<p><strong>The biennial conference is organized by Department of Analytical Chemistry, Faculty of Science, Palacky University in cooperation with Czech Chemical Society and Czech Society for Mass Spectrometry. It is organized as a meeting of the scientists from the universities, academic institutes and experts from the routine analytical laboratories.</strong></p>
<p>The scientific program will deal with all areas of separation science from fundamental to applied reseach in chemistry, physics, biology, medicine, forensic science and related disciplines. Official languages of the conference are English, Czech and Slovak.</p>
<h3><strong>Registration</strong></h3>
<blockquote>
<p><a href="https://chiranal2024.upol.cz/index.php?link=registration"><strong>Registration</strong></a></p>
</blockquote>
<p>Registration fee is 5000,- CZK (200 EUR) + tax 21 %.</p>
<p>Registration covers printed Program and Book of Abstracts, access to all scientific and social events held in the framework of the conference and meals.</p>
<p>The invoice should be paid until <strong>May 26th, 2024</strong>.</p>
<h3><strong>Programme</strong></h3>
<h3><strong>Monday 17th June</strong></h3>
<h5><strong>14:00 – 17:30 Registration</strong></h5>
<ul>
<li>Fort Science (foyer, ground floor), individual arrival of participants and accommodation</li>
</ul>
<h5><strong>16:00 – 16:15 Opening of the conference (Fort Science, Laudon Hall, ground floor), presence of official representatives of the conference partners</strong></h5>
<ul>
<li>Foreword: Petr Bednář</li>
</ul>
<h5><strong>16:15 – 17:15 Plenary Lecture</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<h5><strong>Toward unrivaled chromatographic resolving power: Design and development of comprehensive spatial three-dimensional liquid-phase separation technology</strong></h5>
<ul>
<li>Sebastiaan Eeltink</li>
</ul>
<h5><strong>17:30 – 17:45 Ceremonial launch of the book of Lucie Nováková, Michal Douša, Petr Česla and Jiří Urban: Modern HPLC separations in theory and practice</strong></h5>
<h5><strong>17:45 – ?? Welcome party + banquet</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UPOL_Chiranal_cover_image_6d8ebc4308/UPOL-Chiranal-cover-image.jpg" alt="Chiranal: Advances in Chromatography and Electrophoresis &amp; Chiranal 2024"/></p>
<h3><strong>Tuesday 18th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
<li>installation of posters (Faculty of Science, seminary rooms, 2nd floor)</li>
</ul>
<h4><strong>09:00 – 11:20 Lectures I: Electromigration techniques, ion exchange chromatography</strong></h4>
<ul>
<li>chairmen: Michael Lämmerhofer, Petr Bednář</li>
<li>Faculty of Science, assembly hall, 2nd floor)</li>
</ul>
<h5><strong>09:00 – 09:30 Electrokinetic methodologies for diagnostics development</strong></h5>
<ul>
<li>Anne Varenne</li>
</ul>
<h5><strong>09:30 – 10:00 Advances in microchip electrophoresis for the analysis of environmental and biological samples</strong></h5>
<ul>
<li>Marián Masár</li>
</ul>
<h5><strong>10:00 – 10:20 Determination of effective charge and ionic mobilities of highly sulfated cyclodextrins by capillary isotachophoresis and zone electrophoresis</strong></h5>
<ul>
<li>Václav Kašička</li>
</ul>
<h5><strong>10:20 – 10:40 Capillary Zone Electrophoresis – Mass spectrometry for intact protein analysis in biological fluids – possibilities and limitations</strong></h5>
<ul>
<li>Katarína Maráková</li>
</ul>
<h5><strong>10:40 – 11:00 Microcontrollers and their application in constructing analytical instrumentation</strong></h5>
<ul>
<li>Petr Kubáň</li>
</ul>
<h5><strong>11:00 – 11:20 Adsorbable organic fluorine (AOF) – a sum parameter for non-targeted screening of per- and polyfluorinated alkyl substances (PFASs) in waters</strong></h5>
<ul>
<li>Jan Soukup (<a href="https://lcms.cz/companies/176"><strong>Metrohm</strong></a>)</li>
</ul>
<h5><strong>11:20 – 11:40 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h4><strong>11:40 – 13:40 Lectures II: Approaches to method development in LC and MS, capillary chromatography</strong></h4>
<ul>
<li>chairmen: Anne Varenne, Petr Barták</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:40 – 12:10 How to successfully analyse oligonucleotides with liquid chromatography</strong></h5>
<ul>
<li>Davy Guillarme</li>
</ul>
<h5><strong>12:10 – 12:40 Recent applications of nano-liquid chromatography for the characterization of plant and food products</strong></h5>
<ul>
<li>Zeineb Aturki</li>
</ul>
<h5><strong>12:40 – 13:00 Personal insights on the design of experiments</strong></h5>
<ul>
<li>Jiří Urban</li>
</ul>
<h5><strong>13:00 – 13:20 How to make chromatography more sustainable</strong></h5>
<ul>
<li>Egidijus Machtejevas (Merck)</li>
</ul>
<h5><strong>13:20 – 13:40 Automated workflow for derivatized analyte analysis in LC-TIMS-MS/MS 4D-metabolomics data: Incorporating in-silico</strong></h5>
<ul>
<li>Aiko Barsch (Bruker Daltonics)</li>
</ul>
<h5><strong>13:40 – 14:40 Lunch</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>14:40 – 16:00 Poster session I (odd poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h4><strong>16:00 – 18:00 Lectures III: Current trends in chromatography</strong></h4>
<ul>
<li>chairmen: Václav Kašička, Ondřej Kurka</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>16:00 – 16:30 Isomer separations in metabolomics and lipidomics</strong></h5>
<ul>
<li>Michael Lämmerhofer</li>
</ul>
<h5><strong>16:30 – 17:00 New Trends in the Lipidomic and Metabolomic Analysis</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Michal+Hol%C4%8Dapek"><strong>Michal Holčapek</strong></a></li>
</ul>
<h5><strong>17:00 – 17:20 Unveiling the Power of Deep UV LAESI/LAESCI Dual Ambient Ionization: Towards Comprehensive Molecular MSI</strong></h5>
<ul>
<li>Barbora Papoušková</li>
</ul>
<h5><strong>17:20 – 17:40 Advanced small molecule mass spectrometry</strong></h5>
<ul>
<li>Luka Milivojevic (<a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a>/Thermo Fisher Scientific)</li>
</ul>
<h5><strong>17:40 – 18:00 Employing ballistic gradients, vacuum jacketed columns and prototype benchtop multi reflecting time-of-flight (MRT) to increase lipidomic analytical throughput whilst maintainig highly confident identifications</strong></h5>
<ul>
<li>Alex Muck (<a href="https://lcms.cz/companies/50"><strong>Waters</strong></a>)</li>
</ul>
<h5><strong>18:00 – 19:00 Dinner</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>19:15 – 21:00 Excursion to the Eureka University Brewery and Department of Analytical Chemistry</strong></h5>
<h3><strong>Wednesday 19th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h4><strong>09:00 – 11:20 Lectures IV: Advances in biomolecules analysis, heritage science</strong></h4>
<ul>
<li>chairmen: Sebastiaan Eeltink, Lukáš Kučera</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Advances in chiral separation of carboranes – new phenyl ring isosteres for drug development</strong></h5>
<ul>
<li>Radim Kučera</li>
</ul>
<h5><strong>09:30 – 10:00 Analysis of selected drugs in whole blood collected with a volumetric absorptive microsampling device</strong></h5>
<ul>
<li>Petra Štěrbová-Kovaříková</li>
</ul>
