<h3>What is Samplify?</h3>Samplify is an innovative new technology from SIELC, specifically designed for unattended, routine, periodic sampling of virtually any liquid source. Each sample is transferred automatically into an individual destination vial or 96-well plate. Vial trays or plates may then be loaded directly into a variety of analytical systems for further processing.<blockquote>Precise, Reproducible, Unattended Sampling – from: Reactors, Fermenters, Dissolution Vessels, Process Streams, and other Liquid Sources.</blockquote><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Sampling_Solutions_Start_Here_Samplify_from_SIELC_Technologies_2_e6db88c004.jpg" alt="Bioanalytic: Samplify from SIELC Technologies." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Sampling_Solutions_Start_Here_Samplify_from_SIELC_Technologies_2_e6db88c004.jpg 127w," sizes="100vw" width="1012" height="1247">The Samplify system consists of two principal components:<ol><li>&nbsp;a sophisticated electro-mechanical sample probe constructed of materials providing chemical and temperature resistance, and</li><li>SIELC’s renowned and highly flexible liquid handler (LHS) for pulling samples from the liquid source and distributing samples into destination locations.</li></ol>The LHS offers many additional capabilities; for example, the addition of quenching reagents into sample vials immediately after acquisition, if required, and thorough probe cleaning to guard against cross-sample contamination. The entire system may be controlled with SIELC’s Samplify Windows app, or via serial port using provided commands.<blockquote>Samplify is ultra-compact, allowing placement of the probe – or the probe plus LHS, as required – in confined spaces such as a reaction hood or a refrigerator.</blockquote><h3>Samplify Specifications</h3><h4>Samplify Probe</h4><ul><li>Connection: requires Samplify LHS</li><li>Small dimensions: 11 x 1 inch, DIA 1/4 inch. Immersion length up to 6”</li><li>Contact with liquid: Stainless Steel 316 (316L), PEEK &amp; PTFE</li><li>Variable sample volume: from 5 to 500 μL</li><li>Max. working pressure: 120 psi (8 bar)</li><li>Max. working temperature: 200 °C</li></ul><h4>Samplify LHS</h4><ul><li>Instrument Size: 6 x 6.5 x 7 inch (15 x 16 x 17 cm)</li><li>Weight: 5 lb (2.3 kg)</li><li>Collection Options: 48 vial plate (2 mL vials); 96 well plate</li><li>Pressure Max: 250 psi/ 14 bar (4000 μL volume)</li><li>Syringe Capacity Options: 4000 μL</li><li>Valve Options: 7x6</li><li>Volume Accuracy: +/-1 μL (4000 μL volume)</li><li>Probe dead volume: 767 μL</li><li>Syringe draw/refill rates: 48,000 μL/min</li><li>Communication: USB (type B) COM port</li><li>Power: 24 V DC</li><li>Contact with liquid: Stainless Steel 316 (316L), PEEK, PTFE, Vespel</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Sampling_Solutions_Start_Here_Samplify_from_SIELC_Technologies_Samplify_TM_Probe_3c4d643d62.jpg" alt="Bioanalytic: Samplify from SIELC Technologies - Probe." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Sampling_Solutions_Start_Here_Samplify_from_SIELC_Technologies_Samplify_TM_Probe_3c4d643d62.jpg 108w," sizes="100vw" width="1299" height="1867"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Sampling_Solutions_Start_Here_Samplify_from_SIELC_Technologies_Samplify_TM_LHS_e16e757cd9.jpg" alt="Bioanalytic: Samplify from SIELC Technologies - LHS." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Sampling_Solutions_Start_Here_Samplify_from_SIELC_Technologies_Samplify_TM_LHS_e16e757cd9.jpg 168w," sizes="100vw" width="859" height="799">

Sampling Solutions Start Here: Samplify from SIELC Technologies

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 11th November 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you applications and other documents by SIELC, Thermo Fisher Scientific, Shimadzu, Waters Corporation, and Agilent Technologies!<h4>1. SIELC: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/241113_Samplify_Brochure_367aa5da2e.pdf">Samplify from SIELC Technologies</a></h4><ul><li>Brochure</li></ul><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/4709">What is Samplify</a>?Samplify is an innovative new technology from SIELC, specifically designed for unattended, routine, periodic sampling of virtually any liquid source. Each sample is transferred automatically into an individual destination vial or 96-well plate. Vial trays or plates may then be loaded directly into a variety of analytical systems for further processing.The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1280">Samplify</a> system consists of two principal components:<ol><li>&nbsp;a sophisticated electro-mechanical sample probe constructed of materials providing chemical and temperature resistance, and</li><li>SIELC’s renowned and highly flexible liquid handler (LHS) for pulling samples from the liquid source and distributing samples into destination locations.