<ul>
<li>Price: 155 € + VAT</li>
<li>840 Pages</li>
<li>ISBN 978-80-11-04472-5</li>
<li>Language: English </li>
<li>Published in June 2024 by Czech Chromatographic School</li>
<li>Authors: Lucie Nováková, Michal Douša, Petr Česla, Jiří Urban, and collective</li>
<li>Reviewers: Davy Guillarme, Deirdre Cabooter</li>
</ul>
<h4>How to buy the book</h4>
To order the book, please fill in the <a href="https://docs.google.com/forms/d/e/1FAIpQLSfbb_6rwE2q-jLWjgIHbiWPAuDUK-6bjcZhFAPWK4TN1r_DFw/viewform">order form</a> and send it back to store@czechhplcbook.com. Based on this information, we will issue an invoice including shipping. Once the payment is received, we will ship the book to you.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Czech_Chromatographic_School_Launch_of_the_book_Modern_HPLC_separations_in_theory_and_practice_c03aa3bd7b.jpg" alt="Czech Chromatographic School: Launch of the book: Modern HPLC separations in theory and practice"/>
<h3>Book Content</h3>
<h5>1 HISTORY AND DEVELOPMENT OF LIQUID CHROMATOGRAPHY</h5>
<ul>
<li>1.1 FROM THE FIRST CHROMATOGRAPHIC SEPARATIONS TO HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY</li>
<li>1.2 DEVELOPMENT OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY IN THE NEW MILLENNIUM</li>
</ul>
<h5>2 BASIC CONCEPTS OF CHROMATOGRAPHIC SEPARATION</h5>
<ul>
<li>2.1 DESCRIPTION OF RETENTION IN THE CHROMATOGRAPHIC SYSTEM</li>
<li>2.2 DESCRIPTION OF SELECTIVITY IN THE CHROMATOGRAPHIC SYSTEM</li>
<li>2.3 DESCRIPTION OF EFFICIENCY IN THE CHROMATOGRAPHIC SYSTEM</li>
<li>2.4 DESCRIPTION OF RESOLUTION</li>
<li>2.5 SEPARATIONS USING GRADIENT ELUTION</li>
</ul>
<h5>3 HPLC INSTRUMENTATION</h5>
<ul>
<li>3.1 BASIC DESCRIPTION OF A LIQUID CHROMATOGRAPH</li>
<li>3.2 MOBILE PHASE TRANSPORT</li>
<li>3.3 SAMPLE INJECTION</li>
<li>3.4 CHROMATOGRAPHIC COLUMNS FOR HPLC</li>
<li>3.5 DETECTION IN HPLC</li>
<li>3.6 DERIVATIZATION IN HPLC</li>
</ul>
<h5>4 STATIONARY PHASES</h5>
<ul>
<li>4.1 PARAMETERS CHARACTERIZING STATIONARY PHASES</li>
<li>4.2 SILICA GEL</li>
<li>4.3 CHEMICALLY BONDED SILICA GEL BASED STATIONARY PHASES</li>
<li>4.4 METAL OXIDE-BASED STATIONARY PHASES</li>
<li>4.5 STATIONARY PHASES BASED ON ORGANIC POLYMERS</li>
<li>4.6 STATIONARY PHASES FOR ION-EXCHANGE CHROMATOGRAPHY</li>
<li>4.7 HYBRID STATIONARY PHASES</li>
<li>4.8 POROUS GRAPHITIC CARBON-BASED STATIONARY PHASES</li>
<li>4.9 MIXED-MODE STATIONARY PHASES</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Czech_Chromatographic_School_Launch_of_the_book_Modern_HPLC_separations_in_theory_and_practice_in_action_42a0240167.jpg" alt="Czech Chromatographic School: Launch of the book: Modern HPLC separations in theory and practice in action"/>
<h5>5 CHROMATOGRAPHIC MODES</h5>
<ul>
<li>5.1 NORMAL-PHASE CHROMATOGRAPHY (NP-HPLC)</li>
<li>5.2 REVERSED-PHASE CHROMATOGRAPHY (RP-HPLC)</li>
<li>5.3 ION-EXCHANGE CHROMATOGRAPHY (IEC)</li>
<li>5.4 HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY (HILIC)</li>
<li>5.5 HYDROPHOBIC INTERACTION CHROMATOGRAPHY (HIC)</li>
<li>5.6 MIXED-MODE CHROMATOGRAPHY</li>
<li>5.7 ION-PAIR CHROMATOGRAPHY</li>
<li>5.8 AFFINITY CHROMATOGRAPHY</li>
<li>5.9 SIZE-EXCLUSION CHROMATOGRAPHY (SEC)</li>
<li>5.10 CHIRAL CHROMATOGRAPHY</li>
</ul>
<h5>6 CURRENT TRENDS IN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY</h5>
<ul>
<li>6.1 ULTRA-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (UHPLC)</li>
<li>6.2 POROUS SHELL PARTICLES</li>
<li>6.3 MONOLITHIC COLUMNS</li>
<li>6.4 HIGH-TEMPERATURE LIQUID CHROMATOGRAPHY (HTLC)</li>
<li>6.5 SUPERCRITICAL FLUID CHROMATOGRAPHY</li>
