Clarity Chromatography Software has a new addition. The new driver for <a href="https://gcms.labrulez.com/library?mainauthorItems=1&amp;search=2030&amp;publicationTypeItems=1-8-2">Shimadzu Nexis GC-2030</a> is part of the large family of directly controlled instruments.
Users can newly control all GC-2030 parameters directly from the software. You will find all details on its <a href="https://www.dataapex.com/product.php?id=controls-gc-shimadzu-nexis-gc-2030.php&amp;promotion=MKT1625">product&#39;s web page</a>. You can also download a <a href="https://www.dataapex.com/product/clarity-shimadzu-edition-demo?language_content_entity=en&amp;promotion=MKT1625">Clarity Shimadzu Edition demo version</a> and preview Clarity functionality. Clarity Shimadzu Edition is available only in Europe.
<blockquote>
<h5><a href="https://www.dataapex.com/product/clarity-shimadzu-edition-demo?language_content_entity=en&amp;promotion=MKT1625">Clarity - Shimadzu Edition DEMO version</a></h5>
</blockquote>
<blockquote>
<ul>
<li>Preview all Clarity - Shimadzu Edition functions</li>
<li>Experience the look and feel of working with Clarity - Shimadzu Edition</li>
<li>Data acquisition simulated from a data file</li>
<li>Demo data for data processing</li>
<li>All controlled instruments available in demo mode</li>
</ul>
</blockquote>
<a href="https://gcms.labrulez.com/library?mainauthorItems=95&amp;search=Clarity">DataApex Clarity</a> is a general-purpose chromatography software. Clarity can control <a href="https://www.dataapex.com/product.php?id=clarity-demo.php&amp;promotion=MKT1625">900+ different instruments</a> (including Agilent, Hitachi, PerkinElmer, etc.) from one environment.
It is a solution for the latest instrumentation and also for legacy instruments that can be connected via A/D converters. Software is modular; users can expand software by optional SW extensions that support various applications such as PDA/MS/GPC/SST etc. Clarity is multilingual; users can switch between English, Chinese, Russian, Spanish, French, and German.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Chromatograficky_software_Clarity_pro_prime_rizeni_plynoveho_chromatografu_Shimadzu_Nexis_GC_2030_2_b409d0ab46/DataApex-Chromatograficky-software-Clarity-pro-prime-rizeni-plynoveho-chromatografu-Shimadzu-Nexis-GC-2030-2.png" alt="DataApex: Clarity Chromatography Software for direct control of the Shimadzu&#39;s premier gas chromatograph Nexis GC-2030"/>

Clarity newly controls Nexis, Shimadzu's premier gas chromatograph GC-2030

DataApex is pleased to announce that a new control module for the <a href="https://lcms.labrulez.com/library?from=2020&amp;instrumentationItems=27&amp;mainauthorItems=1&amp;search=Nexera&amp;to=2022">Nexera LC-40 HPLC system</a> has been released in June 2022! It is a part of <a href="https://www.dataapex.com/product/controls-lc-shimadzu-nexera?language_content_entity=en">Clarity - Shimadzu Edition Chromatography Software</a> that is dedicated to the European market. Customers can use a control module for the Nexera LC-40 HPLC system in combination with hundreds of instruments supported in Clarity, including the <a href="https://gcms.labrulez.com/library?instrumentationItems=1&amp;mainauthorItems=1&amp;search=nexis+2030">Shimadzu Nexis GC-2030</a>.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/driven_by_clarity_1024_1_5c9d9d86ac/driven-by-clarity-1024-1.webp" alt="DataApex: Shimadzu Nexera LC-40 HPLC and Nexis GC-2030 systems controlled by Clarity Chromatography Software"/>
<a href="https://gcms.labrulez.com/library?mainauthorItems=95&amp;search=clarity">Clarity Chromatography Software</a> has a strong position in the chromatography data systems market. Clarity is highly regarded for its intuitive approach, excellent performance, cost-effectiveness, and proficient technical support. Clarity can control <a href="https://www.dataapex.com/controls">900+ different instruments</a> from one environment. Its wide range of data acquisition interfaces allows connection to virtually any chromatograph. Clarity supports various applications (PDA, MS, GPC, NGA, etc.).
<blockquote>
<h5><a href="https://www.dataapex.com/product/clarity-shimadzu-edition-demo?language_content_entity=en&amp;promotion=MKT1625">Clarity - Shimadzu Edition DEMO version</a></h5>
</blockquote>
<blockquote>
<ul>
<li>Preview all Clarity - Shimadzu Edition functions</li>
<li>Experience the look and feel of working with Clarity - Shimadzu Edition</li>
<li>Data acquisition simulated from a data file</li>
<li>Demo data for data processing</li>
<li>All controlled instruments available in demo mode</li>
</ul>
</blockquote>
DataApex is solely focused on chromatography software development. A strong emphasis is placed on technological innovation, visionary adoption of new laboratory standards, best practices, and extensive customer support. DataApex products are sold in over 100 countries around the world. Eleven chromatography instrument manufacturers resell privately labeled versions (OEMs) of DataApex’s software.
<blockquote>
To obtain further information about DataApex Clarity products, please contact us at sales@dataapex.com or visit <a href="https://www.dataapex.com/">www.dataapex.com</a>
</blockquote>

