<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezGCMS Library in the week of 22nd July 2024? Check out new documents from the field of the gas phase, especially GC and GC/MS techniques!</strong></p>
<blockquote>
<h4>👉 <a href="https://gcms.labrulez.com/library"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT GCMS AND RELATED TECHNIQUES</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p>
</blockquote>
<p>This week we bring to you poster presentations from <a href="https://gcms.labrulez.com/events/568"><strong>ASMS 2024</strong></a> by <strong>LECO</strong>, <strong>Agilent Technologies</strong>, and <strong>Thermo Fisher Scientific</strong>! And as a bonus two application notes from <strong>Shimadzu</strong> and <strong>Thermo Fisher Scientific</strong>.</p>
<blockquote>
<p>💡 <strong>+ 100 ASMS posters and presentations <a href="https://gcms.labrulez.com/library?mainauthorItems=284">available in the LabRulezGCMS library</a></strong> (unique search and filtering)</p>
</blockquote>
<h4><strong>1. LECO: <a href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Long_Lifetime_Ion_Detector_GC_G_Cx_GC_TOFMS_MS_ASMS_24_LPSS_326_4c9e1fb7a1.pdf">A Novel Long-Lifetime Ion Detector for GC-TOFMS and GCxGC-TOFMS with Sub-Femtogram Detection Limits</a></strong></h4>
<ul>
<li>poster - ASMS 2024</li>
</ul>
<p><strong>Introduction:</strong> Ion detection efficiency and fast response times are critical aspects of the ion detectors used in time-of-flight mass spectrometry (TOFMS). With modern advancements in ionization, ion optics, and ion transfer efficiency, TOFMS systems are capable of generating high ion flux onto the ion detectors. Having an ion detector that is capable of maintaining optimal performance over a long period of time while accepting high amounts of ion flux is a significant challenge in development of TOFMS instrumentation. Improvements in detector life will minimize maintenance costs and improve TOFMS user experience. A novel long-lifetime ion detector was developed in partnership with El Mul Technologies for use in LECO’s Pegasus® BTX GC and GCxGCTOFMS. The system was thoroughly evaluated for sensitivity and robustness, including longevity of the detector.</p>
<p><strong>Conclusions:</strong> The Pegasus BTX GC(GCxGC)-TOFMS with a novel Long-Lifetime Ion Detector has demonstrated the following performance characteristics:</p>
<ul>
<li>Sub-2 fg IDL for GC-TOFMS and Sub-fg IDL for GCxGC-TOFMS </li>
<li>Linear dynamic range of 5 orders of magnitude in GC-TOFMS and 4 orders of magnitude in GCxGC-TOFMS</li>
<li>Robust operation: the detector bias remains consistent and BTX peak intensity remains consistent over long periods of time, even with large numbers of samples acquired and significant amounts of charge accumulation on the detector.</li>
</ul>
<h4><strong>2. Thermo Fisher Scientific: <a href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/po_142_utilizing_hydrogen_pesticides_food_gc_ms_po142_asms2024_en_7ed0951b93.pdf">Utilizing hydrogen carrier gas for sensitive analysis of pesticides in food using gas chromatography mass spectrometry</a></strong></h4>
<ul>
<li>poster - ASMS 2024</li>
</ul>
<h5><strong>Abstract</strong></h5>
<p><strong>Purpose:</strong> To demonstrate the performance of the Thermo Scientifc HeSaverH2Safer kit for iConnect split-splitless (SSL) injection module for trace analysis of pesticides using H2 as a carrier gas as a safe and sustainable solution for laboratory operations.</p>
<p><strong>Methods:</strong> In this study, hydrogen was used as a carrier gas for GC-MS/MS analysis of trace levels of pesticides in food. The GC was fitted with a new hydrogen safer split/splitless injector, with chromatographic separation performed on a TG-5 SIL MS(30 m × 0.25 mm, 0.25 μm) capillary GC column. The chromatographic conditions applied ensured critical pair separation was achieved. The GC-MS/MS system was equipped.</p>
<h5><strong>Conclusions</strong></h5>
<ul>
<li>The use of the HeSaver-H2Safer technology allows for a safe and compliant use of hydrogen as an alternative carrier gas in GC/GC-MS applications without the need to install a hydrogen sensor and removes any risk of unwanted reactions with the sample in the hot SSL injector.</li>
<li>When used with hydrogen, the limited carrier gas consumption offered by the HeSaverH2Safer mode permits a very controlled system demand for hydrogen, making this solution ideal for laboratories working with hydrogen generators.</li>
<li>Migrating the GC-MS/MS method from helium to hydrogen requires an adaptation of method parameters to address (i.e., retention time shift and different fragmentation patterns).</li>
<li>97% of pesticides in baby food and 98% in honey showed a linear response in the concentration range 0.005–0.500 mg/kg and the relative standard deviation for N=5 repeats of each sample type at 0.01 mg/kg was equal or lower than 10% for 98% of the evaluated compounds with an advanced electron ionization (AEI) source to increase sensitivity for analyte detection. Quantification and assessment of critical regulatory requirements, such as linearity and sensitivity, were performed in a single software.</li>
</ul>
<h4><strong>3. Agilent Technologies: <a href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/po_142_utilizing_hydrogen_pesticides_food_gc_ms_po142_asms2024_en_7ed0951b93.pdf">Analyzing Nitrosamines with Hydrogen Carrier Gas: GC/MS/MS Analysis of Nitrosamines in Sartan Drugs</a></strong></h4>
<ul>
<li>Poster Reprint - ASMS 2024</li>
</ul>
<p><strong>Introduction:</strong> The limited availability of helium has been a concern, leading to a growing interest in switching to alternative carrier gases like hydrogen. Hydrogen offers several advantages over helium1 , including faster run times and improved chromatographic resolution. Helium is commonly used as a carrier gas for analyzing nitrosamine impurities in pharmaceuticals. However, there are concerns that nitrosamines may react with hydrogen, forming unwanted byproducts. To ensure that using hydrogen as a carrier gas does not impact spectral quality, we evaluated its suitability for analyzing eight impurities:</p>
<ul>
<li>N-nitrosodimethylamine (NDMA),</li>
<li>N-nitrosomethylethylamine (NMEA),</li>
<li>N-nitrosodiethylamine (NDEA),</li>
<li>N-nitroso-ethylisopropylamine (NEIPA),</li>
<li>N-nitrosodiisopropylamine (NDIPA),</li>
<li>N-Nitrosodipropylamine (NDPA),</li>
<li>N-nitrosodi-n-butylamine (NDBA),</li>
<li>N-Nitrosopiperidine (NPIP).</li>
</ul>
<p>Hydrogen carrier gas was used with the 7000E and 7010 Series GC/TQ systems equipped with the HydroInert and HES ion sources respectively, assessing spectral quality, linearity, repeatability, recovery, and compliance with nitrosamine analysis regulations.</p>
<p>MassHunter 13 software suite provided the tools for data acquisition and processing to be performed under a unified compliance environment. OpenLab Electronic Content Management (ECM) XT includes native tools to facilitate compliance, including the ability to create users with affiliated permissions, the generation of audit trails, and remote data storage.</p>
<p><strong>Conclusions:</strong></p>
<ul>
