<p><strong>Symposium &quot;Advances in Chromatography and Electrophoresis &amp; Chiranal 2022&quot; belongs to the periodical meetings of specialists working in the field of separation methods.</strong></p>
<p>The biennial conference is organized by Department of Analytical Chemistry, Faculty of Science, <a href="https://lcms.cz/companies/17"><strong>Palacky University</strong></a> in cooperation with Regional Centre of Advanced Technologies and Materials, Czech Chemical Society and <a href="https://lcms.cz/companies/21"><strong>Czech Society for Mass Spectrometry</strong></a>. It is organized as a meeting of the scientists from the universities, academic institutes and experts from the routine analytical laboratories.</p>
<p>The scientific program will deal with all areas of separation science <strong>from fundamental to applied reseach</strong> in chemistry, physics, biology, medicine, forensic science and related disciplines. Official languages of the conference are <strong>English</strong>, <strong>Czech</strong> and <strong>Slovak</strong>.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UPOL_Katedra_analyticke_chemie_Pr_F_UP_Advances_in_Chromatography_and_Electrophoresis_and_Chiranal_2022_Programme_5817ff6a89/UPOL-Katedra-analyticke-chemie-PrF-UP-Advances-in-Chromatography-and-Electrophoresis-and-Chiranal-2022-Programme.webp" alt="UPOL: Katedra analytické chemie PřF UP - Advances in Chromatography and Electrophoresis &amp; Chiranal 2022 - Programme"/></p>
<h3><strong>Programme</strong></h3>
<h3><strong>Monday 13th June</strong></h3>
<h5><strong>14:00 – 17:30 Registration</strong></h5>
<ul>
<li>Fort Science, foyer, ground floor</li>
<li>Individual arrival of participants and accommodation</li>
</ul>
<h5><strong>16:00 – 16:15 Opening of conference</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
<li>Presence of official representatives of Palacký University and Czech Chemical Society</li>
<li><strong>Foreword: Petr Bednář</strong></li>
</ul>
<h5><strong>16:15 – 17:15 Plenary Lecture</strong> (Chairmen: <a href="https://lcms.cz/articles?search=Karel+Lemr">Karel Lemr</a>, Juraj Ševčík)</h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<h5><strong>Innovations in microchip electrophoresis: from design to applications. Honoring the memory of Prof. Milan Hutta</strong></h5>
<ul>
<li><strong>Marián Masár</strong></li>
</ul>
<h5><strong>17:30 – ?? Welcome party + banquet</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<h3><strong>Tuesday 14th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
<li>Installation of posters (Faculty of Science, seminary rooms, 2nd floor)</li>
</ul>
<h5><strong>09:00 – 11:00 Lectures I: Electromigration methods and chip technologies</strong> (Chairmen: Michael Lämmerhofer, Petr Bednář)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Electrokinetic and microchip design for proteomics, interactomics and diagnostics</strong></h5>
<ul>
<li><strong>Anne Varenne</strong></li>
</ul>
<h5><strong>09:30 – 10:00 Non-invasive samples in clinical diagnostics: Can capillary electrophoresis be a suitable diagnostic tool?</strong></h5>
<ul>
<li><strong>Petr Kubáň</strong></li>
</ul>
<h5><strong>10:00 – 10:20 Chiral capillary electrophoresis with diquats as both passive and active players in the enantioseparation games</strong></h5>
<ul>
<li><strong>Václav Kašička</strong></li>
</ul>
<h5><strong>10:20 – 10:40 Simul 6 – A fast simulation software for electrophoresis</strong></h5>
<ul>
<li><strong>Bohuslav Gaš</strong></li>
</ul>
<h5><strong>10:40 – 11:00 Monolithic silicas in high performance liquid chromatography – unique material with lots of benefits</strong></h5>
<ul>
<li><strong>Egidijus Machtejevas</strong> (Merck)</li>
</ul>
<h5><strong>11:00 – 11:20 How we had fun during the lockdown, challenges that new products brought</strong></h5>
<ul>
<li><strong>Luděk Vlk</strong> (Chromservis)</li>
</ul>
<h5><strong>11:20 – 11:40 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>11:40 – 13:40 Lectures II: Multidimensional chromatography, mass spectrometry of small molecules</strong> (Chairmen: Petr Kubáň, Petr Barták)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:40 – 12:10 Multidimensional liquid chromatography and pharmaceutical applications</strong></h5>
<ul>
<li><strong>Michael Lämmerhofer</strong></li>
</ul>
<h5><strong>12:10 – 12:40 Novel approaches to overcome dilution in comprehensive two-dimensional liquid chromatography</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Petr+%C4%8Cesla"><strong>Petr Česla</strong></a></li>
</ul>
<h5><strong>12:40 – 13:00 Application of flow-modulated comprehensive two-dimensional gas chromatography in chiral analysis of wine</strong></h5>
<ul>
<li><strong>Olga Vyviurska</strong></li>
</ul>
<h5><strong>13:00 – 13:20 Pharmaceutical analysis in the development of water-soluble prodrug of ICRF–193, a protective agent against anthracycline cardiotoxicity</strong></h5>
<ul>
<li><strong>Petra Štěrbová-Kovaříková</strong></li>
</ul>
<h5><strong>13:20 – 13:40 Achieving robust profiling, sensitive quantitation and confident unknown identification for small molecules applications with <a href="https://lcms.cz/products/403">Orbitrap Exploris 120 MS</a></strong></h5>
<ul>
<li><strong>Maciej Bromirski</strong> (<a href="https://lcms.cz/companies/42">Pragolab</a>)</li>
</ul>
<h5><strong>13:40 – 14:40 Lunch</strong></h5>
<ul>
<li>Mensa</li>
</ul>
<h5><strong>14:40 – 16:00 Poster session I (odd poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h5><strong>16:00 – 17:50 Lectures III: Current trends in chromatography</strong> (Chairmen: Václav Kašička, Ondřej Kurka)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>16:00 – 16:30 Comparison of lipid class and lipid species separation approaches in the lipidomic analysis</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Michal+Hol%C4%8Dapek"><strong>Michal Holčapek</strong></a></li>
</ul>
<h5><strong>16:30 – 17:00 Miniaturization as one of the trends in modern analytical chemistry</strong></h5>
<ul>
<li><strong>Zeineb Aturki</strong></li>
</ul>
<h5><strong>17:00 – 17:20 The possibilities of chiral separation of anionic boron clusters</strong></h5>
<ul>
<li><strong>Radim Kučera</strong></li>
</ul>
<h5><strong>17:20 – 17:40 Electron activated dissociation as potential solution to identification and quantitation of isomers using mass spectrometry without additional separation techniques</strong></h5>
<ul>
<li><strong>Tomáš Korba</strong> (Amedis)</li>
</ul>
<h5><strong>17:40 – 18:00 HPLC separation in metabolomic studies: challenges and solutions</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Ond%C5%99ej+Lacina"><strong>Ondřej Lacina</strong></a> (<a href="https://lcms.cz/companies/37">HPST</a>)</li>