Shimadzu Nexera LC-40 HPLC system supported in Clarity

The Clarity MS Extension is a tool that is used for processing data that has been acquired from selected Mass Spectrometry detectors. Spectral data, together with chromatograms, add a third dimension to analytical data analysis.
<ul>
<li>👌 Combination of MS signals with conventional GC/HPLC detectors
</li>
<li>🔝 MS detectors can be used in combination with 1000+ instruments supported in Clarity
</li>
<li>💡 MS manufacturers can develop a driver for their own detectors using a Clarity Software Development Kit (SDK)
</li>
</ul>
The MS Extension expands the capability of Clarity and Clarity Offline Chromatography software by providing interactive spectral analysis, peak purity analysis, and compound identification that is based on a spectral library search. The extension is prepared to function with single quadrupole MS detectors and TOF MS detectors.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_software_Mass_Spectrometry_Extension_1_7fb5562dc3/DataApex-Clarity-software-Mass-Spectrometry-Extension-1.webp" alt="DataApex: Clarity software - Mass Spectrometry Extension"/>
All data is saved in a single file; a chromatogram at any m/z value or even only a spectrum can simply be recalled for review after an analysis. MS spectra, acquired using an MS detector, may be interactively selected from a chromatogram signal for visual inspection and comparison. The spectra may also be used for peak purity determinations and component identification through spectral libraries.
It is possible to import MS data in AIA (cdf), mzXML, MzML, and mzData formats.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_software_Mass_Spectrometry_Extension_2_6b036064d3/DataApex-Clarity-software-Mass-Spectrometry-Extension-2.webp" alt="DataApex: Clarity software - Mass Spectrometry Extension"/>
Control modules for selected detectors (see list of <a href="https://www.dataapex.com/controls">control modules</a>) are available in Clarity.
Additional control modules for MS detectors can be developed upon request, please <a href="https://www.dataapex.com/document/contacts">contact us</a> for availability.
<blockquote>
<h3>Selected supported GC/MS &amp; LC/MS detectors</h3>
</blockquote>
<blockquote>
<h5>LC-MS Detectors</h5>
<ul>
<li>Advion: Expression</li>
<li>Gilson: Verity 1920</li>
<li>Konik: Q4 LCMS</li>
<li>Microsaic: 4000 MiD</li>
<li>Microsaic: 4500 MiD</li>
<li>Young In Chromass: YL9900</li>
</ul>
</blockquote>
<blockquote>
<h5>GC-MS Detectors</h5>
<ul>
<li>Young In Chromass: ChroZen GC/MS</li>
<li>Young In Chromass: YL6900</li>
</ul>
</blockquote>
<blockquote>
<h5>GC-MS TOF Detectors</h5>
<ul>
<li>Markes International: BenchTOF</li>
</ul>
</blockquote>

