HPLC columns are a critical component of chromatographic systems for the effective separation and analysis of components across a wide range of applications. Each HPLC column has a limited lifespan, and to maximize its utility, proper care is essential, which can extend its usage time. It is recommended to regularly monitor the column's efficiency and separation capabilities using standards. If you plan to store the column for an extended period, use a mobile phase that inhibits microbial growth (such as mobile phases containing sodium azide or higher concentrations of methanol, acetonitrile, etc.).To achieve optimal HPLC column performance and longevity, follow these recommendations:<ul><li>#1 Check the column‘s efficiency on a regular basis.</li><li>#2 Always sure that both mobile phases are well miscible when changing them.</li><li>#3 Avoid salts from precipitation in the column, e.g. high content of the organic component in the buffer.</li><li>#4 Use only perfectly filtered and degassed mobile phases.</li><li>#5 Keep the column from drying out. Close securely when not in use.</li><li>#6 For long-term preservation, use a mobile phase to inhibit the growth of mold and bacteria, for example, by adding NaN3.</li><li>#7 Use the column according in direction on the label. Reverse flow of 5 - 10 column volumes can assist in clearing a clogged inlet frit when there is a significant column backpressure.</li><li>#8 Use guard column to protect your analytical column from particulates, solvent impurities and retained sample components.</li></ul>The right column choice is as essential for successful analysis as is its subsequent care. Watrex Praha offers a broad portfolio of HPLC columns, which they prepare in-house at their laboratory.<h3>Types filling of and Applications for HPLC Columns</h3><h4>1. Reversed Phase</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=deltasil+100+c18&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex reversed-phase columns</a> use high-quality silica-based stationary phases modified with C18 alkyl chains, with effective endcapping to deactivate residual silanol groups.They are ideal for the separation of slightly polar to nonpolar compounds across a wide range of products, including components of medicines, food products and supplements, environmental pollutants or other natural products.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Figure_1_Chromatogram_1_Column_Watrex_Ion_Plus_TM_CS_19_200x4mm_36d35a09a4.jpg" alt="Watrex: Figure 1: Chromatogram 1 - Column: Watrex IonPlus CS19, 200x4mm; Mobile phase: 4mM HNO3; Flowrate: 1.0 ml/min; Detection: Conductivity without suppression. Chromatogram 2 - Column: Watrex IC Anion I; Mobile phase: 4mM Na 2 CO 3 + 1mM NaHCO 3 + 0.1mM NaSCN; Flowrate: 1,2 ml/min; Pressure: 54 bar; Detection: Conductivity with suppression; Injection: 20 μl." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Figure_1_Chromatogram_1_Column_Watrex_Ion_Plus_TM_CS_19_200x4mm_36d35a09a4.jpg 245w," sizes="100vw" width="2419" height="1181"><ul><li>Column Dimensions: Depending on customer requirements – from standard analytical columns (2.1 - 4.6 mm diameter, typical lenght 100 mm to 250 mm) packed with strictly monodisperse particles sized 3 or 5 μm. Preparative columns suitable for the separation and purification of biomolecules (peptides, amino acids, nucleotides, etc.) with larger porosity are available in semi-preparative (8 mm diameter) and preparative (20 mm diameter) formats.</li><li>Example Applications: Analysis of pharmaceuticals, environmental analyses, food composition.</li></ul><h4>2. Ion-Exchange Columns</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=ionplus&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex ion-exchange columns</a> are designed for the separation of ionic compounds, based on interactions between ions in the stationary phase and analytes. These columns are suitable for the separation of inorganic ions, amino acids, or proteins.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Figure_2_Chromatogram_3_Column_Watrex_Deltasil_100_C18_258df30fcd.jpg" alt="Watrex: Figure 2: Chromatogram 3 - Column: Watrex Deltasil 100 C18; Mobile phase: 85% ACN; Flowrate:0.65 ml/min; Pressure: 54 bar; Detection: 254 nm; Injection: 2 μl." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Figure_2_Chromatogram_3_Column_Watrex_Deltasil_100_C18_258df30fcd.jpg 245w," sizes="100vw" width="2419" height="1181"><ul><li>Column Dimensions: Columns available in various dimensions.</li><li>Example Applications: Analysis of ions, biomolecules, peptide separation.