<h5><strong>10:00 – 10:20 Translation of omics into clinics</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=David+Friedeck%C3%BD"><strong>David Friedecký</strong></a></li>
</ul>
<h5><strong>10:20 – 10:40 Py/GC-MS characterization of oil paintings to correlate museum environment and materials condition over time for SMARTMUS-e project</strong></h5>
<ul>
<li>Paola Lucero</li>
</ul>
<h5><strong>10:40 – 11:00 Recent chromatographic advances in plant hormone profiling methods</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Ond%C5%99ej+Nov%C3%A1k"><strong>Ondřej Novák</strong></a> (on behalf of <a href="https://lcms.cz/companies/37"><strong>Altium</strong></a>)</li>
</ul>
<h5><strong>11:20 – 13:00 Poster session II (even poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h5><strong>13:00 – 14:00 Lunch</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>15:00 – 17:00 Social/sport event</strong></h5>
<ul>
<li>Bowling at Bowland Olomouc</li>
<li>A tour around Olomouc, visit of Regional Museum in Olomouc</li>
</ul>
<h5><strong>18:00 – ?? Social dinner</strong></h5>
<ul>
<li>Faculty of Science, foyer, 6th floor</li>
</ul>
<h3><strong>Thursday 20th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>09:00 – 11:00 Lectures V: Matrix effects, chiral separation, advances in stationary phases, supercritical fluid chromatography</strong></h5>
<ul>
<li>chairmen: Zeineb Aturki, Petr Fryčák</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Matrix effects in LC-MS: importance of correct calculation approach</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Lucie+Nov%C3%A1kov%C3%A1"><strong>Lucie Nováková</strong></a></li>
</ul>
<h5><strong>09:30 – 10:00 New trends in ion exchange-type chiral stationary phases and their chiral recognition mechanisms</strong></h5>
<ul>
<li><a href="https://lcms.cz/article/3991"><strong>Michal Kohout</strong></a></li>
</ul>
<h5><strong>10:00 – 10:20 Development of an easy-to-set-up multiple heart-cutting achiral-chiral LC-LC method for the analysis of branched-chain amino acids in food supplements</strong></h5>
<ul>
<li>Ghaid W.A. Abualzulof</li>
</ul>
<h5><strong>10:20 – 10:40 Rapid profiling of bioactive compounds using supercritical fluid chromatography coupled with tandem mass spectrometry</strong></h5>
<ul>
<li>Ivan Petřík</li>
</ul>
<h5><strong>10:40 – 11:00 Symbiosis of core-shell and fully porous particles: Implementing both for better HPLC and UHPLC results</strong></h5>
<ul>
<li>Jan Vlasák (<a href="https://lcms.cz/companies/51"><strong>Phenomenex</strong></a>)</li>
</ul>
<h5><strong>11:00 – 11:20 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>11:20 – 13:30 Lectures VI: Selectivity in chromatography and mass spectrometry and its optimization, analysis of natural products</strong></h5>
<ul>
<li>chairmen: Lucie Nováková, <a href="https://lcms.cz/articles?search=Tom%C3%A1%C5%A1+Pluh%C3%A1%C4%8Dek"><strong>Tomáš Pluháček</strong></a></li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:20 – 11:50 Targeted and untargeted analysis of psilocybin mushrooms using LC-MS</strong></h5>
<ul>
<li>Kevin Schug</li>
</ul>
<h5><strong>11:50 – 12:10 On the potential of two-dimensional liquid chromatography for environmental analysis</strong></h5>
<ul>
<li>Deirdre Cabooter</li>
</ul>
<h5><strong>12:10 – 12:30 Leveraging ion mobility spectrometry-mass spectrometry data using machine learning reveals provenance of indigo dyes</strong></h5>
<ul>
<li>Volodymyr Pauk</li>
</ul>
<h5><strong>12:30 – 12:50 Numerical optimization of gradient separations in RP and HILIC using non-linear retention models</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Petr+%C4%8Cesla"><strong>Petr Česla</strong></a></li>
</ul>
<h5><strong>12:50 – 13:10 Do we need separations in clinical diagnostics?</strong></h5>
<ul>
<li>Rutuja Patil</li>
</ul>
<h5><strong>13:10 – 13:30 Metabolomics analysis of Moringa oleifera leaves: correlation between in vitro effect on C2C12 myotubes cell line and geographical origin</strong></h5>
<ul>
<li>Mariateresa Maldini (Amedis)</li>
</ul>
<h5><strong>13:30 – 14:10 Closing of the conference and farewell drink (Faculty of Science, foyer, 2nd floor)</strong></h5>
<h5><strong>14:10 – 15:10 Lunch (university cafeteria)</strong></h5>
<h3><strong>Partners and Sponsors</strong></h3>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Chiranal_2024_Partners_e3fc1710b0/Chiranal-2024-Partners.jpg" alt="Chiranal 2024: Partners"/></p>
<ul>
<li><p><strong>Faculty of Science, Palacký University, Olomouc</strong></p>
</li>
<li><p><strong>Department of Analytical Chemistry, Palacký University, Olomouc</strong></p>
</li>
<li><p><strong>Czech Chemical Society</strong></p>
</li>
<li><p><strong>Chromatography &amp; Electrophores Czech Chemical Society</strong></p>
</li>
<li><p><a href="https://lcms.cz/companies/21"><strong>Czech Society for Mass Spectrometry</strong></a></p>
</li>
<li><p><strong>Fort Science</strong></p>
</li>
<li><p><strong>ARTECA</strong></p>
</li>
<li><p><strong>TEVA</strong></p>
</li>
<li><p><a href="https://lcms.labrulez.com/companies/1"><strong>LabRulezLCMS</strong></a></p>
</li>
</ul>
<h3><strong>Sponsors Chiranal 2024</strong></h3>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Chiranal_Sponsors_2024_14389caaaf.jpg" alt="Chiranal: Sponsors 2024"/></p>
<ul>
<li><a href="https://lcms.cz/companies/37"><strong>Altium International</strong></a></li>
<li><strong>Alven Laboratories</strong></li>
<li><strong>AMEDIS</strong>/<strong>SCIEX</strong></li>
<li><strong>Bruker</strong></li>
<li><a href="https://lcms.labrulez.com/companies/168"><strong>ECOM</strong></a></li>
<li><strong>Chromservis</strong></li>
<li><strong>MERCK</strong></li>
<li><a href="https://lcms.cz/companies/176"><strong>Metrohm</strong></a></li>
<li><a href="https://lcms.cz/companies/51"><strong>Phenomenex</strong></a></li>
<li><a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a>/<strong>Thermo Scientific</strong></li>
<li><a href="https://lcms.cz/companies/43"><strong>SHIMADZU</strong></a></li>
<li><strong>Villa Labeco</strong></li>
<li><a href="https://lcms.cz/companies/50"><strong>Waters</strong></a></li>
</ul>
<h3><strong>Permanent scientific committee</strong></h3>
<ul>
<li><p><strong>Daniel W. Armstrong (US)</strong></p>
</li>
<li><p><strong>Petr Barták (CZ)</strong></p>
</li>
<li><p><strong>Petr Bednář (CZ)</strong></p>
</li>
<li><p><strong>Salvatore Fanali (IT)</strong></p>
</li>
<li><p><strong>Bohuslav Gaš (CZ)</strong></p>
</li>
<li><p><strong>Václav Kašička (CZ)</strong></p>
</li>
<li><p><a href="https://lcms.cz/articles?search=Karel+Lemr"><strong>Karel Lemr (CZ)</strong></a></p>
</li>
<li><p><strong>Wolfgang Lindner (AT)</strong></p>
</li>
<li><p><strong>Eva Smolková (CZ)</strong></p>
</li>
<li><p><strong>Zdeněk Stránský (CZ)</strong></p>
</li>
<li><p><strong>Juraj Ševčík (CZ)</strong></p>
</li>
<li><p><a href="https://lcms.cz/articles?search=Franti%C5%A1ek+%C5%A0vec"><strong>František Švec (CZ)</strong></a></p>
</li>
<li><p><strong>Eva Tesařová (CZ)</strong></p>
</li>
</ul>
<h5><strong>Contacts</strong></h5>
<ul>
<li><p><strong>Dr. Ondřej Kurka</strong> (phone: +420 585 634 450, email: ondrej.kurka@upol.cz)</p>
</li>
<li><p><strong>Assoc. Prof. Petr Bednář</strong> (phone: +420 585 634 403, email: petr.bednar@upol.cz)</p>
</li>
</ul>