Clarity software - Mass Spectrometry Extension

DataApex founder and general manager Jan Hrubý explains how a combination of serendipity and a spike of post-communist innovation led to the birth of company that spent 30 years perfecting data acquisition, processing, and instrument control software to give us <a href="https://www.dataapex.com/product/clarity-std">Clarity</a>.
<h5>Tell us about the genesis of DataApex…</h5>
I was born and raised in communist Czechoslovakia, which was occupied by the Soviet army until I turned four. My great love and hobby since childhood was electronics and later computers, which I eventually studied at university in the 1980s – though I never actually saw a computer with my own eyes the whole time! Immediately after the fall of communism in 1989, my friends and I started thinking about starting a business.
But I suppose it was my then mother-inlaw who was really responsible for founding DataApex. She was an excellent scientist, but her instrumentation was limited. In the early 1990s, she asked me if I could build a better integrator for her – in other words, a tool for measuring and evaluating chromatograms.
Sometimes things come together at the right time in the right place. My best friend – an excellent programmer – was studying for his PhD at the University of Science and Technology, Czech Republic, and his friend was writing his thesis on modern integration algorithms at the same time. So, a decision was made, and six months later we handed over to my mother-in-law the first working version of the PC software, including our own A/D converter.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Zakladatel_a_generalni_reditel_Data_Apex_Jan_Hruby_24c940f666/DataApex-Zakladatel-a-generalni-reditel-DataApex-Jan-Hruby.jpg" alt="DataApex:Founder and CEO of DataApex Jan Hrubý"/>
<h5>What challenges did you face starting a software company from scratch in those times?</h5>
There were many challenges; where should I start? First, a computer in the early 1990s cost about 10–20 monthly salaries; we couldn’t afford to buy one, so we took turns using one at the university. Second, A/D converter parts were also pricey (for us), so we had to visit the electronic bazaars in Munich to drive down the cost. Third, because private business was forbidden in Czechoslovakia for 40 years until 1989, we didn’t have many mentors to learn from. The list is long but, fortunately, we were young and full of optimism about the freedom we had gained, so we embraced all the challenges!
<h5>Tell us about your chromatography software, Clarity, and the company culture…</h5>
I can say with some satisfaction that Clarity is a mature product after thirty years of development. The current generation – our third iteration – speaks six languages, runs over 1,000 chromatography instruments across almost every manufacturer around the world, and is sold in more than 100 countries.
Though we are a single product company, we have several variants of Clarity that cover the needs of diverse customers; we have simple and affordable software for legacy chromatographs (it is often impossible to find original software), an educational version for universities, and more sophisticated solutions for tightly regulated markets and GC/LC-MS instruments, for instance. But all variants share one thing in common: they are reliable and easy to use.
And because we’re a single product company, all staff are united by a singular focus, which results in a family-like atmosphere and a level of dedication that is otherwise hard to cultivate. As a result, our customers are often surprised by how quickly our support team reacts – sometimes they ask if our support colleagues get any sleep. (Yes, they do!).
Finally, our chromatography instrument manufacturer partners know and trust that we are – and want to continue to be – an independent and vendor-neutral company.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Founder_and_CEO_of_Data_Apex_Jan_Hruby_and_his_great_hobby_yachting_39f5593220/DataApex-Founder-and-CEO-of-DataApex-Jan-Hruby-and-his-great-hobby-yachting.jpg" alt="DataApex: Founder and CEO of DataApex Jan Hrubý and his great hobby yachting"/>
<h5>Where can people find out more?</h5>
Our company policy is very open and virtually all information is available on <a href="https://www.dataapex.com/">our website dataapex.com</a>. We post all news on social media, especially <a href="https://www.linkedin.com/company/dataapex/?trk=public_profile_topcard_current_company">on LinkedIn</a>, and there are dozens of short tutorial videos and brief video tips and tricks available <a href="https://www.youtube.com/user/dataapex">on YouTube</a>, for example. We have an extensive network of trained distributors all over the world. We also offer <a href="https://www.dataapex.com/download?product_filter=2">demos or free trial versions</a>.
<h5>What are your hopes for the field – and for DataApex?</h5>
I long for a unified communication interface for all analytical instruments that is independent of manufacturers and available to all – as well as a uniform analytical data format for easy exchange and sharing among software platforms. Everyone in the industry is calling for both – but, for many reasons (some obvious and some unclear), neither are forthcoming.
Finally (and mainly), I dream of delivering Clarity to a lab on Mars!