<li>The 7000E and 7010 Series GC/TQ systems equipped with the HydroInert and HES, respectively, exhibited exceptional performance in determining eight nitrosamine drug impurities in sartan drug products and substances using hydrogen carrier gas.</li>
<li>The method was validated at 30 ppb with acceptable recovery and long-term repeatability. </li>
<li>The 7010 Series GC/TQ system enabled a smooth switch between helium and hydrogen carrier gas without compromising the performance of the methods. </li>
<li>Both the HydroInert and the HES sources facilitated achieving the necessary detection limits for the tested impurities.</li>
</ul>
<h4><strong>4. Agilent Technologies: <a href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/po_142_utilizing_hydrogen_pesticides_food_gc_ms_po142_asms2024_en_7ed0951b93.pdf">Analysis of Essential Oils Using a Comprehensive GCxGC with a Reverse Flow Modulator Combined with High Resolution GC/MS</a></strong></h4>
<ul>
<li>Poster Reprint - ASMS 2024</li>
</ul>
<p><strong>Introduction:</strong> Essential oils are typically analyzed to determine their chemical composition contributing to flavor and quality as well as to evaluate their authenticity and identify adulterants. Essential oils are often complex samples with high number of volatile and semi-volatile components that include many isomers with highly similar spectra and close Retention Indices (RIs) when separated on a single column. Hence, a comprehensive GCxGC approach is preferable to achieve an appropriate chromatographic separation for this sample matrix. In this study we used comprehensive GCxGC coupled to a high-resolution accurate mass GC/MS to identify the essential oil  components. Here we also investigate approaches for comparison of the complex GCxGC essential oil data.</p>
<p><strong>Conclusions:</strong></p>
<ul>
<li>Several comprehensive GCxGC configurations and method using the high-resolution GC/Q-TOF and RFM were developed and optimized using a diesel sample. </li>
<li>Essential oils were analyzed using the optimized GCxGC approach, and the workflows for compound identification and statistical analysis were evaluated.</li>
</ul>
<h4><strong>5. Shimadzu: <a href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/an_01_00746_en_e14cf49b7b.pdf">Greenhouse Gas Analyzer</a></strong></h4>
<ul>
<li>Application</li>
</ul>
<p><strong>User Benefits:</strong></p>
<ul>
<li>Simultaneously measures the 3 main greenhouse gases: CH₄, CO₂, and N₂O.</li>
<li>Offers short analysis cycle times and is equipped with an AOC-6000 Plus syringe injection system to analyze large numbers of samples.</li>
<li>Uses nitrogen as a carrier gas for lower running costs.</li>
</ul>
<p><strong>Introduction:</strong> Greenhouse gases are a major cause of climate change, and carbon dioxide, (CO₂), methane (CH₄), and nitrous oxide (N₂O) are considered the 3 primary greenhouse gases. Although CO₂ accounts for the majority of greenhouse gas emissions followed by CH₄, reducing CH₄ emissions is considered important because CH₄ has a warming effect that is more than twenty times greater than CO₂. At the United Nations Climate Change Conference (COP26) in November 2021, over 100 countries joined the Global Methane Pledge, which pledges by 2030 to reduce global CH₄ emissions by 30 % compared to 2020 levels. The Greenhouse Gas Analyzer is a gas chromatography system that has been developed by Shimadzu in collaboration with the National Agriculture and Food Research Organization (NARO) (Fig. 1). Measuring levels of CH₄, CO₂, and N₂O simultaneously in a single sample is difficult, but the Greenhouse Gas Analyzer has been designed to do this simultaneously, which reduces analytical errors and improves the sampling speed, giving researchers a valuable tool in reducing greenhouse gas emissions.</p>
<p>This Application News describes using the Greenhouse Gas Analyzer to simultaneously measure CO₂, CH₄, and N₂O levels.</p>
<p><strong>Conclusion:</strong> The Greenhouse Gas Analyzer can simultaneously measure 3 greenhouse gases in atmospheric air in a single analysis. Each analysis by the Greenhouse Gas Analyzer is performed in 8 minutes with an analysis cycle time of 10 minutes.</p>
<h4><strong>6. Thermo Fisher Scientific: <a href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/an_002695_gc_triplus_rsh_smart_sample_prep_environ_an002695_na_en_77a5aa2300.pdf">An automated approach for the analysis of VOCs in drinking and surface water by using the TriPlus RSH SMART VOC Sample Prep Station</a></strong></h4>
<ul>
<li>Application - Environmental</li>
</ul>
<p><strong>Goal:</strong> The aim of this study is to demonstrate the suitability of the Thermo Scientific™ TriPlus™ RSH SMART VOC Sample Prep Station for the analysis of volatile organic compounds (VOCs) in drinking and surface water by using a fully automated sample preparation workflow.</p>
<p><strong>Keywords:</strong> Automated sample preparation, TriPlus RSH SMART Sample Prep Station, volatile organic compounds, VOCs, drinking water, surface water, gas chromatography, TRACE 1610 GC, single quadrupole mass spectrometry, ISQ 7610 GC-MS.</p>
<p><strong>Conclusions:</strong> The results of these experiments demonstrate that the automated sample preparation capability of the TriPlus RSH SMART VOC Sample Prep Station coupled to the ISQ 7610 GC-MS system provides an ideal solution for water testing laboratories looking to improve productivity and deliver confident results.</p>
<ul>
<li>Static headspace is a convenient solventless extraction technique for volatiles in water with almost no sample preparation required. </li>
<li>Unattended operations of up to 210 samples can be achieved with the automated calibration dilution and ISTD addition workflows.</li>
<li>The automated addition of fresh reagent just before the incubation increases the stability of ISTD/surrogates mix, therefore improving the accuracy of the quantitation of target analytes during data reprocessing.</li>
<li>The TriPlus RSH SMART VOC Sample Prep Station ensures increased sample integrity for highly reliable quantitative analysis, and reduced errors or possible cross-contaminations, maximizing the productivity of the laboratory. Additionally, it allows saving valuable analyst time and improving safety by limiting the user’s exposure to toxic chemicals. </li>
<li>The integrated control for both autosampler and GC-MS in a single CDS ensures a streamlined automated workflow from on-line sample preparation to sequence setup, data acquisition, and reporting. </li>
<li>Suitability of headspace sampling for analysis of VOCs was demonstrated with R2  &gt; 0.990, RRF %RSD &lt; 20%, and calculated amount within 20% of the spiked concentration. </li>
<li>Inter-day reproducibility was demonstrated by running three batches of samples bracketed with QCs. Average absolute peak area %RSD across the entire evaluation period was &lt;20%, the QC calculated amount was within 20% of the expected value, and the calculated recovery was within 70–130%, with the only exception of 1,2-dibromo-3-chloropropane for which the % recovery was 132. </li>
<li>Reliable quantitative analysis was achieved for drinking water samples and surface water samples analyzed in three different batches across six working days. All the sample results were compliant with the thresholds set by the current EU regulation.</li>
</ul>