Advances in Chromatography and Electrophoresis & Chiranal 2024 (Programme)

<h3><strong>Promotion Alert!</strong></h3>
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Synthesis and absolute configuration of cyclic synthetic cathinones derived from α-tetralone

<p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1001"><strong>The PDA Extension</strong></a><strong> is an optional Extension that is used for processing data that has been acquired from selected photo diode array detectors. Spectral data, together with chromatograms, add a third dimension to analytical data analysis. This extension expands the capability of Clarity by providing interactive spectral analysis, peak purity analysis and compound identification that is based on a spectral library search. PDA data can be displayed in a set of optional views including 3D rendering.</strong></p><p>All data is saved in a single file; a chromatogram at any wavelength or even only a spectrum can simply be recalled for review after an analysis. UV-VIS spectra, acquired using a PDA detector, may be interactively selected from a chromatogram signal for visual inspection and comparison. The spectra may also be used for peak purity determinations, wavelength optimization and component identification through spectral libraries.</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=58&amp;search=pda+extension"><strong>The PDA Extension</strong></a> is an optional, fully integrated addition to <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity software</strong></a>.&nbsp;</li><li>It can be ordered as a part of new software or as an extension to existing software.</li><li>The <strong>LC-PDA</strong> mode is selectable for any LC Instrument within a station.&nbsp;</li><li>The PDA Extension is also compatible with Clarity Offline software.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_PDA_Extension_776dbd48dd.jpg" alt="DataApex - PDA Extension" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_PDA_Extension_776dbd48dd.jpg 245w," sizes="100vw" width="1206" height="534"></p><h4><strong>Features</strong></h4><ul><li><strong>PDA Data:</strong> All data is saved in a single file; a chromatogram at any wavelength or a spectrum only can be simply recalled after an analysis for review. UV-VIS spectra, acquired using a PDA detector, may be interactively selected from a chromatogram signal for visual inspection and comparison. The spectra may also be used for peak purity determinations, wavelength optimization and component identification through spectral libraries.</li><li><strong>PDA View:</strong> The PDA view is customizable; up to four views can be displayed at one time (possible views: Isoplot, Chromatogram, Spectra, 3D Display, Peak Purity, Peak Purity Spectra, Library Spectra Search and Spectral Library). The user can easily extract chromatographic signals from PDA data to determine the optimal detection wavelength for each peak.</li><li><strong>PDA Method:</strong> The Clarity PDA method includes an option for Spectral Library Search and Peak Purity analysis.</li><li><strong>Peak Purity:</strong> This analysis helps to discover hidden impurities. Purity is calculated from 5 or all spectra within the peak. The similarity curve is displayed in the PDA window. Peak Purity analysis can be optimized by setting custom preferences relating to the purity threshold, wavelength restriction and absorbance threshold.</li><li><strong>Spectral Library:</strong> The Clarity software compares the peak spectra with the spectra of an unlimited number of spectral libraries. Spectra stored in a Spectral Library include retention times and analysis parameters. The Spectral Library Search can perform automatic identification of integrated/calibrated components (peaks). The library search may be constrained by the RT Window and by Wavelength Range. A Background Correction option is also available.</li><li><strong>Import/Export Data:</strong> Spectral data can be imported/exported in ASCII text formats to or from the Clarity software.</li><li><strong>Reports:</strong> Users can easily include PDA options such as data 3D Display, Isoplot, Spectra, Peak Purity and Library Search results in reports using the intuitive Report Setup dialog.</li><li><strong>Instrument Control:</strong> Control modules (p/n A24) for Agilent, Shimadzu, Knauer and Duratec DADs are available (as of May 2007). Additional control modules for other PDA detectors can be developed upon request.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_The_PDA_Window_displays_four_views_organized_in_splitters_8c614fb6f0.jpg" alt="DataApex: The PDA Window displays four views organized in splitters. Currently displayed in the example are: Isoplot, Chromatogram, Spectral, 3D Display The user can additionaly select also Peak Purity, Peak Purity Spectra, Library Spectra Search and Spectral Library." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_The_PDA_Window_displays_four_views_organized_in_splitters_8c614fb6f0.jpg 191w," sizes="100vw" width="1098" height="896"></p><h4><strong>Specification</strong></h4><ul><li><strong>Part No.:</strong> A29</li><li><strong>Related Products:</strong>&nbsp;<ul><li>Clarity (p/n C50) - required</li><li>Clarity offline (p/n C59)</li><li>LC Control (p/n A24) - see the list of available PDA detectors at www.dataapex.com</li></ul></li></ul>