Supporting Chromatography Everywhere – Even Mars

<h3>Introduction</h3>
Besides its use in analytical GC, HPLC and CE, the Clarity chromatography software is well suited also for control of preparative chromatography systems. Using the FCGP (General Purpose Fraction Collector) control module, many different fraction collectors could be controlled by the simple Next and Collect/Waste commands generated by the module and transferred to the devices either through the digital Outputs/Inputs or by suitable communication line (RS232, LAN, GSIOC). Not only dedicated fraction collectors, but also multi-position valves could be controlled. The fraction collection parameters (including time windows and signal level or slope triggered collection) are defined within the Clarity FC-GP method setup. 
Two examples of automated systems used for isolation and purification of antibodies, based on common instrumentation and controlled by Clarity chromatography software are presented.
<h3>Automated Immunoaffinity Chromatography</h3>
This instrument setup is designed to isolate specific antibodies from clarified serum. The serum is obtained from an animal immunized with the antigen which we want to generate antibodies against. The media in the column has the same or a similar antigen covalently bound to it, in order to capture antibodies which are specific to that antigen. 
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_1_Diagram_of_the_immunoaffinity_separation_system_2453e733ba/DataApex-Fig.-1-Diagram-of-the-immunoaffinity-separation-system.jpg" alt="DataApex: Fig. 1 – Diagram of the immunoaffinity separation system"/>
The instrument is configured around our existing lab infrastructure (benches, racks, shelves, rods for mounting columns, etc.). A 6 position selector valve allows selection of serum, a wash buffer, an elution buffer, or a column cleaning buffer. The unused positions are plugged so that they can be selected to put the instrument into a standby state which prevents any siphoning of the mobile phases due to gravity. 
The output of the selector valve leads to the pump. Immediately after the pump is a backpressure regulator to ensure proper seating of the check valves and a second one, serving as a pressure relief valve, as the pump does not feature pressure monitoring.
The tubing is then plumbed into a 6 port, 2 position valve, used to allow the direction of flow through the column to be reversed (see plumbing diagram). This allows strongly retained antibodies at the top of the column to be eluted quickly, with minimal exposure to the harsh elution buffer. After flowing through the column, the mobile phase returns to the valve and is directed through pH and UV detectors to a second 6 position selector valve, where the output can be directed to reservoirs for waste, eluted material, or material to be reloaded for further processing. 
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_2_Example_of_the_elution_cycle_in_immunoaffinity_separation_09c29ecf9a/DataApex-Fig.-2-Example-of-the-elution-cycle-in-immunoaffinity-separation.jpg" alt="DataApex: Fig. 2 - Example of the elution cycle in immunoaffinity separation"/>
Currently we have 13 instruments in service that match this general description. Each instrument takes up roughly 50 cm x 50 cm of bench space, making use of shelving above the bench to hold large reservoirs. The “prototype” for this instrument design was assembled primarily from components we had in our lab that were not in use.
The full version of Clarity with the LC module is used on all instruments in order to allow for expansion. Most of the stations already have the maximum number of 4 instruments installed. Serial communication ports for the valves and pumps are provided by a DataApex Multicom device. All valves are mounted on Valco Microelectric Actuators with RS-232 communication. Analog signals from the pH probe and UV absorbance detector are acquired using either an INT9 A/D PCI card, a 4 channel Colibrick device, or a 2 channel UPAD, depending on the instrument. The PCs used are from various manufacturers, or are built to specifications in house.
<h5>Table 1- Immunoafinity System Components</h5>
<h5>No. / Name / Description</h5>
<ol>
<li>Reservoirs / Sample, Wash, Eluent, Column Cleaning</li>
<li>Selection valve / 6 position selector valve, (Valco, C25-6186EMH)</li>
<li>Pump / SSI/LabAlliance Series 1 Pump (Chromtech, P-040), with 40 ml/min heads</li>
<li>Backpressure regulator / 40 psi, to provide backpressure for pump valves</li>
<li>Backpressure regulator / 100 psi, connected by a tee, serving as pressure relief valve</li>
<li>Switching valve / 6 port, 2 position valve (Valco C22-6186EH)</li>
<li>Column / 5 cm diameter x 5 cm length</li>
<li>pH detector / Flow cell (Sensorex, FC45C) with Flat pH electrode (Sensorex, S450CD)</li>
<li>UV detector / UV absorbance detector (Bio-Rad, EM-1)</li>
<li>Selection valve / 6 position selector valve, (Valco, C25-6186EMH)</li>
<li>Reservoirs / Waste, Pure Product, Impure Product for reloading</li>
</ol>
1/8” ID Dupont PFA tubing is used throughout for connections
