HPLC columns are a critical component of chromatographic systems for the effective separation and analysis of components across a wide range of applications. Each HPLC column has a limited lifespan, and to maximize its utility, proper care is essential, which can extend its usage time. It is recommended to regularly monitor the column's efficiency and separation capabilities using standards. If you plan to store the column for an extended period, use a mobile phase that inhibits microbial growth (such as mobile phases containing sodium azide or higher concentrations of methanol, acetonitrile, etc.).To achieve optimal HPLC column performance and longevity, follow these recommendations:<ul><li>#1 Check the column‘s efficiency on a regular basis.</li><li>#2 Always sure that both mobile phases are well miscible when changing them.</li><li>#3 Avoid salts from precipitation in the column, e.g. high content of the organic component in the buffer.</li><li>#4 Use only perfectly filtered and degassed mobile phases.</li><li>#5 Keep the column from drying out. Close securely when not in use.</li><li>#6 For long-term preservation, use a mobile phase to inhibit the growth of mold and bacteria, for example, by adding NaN3.</li><li>#7 Use the column according in direction on the label. Reverse flow of 5 - 10 column volumes can assist in clearing a clogged inlet frit when there is a significant column backpressure.</li><li>#8 Use guard column to protect your analytical column from particulates, solvent impurities and retained sample components.</li></ul>The right column choice is as essential for successful analysis as is its subsequent care. Watrex Praha offers a broad portfolio of HPLC columns, which they prepare in-house at their laboratory.<h3>Types filling of and Applications for HPLC Columns</h3><h4>1. Reversed Phase</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=deltasil+100+c18&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex reversed-phase columns</a> use high-quality silica-based stationary phases modified with C18 alkyl chains, with effective endcapping to deactivate residual silanol groups.They are ideal for the separation of slightly polar to nonpolar compounds across a wide range of products, including components of medicines, food products and supplements, environmental pollutants or other natural products.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Figure_1_Chromatogram_1_Column_Watrex_Ion_Plus_TM_CS_19_200x4mm_36d35a09a4.jpg" alt="Watrex: Figure 1: Chromatogram 1 - Column: Watrex IonPlus CS19, 200x4mm; Mobile phase: 4mM HNO3; Flowrate: 1.0 ml/min; Detection: Conductivity without suppression. Chromatogram 2 - Column: Watrex IC Anion I; Mobile phase: 4mM Na 2 CO 3 + 1mM NaHCO 3 + 0.1mM NaSCN; Flowrate: 1,2 ml/min; Pressure: 54 bar; Detection: Conductivity with suppression; Injection: 20 μl." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Figure_1_Chromatogram_1_Column_Watrex_Ion_Plus_TM_CS_19_200x4mm_36d35a09a4.jpg 245w," sizes="100vw" width="2419" height="1181"><ul><li>Column Dimensions: Depending on customer requirements – from standard analytical columns (2.1 - 4.6 mm diameter, typical lenght 100 mm to 250 mm) packed with strictly monodisperse particles sized 3 or 5 μm. Preparative columns suitable for the separation and purification of biomolecules (peptides, amino acids, nucleotides, etc.) with larger porosity are available in semi-preparative (8 mm diameter) and preparative (20 mm diameter) formats.</li><li>Example Applications: Analysis of pharmaceuticals, environmental analyses, food composition.</li></ul><h4>2. Ion-Exchange Columns</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=ionplus&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex ion-exchange columns</a> are designed for the separation of ionic compounds, based on interactions between ions in the stationary phase and analytes. These columns are suitable for the separation of inorganic ions, amino acids, or proteins.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Figure_2_Chromatogram_3_Column_Watrex_Deltasil_100_C18_258df30fcd.jpg" alt="Watrex: Figure 2: Chromatogram 3 - Column: Watrex Deltasil 100 C18; Mobile phase: 85% ACN; Flowrate:0.65 ml/min; Pressure: 54 bar; Detection: 254 nm; Injection: 2 μl." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Figure_2_Chromatogram_3_Column_Watrex_Deltasil_100_C18_258df30fcd.jpg 245w," sizes="100vw" width="2419" height="1181"><ul><li>Column Dimensions: Columns available in various dimensions.</li><li>Example Applications: Analysis of ions, biomolecules, peptide separation.