News from LabRulezGCMS Library - Week 30, 2024

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 22th July 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p>
<blockquote>
<h4>👉 <a href="https://lcms.labrulez.com/library"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p>
</blockquote>
<p>This week we bring to you poster presentations from <a href="https://lcms.labrulez.com/events/568"><strong>ASMS 2024</strong></a> by <strong>Agilent Technologies</strong>, <strong>Shimadzu</strong>, <strong>Thermo Fisher Scientific</strong>, and <strong>Waters Corporation</strong>!</p>
<blockquote>
<p>💡 <strong>Almost 1000 ASMS posters and presentations <a href="https://lcms.labrulez.com/library?mainauthorItems=284">available in the LabRulezLCMS library</a></strong> (unique search and filtering)</p>
</blockquote>
<h4><strong>1. Waters Corporation: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/2023_asms_king_liver_178c54e990.pdf">HIGH THROUGHPUT PLASMA PROFILING OF HUMAN LIVER DISEASE SAMPLES USING RAPID CHROMATOGRAPHY AND A MULTI- REFLECTING TIME-OF-FLIGHT MASS SPECTROMETER</a></strong></h4>
<ul>
<li>poster - ASMS 2024</li>
</ul>
<p><strong>Introduction:</strong> Metabolomics is a valuable research strategy in the determination  FIG. 5  FIG. 1  FIG. 7  FIG. 3  of disease progression, disease stratification, the identification of  biological markers for early diagnosis and for the development of  new therapeutics. Acute-on-chronic liver failure (ACLF) is a  condition characterized by systemic inflammation, multiple organ  failure, and high short-term mortality1. ACLF develops from the  acute decompensation of cirrhosis and currently there is no  biomarker identified for the early diagnosis of this disease.  Therefore, fully understanding the role metabolites and lipids play  in the progression of ACLF and its underlining mechanisms could  greatly help determine new diagnostic markers driving  development of new therapeutics. Investigating biomarkers for  large scale studies requires robust, high-throughput analytical  methods.</p>
<p>Rapid microbore metabolic profiling (RAMMP) methods and  conventional high-resolution mass spectrometry have previously  been shown to considerably reduce analysis time2-3. Here we  compare these methods with a vacuum jacketed column  technology (VJC)4-5 and ultra high-resolution mass spectrometry6  for the analysis of plasma from liver disease patients by reversed-  phase (RP) for lipid and hydrophilic interaction chromatography  (HILIC) for small molecules.</p>
<p><strong>Conclusion:</strong></p>
<ul>
<li>Conventional LC-MS methods used for large cohort studies suffer from batch response variation.  The rapid chromatographic methods reduce this impact and demonstrated reproducible response  across the entire batch of 168 injections per polarity (Fig. 2).</li>
<li>The SELECT SERIES MRT mass spectrometer generated highly accurate mass measurements  (&lt;1 ppm), improving annotation confidence for both lipids and small molecules, including essential  amino acids and carnitines from the HILIC analysis (Fig. 3).</li>
<li>PCA of the RAMMP data showed good analytical reproducibility from the clustered QC samples and  group separation with some overlap between the different disease groups (Fig. 4).</li>
<li>Group comparisons were performed by OPLS-DA where statistically significant features were  highlighted for database annotation (Fig. 5).</li>
<li>A number of lipids classes, particularly lysophosphatidylcholines (LPC), phosphatidylcholines (PC)  and sphingomyelins (SM), showed dysregulation in the diseased samples when compared to healthy  controls and were annotated through Lipostar2 with mass accuracies between 0.1-0.3 ppm (Fig. 6).</li>
<li>For the HILIC small molecule analysis, differences in amino acid response were detected in  comparisons between diseased and healthy controls.</li>
<li>The SELECT SERIES MRT MS achieved mass resolution of 170-200K FWHM at 30 Hz compatible with  RAMMP (a) and VJC (b) methodologies for example lipids, PC36:1 (0.18 ppm) and SM42:3, O2 (0.16  ppm), with the VJC providing better peak shape and resolution of adjacent peaks (Fig. 7)</li>
<li>Comparison of the RAMMP and VJC negative lipid analysis showed very similar distribution of  disease and healthy control groups demonstrating minimal impact on the overall statistics (Fig. 8).</li>
<li>Both RAMMP and VJC methodologies combined with the SELECT SERIES MRT provided highly  accurate reproducible metabolic profiling for a biomarker study, with the VJC providing improved  peak fidelity complemented by the fast scanning high resolution capabilities of the SELECT SERIES  MRT.</li>
<li>This work demonstrated an accurate, reproducible and high throughput metabolomic method for the  rapid screening of a large number of biological samples for biomarker discovery.</li>
</ul>
<h4><strong>2. Thermo Fisher Scientific: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/po_143_characterization_structure_adenopeptin_lc_hram_ms_po143_asms2024_en_7ae893f36c.pdf">In-depth characterization and structure elucidation of non-ribosomal peptides related to adenopeptin using LC-HRAM-MSⁿ</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<h5><strong>Abstract</strong></h5>
<p><strong>Purpose:</strong> Characterization of adenopeptin and related peptaibol analogs in a crude fermentation broth extract using LC-HRAM-MSn to identify adenopeptin congeners and elucidate their structures. Methods: Adenopeptin and its analogs were separated using reversed phase chromatography and analyzed using multi-stage fragmentation on a Thermo Scientific Orbitrap IQ-X Tribrid mass spectrometer. </p>
<p><strong>Results:</strong> Several analogs of adenopeptin differing by one or more methyl groups could be detected and the site of their modification could be narrowed to specific amino acid residues based on their fragmentation patterns.</p>
<h5><strong>Conclusions</strong></h5>
<p>The LC/MS analysis of the crude adenopeptin extract revealed the presence of eight analogs above 0.5% relative MS-abundance, mainly resulting from ”demethylations”, i.e., substitution of Aib for Iva residues. Their modification sites could be determined based on shifts in the fragmentation pattern relative to adenopeptin. Additionally, a potentially crucial substitution of pipecolic acid with proline could be detected and verified based on MS3 spectral evidence for compound 4.</p>
<h4><strong>3. Thermo Fisher Scientific: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/po_146_evaluation_automated_low_flow_lc_ms_setup_po146_asms2024_en_1d935eb142.pdf">Evaluation of an Automated “Quick” Optimization Routine to Streamline Low-Flow LC-MS Setup</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<h5><strong>Abstract</strong></h5>
<p><strong>Purpose:</strong> When setting up low flow LC-MS experiments, a user regularly positions an  emitter at an arbitrary fixed distance from the inlet based on visual alignment. A  prototype ion source featuring an emitter mounted on a 3D motorized stage was used  to evaluate a “quick” optimization strategy used to define the active position for  subsequent LC-MS measurements.</p>
<p><strong>Methods:</strong> The basis of the quick optimization routine was established using m/z- and  position-dependent intensity distributions. The routine was evaluated against two  methods to manually set the emitter position.</p>
<p><strong>Results:</strong> All optimization strategies yielded a statistically equivalent number of protein  and peptide identifications. However, the quick optimization routine yielded superior  peak area reproducibility that translated into significantly better emitter-to-emitter  consistency.</p>
<h4><strong>4. Shimadzu: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/ASMS_poster_PESI_v_DMS_170524_fed75d0c1e.pdf">DPiMS-MS (PESI) combined with vacuum differential mobility spectrometry for rapid forensic analysis.</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<p><strong>Introduction</strong></p>
<ul>
<li>Ambient ionisation (AI) mass spectrometry techniques facilitate rapid turn-around of samples, often in a walk-up format with limited sample preparation, with obvious benefits for forensic applications.</li>