Multiple isolation cycles are needed to process the starting material quantity. The sequence table is thus used to control the instrument. Separate methods are written for the loading, washing, elution, and cleaning phases of the process. These methods are then repeated in the sequence to create cycles, which can be performed automatically until the desired amount of material has been processed. Sequences can also be run overnight, with the primary limitation being the size of the mobile phase and collection reservoirs. 
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_3_Example_of_sequence_table_for_immunoaffinity_separation_58a994a3fe/DataApex-Fig.-3-Example-of-sequence-table-for-immunoaffinity-separation.jpg" alt="DataApex: Fig. 3 - Example of sequence table for immunoaffinity separation"/>
<h3>Preparative size exclusion chromatography with stacked injections using Clarity</h3>
This instrument setup is designed to facilitate removal of aggregates and other high molecular weight species from antibodies. Generally, the harshness of the elution buffer used in the affinity step leads to some degree of aggregation in the isolated antibodies.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_4_Diagram_of_the_Stacked_Injection_SEC_system_a7622a7986/DataApex-Fig.-4-Diagram-of-the-Stacked-Injection-SEC-system.jpg" alt="DataApex: Fig. 4 - Diagram of the Stacked Injection SEC system"/>
A 6 position selector valve is used to switch between a sample reservoir and a running buffer reservoir. This is connected to a pump, with backpressure regulator configured as a blow-off valve in order to protect the column from excessive pressure in the event of a clog. The outlet of the column is connected to a UV absorbance detector. Fractions are collected using a fraction collector. 
<h5>Table 2 - Size Exclusion System Components</h5>
<h5>No. / Name / Description</h5>
<ol>
<li>Reservoirs / Sample, Eluent, Column Cleaning</li>
<li>Selection valve / 6 position selector valve, (Valco, C25-6186EMH)</li>
<li>Pump / SSI/LabAlliance Series 1 Pump (Chromtech, P-040), with 40 ml/min heads</li>
<li>Backpressure regulator/ 40 psi, to provide backpressure for pump valves</li>
<li>Backpressure regulator / 100 psi, connected by a tee, serving as pressure relief valve</li>
<li>Column / 5 cm x 100 cm (GE, XK-50/100) column packed with Superdex 200 Prep Grade.</li>
<li>UV detector / UV absorbance detector (Bio-Rad, EM-1)</li>
<li>Fraction collector / Isco Foxy 200 fraction collector</li>
</ol>
1/8” ID Dupont PFA tubing is used throughout for connections.
Again, multiple injections are needed to load all the sample to be processed. In order to reduce the time required to process large samples, a stacked injection technique is used. As a run begins, the solvent selection valve loads the first portion of the sample from the sample reservoir then switches back to the running buffer.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_5_Example_of_Stacked_Injection_Method_Event_Table_687cda6e11/DataApex-Fig.-5-Example-of-Stacked-Injection-Method-Event-Table.jpg" alt="DataApex: Fig. 5 - Example of Stacked Injection Method Event Table"/>
After the first portion has migrated some distance down the column, the valve switches to the sample reservoir loading a second portion. After the second portion has migrated down the column a third can be loaded, and so on. If the characteristics of the sample are known, spacing between samples can be reduced significantly versus loading one sample and waiting for it to elute completely. In one example, a single sample takes 165 minutes to elute completely, however 8 samples can be processed in 790 minutes, saving almost 9 hours by using stacked injections. Multi-gram quantities of material can be processed per day using this technique. 
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_6_Example_of_stacked_injection_SEC_separation_019f37638a/DataApex-Fig.-6-Example-of-stacked-injection-SEC-separation.jpg" alt="DataApex: Fig. 6 - Example of stacked injection SEC separation"/>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_7_Detail_of_stacked_injection_SEC_separation_cycle_bc1be53e3f/DataApex-Fig.-7-Detail-of-stacked-injection-SEC-separation-cycle.jpg" alt="DataApex: Fig. 7 - Detail of stacked injection SEC separation cycle"/>
<h3>Conclusion</h3>
We have demonstrated the use of Clarity in purification of antibodies using two specific setups, the automated immunoaffinity chromatography and preparative size exclusion chromatography with stacked injections.
Similar setups can be used with large range of fraction collectors and multi-position valves. The description can also help with setting up systems for other preparative applications.
<h3>Featured Clarity products</h3>
In both applications the same Clarity Chromatography Software system has been used:
<h5>Table 3 - Clarity Software Components (Item - p/n)</h5>
<ul>
<li>Clarity Single Instrument - C50</li>
<li>LC Control Module - A24</li>
<li>AD Converters- e.g. Colibrick 4 ch - U34</li>
<li>Add on instruments (optional – to control more systems from 1 station) - C55</li>
</ul>

