Course: Introduction to Biochromatography

Phenomenex Training Seminars 2024: Introduction to Biochromatography

Course Summary

In this basic seminar we provide a comprehensive overview of typical workflows and methods for the characterisation of proteins and peptides. We give valuable tips for sample preparation and discuss the most important optimisation parameters for chromatographic separations using size exclusion, ion exchange, reversed phase or HILIC chromatography.

Registration

Date and Location

5 November 2024

• 08:30 - 16:30 GMT | 09:30 - 17:30 CET
• Online

Price (per person)

£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)

Course Outline

• Basics about Peptides, Proteins and Antibodies
• Aggregate and fragment analysis of mAbs and other biologics using size exclusion chromatography
• Characterisation of antibody charge variants by ion exchange chromatography and optimisation of salt and pH gradients
• Use of widepore core-shell particles for the RP analysis of intact biologics and their subunits
• Improvement of resolution and peak shape in peptide mapping and peptide quantification
• Enzymatic digestion and reduction of proteins
• Sample preparation using magnetic beads
• Separation of released and labelled glycans using HILIC chromatography

Practical Skills Acquired

With the background knowledge gained, you should be able to develop and optimise a method for biologics faster and in a more targeted manner in the future by:

• Minimizing undesirable secondary interactions, problematic carryover and recovery problems through the use of biocompatible hardware components.
• Expanding the experimental design space for different separation techniques and optimising of key method parameters for both UV and MS detection.
• Using the latest column technologies to adjust selectivity according to your separation goal.