separations.
Offered Configuration
- SCIEX Triple Quad 5500 LC-MS/MS
- ESI – electrospray ionization
- Turbo V™ ion source
- Triple-quadrupole mass analyzer
- Roughing / vacuum pump
- Agilent 1260 Infinity HPLC
- Binary Pump
- Autosampler
- Thermostatted Column Compartment
SCIEX Triple Quad 5500
Triple-Quadrupole Architecture
The system uses:
- Q1 for precursor-ion isolation,
- q2 for collision-induced dissociation,
- Q3 for product-ion selection.
This architecture provides the high selectivity required for Multiple Reaction Monitoring in complex samples.
Turbo V™ ESI Source
The offered configuration uses electrospray ionization with the Turbo V source.
Its orthogonal spray architecture is designed for robust desolvation and stable performance across demanding LC-MS/MS workflows. SCIEX positions the original Triple Quad 5500 specifically for high sensitivity and robustness in complex matrices.
Acquisition Modes
Supported triple-quadrupole workflows include:
- MRM
- Q1 Scan
- Product Ion Scan
- Precursor Ion Scan
- Neutral Loss Scan
MRM is the principal mode for sensitive quantitative analysis.
Agilent 1260 Infinity Binary LC
The 1260 Infinity Binary LC combines high-resolution LC performance with backwards compatibility for conventional HPLC methods.
Binary Pump
- Gradient formation: high-pressure binary
- Maximum pressure: 600 bar
- Operating flow range: approximately 0.05–5.0 mL/min
- Configurable delay volume down to approximately 120 µL
- Optimized for HPLC, UHPLC, and LC-MS applications.
Autosampler
For the standard high-performance 1260 Infinity configuration:
- Injection range: 0.1–100 µL
- High injection precision
- Low carryover
- Vial and microplate support
- Operation at system pressures up to 600 bar.
Thermostatted Column Compartment
Depending on the exact installed module, the TCC provides:
- active heating and cooling,
- cooling to approximately 10 °C below ambient,
- upper temperature limit of 80–100 °C depending on model,
- support for multiple analytical columns.
LC-MS/MS Workflow
The combined platform follows the analytical path:
autosampler → binary gradient → thermostatted column → ESI → Q1 → q2 → Q3 → detector
The LC provides reproducible chromatographic separation while the triple quadrupole provides sensitive and selective targeted quantitation.
Main Technical Specifications
SCIEX Triple Quad 5500
- Analyzer: Triple quadrupole
- Ionization in Offered Configuration: ESI
- Ion Source: Turbo V™
- Polarity: Positive / negative
- Primary Acquisition: MRM
- Additional Modes: Q1, Product Ion, Precursor Ion, Neutral Loss
- Software: Analyst®, depending on supplied license
- Vacuum System: Included with MS configuration
- Intended Use: Research Use Only unless the specific installed system is otherwise certified.
Agilent 1260 Infinity HPLC
- Technique: HPLC / UHPLC
- Gradient Formation: High-pressure binary
- Maximum Pressure: 600 bar
- Pump Flow Range: 0.05–5.0 mL/min
- Typical Injection Range: 0.1–100 µL
- Autosampler: Automated
- Column Thermostat: Included
- Temperature Range: Depends on exact installed TCC
- Function: Chromatographic separation prior to LC-MS/MS.
Typical Applications
- Pharmaceutical quantitative analysis
- Bioanalysis
- Pharmacokinetics
- Drug metabolites
- Research therapeutic drug monitoring
- Forensic toxicology
- Pesticides
- Veterinary drugs
- Mycotoxins
- PFAS
- Environmental contaminants
- Food analysis
- Hormones and steroids
- Lipid mediators
- Targeted metabolomics
- Peptide quantitation