Automated SW solution for preparative HPLC

<h3>The recommended procedure for H53 method integration in Clarity (method 1):</h3>
<ol>
<li>Use Baseline – Lock on the whole chromatogram.
</li>
<li>Apply Baseline – Allow Crossing to the whole chromatogram with the option To Start/End.
</li>
<li>Via Peak – Add positive, add the following peaks:
</li>
</ol>
<ul>
<li>A peak with the start time just before the solvent peak (this is the initial point of the baseline) and the end time at the beginning of the first fraction.</li>
<li>The first fraction (C10) peak with the start time exactly at the end time of the previous peak and the end time at the end of the fraction.</li>
<li>In a similar manner, i.e., with non-overlapping time frames, add the other peaks for remaining fractions (optionally, C10–C20, C20–C30, and C30–C40).</li>
</ul>
<ol start="4">
<li>Set Baseline – Together and Baseline – Forward horizontal over the entire chromatogram.
</li>
<li>Assign peaks to respective fractions using Force Peak Name (exact times are filled automatically when selecting the peak from the graph).
</li>
<li>Create a calibration file for the present fractions.
</li>
</ol>
When the chromatogram is integrated appropriately, you can use the Method - Save as Template command from the Chromatogram window to save the method as a template method, so it can be used for further measurements. In the case you have already measured all the samples, you can apply the integration on all your chromatograms via Analysis – Batch reprocess by method.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_How_to_determine_non_volatile_hydrocarbons_according_to_DIN_H53_ISO_16703_1_4d7e6aabf8/DataApex-How-to-determine-non-volatile-hydrocarbons-according-to-DIN-H53-(ISO-16703)-1.jpg" alt="DataApex - How to determine non-volatile hydrocarbons according to DIN H53 (ISO 16703)? 1"/>
Note: In the case, that the amounts of some compounds in standards are not known, it is necessary to set the Curve Fit Type to Free Calibration on the tabs of these compounds in the Calibration window.
<h4>Example of an evaluated chromatogram</h4>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_How_to_determine_non_volatile_hydrocarbons_according_to_DIN_H53_ISO_16703_2_9da75db54b/DataApex-How-to-determine-non-volatile-hydrocarbons-according-to-DIN-H53-(ISO-16703)-2.jpg" alt="DataApex - How to determine non-volatile hydrocarbons according to DIN H53 (ISO 16703) - 2"/>
<h3>An alternative procedure for H53 method integration in Clarity (method 2):</h3>
<ol>
<li>Use the same Peak Width and Threshold as in standard integration.
</li>
<li>Apply Baseline – Together to the whole chromatogram.
</li>
<li>Apply Baseline – Forward Horizontal to the whole chromatogram.
</li>
<li>Mark all the peaks between C10 and C40 as a group (the group boundary lines may exceed the C10 and C40 peaks, only fully contained peaks will be included).
</li>
<li>Amend the peak starts and ends by manual peak tools if necessary for the C10 and C40 (use the Peak - Both, with the A time corresponding to the respective C10 or C40 time and B time positive or negative, respectively. The times are relative, i.e., will be shifted accordingly in other chromatograms).
</li>
<li>Create calibration file for C10, C40, and Group A peaks.
</li>
</ol>
If the integration is not correct at this point (e.g., the integrated area for Group A between C10 and C40 peaks does not start/end exactly where the C10/C40 peaks end/start respectively, as shown in the figure below), an additional step might be added after step 3.: Apply again Baseline – Together to the whole chromatogram. Continue with the remaining steps.
To subtract a baseline, in the Method Setup – Advanced dialog, set the baseline chromatogram, and in the Matching, set No Change. The baseline file should be acquired on the stabilized system and preferably without injection of solvent or at least with a lower volume of the solvent. Otherwise, negative artifacts will appear, interfering with the correct baseline setting in Clarity later on.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_How_to_determine_non_volatile_hydrocarbons_according_to_DIN_H53_ISO_16703_3_cbe418722f/DataApex-How-to-determine-non-volatile-hydrocarbons-according-to-DIN-H53-(ISO-16703)-3.jpg" alt="DataApex - How to determine non-volatile hydrocarbons according to DIN H53 (ISO 16703) - 3"/>
<blockquote>
There is a condition that must be met:
</blockquote>
<blockquote>
<ul>
<li>The drawn baseline must not cross the chromatogram line (the negative areas arising do not have any physical meaning).</li>
</ul>
</blockquote>
This approach is generally less reliable and more sensitive to the choice of Integration Algorithm. 
<h4>Example of an evaluated chromatogram</h4>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_How_to_determine_non_volatile_hydrocarbons_according_to_DIN_H53_ISO_16703_4_46736a7950/DataApex-How-to-determine-non-volatile-hydrocarbons-according-to-DIN-H53-(ISO-16703)-4.jpg" alt="DataApex - How to determine non-volatile hydrocarbons according to DIN H53 (ISO 16703) - 4"/>
<h3>Sample data for Clarity</h3>
<ul>
<li><a href="https://downloads.dataapex.com/documentation/clarity/other/DIN_H53.zip">DIN_H53.zip</a> - zipped chromatograms, methods, and calibrations.</li>
<li><a href="https://downloads.dataapex.com/documentation/clarity/other/Din-H53-processed.dgz">Din-H53-processed.dgz</a> - archived Clarity Project to restore with Clarity via the Archive/Restore function</li>
</ul>

