The Thermo Scientific Orbitrap Elite Hybrid Mass Spectrometer with ETD is a high-performance hybrid MS system combining a high-field Orbitrap™ mass analyzer with the Velos Pro dual-pressure linear ion trap, delivering exceptional resolution, sensitivity, and versatility for advanced research applications.
Key features
- High-field Orbitrap analyzer for ultra-high resolution and mass accuracy
- Resolving power >240,000 FWHM at m/z 400
- Parallel MS and MSⁿ acquisition for increased analytical throughput
- Multiple fragmentation techniques: CID, HCD, and ETD (Electron Transfer Dissociation)
- Velos Pro ion trap (Generation II) with neutral blocking technology for improved robustness
- Advanced signal processing and low-noise detection electronics
Ion sources and options
- H-ESI II heated electrospray source (required), flow rates from 1 µL/min to <2,000 µL/min
- NanoSpray Flex ion source for static and dynamic nanospray (50 nL/min–2 µL/min)
- APCI/APPI compatibility for flow rates from 50 µL/min to 2 mL/min
- ETD reagent ion source with dedicated nitrogen supply
Performance characteristics
- Mass range: m/z 50–2,000 (standard), up to m/z 4,000
- Resolution:
- 60,000 FWHM at m/z 400 (4 Hz scan rate)
- Maximum >240,000 FWHM at m/z 400
- Mass accuracy:
- <3 ppm RMS (external calibration)
- <1 ppm RMS (internal calibration)
- Dynamic range: >5,000 within a single scan
- MSⁿ capability: up to MS¹⁰
- ETD performance: >15% fragmentation efficiency (Angiotensin I)
Vacuum and hardware design
- Differentially pumped vacuum system with split-flow turbomolecular pumps
- Final operating pressure <2 × 10⁻¹⁰ Torr
- Gas-filled C-Trap, axial-field HCD collision cell
- Active temperature control of the Orbitrap analyzer (Peltier element)
Software and data system
- Thermo Scientific Xcalibur instrument control and data processing
- Data-dependent acquisition with intelligent decision tree logic
- Compatible with Proteome Discoverer, ProSightPC, SIEVE, MetWorks, Mass Frontier, and others
Typical applications
- Proteomics and PTM analysis
- Metabolomics and lipidomics
- Top-down and bottom-up protein characterization
- High-resolution qualitative and quantitative workflows
A fault was detected - insufficient rotation of tmp in the etd part of the system (8%) and an error in the embedded PC in the FT part, probably a defect in the power supply and mainboard.
