<p><strong>Prof. Ing. Michal Kohout, Ph.D. is originally from southern Bohemia. He started studying at UCT Prague in 2000 after high school in Soběslav. After his postdoctoral research at the Institut of Analytical Chemistry of the University of Vienna, he decided to return to his alma mater, UCT Prague, and extend, apply and transfer knowledge and experience he gained abroad. He was recently named full professor and, in addition to research and teaching, considers his mission as professor to be setting an example for students in terms of not being afraid to start one’s own business since he, with colleagues, founded a UCT Prague spin-off company, Galochrom, discussed below.</strong></p>
<h5><strong>What does your research group do? What is the focus of your research activities?</strong></h5>
<p>We have been dealing with liquid crystals for a very long time. Liquid crystals are organic materials with various applications, and as research evolves, it’s expected that liquid crystals will have some added value in real-life applications, beyond simply being liquid crystalline (e.g. being chiral, sensitive to light or to magnetic fields). We try to modify molecules so that they can handle the various demands that modern materials chemistry places on them.</p>
<p>The second area of focus, chiral stationary phases for liquid chromatography, I brought back with me to UCT Prague from my post-doc stay. In liquid chromatography, we study systems that are used in the analysis of drugs (if an active substance is chemically and optically pure), for example.</p>
<p>Together with my colleagues and students, we also work on various side projects in classical organic chemistry, such as organocatalysis.</p>
<h5><strong>Most people can only conceptualize solid crystals. Can you introduce us to the world of liquid crystals?</strong></h5>
<p>It’s quite simple: liquid crystals are basically crystals too. One can imagine a crystal as being an ordered and oriented structure with molecules precisely and unambiguously arranged in space. Liquid crystals also have these characteristics, but their molecules can also move and are mobile even though they have, let’s say, a partially-preserved crystalline structure, for they are in the so-called mesophase. To conceptualize this, one could simply compare this to a system of straws floating in the same direction, because the molecules are arranged like this and are able to move when acted upon by an external impulse (e.g. electric or magnetic field or light). A structure can be rearranged so that it occupies a new, lowest energy arrangement. Liquid crystals are used, for example, in optoelectric devices, and we’re all surrounded by liquid crystals, which are not just used in monitors, televisions, and watch displays, but also in soap or cell membranes. One doesn’t even realize how many liquid crystalline substances and systems actually are all around us in the real world.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Pokousime_se_uspet_se_spin_off_firmou_Galochrom_profesor_Michal_Kohout_e7be735e35.jpg" alt="UCT Prague: We are trying to succeed with the spin-off company Galochrom"/></p>
<h5><strong>Is there anything else you’re up to that you’d like to tell us about?</strong></h5>
<p>I’d put liquid chromatography into the background a bit. Let me put it this way: the world of liquid crystals research is a kind of long-distance race, and we don’t know for sure if and when relatively complex molecules will be used. They certainly have the potential to be used in real life, but in the distant future. It is not easy to prepare and enhance them, so that they meet various requirements and do as many extra things as we would like.</p>
<p>On the other hand, if we look at the area of chromatography, the real world application of new research results happens relatively quickly. If we prepare a chromatography column that is significantly better than what’s currently on the market, it might be commercially interesting and result in a product that we’ll see on the market in our lifetimes.</p>
<p>But it bothers me and my colleagues that even the development of unique stationary phases is still too slow, that it takes too long for a chromatographic column to reach the market. In 2017, we tried to approach several large manufacturers with a unique system we patented. Unfortunately, there was no interest in this technology because manufactures would have to change their production technologies slightly. Because of this, my colleagues and I decided to found our own company, the Galochrom, UCT Prague spin-off. This way, we also wish to show younger colleagues and students that they don’t have to be afraid of starting a company and entering the business world.</p>
<h5><strong>How is Galochrom doing?</strong></h5>