</li></ol>The LHS offers many additional capabilities; for example, the addition of quenching reagents into sample vials immediately after acquisition, if required, and thorough probe cleaning to guard against cross-sample contamination. The entire system may be controlled with SIELC’s Samplify Windows app, or via serial port using provided commands.Samplify SpecificationsSamplify Probe<ul><li>Connection: requires Samplify LHS</li><li>Small dimensions: 11 x 1 inch, DIA 1/4 inch. Immersion length up to 6”</li><li>Contact with liquid: Stainless Steel 316 (316L), PEEK &amp; PTFE</li><li>Variable sample volume: from 5 to 500 μL</li><li>Max. working pressure: 120 psi (8 bar)</li><li>Max. working temperature: 200 °C</li></ul>Samplify LHS<ul><li>Instrument Size: 6 x 6.5 x 7 inch (15 x 16 x 17 cm)</li><li>Weight: 5 lb (2.3 kg)</li><li>Collection Options: 48 vial plate (2 mL vials); 96 well plate</li><li>Pressure Max: 250 psi/ 14 bar (4000 μL volume)</li><li>Syringe Capacity Options: 4000 μL</li><li>Valve Options: 7x6</li><li>Volume Accuracy: +/-1 μL (4000 μL volume)</li><li>Probe dead volume: 767 μL</li><li>Syringe draw/refill rates: 48,000 μL/min</li><li>Communication: USB (type B) COM port</li><li>Power: 24 V DC</li><li>Contact with liquid: Stainless Steel 316 (316L), PEEK, PTFE, Vespel</li></ul><h4>2. Agilent Technologies: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_ultrafast_immunosuppressants_lc_ms_5994_7829en_agilent_414e1b7386.pdf">Ultrafast Quantitation of Immunosuppressant Drugs in Whole Blood by Agilent 6475 LC/MS</a></h4><ul><li>Application</li></ul>AbstractAn ultrafast, 2.5-minute quantitation method using liquid chromatography/mass spectrometry (LC/MS) for four immunosuppressant drugs was established using an Agilent 6475 triple quadrupole LC/MS with the RECIPE therapeutic drug monitoring (TDM) kit. This application note emphasizes validation of an ultrafast measurement of tacrolimus, sirolimus, everolimus, and cyclosporine A in whole blood with outstanding sensitivity and interday reliability.IntroductionTDM is essential for ensuring efficacy and minimizing the adverse effects of drug delivery. The need for immunosuppressant drugs for organ and bone marrow transplant patients has been growing annually. Administration of immunosuppressants is strictly monitored as many side effects, including nephrotoxicity and neurotoxicity, must be considered. LC/MS is considered the gold standard for measurement of immunosuppressants due to results showing remarkable sensitivity and specificity.The RECIPE ClinMass TDM kit system for immunosuppressants in whole blood (Munich, Germany) was used on the 6475 triple quadrupole LC/MS for analysis of tacrolimus, sirolimus, everolimus, and cyclosporine A. Validation of the RECIPE TDM kit was implemented to evaluate sensitivity, linearity, and interday repeatability across three consecutive days (n = 3). Sample preparation is straightforward without the need for complicated cleanup or filtration.ConclusionA rapid analysis of tacrolimus, sirolimus, everolimus, and cyclosporine A in whole blood was established on an Agilent 6475 triple quadrupole LC/MS. Together with straightforward sample preparation, the method demonstrates robust measurement of immunosuppressant drugs in biological samples. Ultrafast acquisition times allow the high-throughput analysis of more than 500 samples per day. By coupling with the ready-to-use RECIPE ClinMass TDM kit system, Agilent provides a comprehensive end-to-end solution for therapeutic drug monitoring (TDM) of immunosuppressants.