<li>The absence of a chromatographic separation enables sub-minute data acquisition. However, this comes with the cost of greater chance of problems with chemical noise and/or interference between isomeric/isobaric compounds.</li>
<li>One way to remove chemical noise and to separate interfering compounds is to add an ion mobility separation to the method.</li>
<li>Here we implement a novel low pressure differential mobility on a DPiMS-8060 (PESI) system and demonstrate performance for steroids and small molecule drugs in saliva.</li>
</ul>
<p><strong>Conclusion</strong></p>
<ul>
<li>These results demonstrate the potential of vDMS to enhance AIMS by removing chemical noise and separating isobaric species.</li>
<li>DPiMS-vDMS-8060 (PESI) retains the high acquisition speed of PESI while adding an additional dimension of separation.</li>
<li>The additional selectivity may lower the LOQ and reduce false positives.</li>
<li>vDMS-MS methods can be applied in a wide range of complex matrices (e.g. urine, plasma).</li>
</ul>
<h4><strong>5. Shimadzu: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/MP_045_Targeted_quantitative_screening_pesticides_using_high_resolution_DIA_spectral_library_matching_238a221a67.pdf">Targeted quantitative screening pesticides in food matrices using high resolution DIA spectral library matching.</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<p><strong>Overview</strong></p>
<ul>
<li>A single, generic untargeted data independent acquisition (DIA) method was developed for the analysis of extended panels of pesticides in food matrices.</li>
<li>The high-resolution LC-MS/MS method was designed for multi-residue screening and quantitation of pesticides to meet the European Union SANTE 11312/2021 v2 guidelines capable of working with extended target pesticide panels.</li>
<li>The MS and DIA-MS/MS method was applied to quantify a routine panel of pesticides but also included an additional target panel of fungicides for qualitative screening.</li>
</ul>
<p><strong>Conclusions</strong></p>
<ul>
<li>Using a single generic LC-MS and DIA-MS/MS method targeted quantitation using ion ratio confirmation (to meet the EU SANTE guidelines) and extended search capabilities with library identification were supported.</li>
<li>The method results in the quantitation of &gt;250 pesticides at or below the default MRL concentration (10 µg/kg).</li>
</ul>
<h4><strong>6. Shimadzu: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/MP_045_Targeted_quantitative_screening_pesticides_using_high_resolution_DIA_spectral_library_matching_238a221a67.pdf">Identification of positional isomers of linoleic acid containing phospholipids involved in pancreatic ductal adenocarcinoma.</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<p><strong>Overview</strong></p>
<ul>
<li>To enhance lipid identification, Oxygen Attachment Dissociation (OAD) MS/MS has been applied to provide double bond specific dissociation, enabling C=C position assignments.</li>
<li>High resolution OAD MS/MS was applied in positive and negative ion mode to provide further confidence in lipid identification in a pancreatic adenocarcinoma biomarker study</li>
</ul>
<p><strong>Conclusions</strong></p>
<ul>
<li>OAD-MS/MS is a high specificity fragmentation technique to enable C=C positional assignments in lipids. Analysis is easy to perform alongside CID-MS/MS and spectra are easy to interpret.</li>
<li>OAD-MS/MS has been applied to structurally characterize lipids found to be potential markers of PDAC in a previous study using high resolution metabolomics analysis by LC-CID-MS/MS and DPiMSᵗᵐ.</li>
<li>The use of this new technology aims to enhance pancreatic cancer research by providing more comprehensive identification needed to understand the biological roles of the lipids identified as potential markers of PDAC.</li>
</ul>
<h4><strong>7. Agilent Technologies: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/po_2dlc_6545xt_monitoring_oxidation_mabs_asms_2024_wp030_en_agilent_77f2bb18d5.pdf">Monitoring oxidation in recombinant monoclonal antibodies at subunit level through two-dimensional liquid chromatography coupled with mass spectrometry</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<p><strong>Introduction:</strong> Oxidation, induced by reactive oxygen species, is a prevalent chemical modification in monoclonal antibodies (mAbs), affecting amino acid residues such as cysteine (C), methionine (M), tryptophan (W), tyrosine (Y), lysine (K), and others (Gupta et al., 2022). Each residue&#39;s oxidation uniquely influences the protein&#39;s structure and function. Methionine is highly prone to oxidation in mAbs, typically resulting in the formation of a methionine sulfoxide product with increased polarity. These methionine residues are found in both the antigen-binding (Fab) and effector activity (Fc) domains of a mAb, with those situated on the surface being particularly susceptible to oxidation (Hageman et al., 2019). The increasing popularity of two-dimensional liquid chromatography (2D-LC) has led to a significant rise in the integration of two complementary chromatographic techniques, coupled with mass spectrometry (MS), aiming to provide comprehensive insights into mAb post-translational modifications (PTMs) (Sarin et al., 2022). The proposed 2D WCX-RP-MS workflow enables the analysis of mAbs at the subunit level, providing an automated analytical platform for characterizing and identifying oxidized regions within both the Fc and Fab domains.</p>
<p><strong>Conclusions:</strong> The oxidized peaks not resolved by 1D methods are successfully resolved by the D2 RPC column with additional HC-LC information. It should be highlighted that the proposed method completely uses MS compatible buffers and is a faster method to identify mAb modification and their correlation in different mAb fragments compared to the tedious bottomup approach. A general mAb bottom-up analysis takes up to 24-48 hours with the risk of sample loss, degradation, and induced modifications. In the present study, sample preparation took only 4-10 hours (depending on oxidation incubation), and sample analysis took only 3 hours. Hence, 7-13 hours is enough for the subunit analysis with a low risk of sample loss and degradation. The present study focuses on oxidation as the chemical modification of interest, but the workflow can be extended to other critical charge modifications easily resolved by the WCX column.</p>
<h4><strong>8. Agilent Technologies: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/po_2dlc_6545xt_monitoring_oxidation_mabs_asms_2024_wp030_en_agilent_77f2bb18d5.pdf">Flash Characterization of mAbs Using a Combination of Reagents via Automated On-Line Microdroplet Reaction</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<p><strong>Introduction:</strong> Recently, an unmodified Agilent Jet Stream ESI source (AJS) was used to demonstrate “Flash Characterization” of mAbs by microdroplet facilitated reductions or enzyme digestions with good reproducibility and high yield 1. These results motivated us to evaluate treatments that combined enzymatic digestion, reduction, and/or deglycosylation of mAb in a single microdroplet experiment. New optimization parameters for the Agilent Injection Program on Agilent 1290 Infinity II LC were presented. Microdroplet experiments, including GlycINATOR ® deglycosylation, the combination of IdeS cleavage and DTT reduction and IdeS cleavage and GlycINATOR ® deglycosylation were examined for reproducibility, intact mAb remaining after treatments and simplified subunit characterization. GlycINATOR is an endoglycosidase that cleaves N-linked glycans and leaves the core GlcNAc residue with or without a fucose on the asparagine. Additional antibodies of Herceptin and sigmamab are also studied with the combination treatment in the microdroplet reaction.</p>
<p><strong>Conclusions:</strong> </p>
<ul>
<li>Flash Characterization of mAbs with an automatic injector, combination treatments, and microdroplet reaction in the AJS source demonstrates simplification, reproducibility, and cost reduction of protein characterization. </li>
<li>Combination treatments are viable with microdroplet reactions to accelerate the reduction, cleavage, and deglycosylation of native IgG1 significantly.</li>
</ul>


