An Introduction to LC-MS

Phenomenex Training Courses 2026: An Introduction to LC-MS

Practical Skills Acquired

Attendees will learn the basics of LC-MS allowing them to select suitable columns and mobile phases for screening. They will learn about quadrupoles and time of flight mass analysers together with how to understand the data output.

👉Registration

Price (per person)

• £445 | 510 € | 510 CHF | 1200 PLN | 3780 DKK | 5355 NOK | 5620 SEK | 9300 CZK 
• +VAT, incl. PDF of the slides and certificate of attendance

Please note: the seminars take place in two half days (2 sessions of 4 hours each).

Course Outline

Part I. Introduction to Mass Spectrometry:

This module will introduce and develop new practitioners by delivering an understanding of basic mechanisms associated with LC-MS. As well as discussing mass spectrometry fundamentals, the material will then go on to examine the most commonly applied LC-MS techniques and the principles behind them:

• Atmospheric pressure ionisation theory
  • Desolvation
  • Electrospray Ionisation (ESI)
  • Atmospheric Chemical Ionisation (APCI)
     
• Mass spectrometry definition
  • Overview of the key definitions in mass spectrometry; mass accuracy and resolution, nominal versus accurate mass and isotopic pattern identification
     
• Mass analyser: benefits, limitations and general usage
  • Quadrupole
  • Ion trap
  • Time of flight
  • Orbitrap
• Mass spectrometry workflow
  • Acquisition procedures
  • Qualitative and quantitative approach
  • Case studies

Part II. Liquid Chromatography for Mass Spectrometry:

• What is the role of the LC column in LC-MS?
• Why is it even necessary?
• Basics of LC theory
• Review of the different LC media/support particles
• Mobile phases and buffer choice for LC-MS
• Common contaminants with MS