<h4>3. Thermo Fisher Scientific: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_003318_lsms_mabs_orbitrap_ascend_biopharma_tn003318_na_en_180dc41f10.pdf">In-depth characterization of monoclonal antibodies</a></h4><ul><li>Application</li></ul>GoalTo assess the performance of the Thermo Scientific™ Orbitrap™ Ascend BioPharma Tribrid™ mass spectrometer for comprehensive monoclonal antibody (mAb) characterization using intact mass analysis and middle-down mass spectrometry (MD-MS) approaches. The MD-MS methods were developed using a combination of powerful ion activation techniques including electron transfer dissociation (ETD), electron transfer higher energy collision dissociation (EThcD), ultraviolet photodissociation (UVPD), and ion manipulation by proton-transfer charge reduction (PTCR).IntroductionmAbs can display structural heterogeneity due to the presence of various post-translational modifications (PTMs), such as glycosylation, oxidation, and deamidation. These modifications can affect their structure, stability, and function. Therefore, in-depth characterization of mAb heterogeneity is critical to ensure the safety, efficacy, and quality of biotherapeutics. The middle-down mass spectrometry (MD-MS) approach has emerged as a promising tool for therapeutic characterization.1-4 It involves the formation of mAb subunits by reduction and/or digestion, followed by tandem MS (MS/MS) fragmentation of these subunits, offering high sequence coverage while involving a straightforward sample preparation.The Orbitrap Ascend BioPharma Tribrid mass spectrometer offers many benefits for biopharmaceutical characterizations. The high mass accuracy and high resolution offered by the Orbitrap technology enable precise mass measurement of intact mAbs or subunits. The Orbitrap Ascend BioPharma MS provides multiple MS/MS fragmentation techniques, including collision-induced dissociation (CID), higher-energy collisional dissociation (HCD), ETD, EThcD, electron transfer and collision-induced dissociation (ETciD), and UVPD, to achieve a full characterization of mAbs. In addition, this powerful instrument offers the PTCR technology with extended mass ranges (up to 16,000 m/z with Native MS option enabled) that can be employed alone or combined with different fragmentation techniques for the characterization of complex biotherapeutics. The Orbitrap Ascend BioPharma MS also benefits from its unique revolutionary architecture by having a second ion-routing multipole (front HCD cell) in the system which improves the transmission of large molecules and increases the sensitivity to detect low-abundance species in complex samples.In this work, intact mass analysis and MD-MS approaches were employed to characterize trastuzumab and its subunits on an Orbitrap Ascend BioPharma Tribrid MS. All the analyses were performed in the intact protein mode at various pressure modes, and for intact trastuzumab the Native MS option was used. Figure 1 shows the main LC-MS workflow employed for intact and subunit analyses of trastuzumab.ConclusionIn this work, intact and middle-down workflows were employed to obtain precise intact mass and high sequence coverage of mAb subunits using the Vanquish Flex UHPLC system and Orbitrap Ascend BioPharma Tribrid mass spectrometer. The Orbitrap Ascend BioPharma MS provides the following advantages for comprehensive biopharmaceutical characterization.<ul><li>The high mass accuracy and high resolution offered by the Orbitrap technology provides precise mass measurement for both intact mAbs (native and denaturing) and their subunits.</li><li>The system provides multiple fragmentation techniques, such as ETD, EThcD, and UVPD, for achieving high sequence coverage for various biopharmaceutical molecules.</li><li>Compared to ETD, EThcD and UVPD provide an improved sequence coverage of mAb subunits due to the formation of additional fragment types.</li><li>The combination of PTCR with EThcD and UVPD led to further increase in sequence coverage of mAb subunits and the number of complementary ion pairs.</li><li>The combination of EThcD, UVPD, and PTCR offers a nearly complete sequence coverage (&gt;90%) for subunits in the size of 20–25 kDa (Fc/2, LC, and Fd’ subunits) and high sequence coverage (&gt;70%) for the HC subunit (≈50 kDa).</li></ul><h4>4. Shimadzu: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00814_en_028966d0aa.pdf">Automatic Optimization of Gradient Conditions by AI Algorithm on Synthetic Peptide and Impurities</a></h4><ul><li>Application</li></ul>User Benefits<ul><li>The AI algorithm of LabSolutions MD can automatically optimize gradient conditions to greatly reduce labor of LC method development.</li><li>Anyone can optimize gradient conditions, regardless of their experience in chromatography.</li><li>Gradient conditions that meet the resolution criteria for specified peaks are automatically searched (e.g., principal component and related impurities).