News from LabRulezLCMS Library - Week 30, 2024

<p><strong>In the week from July 29, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p>
<blockquote>
<h4><a href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Bruker: Advances in Microscopy: A Gateway to Enhanced Research, Drug Development, and Diagnostics</strong></h5>
<ul>
<li>Mo, 29.7.2024 17:00 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/4172">Registration</a></li>
</ul>
<p>Latest advancements in microscopy and applications in kidney disease research and diagnostics.</p>
<h5><strong>2. Bruker: Notch-Mutated Cancer Genomes in 3D</strong></h5>
<ul>
<li>Tu, 30.7.2024 17:00 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/4173">Registration</a></li>
</ul>
<p>Find out what super-resolution microscopy can reveal about epigenetic changes in cancer pathogenesis.</p>
<h5><strong>3. SelectScience/Beckman Coulter: Advancing EV research – First Experiences with the CytoFLEX Nano Flow Cytometer</strong></h5>
<ul>
<li>Tu, 30.7.2024 17:00 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/4109">Registration</a></li>
</ul>
<p>We will discuss the advantages of using fluorescence combined with multiple scatters for further characterization of nanoparticles and extracellular vesicles.</p>
<h5><strong>4. Agilent Technologies: Simplified Measurement of Content Distribution of Pharma Tablets</strong></h5>
<ul>
<li>Tu, 30.7.2024 21:30 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/3905">Registration</a></li>
</ul>
<p>Content distribution of entire pharma tablets can be quickly measured using the component-specific workflow of the LDIR.</p>
<h5><strong>5. Technology Networks/Refeyn: Enhancing the Development of AAV-Based Therapeutics With Mass Photometry</strong></h5>
<ul>
<li>We, 31.7.2024 17:00 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/4180">Registration</a></li>
</ul>
<p>Enter mass photometry, a new bioanalytical technique that can efficiently characterize AAVs and distinguish between full and empty AAVs.</p>