How to determine non-volatile hydrocarbons according to DIN H53 (ISO 16703)?

<h3>We are pleased to inform you about the enhanced control capabilities offered through the <a href="https://lcms.labrulez.com/products?manufacturerItems=95&amp;search=Clarity">Clarity software family</a>.</h3>
<h3>Clarity - Shimadzu Edition</h3>
Clarity - Shimadzu Edition expands instrument control options of Clarity Chromatography Software with <a href="https://lcms.labrulez.com/library?instrumentationItems=27&amp;mainauthorItems=1&amp;search=Nexera">Shimadzu Nexera HPLC</a> and <a href="https://gcms.labrulez.com/library?instrumentationItems=1&amp;mainauthorItems=1&amp;search=Nexis">Nexis GC systems</a>.
In addition to all supported instruments in Clarity, this edition brings exclusive control of Shimadzu Nexera HPLC and Nexis GC systems. 
The control of Shimadzu Nexis GC-2030 GC and Shimadzu Nexera HPLC is available only through a special Clarity OEM version called Clarity - Shimadzu Edition (p/n C50-28) which is available only in Europe.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_Shimadzu_Edition_13a618e618/DataApex-Clarity-Shimadzu-Edition.jpg" alt="DataApex: Clarity - Shimadzu Edition"/>
<h4>All Clarity features in the Shimadzu edition</h4>
You will get all the optional features available in Clarity with your Shimadzu edition, such as integrated control of selected instruments (GCs, LCs, pumps, detectors, autosamplers, etc.). Extensions provide functions for specific separation techniques or calculations such as <a href="https://lcms.labrulez.com/products/1001">PDA</a>, <a href="https://lcms.labrulez.com/products/1004">GPC analysis</a>, <a href="https://lcms.labrulez.com/products/1003">Mass spectrometry</a>, or <a href="https://lcms.labrulez.com/products/1002">SST</a>. User interface choice of languages includes English, French, German, Russian, Spanish, and Chinese. Our highly rated technicians and developers provide timely, extensive support.
<h3>Control module for <a href="https://gcms.labrulez.com/library?instrumentationItems=1&amp;mainauthorItems=1&amp;search=Nexis+2030">Shimadzu Nexis GC-2030</a> system</h3>
The Shimadzu Nexis GC-2030 Control module allows direct control of this GC (Gas chromatograph) using Agilent ICF (Instrument Control Framework). Direct control means that the GC can be completely controlled from the Clarity - Shimadzu Edition Chromatography Station enabling integrated GC control thus ensuring complete automation for laboratories. The instrument method controlling the analysis conditions will be saved in measured chromatograms. Along with the above-mentioned CG, the control module also allows controlling autosamplers operated through the GC. The control is performed via LAN. The minimum required firmware of the GC is version 1.09.
<h4>Specification</h4>
Shimadzu Nexis GC-2030
<ul>
<li>Controlled instruments: Nexis GC-2030 gas chromatograph, AOC-20 family autosamplers</li>
<li>Supported detectors: FID, TCD, FTD, FPD, ECD, BID</li>
<li>Communication interface: LAN</li>
<li>Purchasing: GC Control - (p/n A23), GC Shimadzu Control (p/n A23-001) - needs to be purchased once for each connected system, AS Control - (p/n A26) - when autosampler is present</li>
<li>Related products: Clarity - Shimadzu Edition (p/n C50-28) Only available in Europe</li>
<li>Cable: Cross LAN (p/n SK08)</li>
</ul>
<h3>Control module for <a href="https://lcms.labrulez.com/library?instrumentationItems=27&amp;search=Shimadzu+Nexera+HPLC">Shimadzu Nexera HPLC</a> systems</h3>
