Course: How to Develop HPLC Methods

Phenomenex Training Seminars 2024: How to Develop HPLC Methods

Course Summary

Learn how to select appropriate method conditions and perform suitable investigative experiments to obtain a set of method parameters which enables the desired separation for mixtures of analytes.

This course is ideal for those who have experience running HPLC methods and now want to learn how to develop new methods.

Registration

Date and Location

28 May 2024

• 08:30 - 16:00 BST | 09:30 - 17:00 CEST
• Online

14 October 2024

• 08:30 - 16:00 BST | 09:30 - 17:00 CEST
• Online

Price (per person)

£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)

Course Outline

Developing an HPLC method using a 5-step strategy:

• Setting suitable objectives for method development
• Assessing all available information
• Selecting suitable samples
• Performing scouting experiments to select suitable initial conditions
• Optimising the method to define method parameters which achieve the desired separation

Practical Skills Acquired

This course will enable you to take a strategic approach to developing HPLC methods with an understanding of the factors which can be adjusted to manipulate the retention time of analytes.

• Define the objectives for the development of an HPLC analytical method.
• Effectively assess all the available relevant information relating to the desired method, e.g. pKa of the analyte.
• Select and prepare a suitable sample or samples to be used for the method development.
• Select suitable scouting conditions to find a suitable column and mobile phase system.
• Optimise the chromatographic conditions to result in the best possible separation.