<li>6.6 TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY</li>
<li>6.7 MINIATURIZATION IN LIQUID CHROMATOGRAPHY</li>
</ul>
<h5>7 MODERN HPLC PRACTICE</h5>
<ul>
<li>7.1 MOBILE PHASE PREPARATION AND USE</li>
<li>7.2 CHROMATOGRAPHIC COLUMN – CARE AND USE</li>
<li>7.3 REGULAR MAINTENANCE OF HPLC SYSTEM</li>
</ul>
<h5>8 HPLC METHOD DEVELOPMENT AND OPTIMIZATION</h5>
<ul>
<li>8.1 PHYSICOCHEMICAL PROPERTIES OF THE ANALYTES AND METHOD OBJECTIVE</li>
<li>8.2 SELECTION OF DETECTION APPROACH</li>
<li>8.3 SELECTION OF CHROMATOGRAPHIC MODE</li>
<li>8.4 SELECTION OF STATIONARY AND MOBILE PHASES</li>
<li>8.5 PRELIMINARY EXPERIMENTS</li>
<li>8.6 METHOD OPTIMIZATION</li>
<li>8.7 SOLVING SEPARATION PROBLEMS IN METHOD DEVELOPMENT</li>
<li>8.8 SPECIFIC OPTIMIZATION APPROACHES</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Czech_Chromatographic_School_Launch_of_the_book_Modern_HPLC_separations_in_theory_and_practice_Authors_Lucie_Novakova_Michal_Dousa_Petr_Cesla_Jiri_Urban_and_collective_fcd013e2d2.jpg" alt="Czech Chromatographic School: Launch of the book: Modern HPLC separations in theory and practice, Authors (Lucie Nováková, Michal Douša, Petr Česla, Jiří Urban, and collective)"/>
<h5>9 SAMPLE PREPARATION FOR CHROMATOGRAPHIC ANALYSIS</h5>
<ul>
<li>9.1 CONVENTIONAL TECHNIQUES FOR SAMPLE PREPARATION</li>
<li>9.2 MODERN TRENDS IN SAMPLE PREPARATION TECHNIQUES</li>
</ul>
<h5>10 PREPARATIVE CHROMATOGRAPHY</h5>
<ul>
<li>10.1 PREPARATIVE HPLC</li>
<li>10.2 PREPARATIVE SFC</li>
</ul>
<h5>11 DATA ANALYSIS IN HPLC</h5>
<ul>
<li>11.1 QUALITATIVE ANALYSIS</li>
<li>11.2 QUANTITATIVE ANALYSIS</li>
<li>11.3 METABOLOMICS APPROACHES</li>
</ul>
<h5>12 METHOD VALIDATION IN HPLC</h5>
<ul>
<li>12.1 DEFINITIONS OF METROLOGICAL TERMS</li>
<li>12.2 VALIDATION PARAMETERS</li>
<li>12.3 PRACTICAL IMPLEMENTATION OF VALIDATION</li>
<li>12.4 PHARMACOPEIAL METHODS</li>
<li>12.5 SYSTEM SUITABILITY TEST</li>
<li>12.6 HPLC SYSTEM QUALIFICATION</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Czech_Chromatographic_School_Launch_of_the_book_Modern_HPLC_separations_in_theory_and_practice_Author_Lucie_Novakova_and_Reviewers_Davy_Guillarme_Deirdre_Cabooter_4aa4acab35.jpg" alt="Czech Chromatographic School: Launch of the book: Modern HPLC separations in theory and practice, Author (Lucie Nováková) and Reviewers: Davy Guillarme, Deirdre Cabooter"/>
<h5>13 HPLC TROUBLESHOOTING</h5>
<ul>
<li>13.1 BACK PRESSURE ISSUES</li>
<li>13.2 BASELINE ISSUES</li>
<li>13.3 RETENTION TIME ISSUES</li>
<li>13.4 PEAK SHAPE AND PEAK AREA PROBLEMS</li>
<li>13.5 CHROMATOGRAM PROBLEMS</li>
</ul>
<h5>14 LITERATURE REFERENCES</h5>
<h5>15 TABLES USEFUL IN HPLC DAILY PRACTICE</h5>
<ul>
<li>15.1 DYNAMIC VISCOSITY η OF ACETONITRILE/WATER AND METHANOL/WATER MIXTURES</li>
<li>15.2 MISCIBILITY OF ORGANIC SOLVENTS USED IN HPLC</li>
<li>15.3 ABSORPTION MAXIMA AND MOLAR EXTINCTION COEFFICIENTS</li>
</ul>
<h5>16 LIST OF ABBREVIATIONS</h5>
<h5>17 LIST OF SYMBOLS</h5>
<h5>CHROMATOGRAPHIC VENDORS’ PRESENTATION</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Czech_Chromatographic_School_Modern_HPLC_separations_in_theory_and_practice_Book_Partners_97d8ad93eb.jpg" alt="Czech Chromatographic School: Modern HPLC separations in theory and practice - Book Partners"/>
<h3>Authors’ short biographies</h3>