</ul>
<h5><strong>18:00 – 19:00 Dinner</strong></h5>
<ul>
<li>Mensa</li>
</ul>
<h5><strong>19:30 – 21:00 Informal evening visit to the laboratories of the Department of Analytical Chemistry – including the Cyclic ion mobility MS lab</strong></h5>
<ul>
<li><a href="https://lcms.cz/products/202"><strong>SELECT SERIES Cyclic IMS instrument</strong></a>, third installed instrument with this unique technology worldwide</li>
</ul>
<h3><strong>Wednesday 15th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>09:00 – 11:20 Lectures IV:  Applications and practice of chromatography, heritage science, forensic science</strong> (Chairmen: Radim Kučera, Lukáš Kučera)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Art of chromatography – Chromatography in art</strong></h5>
<ul>
<li><strong>Václav Pitthard</strong></li>
</ul>
<h5><strong>09:30 – 10:20 Forensic applications of analytical chemistry</strong></h5>
<ul>
<li><strong>Luboš Valerián, Jan Aufart</strong></li>
</ul>
<h5><strong>10:20 – 10:40 Cyclic ion mobility – mass spectrometry of isomeric cathinones</strong></h5>
<ul>
<li><strong>Marianna Nytka</strong></li>
</ul>
<h5><strong>10:40 – 11:00 Intact protein analysis in biological fluids by liquid chromatography – triple quadrupole mass spectrometry</strong></h5>
<ul>
<li><strong>Katarína Maráková</strong></li>
</ul>
<h5><strong>11:00 – 11:20 Practical approaches for identifying equivalent and orthogonal columns</strong></h5>
<ul>
<li><strong>Heiko Behr</strong> (Phenomenex)</li>
</ul>
<h5><strong>11:20 – 13:00 Poster session II and coffee break</strong></h5>
<ul>
<li>Even poster numbers</li>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h5><strong>13:00 – 14:00 Lunch</strong></h5>
<ul>
<li>Mensa</li>
</ul>
<h5><strong>15:00 – 17:00 Social/sport event</strong></h5>
<ul>
<li>Bowling at Bowland Olomouc</li>
<li>A tour of Regional Office of National Heritage Institute (see details at the bottom of the page)</li>
</ul>
<h5><strong>18:00 – ?? Social dinner</strong></h5>
<ul>
<li>Faculty of Science, foyer, 6th floor</li>
</ul>
<h3><strong>Thursday 16th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>09:00 – 10:30 Lectures V: Supercritical fluid chromatography, pharmaceutical analysis</strong> (Chairmen: Dalibor Šatínský, Petr Fryčák)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Supercritical fluid chromatography mass spectrometry coupling: where are we?</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Lucie+Nov%C3%A1kov%C3%A1"><strong>Lucie Nováková</strong></a></li>
</ul>
<h5><strong>09:30 – 10:00 Reaction of basic analytes with carbon dioxide under supercritical fluid chromatography conditions: Confirmed</strong></h5>
<ul>
<li><strong>Michal Kohout</strong></li>
</ul>
<h5><strong>10:00 – 10:20 Unified chromatography – a step towards universal chromatographic methods</strong></h5>
<ul>
<li><strong>Volodymyr Pauk</strong></li>
</ul>
<h5><strong>10:20 – 10:40 Control of N-Nitrosamines in Pharmaceuticals</strong></h5>
<ul>
<li><strong>Jakub Jireš</strong></li>
</ul>
<h5><strong>10:40 – 11:00 Non-targeted UHPLC-DIA-HRMS approach for examination of impurities in levothyroxine formulations</strong></h5>
<ul>
<li><strong>Hana Chmelařová</strong></li>
</ul>
<h5><strong>11:00 – 11:20 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>11:20 – 13:30 Lectures VI: Novelty in sample preparation, ion mobility, data treatment</strong> (Chairmen: Lucie Nováková, Volodymyr Pauk)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor)</li>
</ul>
<h5><strong>11:20 – 11:50 Advanced extraction approaches to sample extraction in flow and chromatography techniques based on nanofibers</strong></h5>
<ul>
<li><strong>Dalibor Šatínský</strong></li>
</ul>
<h5><strong>11:50 – 12:10 DisperSpin solid phase micro-extraction as a novel tool of phytohormonal analysis</strong></h5>
<ul>
<li><strong>Ivan Petřík</strong></li>
</ul>
<h5><strong>12:10 – 12:30 Monitoring of PAHs in simulated natural and artificial fires by HPLC-DAD-FLD with the application of Multi Component Integrated Calibration Method to improve quality of analytical results</strong></h5>
<ul>
<li><strong>Paweł Świt</strong></li>
</ul>
<h5><strong>12:30 – 12:50 Ion-mobility coupling with desorption electrospray ionization mass spectrometry imaging in plant isomer research</strong></h5>
<ul>
<li><strong>Chao Zhang</strong></li>
</ul>
<h5><strong>12:50 – 13:10 High resolution ion mobility separation</strong></h5>
<ul>
<li><strong>Alexandr Muck</strong> (<a href="https://lcms.cz/companies/50">Waters</a>)</li>
</ul>
<h5><strong>13:10 – 13:30 Using trapped ion mobility spectrometry to improve confidence in identification of knowns and unknowns</strong></h5>
<ul>
<li><strong>Noud van der Borg</strong> (Bruker)</li>
</ul>
<h5><strong>13:30 – 14:10 Closing of conference and farewell drink</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>14:10 – 15:10 Lunch</strong></h5>
<ul>
<li>Mensa</li>
</ul>
<h4><strong>Social programme:</strong></h4>
<h5><strong>A tour of Regional Office of National Heritage Institute</strong></h5>
<p>The Archaeological Department of the National Heritage Institute, Regional Office in Olomouc has been carrying out excavations in the territory of the former capital of Moravia for 50 years. In recent years, interdisciplinary cooperation with natural scientists – archaeobotanists, palynologists, anthropologists and, last but not least, chemists from the Faculty of Science of Palacký University – has been successfully developing. Examples of jointly researched artefacts include a prehistoric depot from the site of Benkov near Uničov, medieval silk textiles from Křížkovského Street in Olomouc or small objects of daily devotion in the modern period from the same research.</p>
<h5><strong>Sporting event:</strong></h5>
<p>Bowling in BOWLAND Bowling Center, Šantovka</p>


Advances in Chromatography and Electrophoresis & Chiranal 2022 - Programme

<p><strong>Symposium &quot;Advances in Chromatography and Electrophoresis &amp; Chiranal 2024&quot; belongs to the periodical meetings of specialists working in the field of separation methods. The biennial conference is organized by Department of Analytical Chemistry, Faculty of Science, Palacky University in cooperation with Czech Chemical Society and Czech Society for Mass Spectrometry. It is organized as a meeting of the scientists from the universities, academic institutes and experts from the routine analytical laboratories.</strong></p>
<p><a href="https://chiranal2024.upol.cz/index.php?link=programme"><strong>The scientific program</strong></a> will deal with all areas of separation science from fundamental to applied reseach in chemistry, physics, biology, medicine, forensic science and related disciplines. Official languages of the conference are English, Czech and Slovak.</p>