<p>We are in the early phases. We founded the spin-off in September 2023, and since then, we placed second in a European start-up competition, which we consider to be a great success. We are currently trying to put the first products on the market and prove that they are better than those already available. Of course, we believe that our products will be adopted.</p>
<h5><strong>What practical value do you think your research and professional activities have for society and your field?</strong></h5>
<p>Our multimodal columns will have a wider range of applications and will thus be able to replace three to four standard columns. This will lead to dramatic savings for end users as well as lower solvent and solvent disposal costs.</p>
<p>As for liquid crystals, they’re a long shot, as I’ve already said, but we might, for example, use a system of so-called dopants. Let’s take a photosensitive material. We don’t have to use it as a single component; instead, we can add it to a cheaper commercial material and mix it (5-10%). Thanks to the dopant, an entire system is sensitive to light and we can not only switch individual parts (pixels) from the mesophase to the liquid phase, but also read this information with a laser. Such systems could serve as optical memories.</p>
<h5><strong>You recently became a full professor. What does this mean for you? What do you teach and what are your first impressions as professor?</strong></h5>
<p>Of course, I consider this a great personal success built upon the great work and support by my students, colleagues, and mentors. My original motivation for entering academia, a long time ago, was a promise I made to my dad: that I would graduate as one of the best in my class at UCT Prague. And I’ve made it this far, so I think he would be proud of me and that fills me with a certain sense of fulfilment. I teach organic chemistry. So for first impressions: I have more work, but it’s interesting for me, nevertheless, to teach students who are just starting their university programs. My lectures before this were for advanced students and doctoral candidates. All of a sudden, when you’re teaching core subjects, you start trying not to influence students’ mind sets too much while still conveying the information in some absorbable way, reassuring them that chemistry is actually cool and that chemists are pretty normal people.</p>
<h5><strong>It must be quite challenging to capture the attention of an auditorium full of people and to decide how much to go into detail. Do you struggle with this kind of thing? Do you have any tricks for engaging your audience?</strong></h5>
<p>It’s true, when you enter an auditorium and there are 180 people sitting there instead of 20, it’s really an incredible difference. It’s not so much about the number of people itself, because there are about the same number of people at conference presentations, but conference attendees are experts who aren’t really surprised by anything in the field. Teaching core subjects is rather about setting some level of comprehensibility for what you’re explaining.</p>
<p>Gradually, I’m getting faster at preparing my lectures, but at first, it was very time-consuming. Yet, during the class, sometimes I still get taken away a bit and then I get behind and play catch up. (He laughs.)</p>
<p>As for student engagement, I tell my colleagues that I’m trying to use my latent theatre talents, so hope that&#39;s at least somewhat successful. I’m going back in time a couple of decades and am going to use some simple, themed experiments from back then in my lectures.</p>
<h5><strong>You started studying at UCT Prague in 2000. What do you think has changed the most for students since then?</strong></h5>
<p>Definitely the quality of the food in the canteens. One hundred percent! But what has really changed for the better are the travel possibilities. That&#39;s the biggest shift I see. Today, it’s easy to go abroad for a semester, almost whenever you want. There are countless ways to do this. I remember how I tried to do an internship during my doctoral studies, but preparations were crazy. So instead, I arranged at a conference with my mentor’s colleague to join her laboratory within a European project because it was so much easier to go this way. Going abroad gives you a wealth of new experiences, and it’s great that this option is much more accessible than before.</p>
<h5><strong>Is there anything that you did not like as a student and managed to change—or are at least trying to change—as a professor?</strong></h5>
<p>For a relatively long time, my colleagues and I tried to appeal to UCT Prague’s management to provide competitive scholarships for doctoral students. Doctoral candidates are the UCT Prague’s main strength because without them, the university couldn’t do basic research. They should be valued accordingly. So I’m a supporter of finding ways to increase scholarships and I think we’ve been quite successful in this regard.</p>