Clarity software - PDA (Photodiode Array) Extension

<h3><strong>Introduction</strong></h3><p><strong>This document presents:</strong></p><ul><li>a comparison of integration algorithms (IAs) available in <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/999"><strong>Clarity</strong></a>,</li><li>a generally proposed workflow for integrating chromatograms,</li><li>description of functions that are available only in the <i>Wave </i>algorithm.</li></ul><p>Integration algorithms are a crucial component of any chromatography software. They identify peaks in a raw chromatographic curve, assign integration marks (starts, ends, apexes), and determine the peak baseline. Although it is the operator's responsibility to verify the correct peak integration after the analysis is finished, an automated peak detection facilitated by the integration algorithm can significantly streamline the workflow.</p><p>Clarity since version 9.0 offers two IAs: the original algorithm, renamed <i><strong>Legacy</strong></i>, and a newly introduced algorithm named <i><strong>Wave</strong></i>, which evolved from an experimental algorithm present since version 7.0. The <i>Wave </i>algorithm will be regularly updated with new functions, settings, and bug fixes (updates for <i>Legacy </i>will only be released if a critical bug is discovered). Changes in IAs are always versioned and distinguishable by records in the audit trail.</p><h3><strong>Choosing an integration algorithm</strong></h3><p>Users can select either the <i>Legacy </i>or <i>Wave </i>integration algorithm for peak detection. The IA drop-down selection has been relocated to the <i>Integration </i>tab in both the <i>Chromatogram </i>window and the <i>Method Setup</i> dialog, highlighting the interconnection between the algorithm and the integration table.</p><p>The introduction of <i>Wave </i>does not affect existing results or methods, as they will remain set to <i>Legacy </i>unless manually altered. Suppose a method was created using an older version of any integration algorithm and was not changed after the Clarity update to a newer version. In that case, the latest version of the same algorithm will be used in any newly measured chromatograms. This change will be logged in these chromatograms upon creation. To address potential issues, DataApex aims to use the algorithm version with the most bug fixes. Users are not required to re-save their methods, but if they do, the change will be logged in the method audit trail.</p><p>One way to apply an integration algorithm to multiple chromatograms simultaneously is through the <i>Batch </i>dialog (not applicable to<i> Clarity Lite</i>).</p><h3><strong>General Comparison</strong></h3><p>Neither algorithm can be universally deemed superior; some situations may warrant using one IA over the other. Below are some examples of performance differences between the two IAs. At least the<i> Global Peak Width </i>and <i>Global Threshold</i> operations were presumed to be performed on the signal. If you encounter any issues with either of the integration algorithms, please inform DataApex via <a>support@dataapex.com</a>. This datasheet further contains an overview of a basic integration workflow.</p><h4><strong>Advantages of Wave IA:</strong></h4><ul><li>Handling of negative spikes/peaks with regard to baseline placement.</li><li>Handling of shifting baseline/signal.</li><li>Separation of unresolved peaks.</li></ul><h4><strong>Disadvantages of Wave IA:</strong></h4><ul><li>More sensitive to noise, requiring the use of <i>Bunching </i>or another filter in some cases.</li><li>Incorrect integration when peaks of significantly different widths are present (typical for GPC or EA).</li></ul><h4><strong>Negative spikes/peaks</strong></h4><p>When a chromatogram contains significant negative peaks or spikes that should not be evaluated, the default integration in the <i>Wave </i>algorithm will produce satisfactory results, while the <i>Legacy </i>algorithm will not. (In <i>Legacy</i>, this problem could be solved by applying several Integration Intervals.)</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_4b4372d5b8.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 1. Integration by Legacy IA considers the negative spike to be the start of the peak (or peak cluster)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_4b4372d5b8.jpg 245w," sizes="100vw" width="1063" height="548"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_11_e51da3eb48.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 2. Integration by Wave IA with the same parameters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_11_e51da3eb48.jpg 245w," sizes="100vw" width="1061" height="554"></p><h4><strong>Shifting signal/baseline</strong></h4><p>Similar to the negative peaks/spikes, the same situation may arise with a shifting baseline where the peaks should be evaluated to that baseline. <i>Legacy </i>IA will provide unsatisfactory integration. In <i>Legacy</i>, this could be solved by applying<i> Baseline Valley </i>to several places and combining them with <i>Integration Interval</i>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_13_d44f34c303.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 3. Integration by Legacy IA." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_13_d44f34c303.jpg 245w," sizes="100vw" width="1064" height="551"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_14_8c3d2772fe.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 4. Integration by Wave IA with the same parameters. The fairly high number of peaks is caused by the low value of the Global Threshold." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_14_8c3d2772fe.jpg 245w," sizes="100vw" width="1062" height="553"></p><h4><strong>Unresolved Peaks</strong></h4><p>In <i>Legacy </i>IA, if a peak does not fulfill some criteria (it is too narrow or the valley to other non-resolved peaks is too low), it gets joined to a neighboring bigger peak. Meanwhile, <i>Wave </i>IA evaluates the valley depths much better and separates the peaks automatically, even when the small peak does not fulfill some of the criteria. Small impurities, thus, do not influence the area of the bigger peak of interest.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_10_49fded9390.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 5. Integration by Legacy IA." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_10_49fded9390.jpg 245w," sizes="100vw" width="1062" height="554"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_12_6d8d9a6980.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 6. Integration by Wave IA with the same parameters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_12_6d8d9a6980.jpg 245w," sizes="100vw" width="1062" height="551"></p><h4><strong>Significant noise</strong></h4><p>In some cases, the signal noise may be very significant, and the set threshold value in the <i>Wave </i>algorithm will be so low that one peak will be split into several peaks. The example below shows that neither of the IAs is sufficient with just <i>Global Peak Width</i> and <i>Global Threshold</i>; additional operations must be used.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_9_e020e43a49.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 7. Integration by Legacy IA with the same parameters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_9_e020e43a49.jpg 245w," sizes="100vw" width="1062" height="552"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_8_1b275412a8.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 8. Integration by Wave IA." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_8_1b275412a8.jpg 245w," sizes="100vw" width="1064" height="552"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_7_4071029dd3.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 9. Integration by Wave IA after applying Global Bunching. Sometimes, small peaks at the start/end of a bigger one may remain." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_7_4071029dd3.jpg 245w," sizes="100vw" width="1061" height="550"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_6_ee88c557f1.