Lucie Nováková is a Full Professor at the Charles University, Faculty of Pharmacy in Hradec Králové, Department of Analytical Chemistry, the Czech Republic. Her research is focused on separation techniques, in liquid and supercritical phase, and their coupling to mass spectrometry. She is involved in a broad scope of research projects focused on pharmaceutical analysis, doping control, plant analysis, and bioanalytical methods. She extended her scientific experience during the fellowships at world-recognized universities, such as the University of Geneva and Vrije Universiteit Brussel, beyond others. She published over 155 peer-reviewed scientific articles with H-index of 41 and more than 5500 citations, one book, and nine book chapters. She is also widely involved in teaching and education activities, such as HPLC and SFC training courses, seminars, but also conference lectures and organization. Recently, she has become also a Vice-dean for External and International Relationships.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Czech_Chromatographic_School_Lucie_Novakova_Charles_University_and_Michal_Dousa_Zentiva_195c22df26.jpg" alt="Czech Chromatographic School: Lucie Nováková (Charles University) and Michal Douša (Zentiva)"/>
Michal Douša has been working in the pharmaceutical company Zentiva since 2007 as a head of the separation methods department. His research is focused on separation techniques, especially hydrophilic interaction liquid chromatography and chiral separation. He gained previous work experience at various workplaces in the field of HPLC analysis of water, soil, food, and feed. He is the author of a book on HPLC theory and practice and one book chapter. He published over 65 peer-reviewed scientific articles withmore than 850 citations and H-index of 18. He is also involved in teaching and education activities, such as HPLC and validation training courses and seminars.
Petr Česla is an Associate professor of analytical chemistry, Head of the Department of analytical chemistry, and Vice-dean for Research and Creative Activities of the Faculty of Chemical Technology, University of Pardubice. In his research work, he focuses on the development of separation techniques in liquid phase, mainly liquid chromatography and capillary electrophoresis, with the special attention to the two-dimensional separations, optimization procedures, data processing, and coupling of LC and CE to mass spectrometry. He has been engaged in many research projects and acts as principal investigator of several projects of Czech Science Foundation. He is author of more than 60 papers in scientific journals with H-index of 18 and more than 1070 citations, co-author of one book and two book chapters.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Czech_Chromatographic_School_Petr_Cesla_University_of_Pardubice_and_Jiri_Urban_Masaryk_University_aa8af25d14.jpg" alt="Czech Chromatographic School: Petr Česla (University of Pardubice) and Jiří Urban (Masaryk University)"/>
Jiří Urban received a Ph.D. in 2007 in the group of Pavel Jandera at the University of Pardubice, the Czech Republic, where he worked until 2016. During 2009 – 2011, he followed post-doctoral research at the University of California, Berkeley, USA, in the František Švec and Jean M. J. Fréchet group. In 2017 he moved to the Department of Chemistry, Masaryk University, Brno, Czech Republic, where he became an Associate Professor in 2018. In his research, he utilizes multifunctional polymer monoliths to develop new analytical methods applicable to metabolomics and proteomics. He also focuses on the design of new instrumental setups for two-dimensional liquid chromatography. He published over 51 peer-reviewed scientific articles with more than 1400 citations and H-index of 22.
