The Waters ACQUITY UPLC 30 cm Column Heater/Cooler (30-cm CHC) is a dedicated column temperature-control module for ACQUITY UPLC systems. It provides both active heating and cooling and is designed to accommodate LC columns up to 300 mm in length and 7.8 mm internal diameter. Using a thermoelectric heating/cooling engine with forced-air convection, the module maintains stable column temperatures from as much as 15 °C below ambient up to 65 °C, supporting reproducible chromatographic separations in temperature-sensitive methods.
Key Features
- Active heating and cooling of LC columns
- Temperature setpoint range of 4.0 to 65.0 °C
- Setpoint resolution of 0.1 °C
- Controllable range from up to 15 °C below ambient to 65 °C
- Supports columns up to 300 mm long and 7.8 mm ID
- Accommodates up to 6 serially connected columns
- Internal thermistor for temperature feedback
- Thermoelectric heating/cooling with forced-air convection
- Integrates with ACQUITY UPLC H-Class, H-Class Bio, and I-Class systems
- Can serve as an alternate or auxiliary column compartment depending on system configuration
- Controlled through the system console or chromatography method
- Compatible with workflows using Empower, MassLynx, and UNIFI software
Compatibility
As an alternate 30 cm column heating/cooling module:
- ACQUITY UPLC H-Class Series with CH-A
- ACQUITY UPLC H-Class Bio Series with CH-A
- ACQUITY UPLC I-Class Series with CH-A
As an auxiliary 30 cm column heating/cooling module:
- ACQUITY UPLC H-Class Series with CM-A
- ACQUITY UPLC H-Class Bio Series with CM-A
When connected to a CM-A, the 30-cm CHC can be plumbed directly to the Column Manager switching valves and used as an additional heated/cooled column zone. Operation is subject to the configuration restrictions described by Waters.
Technical Specifications
Temperature Control
- Setpoint range: 4.0–65.0 °C
- Setpoint resolution: 0.1 °C
- Controllable range: up to 15 °C below ambient to 65 °C
- Heating/cooling principle: thermoelectric engine with forced-air convection
- Feedback sensor: internal compartment thermistor
Column Capacity
- Number of columns: up to 6, serially connected
- Maximum column length: 300 mm
- Maximum column ID: 7.8 mm
- Maximum guard column or in-line filter length: 20 mm
- Adjustable rail-mounted clips for different column sizes
Dimensions and Weight
- Height: 55.2 cm
- Width: 15.2 cm
- Depth: 35.6 cm excluding drip-tray fitting
- Weight: 12.7 kg
Environmental Specifications
- Ambient operating temperature: 4–40 °C
- Relative humidity: 20–80%, noncondensing
- Acoustic noise: <58 dBA
- Rear clearance requirement: 15 cm
- Shipping/storage temperature: −30 to 60 °C
- Shipping/storage humidity: 20–85%, noncondensing
Electrical Specifications
- Power requirements: 100–240 VAC
- Line frequency: 50–60 Hz
- Power consumption: 240 VA
- Protection class: Class I
- Overvoltage category: II
- Pollution degree: 2
- Moisture protection: IPX0
Communication and Control
- 25-pin D-series sub-connector
- Control and communication via the Sample Manager
- Temperature control through the instrument console or chromatography method
Wetted Materials
- System tubing and fittings: 316 SST, PEEK, MP35N (Bio)
- Column outlet tubing and fittings: PEEK
- Drip tray: polyethylene
- Drain tubing: Tygon 2475
Typical Applications
- UPLC and HPLC separations using long columns
- Temperature-controlled LC methods
- Bio-compatible LC workflows
- Pharmaceutical analysis
- Quality control
- Research and development
- Multi-column serial configurations
- Applications requiring sub-ambient column cooling