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 10. Integration by Wave IA after applying Global Bunching and Minimal Area parameters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_6_ee88c557f1.jpg 245w," sizes="100vw" width="1063" height="556"></p><h4><strong>Peaks with significantly different peak widths</strong></h4><p>Sometimes, chromatograms have very different peak widths; when some peaks cover a significant portion of the chromatogram (e.g., in EA or GPC), <i>Wave </i>IA may fail to evaluate the wide peaks properly. Currently, it is recommended to integrate these chromatograms using <i>Legacy </i>IA.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_3_b833975244.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 11. Integration by Legacy IA with the same parameters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_3_b833975244.jpg 245w," sizes="100vw" width="1060" height="549"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_4_660685f241.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 12. Integration by Wave IA." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_4_660685f241.jpg 245w," sizes="100vw" width="1060" height="551"></p><h4><strong>Peaks cut off in the middle</strong></h4><p>Sometimes, random peaks get integrated strangely in <i>Wave </i>IA, being cut off. This happens only randomly in the Wave IA (in a series of chromatograms, most peaks are OK, but occasional chromatograms may have one or more peaks cut off). The phenomenon results from filtering the data in combination with the signal curve course. There is no 100% solution in the <i>Wave </i>algorithm (applying <i>Global Bunching</i> or other filters helps in most cases). Switching to <i>Legacy </i>IA helps.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_2_ce68c8dc99.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 13. Integration by Wave IA." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_2_ce68c8dc99.jpg 245w," sizes="100vw" width="1064" height="553"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_5_af9302130f.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 14. Integration by Wave IA after applying Global Bunching." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_5_af9302130f.jpg 245w," sizes="100vw" width="1061" height="549"></p><h3><strong>Basic integration workflow</strong></h3><p>Setting at least the <i>Integration Interval</i> and global parameters before resorting to other operations is recommended. This strategy suffices for most chromatograms, and such integration is then applicable to all similar samples. The recommended order of operations is as follows (refer to the <i>User Guide</i> for a detailed description):</p><ol><li><strong>Set Integration Interval(s)</strong><br>Some areas of the chromatograms are irrelevant to the analysis and may cause wrong integration of the rest of the chromatogram if left in operation, e.g., sudden steep signal rises/drops when lamps are switched on, mobile phase compositions suddenly changing, etc. Make sure that the <i>Integration Interval </i>operation does not define an interval starting or ending inside of a peak slope; in either IA, the expectation is that the chromatogram starts and ends on a baseline.</li><li><strong>Set Global Peak Width</strong><br>If the chromatogram has peaks with significantly differing peak widths, applying the <i>Local Peak Width</i> operation may be useful later.</li><li><strong>Use Global Bunching operation</strong><br><i>Global Bunching</i> is calculated based on the chromatogram frequency and the set <i>Global Peak Width</i>, so it should be applied after setting the <i>Global Peak Width</i>.</li><li><strong>Set Global Threshold</strong><br>Based on the <i>Threshold </i>value, the smallest peaks are omitted depending on the noise of the signal curve. Since <i>Global Bunching</i> acts as a signal filter, the automatically suggested<i> Global Threshold </i>value will differ after applying <i>Global Bunching</i> higher than 1.</li></ol><h3><strong>Exclusive features in Wave IA</strong></h3><p><i>Wave </i>IA contains some Integration Table functions that are unavailable in <i>Legacy </i>IA. If these functions are present in the <i>Integration Table</i>, they will become invisible (not performed) when switching to the <i>Legacy </i>IA but will remain if the algorithm reverts to <i>Wave</i>.</p><h4><strong>Peak – Show and Peak – Hide</strong></h4><p>The <i>Peak </i>– <i>Show </i>and <i>Peak </i>– Hide functions provide a convenient way to manage the visibility of specific peaks in your data. These tools allow you to tailor your display by either showing or hiding specific peaks.</p><p>By default, the <i>Wave </i>algorithm might automatically ignore some of the minor peaks identified during integration. However, completely eliminating these peaks could potentially affect the baseline's trajectory. Therefore, these functions offer a flexible way to handle unwanted peaks without completely removing them.</p><p><i>Both </i>the <i>Peak </i>– <i>Show </i>and <i>Peak </i>– Hide features are complemented by the <i>Baseline </i>– <i>Lock </i>function. This tool completely removes all peaks that have their maxima within a set time interval. This could affect other peaks with maxima outside the specified window, particularly if they share the same baseline.</p><p>The <i>Peak </i>– Hide function has been designed within the <i>Wave </i>algorithm to address this. It merely hides peaks within the selected time interval instead of eradicating them. These peaks are considered when constructing a joint baseline but excluded from the final calculation and the <i>Result Table</i>. The counterpart function <i>Peak </i>- Show allows you to visualize a previously hidden peak or display peaks automatically hidden by the algorithm.</p><p>In scenarios where a region of the chromatogram contains hidden peaks and the user executes the <i>Peak </i>– <i>Add Positive/Negative </i>operation, all automatically generated but concealed peaks within the defined time interval are replaced by the newly added peak. This could lead to the cancellation of hidden peaks in areas where the manually added peak is not displayed due to baseline crossing limitations.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_Algorithms_in_Clarity_15_b934973f35.jpg" alt="DataApex: Integration Algorithms in Clarity: Figure 15. Difference between Peak Hide (left) and Baseline Lock (right)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Integration_Algorithms_in_Clarity_15_b934973f35.jpg 245w," sizes="100vw" width="1830" height="285"></p><h4><strong>Global/Local Baseline Slope</strong></h4><p>Automatic peak generation on a standard signal curve typically comprises three parts: Peak Start, Peak Apex, and Peak End. The highest signal point determines the location of Peak Apex. At the same time, the positioning of Peak Starts and Peak Ends can vary, potentially being either closer or farther from the Peak Apex, based on user preference. Different settings can be more effective depending on the situation.</p><p>Before the introduction of the Wave IA, the automatic determination of Peak Starts and Peak Ends was governed by the instantaneous peak slope, calculated using the Global/Local Threshold parameter. If users wanted the automatically generated peak borders closer to the Peak Apex, they had to increase the Threshold value. However, this resulted in raising the detection limit for peaks on the baseline, which could prevent the identification of smaller peaks.</p><p><i>Wave </i>IA presents an alternative. It allows users to maintain their chosen Threshold value while separately defining the chromatographic curve slope for detecting Peak Starts and Peak Ends through the use of the Global Baseline Slope or Local Baseline Slope operations. This parameter is not mandatory; users can retain the original slope calculation method by leaving the Global Baseline Slope value set to 0. Any value other than zero will cause a shift in the positions of the Peak Starts and Peak Ends.</p><p>You can find an approximate value for this function using the Chromatogram – Show Slope/Level function in the Chromatogram window menu. However, altering the Baseline Slope value may result in separating peak clusters into individual peaks or merging separate peaks, and some peaks may not be integrated at all.</p><p>For specific alterations to the start and/or end positions of individual peaks, the Peak – Start and Peak – End operations remain useful. These functions override the automatically calculated positions for peak start and peak end.</p>