<h3>Reviewers’ feedback</h3>
“It was an honor to serve as a reviewer for this comprehensive book on liquid chromatography, and I am grateful to the authors for the opportunity to contribute to such a valuable resource. The book provides an excellent balance of content for those new to the field and those with advanced expertise, making it a versatile and accessible guide for chromato- graphers at all levels. The clarity of the explanations, the quality of the figures (I cannot imagine the time it takes to draw all the figures contained within the book), coupled with the in-depth discussions, allow me to further expand my understanding of liquid chromatography in some points. This book, with its well-structured content and broad appeal, is sure to become a bestseller and make a significant contribution to the chromatography community. I am confident that readers, whether novice or experienced, will find immense value in its pages.”
Davy Guillarme
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Czech_Chromatographic_School_Davy_Guillarme_University_of_Geneva_and_Deirdre_Cabooter_KU_Leuven_4e43fe9111.jpg" alt="Czech Chromatographic School: Davy Guillarme (University of Geneva) and Deirdre Cabooter (KU Leuven)"/>
 “This book presents a very comprehensive overview of modern HPLC theory and practice that will be of great interest to anyone looking to immerse themselves into the technique. The book gives an overview of all aspects of liquid chromatography, from basic concepts of chromatography, covering all chromatographic modes, to novel trends in HPLC, such as 2D-LC and micro-pillar array columns, and a very useful chapter on troubleshooting. The many clear and informative illustrations make the book very practical and applicable. This is a must-have for anyone who is teaching or learning HPLC.”
Deirdre Cabooter

Launch of the book: Modern HPLC separations in theory and practice

<h3><a target="_blank" rel="noopener noreferrer" href="https://www.isc2026.org/">ISC 2026</a></h3>The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/314">International Symposium on Chromatography (ISC)</a> represents the oldest conference series on separation science. ISC symposia have been organised since 1956 in each even year. ISC is one of the premier meetings series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.The major focus of the symposium will be on the impact of chromatography and separation science to meet the needs of the pharmaceutical, environmental, food and health industry, as well as science and medicine. The symposium programme will reflect these themes and highlight new challenges and emerging opportunities for the science and marketing of separation and detection systems and methods.As an integral part of the scientific programme of lectures and poster sessions, an international exhibition and vendor seminars on instrumentation and services for chromatography, separation science and mass spectrometry will be organised. ISC 2026 will provide the perfect forum for attendees from academia, industry and government research institutions for scientific exchange and networking.<h3>ISC 2026 SYMPOSIUM KEY DATES</h3><h4>6-10 SEPTEMBER 2026</h4><ul><li>15 January 2026: Opening of Early-bird Registration &amp; Abstract submission</li><li>11 March 2026: Deadline for abstract submission (orals)</li><li>21 April 2026: Sending acceptance letters (orals)</li><li>15 May 2026: Deadline of early-bird registration and payment &amp; Author registration deadline (orals)</li><li>1 June 2026: Preliminary detailed program on web</li><li>1 June 2026: Deadline for abstract submission (posters)</li><li>16 June 2026: Sending acceptance letters (posters)<ul><li>Authors who submit their abstracts with a poster preference by 5 May 2026 will be notified of the outcome by 12 May 2026 and will have the opportunity to register at the early-bird rate until 15 May 2026.</li></ul></li><li>30 June 2026: Author registration deadline (posters)</li><li>30 July 2026: Deadline for abstract submission (late-breaking posters)<ul><li>without the possibility of competing for the Best Poster Award</li></ul></li><li>11 August 2026: Sending acceptance letters (late-breaking posters)</li><li>18 August 2026: Author registration deadline (late-breaking posters)</li><li>25 August 2026: Deadline for late registration &amp; payment&nbsp;</li><li>6-10 September 2026: Symposium</li></ul><h3>REGISTRATION</h3><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://www.czech-in.org/cmPortalV15/Account/Login?ReturnUrl=%2FcmPortalV15%2FPortal%2FISC26%2Fnormal">Register HERE for the ISC 2026</a></blockquote><h3>ABSTRACT SUBMISSION</h3><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://www.czech-in.org/cmPortalV15/Account/Login?ReturnUrl=%2FcmPortalV15%2FPortal%2FISC26%2Fnormal">Submit your Abstract HERE</a></blockquote><h3>SCIENTIFIC PROGRAM</h3><h4>SHORT COURSES</h4><h5>SC 1 Modern HPLC Separation in Theory and Practice</h5>🗓️ SATURDAY, 5 SEPTEMBER 2026🕗 9:30 – 17:00High-performance liquid chromatography (HPLC) is one of the most powerful and widely applied separation techniques in modern analytical