Webinars LabRulezICPMS Week 31/2024

<p><strong>In the 2 weeks from August 5, the following webinars await you in the field of gas / solid phase and especially Raman, FTIR, GC and GC/MS.</strong></p>
<blockquote>
<h4><a href="https://gcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Phenomenex: Solid Phase Extraction Introduction and Theory</strong></h5>
<ul>
<li>Tu, 6.8.2024 19:00 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/4159">Registration</a></li>
</ul>
<p>Understand the benefit of using SPE than other sample preparation techniques.</p>
<h5><strong>2. C&amp;EN/Metrohm: Smart and Actionable: Spectroscopy PAT-Supported Process Operations</strong></h5>
<ul>
<li>We, 7.8.2024 19:00 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/4175">Registration</a></li>
</ul>
<p>How Albemarle Corporation approached the implementation of Spectroscopy PAT to obtain smarter process operations and what you can do to help drive a mindset change in your business.</p>
<h5><strong>3. Agilent Technologies: Advanced GC/MS | GC/HR-MS Based Workflow for Extractable and Leachable Analysis</strong></h5>
<ul>
<li>Th, 8.8.2024 11:30 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/4136">Registration</a></li>
</ul>
<p>Whether you are scouting or conducting a targeted screening, suspect screening, or nontargeted screening, Agilent can help with a wide range of analytical technologies.</p>
<h5><strong>4. Agilent Technologies: Get the Most Out of Your MS: Novel Column Chemistry Raises the Bar on Sensitivity and Data Accuracy</strong></h5>
<ul>
<li>Tu, 13.8.2024 17:00 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/3908">Registration</a></li>
</ul>
<p>We’ll examine how column attributes like bleed, thermal stability, and inertness can translate into practical data quality improvements.</p>
<h5><strong>5. Phenomenex: Advancing Food Testing Using New and Unique GC Column Selectivities</strong></h5>
<ul>
<li>Tu, 13.8.2024 19:00 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/4161">Registration</a></li>
</ul>
<p>Learn about important applications in food testing and current trends. Recieve an overview of available new column selectivity, and relevant applications.</p>
<h5><strong>6. Shimadzu Corporation: Elevating Pharma 4.0: Harnessing CaliberLIMS For Digital Excellence</strong></h5>
<ul>
<li>We, 14.8.2024 08:30 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/4163">Registration</a></li>
</ul>
<p>CaliberLIMS is a robust, scalable, and reliable platform designed to meet the demands of modern laboratories aiming for Pharma 4.0.</p>
<h5><strong>7. Agilent Technologies: UV-VIS-NIR in Battery Development</strong></h5>
<ul>
<li>Th, 15.8.2024 20:00 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/3909">Registration</a></li>
</ul>
<p>Agilent&#39;s portfolio of instruments offers the high performance and flexible accessories needed for pioneering new applications.</p>


Webinars LabRulezGCMS Week 32-33/2024

<p><strong>In the week of August 5, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p>
<blockquote>
<h4><a href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Thermo Fisher Scientific: Navigating changes in regulated software environments</strong></h5>
<ul>
<li>Tu, 6.8.2024 08:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4174">Registration</a></li>
</ul>
<p>Changing regulatory landscapes, observations, and considerations when aligning software records with ALCOA+ principles.</p>
<h5><strong>2. SCIEX: SCIEX OS software summer school - Compound optimization</strong></h5>
<ul>
<li>Tu, 6.8.2024 11:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4156">Registration</a></li>
</ul>
<p>Join us for our summer school series dedicated to enhancing your proficiency with SCIEX OS software.</p>
<h5><strong>3. Phenomenex: Solid Phase Extraction Introduction and Theory</strong></h5>
<ul>
<li>Tu, 6.8.2024 19:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4159">Registration</a></li>
</ul>
<p>Understand the benefit of using SPE than other sample preparation techniques.</p>
<h5><strong>4. Agilent Technologies: Analytical Strategies for Uncovering the Biological Burden of &quot;Forever Chemicals&quot;</strong></h5>
<ul>
<li>We, 7.8.2024 17:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4135">Registration</a></li>
</ul>
<p>Progress and remaining challenges in using high-resolution mass spectrometry (HRMS) to comprehensively capture the total burden of PFASs accumulating in living organisms. </p>
<h5><strong>5. Thermo Fisher Scientific: New ways of targeted translational small molecules quantification</strong></h5>
<ul>
<li>Th, 8.8.2024 11:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4152">Registration</a></li>
</ul>
<p>In this free webinar, Hector shares preliminary data and insights into the critical role of lipids in biochemical processes.</p>
<h5><strong>6. Technology Networks/Bruker: Applying Spatial Multiomics to Degenerative Diseases and Cancer</strong></h5>
<ul>
<li>Th, 8.8.2024 17:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4183">Registration</a></li>
</ul>
<p>Our speaker will discuss how new MALDI workflows can be used to analyze lipids in degenerative disease and Alzheimer’s models, providing more depth to lipid analysis and contextualizing lipid distribution.</p>
<h5><strong>7. Phenomenex: HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns</strong></h5>
<ul>
<li>Fr, 9.8.2024 19:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4087">Registration</a></li>
</ul>
<p>Learn about physical and chemical properties of common reverse phase stationary phases, Find an understanding and recieve steps for stationary phase selection.</p>


 Webinars LabRulezLCMS Week 32/2024

<p><strong>In the 2 weeks from August 5, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p>
<blockquote>
<h4><a href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Agilent Technologies: Elemental Analysis of Lithium-ion Battery Samples using ICP-OES and ICP-MS</strong></h5>
<ul>
<li>Tu, 6.8.2024 21:30 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/3906">Registration</a></li>
</ul>
<p>Elemental Analysis of Lithium-ion Battery Samples using ICP-OES and ICP-MS</p>
<h5><strong>2. C&amp;EN/Metrohm: Smart and Actionable: Spectroscopy PAT-Supported Process Operations</strong></h5>
<ul>
<li>We, 7.8.2024 19:00 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/4175">Registration</a></li>
</ul>
<p>How Albemarle Corporation approached the implementation of Spectroscopy PAT to obtain smarter process operations and what you can do to help drive a mindset change in your business.</p>
<h5><strong>3. Agilent Technologies: Agilent M Lens for ICP-MS and the Semiconductor Industry</strong></h5>
<ul>
<li>Th, 8.8.2024 20:00 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/3907">Registration</a></li>
</ul>
<p>Agilent M Lens for ICP-MS and the Semiconductor Industry</p>
<h5><strong>4. Shimadzu Corporation: Elevating Pharma 4.0: Harnessing CaliberLIMS For Digital Excellence</strong></h5>
<ul>
<li>We 14.8.2024 08:30 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/4163">Registration</a></li>
</ul>
<p>CaliberLIMS is a robust, scalable, and reliable platform designed to meet the demands of modern laboratories aiming for Pharma 4.0.</p>
<h5><strong>5. Agilent Technologies: UV-VIS-NIR in Battery Development</strong></h5>
<ul>
<li>Th 15.8.2024 20:00 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/3909">Registration</a></li>
</ul>
<p>Agilent&#39;s portfolio of instruments offers the high performance and flexible accessories needed for pioneering new applications.</p>