</li></ul><h4>3. Ion-Exclusion Columns</h4>These <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=polymer+iex&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex Polymer IEX HPLC columns</a> are suitable for the separation of weak organic acids and carbohydrates, based on ion exclusion principles. The basis is polymer monodisperse particles with a size of 8 μm modified by sulfonation and supplied in various ionic forms.<ul><li>The H-form is intended for the determination of weak organic acids in food or agricultural and veterinary products.</li><li>The Ca- and Pb-forms are typically used for the determination of mono-, di- and oligosaccharides in the food industry.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Figure_3_Chromatogram_4_Column_Watrex_Polymer_IEX_Ca_250x8mm_Chromatogram_5_Column_Watrex_Polymer_IEX_H_c3818e4cc1.jpg" alt="Watrex: Figure 3: Chromatogram 4 - Column: Watrex Polymer IEX Ca 250x8mm; Eluent: H2O; Flow-rate: 0.5 ml/min; Pressure: 26 bar; Inj. Volume: 20 μl; Concentration: 20 ppm; Temperature: 90 °C; Detection: RI. Chromatogram 5 - Column: Watrex Polymer IEX H 250x8mm; Eluent: 9mM H2SO4; Flow-rate: 0.5 ml/min; Pressure: 45 bar; Inj. Volume: 20 μl; Concentration: 20 ppm; Temperature: ambient; Detection: UV 210 nm." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Figure_3_Chromatogram_4_Column_Watrex_Polymer_IEX_Ca_250x8mm_Chromatogram_5_Column_Watrex_Polymer_IEX_H_c3818e4cc1.jpg 245w," sizes="100vw" width="2419" height="1181"><ul><li>Column Dimensions: 8 mm diameter and lengths of 250 or 300 mm.</li><li>Example Applications: Analysis of organic acids in food products, fermentation processes.</li></ul><h4> 4. Gel Permeation/Size Exclusion Chromatography (GPC/SEC) Columns</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=deltagel&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex gel permeation chromatography (GPC) columns</a> are used for separating highmolecular-weight compounds, typically synthetic or water-soluble polymers, based on molecule size. Smaller molecules penetrate more deeply into the porous structure of the sorbents and thus elute later than larger molecules.According to the type of stationary phase, the columns are usually divided into Gel Permeation Chromatography (GPC) columns used in the separation of synthetic polymers with organic mobile phases (e.g. tetrahydrofuran, THF, DMF, toluene THF) on polystyrene - divinylbenzene sorbents with defined porosity.In contrast, the term Size-Exclusion Chromatography (SEC) typically refers to columns for the separation of water-soluble polymers, biomacromolecules (e.g. peptides or proteins), which occur in buffered aqueous phases on porous hydrophilic sorbents (e.g. based on agarose or methacrylate particles modified with hydrophilic groups).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Figure_4_Chromatogram_6_Delta_Gel_TM_Mixed_C_Chromatogram_7_Delta_Gel_TM_Mixed_D_541ef2ed25.jpg" alt="Watrex: Figure 4: Chromatogram 6 - DeltaGelTM Mixed C. Chromatogram 7 - DeltaGelTM Mixed D." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Figure_4_Chromatogram_6_Delta_Gel_TM_Mixed_C_Chromatogram_7_Delta_Gel_TM_Mixed_D_541ef2ed25.jpg 245w," sizes="100vw" width="2419" height="946"><ul><li>Column dimensions: Watrex GPC/SEC columns are available in analytical (8x250 mm or 8x300 mm) and preparative configurations (20x300 mm).</li><li>Examples of use: Separation of synthetic polymers, peptides, proteins, polysaccharides.</li></ul><h3>Advantages of Watrex HPLC columns</h3>Columns manufactured directly in the Watrex laboratory provide several fundamental advantages:<ul><li>High quality at friendly prices. Thanks to the fact that they produce the columns themselves, they can offer top quality at very affordable prices.</li><li>A wide range of sizes and types. It offers columns adapted to various chromatographic techniques, including analytical and preparative applications.</li><li>Flexibility in applications. Watrex columns are compatible with a variety of mobile phases and can be used for the separation of ionic compounds, organic acids, polymers and biomolecules.</li></ul><h3>Conclusion</h3><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=48&amp;distributorItems=59&amp;manufacturerItems=93">Watrex HPLC and GPC/SEC columns </a>are a highly reliable solution for a wide range of chromatographic applications and combine high quality with a favorable price. Whether you need columns for reverse phase, ion exchange, ion exclusion, size exclusion chromatography (SEC) or gel permeation chromatography (GPC), Watrex products guarantee you excellent separation properties and reproducible results.For more information, feel free to contact Watrex <a href="mailto:watrex@watrex.com">watrex@watrex.com</a>) or visit their website (<a target="_blank" rel="noopener noreferrer" href="https://watrex.com/cs/uvod/?utm_source=labrulez">www.watrex.com</a>).

