The Agilent 6460C LC/QQQ is a sensitive triple quadrupole tandem mass spectrometer designed primarily for targeted quantitative LC/MS/MS analysis. The offered configuration combines the 6460C with an Agilent 1290 Infinity II UHPLC system consisting of a G7120A High Speed Pump, G7167B Multisampler, and G7116B Multicolumn Thermostat. Together, these modules provide rapid gradient separations, operation up to 1300 bar, automated high-capacity sample handling, and precise column temperature control in a fully integrated LC/QQQ workflow.
Offered Configuration
- G6460C: Agilent 6460 Triple Quadrupole LC/MS
- G7120A: Agilent 1290 Infinity II High Speed Pump
- G7167B: Agilent 1290 Infinity II Multisampler
- G7116B: Agilent 1290 Infinity II Multicolumn Thermostat
Agilent 6460C Triple Quadrupole LC/MS
- Mass Range: m/z 5–3000
- Maximum Scan Rate: 12,500 Da/s
- Polarity Switching: 30 ms
- Autotune Resolution: 0.7 Da FWHM
- Manual Resolution: 0.5 Da FWHM
- Mass Accuracy:
- 0.1 Da from m/z 5–1000
- 0.01% from m/z 1000–2000
- 0.02% from m/z 2000–3000
- Mass Stability: <0.1 Da over 24 h
- Detector Dynamic Range: >6 × 10⁶
- Minimum MRM Dwell Time: 1 ms
- MRM Transitions: up to 450 per time segment
- Dynamic MRM: up to 4000 transitions per method
- Triggered MRM: up to 10 transitions per compound
- Collision Cell Clearance: <1 ms
Supported acquisition modes include:
- MS Scan
- Product Ion Scan
- MRM
- Dynamic MRM
- Triggered MRM
- Precursor Ion Scan
- Neutral Loss/Gain Scan
- SIM
G7120A – 1290 Infinity II High Speed Pump
- Binary high-pressure gradient pump
- Settable Flow Range: 0.001–5.0 mL/min
- Recommended Flow Range: 0.05–5.0 mL/min
- Maximum Pressure: 1300 bar
- Flow Precision: ≤0.07% RSD or 0.005 min SD
- Pump Resolution: 300 pL
- Delay Volume: down to approximately 10 µL
- Integrated degasser
- Active seal wash
- Jet Weaver mixer
- Active pressure damping
- ISET method-emulation capability
G7167B – 1290 Infinity II Multisampler
- Maximum Pressure: 1300 bar
- Standard Injection Range: 0.1–20 µL
- Optional Injection Range: 40 or 100 µL
- Multi-Draw: up to 1500 µL depending on configuration
- Injection Precision: <0.15% RSD or SD <10 nL
- Injection Linearity: 0.9999 from 0.1–100 µL
- Sample Viscosity: 0.2–5.0 cP
- Maximum Capacity:
- up to 6144 samples in 384-well format
- up to 1536 samples in 96-well format
- Vials, Eppendorf tubes, and deep-well plates supported
- Internal and external needle wash
- Needle-seat backflush capability
- Optimized for very low carryover
G7116B – 1290 Infinity II Multicolumn Thermostat
- Two independent Peltier-controlled zones
- Temperature Range:
- up to 20 °C below ambient
- minimum 4 °C
- maximum 110 °C
- Temperature Setting Resolution: 0.1 °C
- Temperature Stability: ±0.03 °C
- Temperature Accuracy: ±0.5 °C after calibration at 40 °C
- Temperature Precision: 0.05 °C
- Column Capacity:
- up to 8 × 100 mm columns
- or 4 × 300 mm columns
- Optional automated eight-column selection
- High-pressure valve options up to 1300 bar
Typical Applications
- Targeted quantitative LC/MS/MS
- Pharmaceutical analysis
- Bioanalysis and DMPK
- Drug and metabolite quantitation
- Forensic toxicology
- Clinical research
- Pesticide and residue analysis
- Mycotoxins
- Veterinary drugs
- PFAS and environmental contaminants
- Food analysis
- Trace analysis in complex matrices
- Multi-residue screening
- Dynamic MRM workflows