</li></ul><h4>3. Ion-Exclusion Columns</h4>These <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=polymer+iex&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex Polymer IEX HPLC columns</a> are suitable for the separation of weak organic acids and carbohydrates, based on ion exclusion principles. The basis is polymer monodisperse particles with a size of 8 μm modified by sulfonation and supplied in various ionic forms.<ul><li>The H-form is intended for the determination of weak organic acids in food or agricultural and veterinary products.</li><li>The Ca- and Pb-forms are typically used for the determination of mono-, di- and oligosaccharides in the food industry.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Figure_3_Chromatogram_4_Column_Watrex_Polymer_IEX_Ca_250x8mm_Chromatogram_5_Column_Watrex_Polymer_IEX_H_c3818e4cc1.jpg" alt="Watrex: Figure 3: Chromatogram 4 - Column: Watrex Polymer IEX Ca 250x8mm; Eluent: H2O; Flow-rate: 0.5 ml/min; Pressure: 26 bar; Inj. Volume: 20 μl; Concentration: 20 ppm; Temperature: 90 °C; Detection: RI. Chromatogram 5 - Column: Watrex Polymer IEX H 250x8mm; Eluent: 9mM H2SO4; Flow-rate: 0.5 ml/min; Pressure: 45 bar; Inj. Volume: 20 μl; Concentration: 20 ppm; Temperature: ambient; Detection: UV 210 nm." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Figure_3_Chromatogram_4_Column_Watrex_Polymer_IEX_Ca_250x8mm_Chromatogram_5_Column_Watrex_Polymer_IEX_H_c3818e4cc1.jpg 245w," sizes="100vw" width="2419" height="1181"><ul><li>Column Dimensions: 8 mm diameter and lengths of 250 or 300 mm.</li><li>Example Applications: Analysis of organic acids in food products, fermentation processes.</li></ul><h4> 4. Gel Permeation/Size Exclusion Chromatography (GPC/SEC) Columns</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=deltagel&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex gel permeation chromatography (GPC) columns</a> are used for separating highmolecular-weight compounds, typically synthetic or water-soluble polymers, based on molecule size. Smaller molecules penetrate more deeply into the porous structure of the sorbents and thus elute later than larger molecules.According to the type of stationary phase, the columns are usually divided into Gel Permeation Chromatography (GPC) columns used in the separation of synthetic polymers with organic mobile phases (e.g. tetrahydrofuran, THF, DMF, toluene THF) on polystyrene - divinylbenzene sorbents with defined porosity.In contrast, the term Size-Exclusion Chromatography (SEC) typically refers to columns for the separation of water-soluble polymers, biomacromolecules (e.g. peptides or proteins), which occur in buffered aqueous phases on porous hydrophilic sorbents (e.g. based on agarose or methacrylate particles modified with hydrophilic groups).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Figure_4_Chromatogram_6_Delta_Gel_TM_Mixed_C_Chromatogram_7_Delta_Gel_TM_Mixed_D_541ef2ed25.jpg" alt="Watrex: Figure 4: Chromatogram 6 - DeltaGelTM Mixed C. Chromatogram 7 - DeltaGelTM Mixed D." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Figure_4_Chromatogram_6_Delta_Gel_TM_Mixed_C_Chromatogram_7_Delta_Gel_TM_Mixed_D_541ef2ed25.jpg 245w," sizes="100vw" width="2419" height="946"><ul><li>Column dimensions: Watrex GPC/SEC columns are available in analytical (8x250 mm or 8x300 mm) and preparative configurations (20x300 mm).</li><li>Examples of use: Separation of synthetic polymers, peptides, proteins, polysaccharides.</li></ul><h3>Advantages of Watrex HPLC columns</h3>Columns manufactured directly in the Watrex laboratory provide several fundamental advantages:<ul><li>High quality at friendly prices. Thanks to the fact that they produce the columns themselves, they can offer top quality at very affordable prices.</li><li>A wide range of sizes and types. It offers columns adapted to various chromatographic techniques, including analytical and preparative applications.</li><li>Flexibility in applications. Watrex columns are compatible with a variety of mobile phases and can be used for the separation of ionic compounds, organic acids, polymers and biomolecules.</li></ul><h3>Conclusion</h3><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=48&amp;distributorItems=59&amp;manufacturerItems=93">Watrex HPLC and GPC/SEC columns </a>are a highly reliable solution for a wide range of chromatographic applications and combine high quality with a favorable price. Whether you need columns for reverse phase, ion exchange, ion exclusion, size exclusion chromatography (SEC) or gel permeation chromatography (GPC), Watrex products guarantee you excellent separation properties and reproducible results.For more information, feel free to contact Watrex <a href="(watrex@watrex.com">(watrex@watrex.com</a>) or visit their website (<a target="_blank" rel="noopener noreferrer" href="https://watrex.com/cs/uvod/?utm_source=labrulez">www.watrex.com</a>).