<h5><strong>What would you like to achieve in your professional and personal lives?</strong></h5>
<p>As a professional, I would like that the things we’ve invented would actually be used. Research, whatever form, should aim at developing things that are useful for humanity. Basic research is great, but I like finding applications while striving to advance both science and society as a whole. Why else would we be here?</p>
<p>Personally, my highest priority is work-life balance. Achieving some reasonable balance with work, what I like to do, and time for family.</p>
<h5><strong>What does the future hold for your research group?</strong></h5>
<p>The long-term plan involves spin-off companies and connecting academia with the commercial sector. Engagement in basic research is necessary for finding usable materials and applications, yet connecting these applications directly to the industrial sector is crucial for us. The plan is to try to make a platform for quick and simple technology transfer at UCT Prague.</p>
<h5><strong>What do you do when you turn out the lights in your lab?</strong></h5>
<p>I have hobbies I’ve done for a long time. I’m either going to volleyball or trying to go fishing. Fishing is more time-consuming, so I mainly stick to volleyball. And, of course, I’m running home to spend time with my loved ones.</p>


We are trying to succeed with the spin-off company Galochrom

<h3><strong>Chiral ION-QN &amp; Chiral ION-QD</strong></h3><p><i>Weak Anion-Exchanger (WAX) stationary phases for reliable chiral separation of acidic compounds</i></p><h4><strong>Transform Your Chiral Analysis</strong></h4><p>One of the main challenges of today’s liquid chromatography is chiral separation of ionised and ionisable analytes, e.g., organic acids, amino acids, N-protected amino acids, amino acid mimics, etc. The most efficient approach to purify and resolve such troublesome racemates utilises ion exchange-type stationary phases that facilitate strong interactions between the chiral selector and the analyte.</p><p>Galochrom introduces its own generic series of weak anion-exchangers, based on <i>tert</i>-butyl-carbamoylated Cinchona alkaloids quinine (QN) and quinidine (QD). These chiral phases have demonstrated excellent selectivity and improved peak resolution, resulting in highly accurate and replicable analyses. Our innovative manufacturing and strict quality control ensure that these generic columns not only outperform commercial alternatives, but also meet your technical requirements.</p><blockquote><p><span style="font-size:20px;"><strong>Chiral ION-QN &amp; Chiral ION-QD represent the most efficient approach to purify and resolve even the most challenging mixtures.</strong></span></p></blockquote><h4><strong>Applications</strong></h4><p>Chiral ION-QN &amp; Chiral ION-QD are ideal for enantioseparations of:</p><ul><li><strong>N-protected amino acids</strong></li><li><strong>Aminophosphonic &amp; Aminosulfonic acids</strong></li><li><strong>Lactic &amp; Thiolactic acids</strong></li><li><strong>Clenbuterol &amp; Thyroxin</strong></li></ul><h4><strong>Upgrade your separation performance and gain:</strong></h4><ul><li><strong>Premium enantioselectivity</strong></li><li>Elegant <strong>switch of the elution order of enantiomers</strong></li><li><strong>High-stability</strong> against all common HPLC solvents</li><li><strong>Seamless method transfer</strong></li></ul><h3><strong>Powerful Selectivity</strong></h3><p><i>Aim for the best resolution and selectivity possible</i></p><p>In collaboration with dozens of researchers, we have selected the best-performing derivatives of both quinine (QN) and quinidine (QD). As a result, the Chiral ION-QN features O-9-(tert-butylcarbamoyl)quinine, which is immobilized on fully-porous spherical silica (see below). The unique chiral scaffold of the cinchona backbone provides exceptional selectivity for wide array of acidic compounds.</p><p>The Chiral ION-QD features structurally similar selector to Chiral ION-QN. As a result, these selectors, while being diastereomers, act as pseudo-enantiomers. <strong>This feature enables elegant switch of elution order of enantiomers, which is often challenging for other chiral stationary phases.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Transform_Your_Chiral_Analysis_3_18e49ab8ee.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Transform Your Chiral Analysis." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Transform_Your_Chiral_Analysis_3_18e49ab8ee.jpg 241w," sizes="100vw" width="1908" height="1238"></p><h4><strong>Molecular recognition</strong></h4><p>The prime driving force for specific molecular association are electrostatic or ionic interactions. Once analyte reaches close proximity of the selector, weaker non-bonding interactions such as H-bonding, <span class="symbol">π</span>−<span class="symbol">π</span> stacking and steric interactions apply. These directive interactions result in different column retention per each enantiomer.