Integration Algorithms in Clarity

<p><strong>Clarity 10.1 expands calibration capabilities to help you work more confidently and efficiently. From clearer guidance and better warnings to flexible internal standard evaluation, this update streamlines calibration development, improves traceability, and supports compliance where it matters most.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_10_1_397aba9bab.jpg" alt="DataApex: Clarity 10.1 released" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Clarity_10_1_397aba9bab.jpg 143w," sizes="100vw" width="849" height="925"></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/P063_Clarity_10_1_New_Features_283323fcd2.pdf"><strong>Clarity 10.1 highlights</strong></a></h4><ul><li>ISTD from a different signal</li><li>Determination Coefficient (R<sup>2</sup>)</li><li>Enhanced calibration warnings and blank handling</li><li>Electronic Signature improvements</li><li>New and updated control modules</li></ul><h5><strong>Internal standard from another signal</strong></h5><p>Clarity 10.1 introduces support for <strong>evaluating results</strong> using an internal standard <strong>from a different signal</strong> within the same chromatogram. This is especially useful for workflows like <strong>ICP-MS</strong>, where some signals may lack an ISTD peak.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Internal_standard_from_another_signal_2f2ac068f8.jpg" alt="DataApex: Internal standard from another signal" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Internal_standard_from_another_signal_2f2ac068f8.jpg 228w," sizes="100vw" width="380" height="260"></p><h5><strong>Determination Coefficient (R²)</strong></h5><p>Clarity now displays the <strong>Determination Coefficient (R²)</strong> alongside the Correlation Coefficient in each compound's calibration curve tab.</p><p>Determination Coefficient (R²) gives you a more accurate view of how well your calibration model fits the data - especially valuable during method development and validation.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Determination_Coefficient_57673d0ac3.jpg" alt="DataApex: Determination Coefficient" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Determination_Coefficient_57673d0ac3.jpg 228w," sizes="100vw" width="380" height="260"></p><h5><strong>Enhanced calibration warnings and blank handling</strong></h5><p>Clarity 10.1 helps you catch issues earlier and set up calibration with greater confidence. Warnings are now more visible and more specific - appearing above both the <strong>Results Table</strong> and the <strong>Calibration Summary Table</strong>. When multiple issues occur, an ellipsis appears with full details available on hover.</p><p>Blank handling has also been clarified: blanks are included only when <strong>Ignore Origin</strong> is selected. If the origin is used, blanks are excluded, and Clarity now clearly warns and visually shows when a blank is ignored.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Dataapex_Enhanced_calibration_warnings_and_blank_handling_e9fe7fa31e.jpg" alt="Dataapex: Enhanced calibration warnings and blank handling" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Dataapex_Enhanced_calibration_warnings_and_blank_handling_e9fe7fa31e.jpg 228w," sizes="100vw" width="380" height="260"></p><h5><strong>Signature improvements</strong></h5><p>Clarity 10.1 improves how signatures are handled, making it easier to understand what is and isn't signed. Chromatograms opened with a linked calibration are always marked <strong>Not Signed</strong>. Once signed, the mode switches to <strong>Stored Calibration</strong>, so you always see the final, signed result. Even historical versions now reliably show their signature state and details.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Dataapex_Signature_improvements_a2bebe7875.jpg" alt="Dataapex: Signature improvements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Dataapex_Signature_improvements_a2bebe7875.jpg 228w," sizes="100vw" width="380" height="260"></p><blockquote><p>Explore the complete list of all changes, fixes, and additions in version 10.1.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1"><strong>See the complete changelog</strong></a></p></blockquote>

Clarity Chromatography Software 10.1 released!

<p><strong>Clarity's Dual Chromatogram Mode is a specialized configuration designed for analytical systems that require simultaneous acquisition and processing of data from multiple detectors. Most commonly applied in dual tower gas chromatograph (GC) systems, this mode allows for the real-time acquisition of two chromatograms - each representing distinct detector outputs.</strong></p><p>This guide provides an in-depth look into the configuration, operational mechanics, and benefits of using Dual Chromatogram Mode within <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/999"><strong>Clarity software</strong></a> by DataApex.</p><h4><strong>Tools and System Requirements</strong></h4><h5><strong>Prerequisites</strong></h5><ul><li><strong>Clarity Chromatography Software</strong></li><li><strong>Dual Detector System</strong> (e.g., Dual Tower GC)</li><li>Properly configured <strong>System Configuration files</strong></li><li><strong>Signal Channels</strong> connected to respective detectors</li></ul><h4><strong>System Configuration and Activation</strong></h4><h5><strong>Enabling Dual Chromatogram Mode</strong></h5><p>To enable this mode, the user must access the <strong>System Configuration</strong> settings and individually activate Dual Chromatogram Mode for each instrument. This configuration determines how signal inputs from multiple detectors are routed.</p><h5><strong>Assigning Signals</strong></h5><p>Once activated:</p><ul><li>Signals are split and <strong>assigned</strong> to either the <strong>Front</strong> or <strong>Back</strong> chromatogram.</li><li>This signal labeling is consistent throughout the Clarity software workflow, ensuring accurate data mapping and separation.</li></ul><h4><strong>Method Setup and Calibration</strong></h4><h5><strong>Method Adaptation</strong></h5><p>Each method needs to be adapted to work with Dual Chromatogram Mode:</p><ul><li>Signals are distinctly labeled by position (Front/Back).</li><li>Each method tab includes <strong>separate configuration areas</strong> for each signal path.</li></ul><h5><strong>Calibration Files</strong></h5><ul><li>If calibration is used, <strong>individual calibration files</strong> must be assigned to the front and back chromatogram paths within the method settings.</li></ul><h4><strong>Interface and Workflow Adjustments</strong></h4><h5><strong>Chromatogram and Instrument Windows</strong></h5><ul><li>The <strong>Instrument Window</strong> is visually adapted to display both signal positions side by side.</li><li>The <strong>Chromatogram Window</strong> clearly labels which detector the chromatogram originated from by indicating position in the <strong>Measurement Conditions</strong> section.</li></ul><h5><strong>Sequence Window Enhancements</strong></h5><ul><li>Identification columns in the <strong>Sequence Window</strong> are designated separately for each chromatogram.</li><li>Other columns (like sample names, batch parameters) are shared and applied to both positions.</li></ul><h4><strong>Reporting and Data Processing</strong></h4><h5><strong>Report Customization</strong></h5><ul><li>Generated reports automatically include a <strong>dual mode label</strong>, indicating the chromatogram's origin (Front/Back).</li><li>All standard report formatting options remain supported.</li></ul><h5><strong>Data Evaluation</strong></h5><ul><li>The mode supports <strong>independent processing</strong> for each chromatogram.</li><li>All standard <strong>reprocessing operations</strong>—such as integration, calibration application, and result analysis - are supported independently for each position.</li></ul><h4><strong>Conclusion</strong></h4><p>Clarity’s Dual Chromatogram Mode offers robust functionality for dual-detector systems, providing synchronized acquisition while maintaining the independence of signal processing. This ensures analytical accuracy, ease of comparison, and flexible evaluation, especially in multi-detector chromatographic setups.</p><blockquote><p>For more information, see the Dual Chromatogram Mode manual:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/paper/30514"><strong>Clarity Extension DUAL CHROMATOGRAM MODE</strong></a></li></ul><p>For further learning, watch more instructional content on the:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@DataapexPrague"><strong>DataApex YouTube channel</strong></a></li></ul></blockquote>