8 points on how to care for HPLC columns

<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">The Streamline™ Clarity data station</a> from the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/60">Czech company DataApex</a> is an ideal solution for efficient data acquisition and analysis.DataApex, like us, is based in the Czech Republic and boasts more than 30 years of experience in the field. Since its inception in 1991, it has become a recognized company in the market and their current Clarity product line is already the third generation of chromatography stations.They emphasize intuitive design, top quality and provide excellent technical support.DataApex products are loved by customers in more than 100 countries around the world, which confirms their high standard and reliability.For more information or to order, do not hesitate to contact us!<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Streamline_Clarity_78f92650cc.jpg" alt="Watrex: Streamline™ Clarity" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Streamline_Clarity_78f92650cc.jpg 245w," sizes="100vw" width="1024" height="500"><h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/pumps/gradient-pumps/streamline-binary-high-pressure-gradient-system-stainless-steel/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex Streamline binary gradient HPLC system</a></h4>Watrex Streamline™ binary gradient HPLC system consists of two P1 pumps, static mixer, CT1 column thermostat, PDA1 diode-array detector, AS1 auto-sampler, and Clarity PDA datastation.Streamline™ binary gradient HPLC system consists of two P1 pumps, static mixer, CT1 column thermostat, PDA1 diode-array detector, AS1 auto-sampler, and Clarity PDA datastation.<h3>FEATURES</h3><ul><li>Optimized pump mechanics with parallel piston design offers excellent flow rate accuracy and gradient composition at all solvents ratios</li><li>Variable volume injection range 0.1 - 999.9 μl, high precision and reproducibility, and low carryover AS1 autosapler</li><li>Excellent signal-to-noise ratio of the PDA2 detector</li><li>Data acquisition, processing and instrument control is by <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity Chromatography Station*</a></li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1000">DataApex Clarity Offline Chromatography Software</a></h4>Clarity Offline allows users to import previously acquired data and to evaluate and prepare methods in regulated environment.Clarity Offline allows users to import previously acquired data and to evaluate and prepare methods. This version cannot acquire data, but it can share data or even directly access (through LAN) projects of the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity</a> and Clarity Lite software. With Clarity Offline users are able to work with acquired data on additional computers in the lab or at home. The software works with the same files and provides the same outputs (printing and exporting) as Clarity and Clarity Lite.Clarity Offline is intended for use as additional software that supplements Clarity and Clarity Lite. It can also be used to evaluate data from other manufacturers' CDS.<blockquote>Ask for <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/product/clarity-demo">Clarity Demo version</a> which allows you to get your hands on software before purchase</blockquote>&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_Chromatograficky_Software_f77d0405d2/DataApex-Clarity-Chromatograficky-Software.jpg" alt="DataApex Clarity Chromatografický Software.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Clarity_Chromatograficky_Software_f77d0405d2/thumbnail_DataApex-Clarity-Chromatograficky-Software.jpg 245w," sizes="100vw" width="800" height="487">

Streamline™ Clarity solution for HPLC

Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"><h4>The Role of Chromatography in Forensic Food Analysis</h4>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:<ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate adulteration.</li></ul>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.<h4>Isocratic vs. Gradient Elution</h4>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:<ul><li>Isocratic HPLC uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li>Gradient HPLC changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.<h4>Watrex Praha and the Contribution of Modern Systems to Food Analysis</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha, s.r.o.</a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline">Streamline™ product line</a> includes several HPLC system concepts suitable for food analysis:<ul><li>Isocratic HPLC system – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li>Binary gradient HPLC system – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures (e.g., phenolic profiles of wine, components of aromatic extracts).</li><li>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography) – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf">Product catalogue HERE</a></blockquote>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.<h4>Practical Examples of Adulteration Revealed by HPLC</h4><ul><li>Detection of olive oil dilution: HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li>Estimation of sugar addition in honey: Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li>Identification of aromatic adulterants in wine: Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4>Conclusion</h4>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.<blockquote>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy">Watrex Praha</a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true">LinkedIn</a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague">Facebook</a>) for more engaging topics.</blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

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WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

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