<h3><strong>Registration</strong></h3>
<ul>
<li><p><a href="https://chiranal2024.upol.cz/index.php?link=registration"><strong>Registration link</strong></a></p>
</li>
<li><p>Registration should be done <strong>until May 5th, 2024</strong>. </p>
</li>
<li><p>Registration <strong>fee is 5000,- CZK</strong> (200 EUR) + tax 21 %.</p>
</li>
</ul>
<p>Registration covers printed Program and Book of Abstracts, access to all scientific and social events held in the framework of the conference and meals.</p>
<h3><strong>Abstract submission</strong></h3>
<p><strong>Abstract submission deadline is May 5th, 2024.</strong></p>
<ul>
<li><p>Structured form of abstract is preferred. To prepare the abstract follow the form of the <a href="https://chiranal2024.upol.cz/index.php?link=abstract"><strong>downloadable template</strong></a>.</p>
</li>
<li><p>The abstract length is limited to 2 pages. Abstract can contain one figure (in .tiff format included in the abstract body) and/or one table (also included).</p>
</li>
<li><p>The abstracts included in Book of Abstracts have to be prepared in English.</p>
</li>
<li><p>Each participant will present only one oral or poster presentation.</p>
</li>
<li><p>Organizers reserve their right to change oral presentation to poster and vice versa.</p>
</li>
</ul>
<h5><strong>Lectures</strong></h5>
<ul>
<li>Time scheduled for invited lectures is <strong>40 minutes</strong>, for oral presentations is <strong>20 minutes</strong> (including discussion).</li>
</ul>
<h5><strong>Poster presentation</strong></h5>
<ul>
<li>Maximal poster height: <strong>180 cm</strong> and width: <strong>95 cm</strong> should be used. Posters have to be mounted using equipment provided by the organizers. (Do not use pins to mount the posters!).</li>
</ul>
<h3><strong>Conference site</strong></h3>
<h5><strong>Address of conference site:</strong></h5>
<p>Faculty of Science<br/>Palacky University<br/>17. listopadu 1192/12<br/>779 00 Olomouc</p>
<h5><strong>Registration:</strong></h5>
<ul>
<li><strong>Monday:</strong> Fort Science (before the plenary lecture).</li>
<li><strong>Tuesday:</strong> Faculty of Science, foyer, 2. floor.</li>
</ul>
<h5><strong>How to get there:</strong></h5>
<ol>
<li><p>From Train Station: Use any tram in the direction Nová Ulice or Neředín and get off at the second stop &quot;Envelopa&quot;.</p>
</li>
<li><p>From Bus Station: Use tram No. 4 (direction Nová Ulice) and get off at the fourth stop &quot;Envelopa&quot;.</p>
</li>
</ol>
<h3><strong>Programme</strong></h3>
<ul>
<li>Programme of the conference is in preparation.</li>
</ul>
<h3><strong>Accommodation</strong></h3>
<p>List of recommended hotels in Olomouc:</p>
<ul>
<li><p><a href="https://www.hotel-alley.com/">Hotel Alley</a></p>
</li>
<li><p><a href="https://www.hotelflora.cz/">Hotel Flora</a></p>
</li>
<li><p><a href="https://www.pensionujakuba.cz/">Pension U Jakuba</a></p>
</li>
<li><p><a href="https://www.orea.cz/hotel-arigone">Hotel Arigone</a> </p>
</li>
<li><p><a href="https://nhpension.eu/">Pension Na Hradbách</a></p>
</li>
<li><p><a href="https://www.lafayette.cz/english/default.htm">Hotel GarniHotel Lafayette</a></p>
</li>
</ul>
<h3><strong>Partners and Sponsors</strong></h3>
<h5><strong>Partners Chiranal 2024</strong></h5>
<ul>
<li><p><strong>Faculty of Science, Palacký University, Olomouc</strong></p>
</li>
<li><p><strong>Department of Analytical Chemistry, Palacký University, Olomouc</strong></p>
</li>
<li><p><strong>Czech Chemical Society</strong></p>
</li>
<li><p><strong>Chromatography &amp; Electrophores Czech Chemical Society</strong></p>
</li>
<li><p><a href="https://gcms.cz/companies/21"><strong>Czech Society for Mass Spectrometry</strong></a></p>
</li>
<li><p><strong>Fort Science</strong></p>
</li>
<li><p><strong>ARTECA</strong></p>
</li>
<li><p><strong>TEVA</strong></p>
</li>
<li><p><a href="https://lcms.cz/"><strong>LabRulezLCMS</strong></a></p>
</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Chiranal_2024_Partners_e3fc1710b0/Chiranal-2024-Partners.jpg" alt="Chiranal 2024: Partners"/></p>
<h5><strong>Sponsors Chiranal 2024</strong></h5>
<ul>
<li><p><a href="https://lcms.cz/companies/37"><strong>Altium Czech Republic</strong></a> / Agilent Technologies</p>
</li>
<li><p><a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a> / <strong>Thermo Scientific</strong></p>
</li>
<li><p><a href="https://lcms.cz/companies/176"><strong>Metrohm</strong></a></p>
</li>
<li><p><a href="https://lcms.cz/companies/43"><strong>SHIMADZU</strong></a></p>
</li>
<li><p><strong>AMEDIS</strong> / <strong>SCIEX</strong></p>
</li>
<li><p><a href="https://lcms.cz/companies/51"><strong>Phenomenex</strong></a></p>
</li>
<li><p><a href="https://lcms.cz/companies/50"><strong>Waters</strong></a></p>
</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Chiranal_2024_Sponsors_164d421196/Chiranal-2024-Sponsors.jpg" alt="Chiranal 2024: Sponsors"/></p>
<h3><strong>Permanent scientific committee</strong></h3>
<ul>
<li><p><strong>Daniel W. Armstrong (US)</strong></p>
</li>
<li><p><strong>Petr Barták (CZ)</strong></p>
</li>
<li><p><strong>Petr Bednář (CZ)</strong></p>
</li>
<li><p><strong>Salvatore Fanali (IT)</strong></p>
</li>
<li><p><strong>Bohuslav Gaš (CZ)</strong></p>
</li>
<li><p><strong>Václav Kašička (CZ)</strong></p>
</li>
<li><p><a href="https://lcms.cz/articles?search=karel+lemr"><strong>Karel Lemr (CZ)</strong></a></p>
</li>
<li><p><strong>Wolfgang Lindner (AT)</strong></p>
</li>
<li><p><strong>Eva Smolková (CZ)</strong></p>
</li>
<li><p><strong>Zdeněk Stránský (CZ)</strong></p>
</li>
<li><p><strong>Juraj Ševčík (CZ)</strong></p>
</li>
<li><p><a href="https://lcms.cz/articles?search=Franti%C5%A1ek+%C5%A0vec"><strong>František Švec (CZ)</strong></a></p>
</li>
<li><p><strong>Eva Tesařová (CZ)</strong></p>
</li>
</ul>
<h5><strong>Contacts</strong></h5>
<ul>
<li><p><strong>Dr. Ondřej Kurka</strong> (phone: +420 585 634 450, email: ondrej.kurka@upol.cz)</p>
</li>
<li><p><strong>Assoc. Prof. Petr Bednář</strong> (phone: +420 585 634 403, email: petr.bednar@upol.cz)</p>
</li>
</ul>


Advances in Chromatography and Electrophoresis & Chiranal 2024

<p><strong>The biennial conference is organized by Department of Analytical Chemistry, Faculty of Science, Palacky University in cooperation with Czech Chemical Society and Czech Society for Mass Spectrometry. It is organized as a meeting of the scientists from the universities, academic institutes and experts from the routine analytical laboratories.</strong></p>
<p>The scientific program will deal with all areas of separation science from fundamental to applied reseach in chemistry, physics, biology, medicine, forensic science and related disciplines. Official languages of the conference are English, Czech and Slovak.</p>