8 points on how to care for HPLC columns

High-Performance Liquid Chromatography (HPLC) is one of the most essential tools in modern analytical chemistry. Although it plays a vital role in pharmaceutical research, food testing, and environmental analysis, its ecological footprint is far from negligible. The consumption of organic solvents, waste generation, and energy demands are key concerns for today’s laboratories.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_green_uvod_1cb98c92c9.jpg" alt="Watrex Prague: Introductory illustrative image" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_green_uvod_1cb98c92c9.jpg 245w," sizes="100vw" width="1600" height="900">This has led to the rise of “green chromatography”&nbsp;– the effort to make HPLC processes more sustainable without compromising analytical performance or data quality.&nbsp;<h4>1. Smaller Volumes, Greater Efficiency&nbsp;</h4>The foundation of eco-friendly chromatography lies in reducing solvent consumption. Modern HPLC systems support column miniaturization&nbsp;and operation with lower mobile-phase flow rates. By adopting UHPLC technology, laboratories can achieve the same separation efficiency in shorter analysis times while using less solvent. The result is not only a smaller environmental footprint but also significant cost savings.&nbsp;<h4>2. Solvent Recycling – The Key to Sustainability&nbsp;</h4>One of the most effective ways to reduce HPLC’s environmental impact is solvent recycling. Most of the mobile phase that exits the column remains clean and reusable, even after the separation process. This is where intelligent recycling systems come into play:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/dedicated-instruments/ufo-solvent-recycler-peek-version/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">UFO Solvent Recycler&nbsp;</a>– an advanced system that detects when a chromatographic peak ends and automatically redirects the flow so that pure solvent is recovered back into the mobile-phase reservoir. This approach can lead to up to 80% solvent savings&nbsp;while maintaining full analytical integrity.&nbsp;</li><li><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/dedicated-instruments/spr-200-solvent-recycler-peek-valve/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">SPR-200 Solvent Recycler</a>&nbsp;– a robust solution designed for long-term, routine analyses. It enables continuous solvent recovery&nbsp;and minimizes hazardous waste generation. Its reliability and easy integration make it a popular choice for pharmaceutical and academic laboratories alike.&nbsp;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_green_eco_342873e99d.jpg" alt="Watrex Fig. 2: UFO Solvent Saver with SPR-200 - an effective solution for solvent recycling." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_green_eco_342873e99d.jpg 245w," sizes="100vw" width="1600" height="900">Using these systems brings a double benefit&nbsp;– ecological and economic. Less waste means lower disposal costs, reduced solvent purchases, and improved laboratory safety.<h4>3. Monitoring Liquid Leaks and Preventing Waste&nbsp;</h4>Another essential aspect of green chromatography is liquid management and leak prevention. Every leak, overflow, or contamination event not only wastes solvent but also poses safety risks. The <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/dedicated-instruments/lss-205-starter-kit/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">LSS-205 Liquid Sensing System</a>&nbsp;offers a smart solution by detecting the presence of liquids at critical points of the HPLC setup. It identifies leaks, overflows, or empty bottles and immediately alerts the operator.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_green_eco_2_b4816cbe41.jpg" alt="Watrex Fig. 3: LSS-205 Liquid Sensing System is a smart solution that monitors the presence of liquids at critical points of the HPLC system.&nbsp;" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_green_eco_2_b4816cbe41.jpg 245w," sizes="100vw" width="1600" height="900">This not only protects the instrument and samples but also prevents unnecessary solvent waste and contamination&nbsp;— a small step with a significant environmental impact&nbsp;<h4>4. Method Optimization and “Green” Solvents&nbsp;</h4>Beyond hardware, method optimization&nbsp;plays a vital role in sustainability. Switching to less toxic solvents&nbsp;(e.g., ethanol instead of acetonitrile), shortening analysis times, and optimizing gradient programs&nbsp;can substantially reduce environmental impact without sacrificing analytical performance.&nbsp;<h4>5. Summary: Sustainability as a Standard, Not an Exception&nbsp;</h4>“Green chromatography” is not just a trend — it’s a necessary evolution toward more responsible laboratory practices. By integrating intelligent systems such as the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/dedicated-instruments/ufo-solvent-recycler-peek-version/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">UFO Solvent Recycler</a>, <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/dedicated-instruments/spr-200-solvent-recycler-peek-valve/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">SPR-200</a>, and<a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/dedicated-instruments/lss-205-starter-kit/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky"> LSS-205 Liquid Sensing System</a>, laboratories can significantly reduce solvent consumption, waste production, and overall energy use.&nbsp;The result?&nbsp;<ul><li>Lower operating costs&nbsp;</li><li>Increased safety&nbsp;</li><li>Smaller ecological footprint&nbsp;</li></ul><h4>Conclusion&nbsp;</h4>Eco-friendly chromatography is not about choosing between performance and sustainability - it’s about combining both through intelligent design. With solutions like the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/dedicated-instruments/lss-205-starter-kit/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">LSS-205</a>, <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/dedicated-instruments/ufo-solvent-recycler-peek-version/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">UFO Solvent Recycle</a>r, and <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/dedicated-instruments/spr-200-solvent-recycler-peek-valve/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">SPR-200</a>,&nbsp;laboratories can analyze efficiently while thinking and acting green.&nbsp;<blockquote>Are you more interested in the LSS-205, UFO or SPR-200 devices? Visit our website <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">www.watrex.com</a>&nbsp;or contact us at watrex@watrex.com.</blockquote>

Green Chromatography: How to Make HPLC More Eco- Friendly

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WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

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