This Agilent 1260 Infinity configuration is designed for analytical workflows requiring not only flexible quaternary gradients but also controlled sample temperature, reproducible column conditions, and comprehensive UV/Vis detection. The G1329B Standard Autosampler combined with the G1330B Thermostat maintains samples under controlled conditions throughout a sequence, while the G1316A stabilizes column temperature. The G4212B DAD then provides up to eight simultaneous chromatographic signals together with full UV/Vis spectral acquisition at rates up to 80 Hz.
System Components
G1311B – 1260 Infinity Quaternary Pump
- Flow range: 0.2–10.0 mL/min
- Settable flow: 0.001–10 mL/min
- Pressure:
- up to 600 bar at ≤5 mL/min
- up to 200 bar above 5 to 10 mL/min
- Flow accuracy: ±1% or 10 µL/min
- Flow precision: ≤0.07% RSD
- Low-pressure quaternary gradient formation
- Up to four solvents
- Delay volume: approximately 600–800 µL
- Recommended pH range: 1.0–12.5
- Solvent compressibility compensation
- Early Maintenance Feedback
G1329B – 1260 Infinity Standard Autosampler
- Injection volume: 0.1–100 µL
- Up to 1500 µL with Multi-Draw configuration
- Maximum pressure: 600 bar
- Capacity: 100 × 2 mL vials
- Replicate injections: 1–99
- Typical precision:
- <0.25% RSD above 5 µL
- <1% RSD from 1–5 µL
- Carryover: typically <0.1%
- With external needle wash: typically <0.05%
- Sample viscosity: 0.2–50 cP
G1330B – Autosampler Thermostat
- Temperature setting: 4–40 °C
- Cooling capability: approximately 10 °C below ambient
- Dimensions: 140 × 345 × 435 mm
- Power: 100–240 VAC
- Maximum active power: approximately 210 W
G1316A – Thermostatted Column Compartment
- Temperature range: 10 °C below ambient to 80 °C
- Stability: ±0.15 °C
- Accuracy: ±0.8 °C, or ±0.5 °C after calibration
- Capacity: up to three 300 mm columns
- Heat-up to 40 °C: approximately 5 min
- Cool-down 40 → 20 °C: approximately 10 min
- Column identification support
G4212B – 1260 Infinity Diode Array Detector
- Detector: 1024-element diode array
- Light source: Deuterium lamp
- Wavelength range: 190–640 nm
- Wavelength accuracy: ±1 nm
- Simultaneous signals: 8
- Maximum sampling rate: 80 Hz
- Slit width: 4 nm
- Spectral libraries and peak-purity analysis
- Standard Max-Light cartridge cell:
- 10 mm path length
- approximately 1 µL σ dispersion volume
Typical Applications
- Routine HPLC analysis
- Pharmaceutical quality control
- Food and beverage analysis
- Environmental testing
- Chemical analysis
- Method development and validation
- UV/Vis spectral identification
- Peak-purity assessment
- Automated unattended sample sequences