Webinars LabRulezICPMS Week 32-33/2024

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezGCMS Library in the week of 26th July 2024? Check out new documents from the field of the gas phase, especially GC and GC/MS techniques!</strong></p>
<blockquote>
<h4>👉 <a href="https://gcms.labrulez.com/library"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT GCMS AND RELATED TECHNIQUES</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p>
</blockquote>
<p>This week we bring to you applications and other documents by <strong>LECO</strong>, <strong>Agilent Technologies</strong>, <strong>Metrohm</strong> <strong>Shimadzu</strong>!</p>
<h4><strong>1. LECO: <a href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/PEGBTX_GINGER_OIL_HYDROGEN_CARRIER_GAS_203_821_694_1685d36546.pdf">Ginger Oil Analysis and Grade Differentiation with Pegasus BTX Using Hydrogen Carrier Gas</a></strong></h4>
<ul>
<li>application note</li>
</ul>
<p><strong>Key Words:</strong> Fast GC, Hydrogen, TOFMS, Flavor and Fragrance, Essential oil, Ginger oil, Quality control</p>
<p><strong>Introduction:</strong> Ginger essential oil (ginger EO) derives from the rhizome of the plant and has been valued for centuries Zingiber officinale for its aromatic, medicinal, and culinary properties. With a rich history dating back to ancient China and India, ginger oil has been treasured for its versatile applications and therapeutic benefits. Ginger EO is renowned for its warming, spicy aroma and is commonly used in aromatherapy to alleviate nausea, improve digestion, and relieve muscle pain. In culinary applications, it adds depth and complexity to dishes, especially in Asian and  Indian cuisines. Additionally, ginger oil is valued in skincare for its anti-inflammatory and antioxidant properties, promoting healthy skin and hair.</p>
<p>The chemical composition of ginger oil is complex, comprising various bioactive compounds such as gingerol, shogaol, and zingiberene. <strong>Gas Chromatography-Mass Spectrometry</strong> (<strong>GC-MS</strong>) analysis is commonly employed to assess the quality and purity of ginger oil, ensuring it meets industry standards and regulatory requirements. Differentiating essential oil grades is crucial for the perfumery, flavor, and food industries due to several reasons related to quality, consistency, safety, and overall product effectiveness. As an example, high quality EOs are often pure, unadulterated, and extracted using meticulous methods to preserve their natural properties, whil the low-quality ones may be diluted, adulterated, or extracted using less refined methods, potentially compromising their scent and taste profiles and therapeutic qualities.</p>
<p>In this context, perfumery industries often need to be able to assess EO quality parameters in a fast and reliable manner. This application note describes the conversion of a Helium-based method to a fast and reliable Hydrogen-based one using LECO&#39;s novel <strong>BTX GC-TOFMS</strong> instrument. This mass spectrometer offers a wide dynamic range and sensitivity at Pegasus low femtogram levels, allowing for the simultaneous detection of both abundant and trace components without losing any detail. Additionally, ChromaTOF Sync software was used to distinguish between different grades of ginger EO and identify ® markers for each of them.</p>
<p><strong>Conclusions:</strong> This application note demonstrates how the BTX GC-TOFMS is an excellent solution for the rapid analysis of EOs Pegasus using H carrier gas. The results indicate that neither the chromatographic performance nor the mass spectral quality are 2 compromised by using H . A % reduction in runtime was achieved, leading to improved daily throughput. Additionally, 2 60 the Sync software tool effectively clustered the samples based on their origin and quality, enabling the ChromaTOF discovery of characteristic features that differentiate them. The output information received from Sync ChromaTOF allows for more informed usage of raw materials in creating new fragrances and enhances confidence in analyzing routine samples.</p>
<h4><strong>2. Metrohm: <a href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_ra_009_download_d7cc0b953a.pdf">Comparison of SPELEC RAMAN and standard Raman microscopes</a></strong></h4>
<ul>
<li>application note</li>
</ul>
<p><strong>Larger laser spot size can provide representative results with a single measurement</strong></p>
<p>Standard Raman microscopes are traditionally used to perform Raman measurements or Raman spectroelectrochemical experiments when they are coupled with electrochemical equipment. Raman spectra collected with confocal microscopes allows the characterization of very small areas. However, these instruments can exhibit several limitations. SPELEC RAMAN, a new generation of spectroscopic and spectroelectrochemical instruments, offers powerful and interesting features to overcome these issues. In this Application Note, a detailed comparison is made between the main features of a standard Raman instrument and the SPELEC RAMAN by analyzing the results obtained with both instruments.</p>
<p><strong>CONCLUSION</strong></p>
<p>SPELEC RAMAN exhibits a number of competitive advantages over standard Raman microscopes. For instance, the spot size of SPELEC RAMAN allows the characterization of a large area in a single experiment, obtaining representative results with only one measurement. The versatility of the Raman probe is demonstrated since it can be used with different cells. The portability as well as the simplicity of SPELEC RAMAN facilitate the performance of optical experiments. It also ensures the synchronization of electrochemical and optical signals in case Raman spectroelectrochemical measurements are carried out. In addition, DropView SPELEC software allows realtime acquisition of the data and the performance of operando measurements. The easy data treatment and analysis of the results is done using one-click tools.</p>
<h4><strong>3. Shimadzu: <a href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/c103_e421_5c783b2e76.pdf">FTIR Talk Letter (vol. 42)</a></strong></h4>
<ul>
<li>other</li>
</ul>
<p><strong>Table of content</strong></p>
<ul>
<li>Development of Dual Functional Catalytic Materials for CO2 Capture and Selective Hydrogenation and Mechanistic Elucidation of High CO Selectivity Using FTIR Spectroscopy | 02 </li>
<li>Configuration of the AIRsight Infrared Raman Microscope | 06</li>
<li>Notes on Infrared Spectral Analysis – Aliphatic Saturated Hydrocarbons (Paraffins) | 10</li>
<li>Fourier Transform Infrared Spectrophotometers &quot;IRSpirit-X Series&quot; | 16</li>
</ul>
<h4><strong>4. Agilent Technologies: <a href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/an_impurity_analysis_hydrogen_8890_gc_tcd_fid_5994_7590en_agilent_85e4bf7514.pdf">Helium, Argon, Nitrogen, and Hydrocarbon Impurity Analysis in Hydrogen Using an Agilent 8890 GC and TCD/FID System</a></strong></h4>
<ul>
<li>Application Note - Energy &amp; Chemicals</li>
</ul>
<p><strong>Abstract</strong></p>
<p>In this application note, the analysis of helium (He), nitrogen (N₂ ), argon (Ar), and hydrocarbon (HC) impurities in hydrogen (H₂ ) was demonstrated on an <strong>Agilent 8890 gas chromatography</strong> (<strong>GC</strong>) <strong>system</strong> using gas sampling valve injection, capillary column separation, and flame ionization/thermal conductivity detectors (FID/TCD). The system repeatability, sensitivity, and linearity were evaluated. The excellent test results demonstrated that the 8890 GC can provide accurate and precise analysis of the target analytes. In addition, this system can be applied to the quality control of fuel cell vehicles that used hydrogen, according to different regulations such as ISO 14687-2019 and GB/T 37244-2018.</p>
<p><strong>Conclusion</strong></p>
<p>In this application note, an Agilent 8890 GC configured with two gas valves, two types of detectors (FID/TCD), and three capillary PLOT columns, was applied to He, Ar, N₂, and HC impurity analysis in hydrogen. The system performance was evaluated using certified gas standards. The comprehensive assessment covered RT and response repeatability, linearity, quantitation accuracy, and method LODs. The area precision of all test compounds was better than 3.0% at the low concentration level. The LODs for He, Ar, N₂, and methane were 2.6, 0.6, 0.8, and 0.019 ppm, which were far below their quality limits in the ISO 14687-2019 and GB/T 37244-2018 standards. The quantitation accuracy was 92 to 116% across the calibration range. These excellent results demonstrate that the 8890 GC, together with the selected Agilent GC columns, can provide accurate, precise, and sensitive analysis of target components. The test system can reliably be used for the quality control of He, Ar, N₂, CH₄, and other HCs in FCV-grade hydrogen, according to ISO 14687-2019 and GB/T 37244-2018 requirements.</p>