Dual Chromatogram Mode in Clarity: A Technical Overview

<h4><strong>1. Introduction</strong></h4><p>This article provides a comprehensive and structured troubleshooting guide for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity chromatography software </strong></a>by DataApex. Based on the instructional video titled <i>“What to do when you have issues with Clarity”</i>, it outlines common errors, diagnostic methods, and recommended procedures for addressing technical problems in both software and hardware configurations.</p><h4><strong>2. Required Tools and Environment</strong></h4><p>To effectively troubleshoot Clarity, ensure you have access to the following:</p><ul><li><strong>Clarity Software</strong> (version 9.x or 10.x)</li><li><strong>Hardware key (HW key)</strong> – for license authorization</li><li><strong>Windows administrator privileges</strong></li><li><strong>Connected instrumentation</strong> (GC/LC systems, A/D converters)</li><li><strong>Diagnostic tools</strong>:<ul><li>Windows Event Viewer</li><li>Clarity Device Configuration Manager</li></ul></li></ul><h4><strong>3. Common Errors and Solutions</strong></h4><h5><strong>3.1 File Access Errors</strong></h5><p><strong>Symptom</strong>:</p><ul><li>Error messages such as:<br>"Unknown error accessing ... Temp\Clarity\$ch1run.raw"</li></ul><p><strong>Cause</strong>:</p><ul><li>Lack of write permissions to the Temp directory.</li></ul><p><strong>Solution</strong>:</p><ol><li>Ensure the Windows user has full access to the Temp\Clarity folder.</li><li>Run Clarity as <strong>Administrator</strong> to bypass restricted permissions.</li></ol><h5><strong>3.2 Hardware Key Not Recognized</strong></h5><p><strong>Symptoms</strong>:</p><ul><li>"Clarity is unable to find HW key"</li><li>"Missing keylock drivers"</li><li>"Blocked HW key"</li></ul><p><strong>Causes</strong>:</p><ul><li>Key not plugged in, damaged USB port, or missing driver.</li></ul><p><strong>Solution</strong>:</p><ol><li>Reinsert the HW key into a direct USB port (avoid USB hubs).</li><li>If drivers are missing or outdated, reinstall the HW key drivers and restart the PC.</li><li>Contact DataApex support if the key is blocked or invalid.</li></ol><h5><strong>3.3 Visual C++ Redistributable Installation Fails</strong></h5><p><strong>Error</strong>:</p><ul><li>"Exit code: 1618" during Clarity installation.</li></ul><p><strong>Cause</strong>:</p><ul><li>Another installation is already running (e.g., Windows Update).</li></ul><p><strong>Solution</strong>:</p><ul><li>Wait for the current installation to finish, restart the system, and rerun the Clarity installer.</li></ul><h5><strong>3.4 Instrument Communication Failure</strong></h5><p><strong>Error</strong>:</p><ul><li>"Cannot establish communication with XXX SN xxx!"</li></ul><p><strong>Possible Causes</strong>:</p><ul><li>Instrument is turned off or disconnected.</li><li>Wrong or missing driver.</li><li>Incorrect COM port or IP address.</li></ul><p><strong>Solution</strong>:</p><ol><li>Power on the instrument and verify all connections.</li><li>Use Clarity → System Configuration → Devices to verify driver and port settings.</li><li>Restart the driver or reassign the port if needed.</li></ol><h5><strong>3.5 RAW File Creation Errors</strong></h5><p><strong>Error</strong>:</p><ul><li>"Cannot create RAW file"</li><li>"Cannot create $ch1temp.raw"</li></ul><p><strong>Cause</strong>:</p><ul><li>Clarity fails to rename the RAW file due to a file conflict or improper termination.</li></ul><p><strong>Solution</strong>:</p><ul><li>Ensure the previous acquisition was closed properly.</li><li>Clear error dialogs and restart the acquisition.</li></ul><h5><strong>3.6 Project Change Requires Restart</strong></h5><p><strong>Error</strong>:</p><ul><li>"Changing project requires to restart instrument."</li></ul><p><strong>Solution</strong>:</p><ul><li>Save your changes and allow the instrument to restart as prompted.</li></ul><h5><strong>3.7 Configuration File Corruption or Version Conflict</strong></h5><p><strong>Errors</strong>:</p><ul><li>"Configuration file is not valid"</li><li>"Incompatible device driver"</li></ul><p><strong>Cause</strong>:</p><ul><li>Attempting to load a project or method created in a newer software version.</li></ul><p><strong>Solution</strong>:</p><ol><li>Use backup files provided by Clarity.</li><li>Reconfigure devices manually if needed.</li><li>Install matching drivers compatible with your current Clarity version.</li></ol><h5><strong>3.8 Method and Table Errors</strong></h5><p><strong>Common Issues</strong>:</p><ul><li>"Fraction table contains overlapping time intervals"</li><li>"Too many peaks in chromatogram"</li><li>"Method file does not match instrument configuration"</li></ul><p><strong>Solution</strong>:</p><ul><li>Adjust fraction time intervals to prevent overlaps.</li><li>Tweak peak detection settings (width, threshold).</li><li>Modify or recreate the method to reflect hardware changes.</li></ul><h4><strong>4. General Troubleshooting Workflow</strong></h4><ol><li><strong>Reboot your PC</strong> – resolves many temporary issues.</li><li><strong>Check file and folder permissions</strong> – especially the Temp and project directories.</li><li><strong>Verify hardware connections</strong> – including USB and serial ports.</li><li><strong>Update or reinstall drivers</strong> – HW key, devices, or Clarity modules.</li><li><strong>Restore from backups</strong> – when configuration or method files are corrupted.</li></ol><h4><strong>5. Analysis and Best Practices</strong></h4><ul><li><strong>70% of reported issues</strong> are related to permissions, HW key problems, or outdated drivers.</li><li>Always keep <strong>Clarity and related drivers updated</strong> from the official source.</li><li>Ensure <strong>project and method files are compatible</strong> with the Clarity version you are using.</li><li>Use <strong>administrative privileges</strong> when installing or configuring hardware/software.</li></ul><h4><strong>6. Conclusion</strong></h4><p>Clarity is a robust chromatography software platform, but like any system, it may encounter operational or configuration errors. This guide outlines practical and verified troubleshooting techniques derived from real-world scenarios shown in the DataApex tutorial video.</p><p>For persistent or unclear issues, consult the DataApex Support Portal for documentation, driver downloads, and direct contact with technical support.</p><blockquote><h5><strong>Quick Tips Recap</strong></h5><ul><li>Always <strong>start with a reboot and check permissions</strong>.</li><li><strong>Reinstall drivers</strong> if devices or keys are not recognized.</li><li>Use <strong>correct project/method versions</strong> matching your Clarity installation.</li></ul></blockquote>