Direct control of Shimadzu Nexera HPLC components using the Agilent ICF (Instrument Control Framework) tools developed by the Agilent company. The Clarity Chromatography Station - Shimadzu Edition allows for direct control of selected Shimadzu Nexera components (mostly from the Nexera LC-40 line), enabling Clarity to provide integrated instrument control and to ensure complete automation for laboratories. The control module includes parameters for the HPLC pumps, column ovens, detectors (including PDA), autosamplers, etc. A dedicated PDA Extension enables the evaluation of data from PDA systems.
The control module limits the usage of multiple Nexera HPLC systems in the same Clarity - only a single system is guaranteed to function reliably.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Shimadzu_Nexis_GC_2030_Control_10da3b4ef0/DataApex-Shimadzu-Nexis-GC-2030-Control.jpg" alt="DataApex: Shimadzu Nexis GC-2030 Control"/>
<h4>Specification</h4>
Shimadzu Nexera Control
<ul>
<li>Controlled devices: The Shimadzu Nexera HPLC control module is compatible with selected components of Shimadzu Nexera HPLC systems supported by the Agilent ICF. Exact list of controlled components is shown in the list below.</li>
<li>Communication interface: LAN</li>
<li>Data acquisition interface: Digital Data Acquisition Purchasing: LC Control (p/n A24), LC Shimadzu Control (p/n A24-001) - needs to be purchased once for each connected system, AS Control (p/n A26) - optional, PDA Extension (p/n A29) - optional</li>
<li>Special: Based on controller serial number and unique LC Shimadzu Control license number, specific Connect Key is needed for the LC to communicate, for more details see the manual.</li>
<li>Related products: Clarity - Shimadzu Edition (p/n C50-28)</li>
<li>Cable: Cross LAN (p/n SK08)</li>
</ul>
List of controlled subdevices of the Shimadzu Nexera control module. Please note that the portfolio of components widens with further releases of newer control module versions.
<ul>
<li>Controllers: CBM-40, CBM-40 Lite, SCL-40</li>
<li>Pumps: LC-40B X3, LC-40B XR, LC-40D, LC-40D X3, LC-40D XR, LC-40D XS</li>
<li>Detectors: CDD-10Avp, RF-20A, RF-20AXS, RID-20A, SPD-40, SPD-40V</li>
<li>PDA Detectors: SPD-M30A, SPD-M40</li>
<li>Autosamplers: SIL-40, SIL-40 XR, SIL-40C, SIL-40C X3, SIL-40C XR, SIL-40C XS</li>
<li>Column Ovens: CTO-30A, CTO-40C, CTO-40S</li>
<li>Other: Mobile Phase Monitor MPM-40</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Shimadzu_Nexera_Control_1a5d578355/DataApex-Shimadzu-Nexera-Control.jpg" alt="DataApex: Shimadzu Nexera Control"/>
<a href="https://www.dataapex.com/controls">List of all supported instruments</a>
<blockquote>
<a href="https://www.dataapex.com/product/clarity-shimadzu-edition-demo">Demo version available</a>
</blockquote>
<blockquote>
Preview functions of Clarity - Shimadzu Edition. You can try any controlled instrument in simulated demo mode without connecting to a real device. Discover all configuration options and run simulated data acquisition. A stored demo data set allows you to try the capabilities of data processing.
</blockquote>

Nexera HPLC and Nexis GC systems  Supported in Clarity

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Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

Nexera HPLC and Nexis GC systems Supported in Clarity

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