The Agilent 6230B LC/TOF is a high-resolution accurate-mass LC/MS system based on time-of-flight mass spectrometry. The offered configuration combines the Agilent 6230B mass spectrometer with a modular Agilent 1200 Series HPLC consisting of the G1311A Quaternary Pump, G1329A Standard Autosampler with G1330B Thermostat, G1316A Thermostatted Column Compartment, and G1315D Diode Array Detector. A Peak Scientific Genius NM32LA nitrogen generator with an integrated compressor is also included.
The combination of LC separation, diode-array detection, and accurate-mass TOF detection provides both UV/Vis chromatographic information and high-resolution mass spectral data. The system is suitable for screening, unknown identification, molecular formula confirmation, complex sample profiling, and quantitative LC/TOF workflows.
Offered System Configuration
- Agilent 6230B: Time-of-Flight LC/MS with G3251B - Dual Sprayer ESI Source
- G1311A: Agilent 1200 Series Quaternary Pump
- G1329A: Agilent 1200 Series Standard Autosampler
- G1330B: Thermostat for 1200 ALS/Fraction Collector
- G1316A: Agilent 1200 Series Thermostatted Column Compartment
- G1315D: Agilent Diode Array Detector
- Peak Scientific Genius NM32LA: LC/MS Nitrogen Generator
Agilent 6230B TOF LC/MS
- Mass resolution: >10,000 at m/z 118
- Mass resolution: >22,000 at m/z 1522
- Mass accuracy: <1 ppm for calibration masses in high-resolution mode
For a Dual AJS configuration, installation verification specifies >50:1 S/N for 1 pg reserpine and <1 ppm mass accuracy using 20 pg reserpine. For Dual ESI, the specified S/N is >10:1 for 1 pg reserpine. Ion source is G3251B - Dual Sprayer ESI Source.
G1311A – Agilent 1200 Series Quaternary Pump
- Flow range: 0.2–10.0 mL/min
- Settable range: 0.001–10 mL/min
- Pressure:
- 400 bar up to 5 mL/min
- 200 bar up to 10 mL/min
- Flow accuracy: ±1% or 10 µL/min
- Flow precision: ≤0.07% RSD
- Low-pressure quaternary gradient mixing
- Recommended pH range: 1.0–12.5
- Gradient delay volume: approximately 800–1100 µL
- Solvent compressibility compensation
- Early Maintenance Feedback
G1329A – Agilent 1200 Series Standard Autosampler
- Injection range: 0.1–100 µL
- Multi-Draw capability: up to 1500 µL
- Maximum pressure: 400 bar
- Capacity: 100 × 2 mL vials
- Replicate injections: 1–99 per vial
- Precision:
- <0.25% RSD from 5–100 µL
- <1% RSD from 1–5 µL
- Typical carryover: <0.1%
- Typical carryover with external needle wash: <0.05%
- Sample viscosity range: 0.2–50 cP
G1330B – Autosampler Thermostat
- Sample temperature setting: 4–40 °C
- 1 °C increments
- Cooling capability: approximately 10 °C below ambient
- Dimensions: 140 × 345 × 435 mm
- Weight: 18.5 kg
- Maximum active power: 210 W
G1316A – Thermostatted Column Compartment
- Temperature range: 10 °C below ambient to 80 °C
- Temperature stability: ±0.15 °C
- Temperature accuracy: ±0.8 °C
- Calibrated accuracy: ±0.5 °C
- Capacity: up to three 300 mm columns
- Heat-up from ambient to 40 °C: approximately 5 min
- Cool-down from 40 to 20 °C: approximately 10 min
G1315D – Diode Array Detector
- Detection: 1024-element photodiode array
- Light sources:
- deuterium lamp
- tungsten lamp
- Wavelength range: 190–950 nm
- Data acquisition: up to 20 Hz
- Wavelength accuracy: ±1 nm
- Programmable slit width: 1, 2, 4, 8, or 16 nm
- Linear absorbance range: >2 AU
Peak Scientific Genius NM32LA Nitrogen Generator
The included NM32LA provides an on-demand nitrogen supply for LC/MS applications and incorporates its own compressor.
- Gas: Nitrogen
- Maximum flow: 32 L/min
- Maximum outlet pressure: 100 psi / 6.9 bar
- Outlet fitting: 1/4" BSP
- Integrated compressor: Yes
- Startup time: 30 min
- Particle specification: <0.01 µm
- Power consumption: 1680 VA
- Supply: 110 / 240 VAC ±10%
- Frequency: 50/60 Hz
- Maximum operating temperature: 30 °C
- Dimensions: 713 × 600 × 750 mm
- Weight: 103 kg
- Certifications: CE, CSA