<h3><strong>Registration</strong></h3>
<blockquote>
<p><a href="https://chiranal2024.upol.cz/index.php?link=registration"><strong>Registration</strong></a></p>
</blockquote>
<p>Registration fee is 5000,- CZK (200 EUR) + tax 21 %.</p>
<p>Registration covers printed Program and Book of Abstracts, access to all scientific and social events held in the framework of the conference and meals.</p>
<p>The invoice should be paid until <strong>May 26th, 2024</strong>.</p>
<h3><strong>Programme</strong></h3>
<h3><strong>Monday 17th June</strong></h3>
<h5><strong>14:00 – 17:30 Registration</strong></h5>
<ul>
<li>Fort Science (foyer, ground floor), individual arrival of participants and accommodation</li>
</ul>
<h5><strong>16:00 – 16:15 Opening of the conference (Fort Science, Laudon Hall, ground floor), presence of official representatives of the conference partners</strong></h5>
<ul>
<li>Foreword: Petr Bednář</li>
</ul>
<h5><strong>16:15 – 17:15 Plenary Lecture</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<h5><strong>Toward unrivaled chromatographic resolving power: Design and development of comprehensive spatial three-dimensional liquid-phase separation technology</strong></h5>
<ul>
<li>Sebastiaan Eeltink</li>
</ul>
<h5><strong>17:30 – 17:45 Ceremonial launch of the book of Lucie Nováková, Michal Douša, Petr Česla and Jiří Urban: Modern HPLC separations in theory and practice</strong></h5>
<h5><strong>17:45 – ?? Welcome party + banquet</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UPOL_Chiranal_cover_image_6d8ebc4308/UPOL-Chiranal-cover-image.jpg" alt="Chiranal: Advances in Chromatography and Electrophoresis &amp; Chiranal 2024"/></p>
<h3><strong>Tuesday 18th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
<li>installation of posters (Faculty of Science, seminary rooms, 2nd floor)</li>
</ul>
<h4><strong>09:00 – 11:20 Lectures I: Electromigration techniques, ion exchange chromatography</strong></h4>
<ul>
<li>chairmen: Michael Lämmerhofer, Petr Bednář</li>
<li>Faculty of Science, assembly hall, 2nd floor)</li>
</ul>
<h5><strong>09:00 – 09:30 Electrokinetic methodologies for diagnostics development</strong></h5>
<ul>
<li>Anne Varenne</li>
</ul>
<h5><strong>09:30 – 10:00 Advances in microchip electrophoresis for the analysis of environmental and biological samples</strong></h5>
<ul>
<li>Marián Masár</li>
</ul>
<h5><strong>10:00 – 10:20 Determination of effective charge and ionic mobilities of highly sulfated cyclodextrins by capillary isotachophoresis and zone electrophoresis</strong></h5>
<ul>
<li>Václav Kašička</li>
</ul>
<h5><strong>10:20 – 10:40 Capillary Zone Electrophoresis – Mass spectrometry for intact protein analysis in biological fluids – possibilities and limitations</strong></h5>
<ul>
<li>Katarína Maráková</li>
</ul>
<h5><strong>10:40 – 11:00 Microcontrollers and their application in constructing analytical instrumentation</strong></h5>
<ul>
<li>Petr Kubáň</li>
</ul>
<h5><strong>11:00 – 11:20 Adsorbable organic fluorine (AOF) – a sum parameter for non-targeted screening of per- and polyfluorinated alkyl substances (PFASs) in waters</strong></h5>
<ul>
<li>Jan Soukup (<a href="https://lcms.cz/companies/176"><strong>Metrohm</strong></a>)</li>
</ul>
<h5><strong>11:20 – 11:40 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h4><strong>11:40 – 13:40 Lectures II: Approaches to method development in LC and MS, capillary chromatography</strong></h4>
<ul>
<li>chairmen: Anne Varenne, Petr Barták</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:40 – 12:10 How to successfully analyse oligonucleotides with liquid chromatography</strong></h5>
<ul>
<li>Davy Guillarme</li>
</ul>
<h5><strong>12:10 – 12:40 Recent applications of nano-liquid chromatography for the characterization of plant and food products</strong></h5>
<ul>
<li>Zeineb Aturki</li>
</ul>
<h5><strong>12:40 – 13:00 Personal insights on the design of experiments</strong></h5>
<ul>
<li>Jiří Urban</li>
</ul>
<h5><strong>13:00 – 13:20 How to make chromatography more sustainable</strong></h5>
<ul>
<li>Egidijus Machtejevas (Merck)</li>
</ul>
<h5><strong>13:20 – 13:40 Automated workflow for derivatized analyte analysis in LC-TIMS-MS/MS 4D-metabolomics data: Incorporating in-silico</strong></h5>
<ul>
<li>Aiko Barsch (Bruker Daltonics)</li>
</ul>
<h5><strong>13:40 – 14:40 Lunch</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>14:40 – 16:00 Poster session I (odd poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h4><strong>16:00 – 18:00 Lectures III: Current trends in chromatography</strong></h4>
<ul>
<li>chairmen: Václav Kašička, Ondřej Kurka</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>16:00 – 16:30 Isomer separations in metabolomics and lipidomics</strong></h5>
<ul>
<li>Michael Lämmerhofer</li>
</ul>
<h5><strong>16:30 – 17:00 New Trends in the Lipidomic and Metabolomic Analysis</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Michal+Hol%C4%8Dapek"><strong>Michal Holčapek</strong></a></li>
</ul>
<h5><strong>17:00 – 17:20 Unveiling the Power of Deep UV LAESI/LAESCI Dual Ambient Ionization: Towards Comprehensive Molecular MSI</strong></h5>
<ul>
<li>Barbora Papoušková</li>
</ul>
<h5><strong>17:20 – 17:40 Advanced small molecule mass spectrometry</strong></h5>
<ul>
<li>Luka Milivojevic (<a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a>/Thermo Fisher Scientific)</li>
</ul>
<h5><strong>17:40 – 18:00 Employing ballistic gradients, vacuum jacketed columns and prototype benchtop multi reflecting time-of-flight (MRT) to increase lipidomic analytical throughput whilst maintainig highly confident identifications</strong></h5>
<ul>
<li>Alex Muck (<a href="https://lcms.cz/companies/50"><strong>Waters</strong></a>)</li>
</ul>
<h5><strong>18:00 – 19:00 Dinner</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>19:15 – 21:00 Excursion to the Eureka University Brewery and Department of Analytical Chemistry</strong></h5>
<h3><strong>Wednesday 19th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h4><strong>09:00 – 11:20 Lectures IV: Advances in biomolecules analysis, heritage science</strong></h4>
<ul>
<li>chairmen: Sebastiaan Eeltink, Lukáš Kučera</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Advances in chiral separation of carboranes – new phenyl ring isosteres for drug development</strong></h5>
<ul>
<li>Radim Kučera</li>
</ul>
<h5><strong>09:30 – 10:00 Analysis of selected drugs in whole blood collected with a volumetric absorptive microsampling device</strong></h5>
<ul>
<li>Petra Štěrbová-Kovaříková</li>
</ul>
<h5><strong>10:00 – 10:20 Translation of omics into clinics</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=David+Friedeck%C3%BD"><strong>David Friedecký</strong></a></li>