</p><p><strong>Since analytes differ in combination and strength of these binding increments, our chiral stationary phase delivers superior molecular recognition.</strong></p><h4><strong>Supporting professionals across many industries</strong></h4><ul><li>Forensic Science</li><li>Chemical Manufacturing</li><li>Biotechnology</li><li>Agriculture</li><li>Academic Research&nbsp;</li><li>Pharmaceuticals</li><li>Food &amp; Quality Control</li></ul><h3><strong>Popular chiral columns</strong></h3><p><i>Essential tools with a wide range of applications</i></p><h4><strong>Find your best match</strong></h4><p>There are hundreds of chiral stationary phases available on the market. The selection of chiral HPLC columns below represents the most popular and top-performing choices for analysis and resolution of chiral acidic compounds. Displayed data are relevant to analytical and semi-preparative scale</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Transform_Your_Chiral_Analysis_4_5d48236baa.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Transform Your Chiral Analysis." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Transform_Your_Chiral_Analysis_4_5d48236baa.jpg 224w," sizes="100vw" width="1508" height="1052"></p><h4><strong>Take Full Advantage Of Unique Selectivity And Expand Your Research Possibilities</strong></h4><p>Chiral ION-QN &amp; Chiral ION-QD now available at <strong>ANALYTICAL &amp; PREPARATIVE</strong> scale</p><h3><strong>Order information</strong></h3><h4><strong>Analytical Columns</strong></h4><h5><strong>Chiral ION-QN</strong></h5><ul><li><strong>Dimensions [mm]: 150 x 3</strong></li><li>Porosity: [Å] 200</li><li>Cat. No.: ION01-A150</li><li><strong>Price [€]: 1 300</strong></li></ul><h5><strong>Chiral ION-QD</strong></h5><ul><li><strong>Dimensions [mm]: 150 x 3</strong></li><li>Porosity: [Å] 200</li><li>Cat. No.: ION02-A150</li><li><strong>Price [€]: 1 300</strong></li></ul><p><span style="font-size:12px;">*As an alternative, we can also offer 150x3 mm, 3 μm with 120 Å</span></p><h4><strong>Preparative Columns</strong></h4><h5><strong>Chiral ION-QN</strong></h5><ul><li><strong>Dimensions [mm]: 150 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION03-P150</li><li><strong>Price [€]: 9 800</strong></li></ul></li><li><strong>Dimensions [mm]: 250 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION04-P250</li><li><strong>Price [€]: 11 300</strong></li></ul></li></ul><h5><strong>Chiral ION-QD</strong></h5><ul><li><strong>Dimensions [mm]: 150 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION05-P150</li><li><strong>Price [€]: 9 800</strong></li></ul></li><li><strong>Dimensions [mm]: 250 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION06-P250</li><li><strong>Price [€]: 11 300</strong></li></ul></li></ul><blockquote><h3><strong>SPECIAL OFFER 1+1</strong></h3><p><span style="font-size:22px;"><strong>Buy 1 preparative column and get analytical column for FREE</strong></span></p><p>You can choose 150x4 or 250x4 column with the same sorbent as your preparative column.</p></blockquote>

Chiral ION-QN & Chiral ION-QD HPLC columns - Transform Your Chiral Analysis

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 16th September 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring to you applications, technical notes, posters, and other documents by <strong>Agilent Technologies</strong>, <strong>Shimadzu</strong>, <strong>Thermo Fisher Scientific,</strong> <strong>Waters Corporation</strong>,<strong> Metrohm</strong>, and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/313"><strong>Galochrom</strong></a>!</p><h4><strong>1. Galochrom: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Chiral_ION_QN_and_Chiral_ION_QD_Brochure_10c44e1448.pdf"><strong>Galochrom Chiral ION-QN &amp; Chiral ION-QD</strong></a></h4><ul><li>Brochure</li></ul><h5><strong>Weak Anion-Exchanger (WAX) stationary phases for reliable chiral separation of acidic compounds</strong></h5><p><strong>Transform Your Chiral Analysis</strong></p><p>Weak Anion-Exchanger (WAX) stationary phases for reliable chiral separation of acidic compounds One of the main challenges of today’s liquid chromatography is chiral separation of ionised and ionisable analytes, e.g., organic acids, amino acids, N-protected amino acids, amino acid mimics, etc. The most efficient approach to purify and resolve such troublesome racemates utilises ion exchange-type stationary phases that facilitate strong interactions between the chiral selector and the analyte.