</li></ul>IntroductionIn the typical LC method development, the process begins with “preparation” which includes mobile phase preparation, column installation, and creation of analysis schedules, then the analysis is started. After that, the acquired data is analyzed and “preparation” for the subsequent analysis is carried out, followed by starting the next analysis again. The method development progresses by repeating these processes, but in addition to the significant time required to repeatedly create analysis schedules, expertise in chromatography is necessary to explore optimal conditions based on data analysis. In other words, typical method development requires “human intervention”. Therefore, eliminating human involvement and automating such method development processes would be desirable to improve labor efficiency. This article introduces an example of automatic optimization of gradient conditions to meet resolution criteria for synthetic peptide and related impurities using LabSolutions MD (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/c190_e309_96044bc345/c190-e309.pdf">Technical Report C190-E309</a>), a dedicated software for supporting method development.ConclusionAutomatic optimization of gradient conditions using AI algorithm of LabSolutions MD was applied to synthetic peptide and related impurities. As a result, gradient conditions that met the criteria (resolution for FLP &gt; 2.0) were successfully explored. This result indicates that significant labor saving in method development can be expected by LabSolutions MD. This article introduces an automatic optimization of gradient conditions in method development while LabSolutions MD also supports a series of workflow of method development, including screening of mobile phases and columns. For details, please refer to the application news, “<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00780_en_4d0aa014ea.pdf">Efficient Method Development for Synthetic Peptide and Related Impurities (01-00780)</a>”.<h4>5. Waters Corporation / <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/303">HPLC</a>: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/hplc_2024_gauthier_mixers_d9bc1d801d.pdf">COMPARISON OF MIXER PERFORMANCE FOR HPLC METHODS UTILIZING TFA GRADIENTS</a></h4><ul><li>Poster (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/303">HPLC</a>)</li></ul>INTRODUCTIONSolvent mixing is critical to obtaining optimal LC separations. Currently, there are two commonly available pump mixing designs for reversed phase gradient HPLC separations - high pressure (typically binary) and low pressure (all quaternary) systems. High pressure systems use two independent pumps to deliver different solvents. Mixing occurs at high pressures with solvent composition controlled by the flow rates of the two pump heads. In low pressure systems, solvent composition is controlled by a gradient proportioning valve. Each solvent is delivered in packets (based on the gradient specified in the method) which are mixed as they go through the pump head. Both high and low pressure systems are subject to mobile phase composition fluctuations. Mixers of various volumes and designs are utilized to help minimize these composition fluctuations by reducing baseline noise and oscillations in mobile phase composition.Trifluoroacetic acid (TFA) is a commonly used ion-pairing reagent used in combination with acetonitrile for many gradient reversed phase applications. TFA absorbs strongly at wavelengths below 250 nm. Additionally, TFA is slightly retained on reversed phase columns which results in fluctuations in TFA concentration as the acetonitrile gradient passes through the column. In combination, these factors result in significant baseline disturbances (ripples) when TFA - acetonitrile gradients are used at low wavelengths. These baseline ripples may make peak integration difficult, and can impact the sensitivity and retention time precision of the method.Most modern HPLC systems include a standard mixer in the pump design. Additionally, different mixers may be available to improve mixing performance for specific applications. In this study, mixers of varying volumes and design were evaluated.CONCLUSIONMixing performance is critical to obtaining optimal LC separations. In this study, several HPLC systems and mixers were evaluated using a TFA-Acetonitrile gradient method at a low wavelength which is known to produce baseline ripples. The USP signal to noise ratio was used to determine the impact of the different mixers.The results demonstrate that the sensitivity of the method is impacted by the system/mixer used, and consideration should be given to mixer selection, especially when running methods which are subject to increased baseline noise.