</ul>
<h5><strong>10:20 – 10:40 Py/GC-MS characterization of oil paintings to correlate museum environment and materials condition over time for SMARTMUS-e project</strong></h5>
<ul>
<li>Paola Lucero</li>
</ul>
<h5><strong>10:40 – 11:00 Recent chromatographic advances in plant hormone profiling methods</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Ond%C5%99ej+Nov%C3%A1k"><strong>Ondřej Novák</strong></a> (on behalf of <a href="https://lcms.cz/companies/37"><strong>Altium</strong></a>)</li>
</ul>
<h5><strong>11:20 – 13:00 Poster session II (even poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h5><strong>13:00 – 14:00 Lunch</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>15:00 – 17:00 Social/sport event</strong></h5>
<ul>
<li>Bowling at Bowland Olomouc</li>
<li>A tour around Olomouc, visit of Regional Museum in Olomouc</li>
</ul>
<h5><strong>18:00 – ?? Social dinner</strong></h5>
<ul>
<li>Faculty of Science, foyer, 6th floor</li>
</ul>
<h3><strong>Thursday 20th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>09:00 – 11:00 Lectures V: Matrix effects, chiral separation, advances in stationary phases, supercritical fluid chromatography</strong></h5>
<ul>
<li>chairmen: Zeineb Aturki, Petr Fryčák</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Matrix effects in LC-MS: importance of correct calculation approach</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Lucie+Nov%C3%A1kov%C3%A1"><strong>Lucie Nováková</strong></a></li>
</ul>
<h5><strong>09:30 – 10:00 New trends in ion exchange-type chiral stationary phases and their chiral recognition mechanisms</strong></h5>
<ul>
<li><a href="https://lcms.cz/article/3991"><strong>Michal Kohout</strong></a></li>
</ul>
<h5><strong>10:00 – 10:20 Development of an easy-to-set-up multiple heart-cutting achiral-chiral LC-LC method for the analysis of branched-chain amino acids in food supplements</strong></h5>
<ul>
<li>Ghaid W.A. Abualzulof</li>
</ul>
<h5><strong>10:20 – 10:40 Rapid profiling of bioactive compounds using supercritical fluid chromatography coupled with tandem mass spectrometry</strong></h5>
<ul>
<li>Ivan Petřík</li>
</ul>
<h5><strong>10:40 – 11:00 Symbiosis of core-shell and fully porous particles: Implementing both for better HPLC and UHPLC results</strong></h5>
<ul>
<li>Jan Vlasák (<a href="https://lcms.cz/companies/51"><strong>Phenomenex</strong></a>)</li>
</ul>
<h5><strong>11:00 – 11:20 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>11:20 – 13:30 Lectures VI: Selectivity in chromatography and mass spectrometry and its optimization, analysis of natural products</strong></h5>
<ul>
<li>chairmen: Lucie Nováková, <a href="https://lcms.cz/articles?search=Tom%C3%A1%C5%A1+Pluh%C3%A1%C4%8Dek"><strong>Tomáš Pluháček</strong></a></li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:20 – 11:50 Targeted and untargeted analysis of psilocybin mushrooms using LC-MS</strong></h5>
<ul>
<li>Kevin Schug</li>
</ul>
<h5><strong>11:50 – 12:10 On the potential of two-dimensional liquid chromatography for environmental analysis</strong></h5>
<ul>
<li>Deirdre Cabooter</li>
</ul>
<h5><strong>12:10 – 12:30 Leveraging ion mobility spectrometry-mass spectrometry data using machine learning reveals provenance of indigo dyes</strong></h5>
<ul>
<li>Volodymyr Pauk</li>
</ul>
<h5><strong>12:30 – 12:50 Numerical optimization of gradient separations in RP and HILIC using non-linear retention models</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Petr+%C4%8Cesla"><strong>Petr Česla</strong></a></li>
</ul>
<h5><strong>12:50 – 13:10 Do we need separations in clinical diagnostics?</strong></h5>
<ul>
<li>Rutuja Patil</li>
</ul>
<h5><strong>13:10 – 13:30 Metabolomics analysis of Moringa oleifera leaves: correlation between in vitro effect on C2C12 myotubes cell line and geographical origin</strong></h5>
<ul>
<li>Mariateresa Maldini (Amedis)</li>
</ul>
<h5><strong>13:30 – 14:10 Closing of the conference and farewell drink (Faculty of Science, foyer, 2nd floor)</strong></h5>
<h5><strong>14:10 – 15:10 Lunch (university cafeteria)</strong></h5>
<h3><strong>Partners and Sponsors</strong></h3>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Chiranal_2024_Partners_e3fc1710b0/Chiranal-2024-Partners.jpg" alt="Chiranal 2024: Partners"/></p>
<ul>
<li><p><strong>Faculty of Science, Palacký University, Olomouc</strong></p>
</li>
<li><p><strong>Department of Analytical Chemistry, Palacký University, Olomouc</strong></p>
</li>
<li><p><strong>Czech Chemical Society</strong></p>
</li>
<li><p><strong>Chromatography &amp; Electrophores Czech Chemical Society</strong></p>
</li>
<li><p><a href="https://lcms.cz/companies/21"><strong>Czech Society for Mass Spectrometry</strong></a></p>
</li>
<li><p><strong>Fort Science</strong></p>
</li>
<li><p><strong>ARTECA</strong></p>
</li>
<li><p><strong>TEVA</strong></p>
</li>
<li><p><a href="https://lcms.labrulez.com/companies/1"><strong>LabRulezLCMS</strong></a></p>
</li>
</ul>
<h3><strong>Sponsors Chiranal 2024</strong></h3>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Chiranal_Sponsors_2024_14389caaaf.jpg" alt="Chiranal: Sponsors 2024"/></p>
<ul>
<li><a href="https://lcms.cz/companies/37"><strong>Altium International</strong></a></li>
<li><strong>Alven Laboratories</strong></li>
<li><strong>AMEDIS</strong>/<strong>SCIEX</strong></li>
<li><strong>Bruker</strong></li>
<li><a href="https://lcms.labrulez.com/companies/168"><strong>ECOM</strong></a></li>
<li><strong>Chromservis</strong></li>
<li><strong>MERCK</strong></li>
<li><a href="https://lcms.cz/companies/176"><strong>Metrohm</strong></a></li>
<li><a href="https://lcms.cz/companies/51"><strong>Phenomenex</strong></a></li>
<li><a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a>/<strong>Thermo Scientific</strong></li>
<li><a href="https://lcms.cz/companies/43"><strong>SHIMADZU</strong></a></li>
<li><strong>Villa Labeco</strong></li>
<li><a href="https://lcms.cz/companies/50"><strong>Waters</strong></a></li>
</ul>
<h3><strong>Permanent scientific committee</strong></h3>
<ul>
<li><p><strong>Daniel W. Armstrong (US)</strong></p>
</li>
<li><p><strong>Petr Barták (CZ)</strong></p>
</li>
<li><p><strong>Petr Bednář (CZ)</strong></p>
</li>
<li><p><strong>Salvatore Fanali (IT)</strong></p>
</li>
<li><p><strong>Bohuslav Gaš (CZ)</strong></p>
</li>
<li><p><strong>Václav Kašička (CZ)</strong></p>
</li>
<li><p><a href="https://lcms.cz/articles?search=Karel+Lemr"><strong>Karel Lemr (CZ)</strong></a></p>
</li>
<li><p><strong>Wolfgang Lindner (AT)</strong></p>
</li>
<li><p><strong>Eva Smolková (CZ)</strong></p>
</li>
<li><p><strong>Zdeněk Stránský (CZ)</strong></p>
</li>
<li><p><strong>Juraj Ševčík (CZ)</strong></p>