</p><p>Galochrom introduces its own generic series of weak anion-exchangers, based on tert-butyl-carbamoylated Cinchona alkaloids quinine (QN) and quinidine (QD). These chiral phases have demonstrated excellent selectivity and improved peak resolution, resulting in highly accurate and replicable analyses. Our innovative manufacturing and strict quality control ensure that these generic columns not only outperform commercial alternatives, but also meet your technical requirements.</p><p><strong>Chiral </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1241"><strong>ION-QN</strong></a><strong> &amp; Chiral </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1240"><strong>ION-QD</strong></a><strong> represent the most efficient approach to purify and resolve even the most challenging mixtures.</strong></p><h5><strong>Applications</strong></h5><p>Chiral ION-QN &amp; Chiral ION-QD are ideal for enantioseparations of:</p><ul><li><strong>N-protected amino acids</strong></li><li><strong>Aminophosphonic &amp; Aminosulfonic acids</strong></li><li><strong>Lactic &amp; Thiolactic acids</strong></li><li><strong>Clenbuterol &amp; Thyroxine</strong></li></ul><h4><strong>2. Metrohm: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_eis_004_download_4e13c9c1a9.pdf"><strong>Electrochemical Impedance Spectroscopy - Equivalent circuit models</strong></a></h4><ul><li>Technical note</li></ul><h5><strong>Electrochemical Impedance Spectroscopy</strong></h5><p><strong>Part 4 – Equivalent circuit models</strong></p><p>The circuit elements which are described in Application Note AN-EIS-003 can be combined in series and in parallel to build equivalent circuit models which can then be used to model the various phenomena occurring at the electrochemical interface.</p><p>This seven-part series introduces EIS and covers basic theory, experimental setups, common equivalent circuits used for fitting data, and tips for improving the quality of the measured data and fitting. This Application Note (part 4) provides example equivalent circuit models produced from the elements discussed in part 3. These models are among the most commonly encountered within electrochemistry research. The associated Nyquist plot is also shown for each model discussed.</p><p><strong>CONCLUSIONS</strong></p><p>This Application Note shows how electrical elements can be arranged in order to build both simple and more complex equivalent circuits that can be used for fitting EIS data. The resulting Nyquist plots for all the equivalent circuits are shown as well.</p><h4><strong>3. Thermo Fisher Scientific: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/wp_002641_ic_dionex_inuvion_ionic_small_polar_compounds_wp002641_na_en_9c0213a96b.pdf"><strong>Developing an ion chromatography system</strong></a></h4><ul><li>Technical note</li></ul><p><strong>Introduction</strong></p><p>Ion chromatography (IC) has become essential for analyzing ionic and small polar compounds. It has been applied in many areas, ranging from the analysis of drinking water, food and beverages, and cooling water to lithium-ion battery research, process quality assurance, and the presence of corrosives in liquified petroleum gas.</p><p>IC can trace its origins to work done at what would become Dionex Corporation in the mid-1970s with the introduction of the first ion chromatograph in 1975, the Dionex™ Model 10 IC system. This system had the essential elements of an IC system: a pump, separating column, eluent suppressor, conductivity detector, and data recorder (Figure 1).</p><p>This basic system provided adequate data, but several factors restricted sample throughput and required a considerable investment in labor and significant expertise to maintain. Column capacity and packed bed suppressor capacity were limited, and the instrument configuration was inflexible, constraining the type of samples that could be analyzed. Offline suppressor column regeneration was needed, the pressure tolerance was low, and the background conductivity was comparatively high. Furthermore, manual peak integration was required, eluent usage was significant, and there was substantial run-to-run variability. Since the Dionex Model 10 IC system, many innovations have been developed and incorporated into new systems to address these deficiencies. Additional advancements have enhanced robustness, reliability, user-friendliness, functional flexibility, and integrated data analysis. Ideally, an ion chromatograph will meet current customer demands while being adaptable to address potential future analytical needs.</p><p><strong>Conclusion</strong></p><p>The progression from the Dionex Model 10 IC system to the sophisticated Dionex Inuvion IC system highlights the significant advancements that have been made in the field of IC. These advancements have not only addressed previous limitations but also introduced new features and capabilities that significantly enhance the performance, reliability, and user-friendliness of IC systems. As a result, the Dionex Inuvion IC system represents the current pinnacle in the ongoing development of IC technology, setting a new standard for what can be achieved in the analysis of ionic and small polar compounds.