chemistry. This short course provides a comprehensive introduction to contemporary HPLC, combining fundamental chromatographic theory with practical guidance relevant to everyday analytical practice.In the first part, we will focus on the overview of key separation principles, including compound retention, selectivity, separation efficiency, and gradient elution. We will cover HPLC instrumentation, column technologies, and stationary phase chemistry, with emphasis on how these factors influence separation efficiency and selectivity, but also method robustness.Subsequently, the course introduces different separation modes and recent developments in liquid chromatography, including ultra-high-performance liquid chromatography (UHPLC), high-temperature LC, supercritical fluid chromatography (SFC), and two-dimensional liquid chromatography (2D-LC). Practical strategies for method development and optimization are emphasized throughout the course, enabling participants to apply modern HPLC solutions effectively in their own work.Participants will gain a solid understanding of HPLC theory, practical method development, and an overview of current trends in UHPLC, SFC, and multidimensional LC. The course concludes with an interactive knowledge quiz, offering participants an opportunity to test their understanding and compete for a copy of the book <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1210">Modern HPLC Separations in Theory and Practice</a>, authored by the course instructors. The course also offers a unique opportunity to meet all the book authors in person and to have your own book copy signed.<h5>Lecturers</h5><ul><li>Lucie Nováková<ul><li>Lucie Nováková is a Full Professor in Analytical Chemistry at the Charles University, Faculty of Pharmacy in Hradec Králové, Czech Republic. Her research is focused on separation techniques, including UHPLC, SFC and their coupling to mass spectrometry. She is involved in a wide scope of research projects in pharmaceutical analysis, doping control, plant analysis, and bioanalytical methods. She authored the <a target="_blank" rel="noopener noreferrer" href="https://czechhplcbook.com">book on HPLC theory and practice in Czech and in English</a> and ten book chapters. She published almost 180 peer-reviewed scientific articles with more than 6600 citations and an h-index of 44. She is also widely involved in teaching and education activities.</li></ul></li><li>Jiří Urban<ul><li>Jiri Urban received his Ph.D. in 2007 under the supervision of Pavel Jandera at the University of Pardubice, Czech Republic, where he remained until 2016. From 2009 to 2011, he conducted postdoctoral research at the University of California, Berkeley, USA, in the groups of Frantisek Svec and Jean M. J. Fréchet. In 2017, he joined the Department of Chemistry at Masaryk University in Brno, Czech Republic, where he was appointed Associate Professor in 2018 and subsequently became a Full Professor in 2025. His research focuses on the use of multifunctional polymer monoliths for developing new analytical methods applicable to metabolomics and proteomics. He also investigates retention modeling in chromatographic method development and the design of novel instrumental setups for two-dimensional liquid chromatography.&nbsp;</li></ul></li><li>Petr Česla<ul><li>Petr Česla is an Associate Professor and Head of the Department of Analytical Chemistry at the Faculty of Chemical Technology, University of Pardubice, Czech Republic. His research focuses on the development of liquid chromatographic separations, including two-dimensional separations, retention modeling, and optimization procedures. The main application areas of his work include the analysis of naturally occurring plant metabolites, pharmaceuticals, and industrial samples such as surfactants, oligomers and synthetic dyes. He also represents the Czech Republic in the Central European Group of Separation Sciences. &nbsp;</li></ul></li><li>Michal Douša<ul><li>Michal Douša has served as the Head of the Separation Methods Department at the pharmaceutical company Zentiva since 2007. His research focuses on separation techniques, particularly HILIC, chiral separation, and derivatization techniques. He has prior experience in HPLC analysis of soil, food, and feed. He is the author of a book on HPLC theory and practice and one book chapter. He has published over 67 articles, accumulating more than 1039 citations (h-index 20). He is also actively involved in teaching and educational activities, including HPLC and validation training courses. He serves as the Chairman of the Czech Chromatography School association.&nbsp;</li></ul></li></ul>

ISC 2026 Short Course 1 - Modern HPLC Separation in Theory and Practice

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The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

The Czech Chromatographic School is a non-profit private scientific association. Its goal is to bring together experts and beginners in the field of separation science from industry, academia, and vendors to share their knowledge.

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