</li>
<li><p><a href="https://lcms.cz/articles?search=Franti%C5%A1ek+%C5%A0vec"><strong>František Švec (CZ)</strong></a></p>
</li>
<li><p><strong>Eva Tesařová (CZ)</strong></p>
</li>
</ul>
<h5><strong>Contacts</strong></h5>
<ul>
<li><p><strong>Dr. Ondřej Kurka</strong> (phone: +420 585 634 450, email: ondrej.kurka@upol.cz)</p>
</li>
<li><p><strong>Assoc. Prof. Petr Bednář</strong> (phone: +420 585 634 403, email: petr.bednar@upol.cz)</p>
</li>
</ul>


Advances in Chromatography and Electrophoresis & Chiranal 2024 (Programme)

<p><strong>The study presents a remote deep ultraviolet laser ablation inlet connected to a dual electrospray ionization–atmospheric pressure chemical ionization (rDUVLAESCI) source for molecular mass spectrometry. This system enables simultaneous spatial acquisition of MS data for diverse organic molecules, offering improved detection of both polar and nonpolar compounds. With a deep 193 nm UV laser, the system allows precise sampling from dried spots, supporting molecular MS imaging (MSI) at resolutions down to 3 μm.&nbsp;</strong></p><p>The rDUVLAESCI dual source provides complementary ionization modes, achieving high analyte sensitivity across a broad molecular range. Demonstrations include mapping psychoactive substances on latent fingerprints, linking chemical and biometric data for enhanced forensic analysis.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c04392"><strong>Remote Deep-Ultraviolet Laser Ablation in Connection with Electrospray Ionization–Atmospheric Pressure Chemical Ionization (rDUVLAESCI): A Novel Dual Ionization Source for Molecular Mass Spectrometry</strong></a></h4><p>Barbora Papoušková, Petr Fryčák, Filip Gregar, Karel Lemr, and Tomáš Pluháček</p><p>Anal. Chem. 2025, 97, 4, 2062–2069</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.analchem.4c04392">https://doi.org/10.1021/acs.analchem.4c04392</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p>Ambient mass spectrometry (MS) has become a powerful tool for analyzing a wide variety of molecules under native conditions. <a href="javascript:void(0);" aria-label="reference 1">(1)</a> One of the most advanced ambient techniques is represented by laser ablation/desorption followed by the post-ionization of desorbed neutral particles by anionization source, namely, electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photoionization (APPI). <a href="javascript:void(0);" aria-label="reference 2">(2)</a> During the last two decades, considerable efforts have been devoted to the development of advanced laser ablation electrospray ionization (LAESI) source designs. Lasers with wavelengths ranging from the deep UV (DUV) to the far IR have been successfully coupled with ESI, APCI, APPI, and low-temperature plasma ionization (LTP) to ionize various molecules with significantly different polarity and molecular weight. <a href="javascript:void(0);" aria-label="reference 2 to 4">(2−4)</a></p><p>In addition, UV and DUV laser ablation have attracted attention for their ability to focus the laser beam to small spots (low μm scale) and for the efficient absorption of short wavelengths by a wide range of molecules or sample surfaces, respectively. The efficient desorption/ablation of minute samples within a single laser pulse without any extensive analyte fragmentation <a href="javascript:void(0);" aria-label="reference 5 to 7">(5−7)</a> has allowed the DUV-LAESI/APPI/LTP MS analysis/visualization of intact small molecules, metabolites, <a href="javascript:void(0);" aria-label="reference 8 to 12">(8−12)</a> lipids, <a href="javascript:void(0);" aria-label="reference 5, 10 and 12">(5,10,12)</a> dyes/inks, <a href="javascript:void(0);" aria-label="reference 9">(9)</a> or even proteins <a href="javascript:void(0);" aria-label="reference 13 to 17">(13−17)</a> in various matrices ranging from model wet or dried spots <a href="javascript:void(0);" aria-label="reference 8, 11, 13, 14 and 16">(8,11,13,14,16)</a> and banknotes <a href="javascript:void(0);" aria-label="reference 9">(9)</a> to biological tissue sections. <a href="javascript:void(0);" aria-label="reference 5, 7, 10, 12, 14, 15 and 17">(5,7,10,12,14,15,17)</a> To the best of our knowledge, there has been no publication describing universal ion source design simultaneously acquiring DUVLAESI and DUVLAAPCI signals within a single analytical run with a variable laser spot size at the μm scale for direct analysis as well as for imaging purposes.</p><p>This study is aimed at the development and characterization of a novel dual rDUVLAESCI ion source that facilitates simultaneous electrospray and chemical ionization. Its applicability covers comprehensive direct analysis as well as MS imaging of a plethora of organic molecules. The source features a new custom-built interface and enables the automatic acquisition of rDUVLAESCI (ESI and APCI modes) data in a single analytical run. The simultaneous dual-mode acquisition facilitates new possibilities for the straightforward analysis of complex samples containing analytes of widely varying polarity. Analytes ranging from polar organic molecules to aromatic hydrocarbons were effectively ionized. Moreover, the proposed design with a remote laser ablation chamber provides an excellent lateral resolution of 3–160 μm and analytical performance allowing the analysis of minute samples. MSI of the traces of new psychoactive substances (naphyrone, butylone, cathinone, and flephedrone) visualized latent fingerprints linking illicit drug abuse with the identification of individuals.</p><h3><strong>Experimental</strong></h3><h4><strong>rDUVLAESCI Ion Source Setup</strong></h4><p>The full description of the developed rDUVLAESCI simultaneous dual acquisition source is presented in the <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.4c04392/suppl_file/ac4c04392_si_001.pdf">Supporting Information</a>. Briefly, an Analyte G2 remote UV laser ablation unit (Photon Machines, USA) with a 193 nm excimer nanosecond laser was connected to a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?search=synapt+g2"><strong>Synapt G2-S hybrid Q-TOF mass spectrometer (Waters Corporation, UK)</strong></a> via a proprietary interface designed for the commercially available ESCI Multimode ionization source (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c04392#fig1" data-tab="pane-pcw-Figures" id="">Figure 1</a>). The optimized parameters for rDUVLAESCI–MS and rDUVLAESCI–MSI automated data acquisition within a single analytical run are summarized in the <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.4c04392/suppl_file/ac4c04392_si_001.pdf">Supporting Information</a>. The mass spectra collected from the spot analyses were evaluated