</p><p>The Dionex Inuvion IC system is the result of state-of-the-art techniques and processes used to manufacture, design, and optimize essential hardware components such as pumps, instrument electronics, range-free detectors, and conductivity cells. This integration of advanced technology has resulted in a system that is not only reliable and robust but also flexible and user-friendly.</p><p>As we look to the future, the field of ion chromatography will continue to evolve with new challenges and opportunities on the horizon. The Dionex Inuvion IC system is well-positioned to meet these challenges, providing laboratories with a reliable and efficient solution for their analytical needs. In conclusion, the Dionex Inuvion IC system represents a significant achievement in ion chromatography, solidifying Thermo Fisher Scientific's position as a leader in the industry.</p><h4><strong>4. Waters Corporation: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/2024_asms_alelyunas_cellculturemediabioprocessing_27380a2953.pdf"><strong>A High Throughput LC-MS Method for Cell Culture Media Nutrient and Metabolite Analysis Supporting Upstream Bioprocessing</strong></a></h4><ul><li>poster / <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/296"><strong>ASMS</strong></a></li></ul><p><strong>INTRODUCTION</strong></p><p>A 9-minute rapid and direct liquid chromatography and mass spectrometry (LC-MS) analysis method for cell culture media (CCM) nutrients and metabolites covering 220+ compounds is described. The method is based on reversed phase chromatography where amino acids are directly detected without derivatization. This rapid method reduces turn-around time for monitoring and decision making using previously established user-friendly data review workflows and access to multivariate data analytics (MVDA). The method has been applied for the qualitative and quantitative determination of cell culture media nutrient and metabolite analysis in commercial cell culture media and spent media from process optimization in cNISTmAb production using NISTCHO cells. Monitoring of glucose directly from media using this method was also investigated and described.</p><p><strong>CONCLUSION</strong></p><ul><li>A rapid throughput method based on 9 min data acquisition is described for cell culture media nutrient and metabolite monitoring.</li><li>The method uses less than 10 μL of spent media sample and has coverage of 220 compounds.</li><li>Comparison with the previous 20 min method suggests the same high-quality data and method robustness, even when the analysis time is shortened by 50%.</li><li>Combination of the rapid throughput method with Waters bioprocess walk-up solution<sup>2</sup> can enable analytical scientist and bioprocess engineers to obtain high quality data easily and routinely for upstream bioprocess optimization.</li></ul><h4><strong>5. Shimadzu: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00767_en_135939ebe6.pdf"><strong>Automatic Optimization of Gradient Conditions by AI Algorithm for Impurity Analysis</strong></a></h4><ul><li>Application</li></ul><p><strong>User Benefits</strong></p><ul><li>The AI algorithm of LabSolutions MD can automatically optimize gradient conditions to greatly reduce labor of LC method<br>development.</li><li>Anyone can optimize gradient conditions, regardless of their experience in chromatography.</li><li>Gradient conditions that meet the resolution criteria for specified peaks are automatically searched (e.g., principal component and its related impurities).</li></ul><p><strong>Introduction</strong></p><p>In the typical LC method development, the process begins with “preparation” which includes mobile phase preparation, column installation, and creation of analysis schedules, then the analysis is started. After that, the acquired data is analyzed and “preparation” for the subsequent analysis is carried out, followed by starting the next analysis again. The method development progresses by repeating these processes, but in addition to the significant time required to repeatedly create analysis schedules, expertise in chromatography is necessary to explore optimal conditions based on data analysis. In other words, typical method development requires “human intervention”. Therefore, eliminating human involvement and automating such method development processes would be desirable to improve labor efficiency. This article introduces an example of automatic optimization of gradient conditions to separate montelukast (a small molecule drug) and its related impurities using LabSolutions MD (<a target="_blank" rel="noopener noreferrer" href="https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/technical/technical_reports/22105/c190-e309.pdf">Technical Report C190-E309</a>), a dedicated software for supporting method development.