News from LabRulezLCMS Library - Week 46, 2024

<h4>A New Standard in HPLC Column Connection by SIELC</h4>SIELC Technologies, a pioneer in chromatography innovation, introduces a single-ended HPLC column — a revolutionary new design that redefines how columns are connected and used in high-performance liquid chromatography. For decades, laboratories have relied on the same standard column format — and have dealt with the same recurring problems: leaky fittings, clogged junctions, dead volume, and the constant hassle of tubing and connection selection.SIELC has developed a single-ended HPLC column design.This new system redefines how columns are installed, used, and replaced — streamlining your workflow and eliminating common points of failure.<h5>Key Benefits of the New Design:</h5><ul><li>No fittings or tubing required — eliminates leaks, complex installation, and the need to match tubing length or ID.</li><li>Zero dead volume thanks to a direct connection via a dedicated adapter.</li><li>Leak containment — in case of pressure failure, solvent remains inside the adapter with no external leakage.</li><li>Tool-free column replacement — disconnect the column without touching the fittings.</li><li>Aluminum body — ensures efficient heat transfer and provides a smooth, label-friendly surface even on the shortest columns.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_HPLC_column_3af7eb512a.jpg" alt="SIELC: HPLC column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_HPLC_column_3af7eb512a.jpg 171w," sizes="100vw" width="1600" height="1459"><h4>HPLC Column Adapter</h4>The SIELC adapter is a key component of the new single-ended column system, designed to eliminate leaks, dead volume, and connection complexity in HPLC setups.This adapter is an engineering solution that ensures perfect sealing, precise flow alignment, and reliable performance under high pressure — all without traditional fittings, ferrules, or tools.<ul><li>Dimensions: 21 × 21 × 26 mm (0.83 × 0.83 × 1.02 in)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_HPLC_Column_Adapter_8495244726.jpg" alt="SIELC: HPLC Column Adapter" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_HPLC_Column_Adapter_8495244726.jpg 165w," sizes="100vw" width="918" height="868"><blockquote>Special offer: First-time buyers receive a Free Single-Column Adapter with their purchase. Experience the new connection standard — no fittings, no leaks, no extra cost.</blockquote><h4>How the Single-Ended HPLC Column Works?</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_How_the_Single_Ended_HPLC_Column_Works_24ebf8b072.jpg" alt="SIELC: How the Single-Ended HPLC Column Works?" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_How_the_Single_Ended_HPLC_Column_Works_24ebf8b072.jpg 245w," sizes="100vw" width="1600" height="474">As the mobile phase enters through the MP Inlet, it flows into the adapter’s internal capillary aligned with the column axis. When the mobile phase passes through the column packing bed, it exits through the bypass channel connected with the MP Outlet.This design eliminates dead volume, prevents mobile-phase leakage, and provides a simple way to remove and replace the column when needed.<h3>Dual-Ended vs. Single-Ended HPLC Columns</h3><h4>Dual-Ended HPLC Columns</h4><ul><li>Column connections: Dual-ended HPLC columns have two separate ends. Each connection requires fittings, ferrules, and tubing to connect the column to the LC system.</li><li>Installation: Installation requires manual alignment of fittings and precise adjustment of tubing length to minimize dead volume. This process can be time-consuming and is prone to user error.</li><li>Dead volume: Dead volume is typically present at both the inlet and outlet connections. These junctions can cause peak broadening and reduced chromatographic efficiency.