using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?search=masslynx"><strong>MassLynx 4.1 software (Waters Corporation, UK)</strong></a>. Extracted time-resolved data from imaging experiments were visualized using the ILAPS software originally developed for laser ablation data reduction and imaging applications. <a href="javascript:void(0);" aria-label="reference 18">(18)</a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2025_97_4_2062_2069_Figure_1_The_r_DUVLAESCI_interface_is_mounted_in_the_Waters_ESCI_Multimode_ionization_source_The_cloud_of_ablated_material_is_transported_orthogonally_toward_the_MS_inlet_into_523539839d.jpeg" alt="Anal. Chem. 2025, 97, 4, 2062–2069 - Figure 1. The rDUVLAESCI interface is mounted in the Waters ESCI Multimode ionization source. The cloud of ablated material is transported orthogonally toward the MS inlet into the ESI/APCI source" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2025_97_4_2062_2069_Figure_1_The_r_DUVLAESCI_interface_is_mounted_in_the_Waters_ESCI_Multimode_ionization_source_The_cloud_of_ablated_material_is_transported_orthogonally_toward_the_MS_inlet_into_523539839d.jpeg 224w," sizes="100vw" width="918" height="640"></p><h4><strong>Preparation of Standards for Spot Analysis</strong></h4><p>A set of reference analytes differing in polarity and molecular weight was used to characterize the ionization properties. For each analyte, the stock standard solution of 1 mg mL–1 was prepared in either heptane (anthracene), isopropanol (butylone, cathinone, flephedrone, naphyrone, ethyl stearate, squalene, stearic acid, and PEG 600), acetone (stearyl stearate), or methanol (palmitic acid), based on solubility. A 2 μL aliquot was pipetted onto the microscopic slide and left to dry at laboratory temperature to create a spot with an average diameter of 14 mm.</p><h4><strong>Preparation of Latent Fingerprints with Traces of New Psychoactive Substances</strong></h4><p>The two model latent fingerprints with trace amounts of new psychoactive substances were prepared to demonstrate the applicability of rDUVLAESCI–MS in molecular MSI. The following 5-step procedure was used to prepare minute volume samples: application of 50 μL of butylone and naphyrone (1 mg mL–1) on the microscopic slide (2.5 × 5 cm); spreading over the desired area; the complete drying of the slide at laboratory temperature; touching the microscope glass surface with a thumb; and a collection of latent fingerprints by printing on a new clean microscope glass. The second latent fingerprint sample with flephedrone and cathinone was prepared by using the same procedure with two donors placing their thumbs on the same microscopic slide to create partially overlapping fingerprints. The prepared latent fingerprints were subjected to MSI of endogenous and exogenous analytes using rDUVLAESCI–MSI. Personal identification was based on comparing characteristic dactyloscopic features of the drug and sebum-based 2D maps with the reference fingerprint visualized by a metallic dactyloscopic powder (bronze).</p><h3><strong>Results and Discussion</strong></h3><h4><strong>Visualization of New Psychoactive Substances on Latent Fingerprints Followed by Personal Identification</strong></h4><p>The rDUVLAESCI–MSI was probed on latent fingerprints collected from donors after direct contact with either dried flephedrone and cathinone or naphyrone and butylone. The simultaneous rDUVLAESCI–MSI acquisition allowed us to visualize the spatial distribution of new psychoactive substances adhered to papillary lines (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c04392#fig6" data-tab="pane-pcw-Figures" id="">Figure 6</a>), even in the case of the partially overlapped latent fingerprints (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c04392#fig7" data-tab="pane-pcw-Figures" id="">Figure 7</a>). The proper optimization of the laser frequency (20 Hz), helium gas flow rate (0.65 L min<sup>–1</sup>), and synchronization with MS scanning frequency (acquisition of 2 spectra per pixel) enables the reconstruction of the 2D images showing reliable and non-affected papillary line patterns with negligible carryover (no sebum-related signal detected between papillary lines). Therefore, the sebum-related compounds and the new psychoactive substances (wet fingerprint) highlighted the characteristic papillary line profile that is essential for direct forensic personal identification. The reconstruction of the papillary line pattern was based on detecting the sebum constituents, namely, squalene, cholesterol, waxes, etc. (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c04392#fig5" data-tab="pane-pcw-Figures" id="">Figures 5</a> and <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c04392#fig7" data-tab="pane-pcw-Figures" id="">7</a>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2025_97_4_2062_2069_Figure_6_a5d6c4fbba.jpeg" alt="Anal. Chem. 2025, 97, 4, 2062–2069 - Figure 6. Molecular MSI of butylone and naphyrone adhered to a latent fingerprint collected from the male and female donors. rDUVLAESCI–MSI 2D images for butylone (top) and naphyrone (bottom)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2025_97_4_2062_2069_Figure_6_a5d6c4fbba.jpeg 245w," sizes="100vw" width="1272" height="740"></p><p>The palmitoyl oleate image revealed an overlapped papillary pattern of two donors, who underwent the standard forensic fingerprint matching approach. The comparison of the characteristic dactyloscopic features provided positive matching with a visualized reference fingerprint (at least ten dactyloscopic markers are required for personal identification in Czechia and Slovakia). Even in the case of the overlapped fingerprint belonging to the donors who came into contact with flephedrone, personal identification was achieved (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.4c04392#fig7" data-tab="pane-pcw-Figures" id="">Figure 7</a>). More than 15 characteristic features of overlapped fingerprints were detected. The simultaneous rDUVLAESCI–MSI renders the link between molecular features and biometric information.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2025_97_4_2062_2069_Figure_7_7718081f70.jpeg" alt="Anal. Chem. 2025, 97, 4, 2062–2069 - Figure 7. Dactyloscopic identification of the male and female donor visualizing constituents of fingerprints. 2D image: (A) co-reregistration of the cathinone and flephedrone with the fingerprint pattern; (B) palmitoyl oleate overlapped image of both persons, the thicker latent fingerprint was collected for the female donor; and (C) reference fingerprints visualized using a standard dactyloscopic approach. The positive dactyloscopic markers confirmed personal identification for both new psychoactive substances containing fingerprint donors." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2025_97_4_2062_2069_Figure_7_7718081f70.jpeg 132w," sizes="100vw" width="958" height="1135"></p><h3><strong>Conclusions</strong></h3><p>Our work has introduced a novel comprehensive ambient technique for complex analysis. The simultaneous acquisition of rDUVLAESCI mass spectra within a single analytical run enables the analysis of various molecules differing significantly in their polarity. The technique opens a new window in comprehensive molecular MS either for dried spot samples or for visualization of the spatial distribution of analytes. The fully automated rDUVLAESCI–MSI provided a reliable image of the molecular distribution with a variable spatial resolution in the range from 160 to 3 μm. 2D images visualizing new psychoactive substances on latent fingerprints were used for direct personal identification, even in the case of overlapping fingerprints. The successful dactyloscopic identification confirms the image quality robustness for forensic applications.</p>

Remote Deep-Ultraviolet Laser Ablation in Connection with Electrospray Ionization–Atmospheric Pressure Chemical Ionization (rDUVLAESCI): A Novel Dual Ionization Source for Molecular Mass Spectrometry

<p><strong>Symposium "Advances in Chromatography and Electrophoresis &amp; Chiranal" belongs to the periodical meetings of specialists working in the field of separation methods. The biennial conference is organized by Department of Analytical Chemistry, Faculty of Science, Palacky University in cooperation with Czech Chemical Society and Czech Society for Mass Spectrometry. It is organized as a meeting of the scientists from the universities, academic institutes and experts from the routine analytical laboratories.</strong></p><p><a target="_blank" rel="noopener noreferrer" href="https://www.upol.cz/chiranal/programme/"><strong>The scientific program</strong></a> will deal with all areas of separation science from fundamental to applied reseach in chemistry, physics, biology, medicine, forensic science and related disciplines. Official languages of the conference are English, Czech and Slovak.</p><ul><li><strong>Date: </strong>15. - 18. June 2026</li><li><strong>Place:</strong> Olomouc, Czech Republic</li><li><strong>Contact: </strong>prof. RNDr. Petr Bednář, Ph.D. (Department of Analytical Chemistry, Faculty of Science, Palacky University)<ul><li><strong>e-mail: </strong>petr.bednar@upol.cz</li></ul></li></ul><h3><strong>Registration</strong></h3><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://www.upol.cz/chiranal/registration/"><strong>Register for the conference HERE</strong></a></p></blockquote><ul><li><strong>Conference Fees:</strong> 6.000,- CZK / 250 EUR &nbsp;without VAT</li><li><strong>Deadline for registration is May 31st, 2026.</strong></li><li>You will receive a registration confirmation by email.</li></ul><h3><strong>Abstract Submission</strong></h3><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://www.upol.cz/chiranal/abstract-submission/"><strong>Submit your Abstract HERE</strong></a></p></blockquote><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.upol.cz/fileadmin/users/381/template.docx"><strong>Download Abstract Template</strong></a></li><li>The <strong>deadline for submitting</strong> abstracts for <strong>oral presentations</strong> and posters is <strong>May 11, 2026</strong>.</li></ul><h3><strong>Preliminary Programme</strong></h3><h4><strong>Monday 15th June 2026, Fort Science</strong></h4><h5><strong>16:15 - 17:15 Plenary Lecture</strong></h5><ul><li>Michael Laemmerhofer</li></ul><h4><strong>Tuesday 16th June 2026</strong></h4><h5><strong>11:30 - 12:00 Enantioselective Ion Pairing Systems, my 50 years of Contribution</strong></h5><ul><li>Wolfgang Lindner&nbsp;</li></ul><h5><strong>16:30 - 17:00 Data science-assisted method development for on-line supercritical fluid extraction – chromatography</strong></h5><ul><li>Kevin Schug (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/301"><strong>The University of Texas at Arlington</strong></a>)</li></ul><h4><strong>Wednesday 17th June 2026</strong></h4><h5><strong>9:00 – 9:30 The Role of Analytical Chemistry in Solving Contemporary Challenges in Biomethane Plants</strong></h5><ul><li>Justyna Płotka-Wasylka</li></ul><h5><strong>11:30 - 12:00 Rational approaches to MALDI-MS matrix design</strong></h5><ul><li>Hans-Dieter Junker</li></ul><h5><strong>12:50 - 13:10 Using Charge Detection Mass Spectrometry with an Electrostatic Linear Ion Trap for the Analysis of Viral Vectors and Large Protein Complexes</strong></h5><ul><li>Alex Muck (Waters)</li></ul><h4><strong>Thursday 18th June 2026</strong></h4><h5><strong>9:00 – 9:30 Capillary Electrophoresis - Mass Spectrometry for the characterization of proteoforms on the intact level</strong></h5><ul><li>Christian Neusüß</li></ul><h5><strong>9:30 – 10:00 Investigation of acid-base and electromigration properties of biopeptides by capillary electrophoresis</strong></h5><ul><li>Václav Kašička</li></ul><h5><strong>11:30 - 12:00 Open-source capillary electrophoresis - democratizing separation science: Design principles and practical implementations from our laboratory</strong></h5><ul><li>Petr Kubáň</li></ul><h5><strong>12:00 - 12:30 Analysis of biomedical, environmental and pharmaceutical samples by microchip electrophoresis</strong></h5><ul><li>Marián Masár</li></ul><h5><strong>12:30 - 13:00 UV-LED coupling for high-sensitivity glycan analysis in commercial capillary electrophoresis system</strong></h5><ul><li>Jana Lavická</li></ul><h4><strong>Sponsors</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/companies/37"><strong>Altium International s.r.o. (HPST)</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/companies/50"><strong>Waters Gesellschaft m.b.H.</strong></a></li><li>Teva</li></ul><h4><strong>Partners</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/341"><strong>Department of Analytical chemistry, Palacký University Olomouc</strong></a></li><li>Faculty of Science, Palacký University Olomouc</li><li>SMALT (Spojené laboratoře pro materiálové a analytické technologie)</li><li>Czech society for mass spectrometry</li><li>Česká společnost chemická</li><li>ARTECA</li><li>Pevnost poznání</li><li>Odborná skupina chromatografie a elektroforézy ČSCH</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/1"><strong>LabRulez</strong></a></li></ul><h4><strong>Conference site</strong></h4><h5><strong>Address of conference site:</strong></h5><ul><li>Faculty of Science</li><li>Palacky University</li><li>17. listopadu 1192/12</li><li>779 00 Olomouc</li></ul>

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