</p><p><strong>Conclusion</strong></p><p>Automatic optimization of gradient conditions using AI algorithm of LabSolutions MD was applied to montelukast and its related impurity. As a result, gradient conditions that met the criteria (“resolution for montelukast and related impurity &gt; 3.0” and “elution time of the last peak &lt; 15 minutes”) were successfully explored. This result indicates that significant labor saving in method development can be expected by LabSolutions MD. This article introduces an automatic optimization of gradient conditions in method development while LabSolutions MD also supports a series of workflow of method development, including the screening phase and robustness evaluation phase. For details, please refer to the Technical Report “<a target="_blank" rel="noopener noreferrer" href="https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/technical/technical_reports/14629/c190-e284.pdf">Efficient Method Development Based on Analytical Quality by Design with LabSolutions MD Software (C190-E284)</a>”.</p><h4><strong>6. Agilent Technologies: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_polar_nitrosamines_1260_infinity_ii_multisampler_5994_7589en_agilent_22cb1563ee.pdf"><strong>Improved LC/MS Performance to Determine Polar Nitrosamines Using the Agilent 1260 Infinity II Hybrid Multisampler</strong></a></h4><ul><li>Application</li></ul><p><strong>Abstract</strong></p><p>This application note demonstrates the use of the Agilent 1260 Infinity II Hybrid Multisampler for the analysis of polar nitrosamines injected from organic solvents of high elution strength. The 1260 Infinity II Hybrid Multisampler is used under optimized feed speed conditions to trap and enrich the polar nitrosamines on the column. This avoids breakthrough and provides better peak shapes with more reliable quantification and improved detection limits.</p><p><strong>Introduction</strong></p><p>With the chemical synthesis of active pharmaceutical ingredients (APIs), impurities occur during chemical reactions. Genotoxic impurities (GTIs) are especially important because of their carcinogenic potential. To control the amount of GTIs, the U.S. Food and Drug Administration and the European Medicine Agency released guidelines for the maximum intake of GTIs with the daily dose of a drug depending upon the duration of application. According to these guidelines, the exposure to an individual genotoxic impurity must be below 1.5 μg/day for an application time longer than ten years.<sup>1</sup> In 2018, the drug Diovan was withdrawn from the market due to an increased amount of the nitrosamine impurity N-nitrosodimethylamine (NDMA) in the included API valsartan.<sup>2,3</sup> This application note describes the results achieved by the Agilent 1260 Infinity II hybrid injector for the analysis of nitrosamines injected from high organic solvents. The peak performance, like area precision, linearity, and sensitivity, will be discussed.</p><p><strong>Conclusion</strong></p><p>This application note describes the comparative measurement of nitrosamines using the Agilent 1260 Infinity II Hybrid Multisampler in feed injection mode and classical flow through injection mode. With the application of an optimized feed injection method, highly polar nitrosamines in a solvent of high eluting strength can be separated with good peak shapes. With classical flow through injection, massive peak broadening or even breakthrough were observed. Peak area RSD and LOQs are typically lower by a factor of 3 to 5 with feed injection.</p>

News from LabRulezLCMS Library - Week 38, 2024

<h3><strong>Method Development</strong></h3><p><i>Develop robust methods that are easily transferable across laboratories</i></p><h4><strong>Engineered for reliable method development</strong></h4><p>Successful methods rely on results that can tolerate minor variations in chromatographic parameters. It is very important<br>to have proper tools to easily and quickly develop robust methods. Chiral ION-QN and Chiral ION-QD are designed to<br>provide you with accurate and consistent results, that are transferable across different LC system platforms. You can<br>rapidly develop methods in several chromatographic modes:</p><ul><li><strong>Normal-phase</strong></li><li><strong>Reversed-phase</strong></li><li><strong>Polar organic</strong></li><li><strong>SFC</strong></li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1241"><strong>Chiral ION-QN</strong></a><strong> &amp; </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1240"><strong>Chiral ION-QD</strong></a></h4><ul><li><strong>Consistent performance:</strong> Make your results easily transferable</li><li><strong>Remarkable selectivity:</strong> Easily separate closely eluting peaks</li><li><strong>Ideal for method development:</strong> Reduce your time and costs spent</li></ul><h4><strong>How can Galochrom columns enhance your methods?