Troubleshooting Guide: Resolving Issues with Clarity Chromatography Software

<p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity</strong></a><strong> is a professional chromatography data system (CDS) developed by DataApex. This article provides a technical guide to how Clarity organizes and stores its files. You will learn about each key directory, the types of data stored, best practices for backup and transfer, and file dependencies that must be preserved.</strong></p><h4><span style="font-family:&quot;Segoe UI Emoji&quot;,sans-serif;"><strong>🗂️</strong></span><strong> Main Folder Structure</strong></h4><p>Once Clarity is installed, several important folders are created within the installation directory:</p><h5><strong>1. Bin Folder</strong></h5><ul><li>Contains the <strong>Clarity software executable files</strong> and essential utilities:<ul><li><strong>IQ</strong> (Installation Qualification)</li><li><strong>Launch Manager</strong></li></ul></li><li>No user modification or backup is needed here.</li></ul><h5><strong>2. CFG (Configuration) Folder</strong></h5><ul><li>Stores <strong>station configuration and user-specific settings</strong>.</li><li>Includes:<ul><li><strong>Device configuration files</strong></li><li><strong>User layout files (.DSK)</strong></li><li><strong>Audit trails</strong></li><li><strong>Diagnostic data</strong></li></ul></li><li>Essential for <strong>backup</strong> and <strong>station transfer</strong>.</li></ul><h5><strong>3. DataFiles Folder</strong></h5><ul><li>Stores <strong>all chromatographic measurement data</strong>.</li><li>Organized by <strong>projects</strong>—details provided in the next section.</li></ul><h5><strong>4. Documentation Folder</strong></h5><ul><li>Includes <strong>data sheets</strong>, <strong>user manuals</strong>, and relevant documents for the installed software version.</li></ul><h5><strong>5. uninstall.exe</strong></h5><ul><li>Used to <strong>uninstall Clarity</strong> from the system.</li></ul><h4><strong>What Should Be Backed Up?</strong></h4><p>When backing up or transferring a Clarity installation, it is critical to retain the following:</p><ul><li>The entire <strong>CFG folder</strong> – contains configuration, settings, and audit data.</li><li>The <strong>DataFiles folder</strong> – contains all project data and chromatograms.</li><li>Key files:<ul><li>configuration.cfg – station/device setup</li><li>desktop.dsk – user interface layout</li><li>clarity.psw – password storage</li></ul></li></ul><h4><strong>Understanding the DataFiles Folder Structure</strong></h4><p>Clarity uses a <strong>project-based architecture</strong> for storing measurement data. The structure of the DataFiles folder is as follows:</p><p><strong>Top-Level Contents of DataFiles</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Top_Level_Contents_of_Data_Files_ce779a1797.jpg" alt="DataApex: Table 1 Top-Level Contents of DataFiles" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Top_Level_Contents_of_Data_Files_ce779a1797.jpg 245w," sizes="100vw" width="1600" height="377"></p><p><strong>Inside a Project Folder</strong></p><p>Each project folder contains the following:</p><ul><li><strong>Main project directory</strong>:<ul><li>Method files (*.met)</li><li>Sequence files (*.sqt)</li><li>SST files (*.sst)</li></ul></li><li><strong>Subfolders</strong> (created by default):<ul><li>Calib – for <strong>calibration chromatograms</strong> and <strong>standards</strong></li><li>Data – for <strong>unknown samples</strong> and <strong>bypass measurements</strong></li></ul></li></ul><p>Users may <strong>create subfolders inside Calib and Data</strong> for better organization by date, sample type, etc.</p><p><span style="font-family:&quot;Segoe UI Emoji&quot;,sans-serif;">⚠️</span> <strong>Important</strong>: Do not create subfolders outside of Calib and Data. Improper structure may break internal file links and lead to system errors.</p><h4><span style="font-family:&quot;Segoe UI Emoji&quot;,sans-serif;"><strong>📁</strong></span><strong> Example Files and Their Functions</strong></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Example_Files_and_Their_Functions_f1924494e5.jpg" alt="DataApex: Table 2 Example Files and Their Functions" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Example_Files_and_Their_Functions_f1924494e5.jpg 245w," sizes="100vw" width="1600" height="639"></p><h4><strong>Best Practices</strong></h4><ul><li><strong>Regularly back up</strong> both CFG and DataFiles folders.</li><li><strong>When transferring Clarity to a new PC</strong>:<ol><li>Install Clarity as usual.</li><li>Copy over the CFG and DataFiles folders.</li></ol></li><li><strong>Avoid moving or editing files manually</strong> outside of permitted folders (Calib, Data).</li></ul><h4><strong>Conclusion</strong></h4><p>Proper file management in Clarity ensures a stable and reliable workflow. By following the recommended folder structure and backup strategy, users can safeguard their analytical data, simplify system migration, and avoid common issues.</p>

Clarity File Storage and Organization: A Complete Guide

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