</li><li>Leakage risk: Leakage risk is relatively high because solvent connections rely on ferrules and fittings that may loosen or wear over time.</li><li>System integration: Dual-ended columns require separate tubing and careful fitting when installed in column heaters or coolers.</li><li>Thermal performance: Heat transfer may be less uniform due to multiple connections and tubing interfaces.</li><li>Guard column option: Using a guard column requires additional hardware and extra connections, which can introduce additional dead volume into the system.</li><li>Available phases: Dual-ended columns are typically available in standard stationary phases such as C18, HILIC, ion exchange, and others.</li></ul><h4>Single-Ended HPLC Columns</h4><ul><li>Column connections: Single-ended columns use a single coaxial connection with an integrated adapter. No fittings, ferrules, or tubing are required.</li><li>Installation: The adapter is installed only once. After that, the column can be replaced in seconds without tools, making maintenance faster and simpler.</li><li>Dead volume: Near-zero dead volume is achieved because the internal capillaries are precisely aligned within the adapter.</li><li>Leakage risk: The mobile phase is fully contained inside the adapter, creating a leak-free design even under high pressure conditions.</li><li>System integration: A compact stainless-steel adapter allows easy installation in column heaters or coolers.</li><li>Thermal performance: The aluminum outer body provides efficient and uniform heat transfer across the column.</li><li>Guard column option: An integrated zero-dead-volume guard column option is available, allowing quick cartridge replacement without tools.</li><li>Available phases: All SIELC phases as well as traditional chromatographic phases (C18, HILIC, ion exchange, mixed-mode, BIST™, etc.) are available in this format.</li></ul><h4>Adapter for Guard Column</h4>If your workflow requires a guard column or a filter to be installed inline with the main column, then a specialized adapter with a guard option should be used.The SIELC HPLC Single-Column Guard protects the analytical column by capturing particulates and strongly retained contaminants before they reach the stationary phase. Its unique direct-insert design provides a zero-dead-volume connection and allows quick, tool-free replacement of a used guard with a new one. Guards are available for all SIELC stationary phase types — Primesep™, Newcrom™, Chromni™, PEI™, BIST™, and for generic phases such as C18, HILIC and IE.<h5>Guard Cartridges (2 per pack)</h5><ul><li>Guard Adapter — Dimensions: 21 × 21 × 42 mm (0.83 × 0.83 × 1.65 in)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_guard_cartridges_4077d1edf2.jpg" alt="SIELC: Guard cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_guard_cartridges_4077d1edf2.jpg 245w," sizes="100vw" width="768" height="262"><h4>Why This Matters</h4>This new column system removes the guesswork and fragility from high-pressure liquid chromatography. By eliminating traditional high-pressure fittings and minimizing dead volume, you gain more consistent results, lower the risk of system failure, and reduce downtime.Whether you’re running routine analyses or complex separations, this design enhances reliability and usability — so you can focus on your science, not your setup.<blockquote><h5>All SIELC and Traditional Columns and Guards Available in a New Format</h5>C18, HILIC, Ion Exchange, Mixed-Mode, Ion Exclusion, Protein, BIST, and more.</blockquote><blockquote>📄 Want to learn more? <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Single_ended_column_new_2_1_b80be5f107.pdf">Download our brochure</a> to learn about a single-ended HPLC column design.</blockquote>

Single-Ended HPLC Column by SIELC

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