</strong></h4><ul><li><strong>Consistent reproducibility: </strong>Eliminate process variance and achieve your results with confidence</li><li><strong>Seamless method scale-up: </strong>Transform your analytical method into preparative purification</li><li><strong>Save time and money: </strong>Spend more time on efficient separations and less time on troubleshooting</li><li><strong>Better peak shapes: </strong>Easily resolve even the most challenging mixtures</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Method_Development_and_Preparative_Chiral_ION_QN_and_Chiral_ION_QD_columns_Consistent_reproducibility_Seamless_method_scale_up_Save_time_and_money_Better_peak_shapes_4b220cd029.jpg" alt="Galochrom: Consistent reproducibility, Seamless method scale-up, Save time and money, Better peak shapes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Method_Development_and_Preparative_Chiral_ION_QN_and_Chiral_ION_QD_columns_Consistent_reproducibility_Seamless_method_scale_up_Save_time_and_money_Better_peak_shapes_4b220cd029.jpg 245w," sizes="100vw" width="921" height="175"></p><h3><strong>Preparative columns</strong></h3><p><i>Obtain higher throughput while maintaining your product quality</i></p><h4><strong>Optimized loading parameters</strong></h4><p>Our preparative columns provide optimized properties for the purification of wide array of compounds. Some of their inherent parameters include:</p><ul><li>High surface area for increased loading</li><li>Smooth silica for stable packed beds</li><li>Optimal particle and pore size for outstanding performance</li><li>Exceptional quality of silica for column longevity</li></ul><h4><strong>Uniform media with intact particles</strong></h4><p>Our state-of-art packing process eliminates potential bed collapse and ensures longer column lifetime. The combination of optimal slurry amounts and packing<br>pressure significantly reduces column-to-column variability. As a result, each column contains uniform stationary phase with optimal packing density.</p><h4><strong>Technical information</strong></h4><ul><li><strong>Particle Size [μm]:</strong> 5</li><li><strong>Pore Size [Å]:</strong> 120</li><li><strong>Surface Area [m²/g]:</strong> 300</li><li><strong>pH Stability:</strong> 2-8</li><li><strong>Max. Pressure [bar]: </strong>200</li><li><strong>Max. Temperature:</strong> 50 °C</li><li><strong>Silica Morphology:</strong> Spherical, fully porous</li><li><strong>Endcapping:</strong> Yes</li></ul><p>Moreover, you can order custom column dimensions along with preferred particle size.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Method_Development_and_Preparative_Chiral_ION_QN_and_Chiral_ION_QD_columns_Preparative_Chiral_ION_QN_and_Chiral_ION_QD_columns_ac8fd1acff.jpg" alt="Galochrom: Preparative Chiral ION-QN &amp; Chiral ION-QD columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Method_Development_and_Preparative_Chiral_ION_QN_and_Chiral_ION_QD_columns_Preparative_Chiral_ION_QN_and_Chiral_ION_QD_columns_ac8fd1acff.jpg 245w," sizes="100vw" width="2026" height="1229"></p><h3><strong>Order information</strong></h3><h4><strong>Analytical Columns</strong></h4><h5><strong>Chiral ION-QN</strong></h5><ul><li><strong>Dimensions [mm]: 150 x 3</strong></li><li>Porosity: [Å] 200</li><li>Cat. No.: ION01-A150</li><li><strong>Price [€]: 1 300</strong></li></ul><h5><strong>Chiral ION-QD</strong></h5><ul><li><strong>Dimensions [mm]: 150 x 3</strong></li><li>Porosity: [Å] 200</li><li>Cat. No.: ION02-A150</li><li><strong>Price [€]: 1 300</strong></li></ul><p><span style="font-size:12px;">*As an alternative, we can also offer 150x3 mm, 3 μm with 120 Å</span></p><h4><strong>Preparative Columns</strong></h4><h5><strong>Chiral ION-QN</strong></h5><ul><li><strong>Dimensions [mm]: 150 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION03-P150</li><li><strong>Price [€]: 9 800</strong></li></ul></li><li><strong>Dimensions [mm]: 250 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION04-P250</li><li><strong>Price [€]: 11 300</strong></li></ul></li></ul><h5><strong>Chiral ION-QD</strong></h5><ul><li><strong>Dimensions [mm]: 150 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION05-P150</li><li><strong>Price [€]: 9 800</strong></li></ul></li><li><strong>Dimensions [mm]: 250 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION06-P250</li><li><strong>Price [€]: 11 300</strong></li></ul></li></ul><blockquote><h3><strong>SPECIAL OFFER 1+1</strong></h3><p><span style="font-size:22px;"><strong>Buy 1 preparative column and get analytical column for FREE</strong></span></p><p>You can choose 150x4 or 250x4 column with the same sorbent as your preparative column.</p></blockquote>

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