News from LabRulezGCMS Library - Week 31, 2024

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 29th July 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p>
<blockquote>
<h4>👉 <a href="https://lcms.labrulez.com/library"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p>
</blockquote>
<p>This week we bring to you applications, brochures and specifications from <strong>Agilent Technologies</strong>, <strong>Shimadzu</strong>, <strong>Metrohm</strong>, <strong>Thermo Fisher Scientific,</strong> and <strong>Waters Corporation</strong>!</p>
<blockquote>
<p>💡 <strong>Almost 1000 ASMS posters and presentations <a href="https://lcms.labrulez.com/library?mainauthorItems=284">available in the LabRulezLCMS library</a></strong> (unique search and filtering)</p>
</blockquote>
<h4><strong>1. Metrohm: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_v_240_download_23ff0d3438.pdf">Determination of total iodine in thyroid tablets with polarography</a></strong></h4>
<ul>
<li>Application Note</li>
</ul>
<p><strong>Polarography in pharmaceutical analysis: indirect determination of iodine after dry ashing in a muffle furnace</strong></p>
<p>Thyroid hormones are made by the thyroid gland and play an important role in regulating metabolism and growth. Iodine acts as a building block and the specific number of iodine atoms determines the type of the hormone: four for thyroxine (T4) and three for triiodothyronine (T3). The number of iodine atoms is critical for the functionality of thyroid hormones. Levothyroxine and liothyronine (synthetic forms of thyroid hormones T4 and T3) are essential components of thyroid tablets. T4 is less active and needs to convert to the more active T3 to be fully effective. Accurate iodine determination in thyroid tablets is a crucial quality control measure, ensuring the effectiveness and safety of thyroid treatments. A robust method is introduced for indirect determination of total iodine content in thyroid tablets as iodate, according to United States Pharmacopeia (USP) guidelines using the 884 Professional VA and the Multi-Mode Electrode pro.</p>
<h4><strong>2. Shimadzu: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00749_en_efbfc8887d.pdf">Efficient Preparative Purification Workflow of Synthetic Peptide Using Analytical/Preparative Switching LC-MS System</a></strong></h4>
<ul>
<li>Application Note</li>
</ul>
<p><strong>User Benefits</strong></p>
<ul>
<li>A multi-step seamless preparative purification workflow of synthetic peptides can be performed within a single LC-MS system setup.</li>
<li>LabSolutions MD can provide easy investigation for optimal separation conditions for target compounds.</li>
<li>Target compounds can be purified from crude samples with high purity and high recovery based on the excellent identification capability of LCMS-2050.</li>
</ul>
<p><strong>Introduction:</strong> In the 2000s, biopharmaceuticals such as antibody drugs have emerged but there are many challenges to overcome because of their manufacturing process using genetic technology. Consequently, middle molecule drugs are paid attention, peptide therapeutics, one of middle molecule drugs which have advantages of being manufactured at low cost, easily taken into cells because of theirsmall molecular weight, and prevented from degradation by adopting a specific three-dimensional structure when they are taken into human body. Since such peptides are produced by chemical synthesis like small molecule drugs, it is essential to purify, fractionate, and confirm the purity of the final synthesized product. In this article, we present a case study of multi-step seamless preparative purification workflow (optimizing separation conditions, scaling-up, fractionation, and confirming  (purity/recovery) of a peptide using preparative purification liquid chromatograph Nexera Prep (Fig. 1) based on Application News <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00650_en_f326c9720e/an_01-00650-en.pdf"><strong>01-00650-EN</strong></a> and <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00651_en_d88902f27d/an_01-00651-en.pdf"><strong>01-00651-EN.</strong></a></p>
<p><strong>Conclusion:</strong> The seamless preparative purification workflow can be executed using analytical/preparative switching LC-MS system. In addition, LabSolutions MD can automatically create an analytical batch schedule in which various HPLC parametersettings are examined, resulting in efficient optimization ofseparation conditions. Furthermore, LCMS-2050 enables highly selective fractionation of target compounds based on MS trigger. The analytical/preparative switching LC-MS used in this article has both analytical and preparative flow paths, enabling an efficient preparative purification workflow in the preparative purification of peptide including itssynthesis confirmation process.</p>
<Related Applications>

<ol>
<li>Seamless Purification Workflow from Analytical to Preparative in Single LC-MS System,  <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00650_en_f326c9720e/an_01-00650-en.pdf"><strong>01-00650-EN</strong></a></li>
<li>High Purity Preparative Purification Enabled by UV/MS Trigger on LC-MS System,<a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00651_en_d88902f27d/an_01-00651-en.pdf"><strong>01-00651-EN.</strong></a></li>
</ol>
<h4><strong>3. Shimadzu: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/65237_tn_sfc_infographic_a417cd5f28.pdf">Rewriting the Book on Supercritical Fluid Chromatography</a></strong></h4>
<ul>
<li>Technical Note</li>
</ul>
<p>Fluids become supercritical when they are highly compressed and take on the properties of both a liquid and a gas. Supercritical carbon dioxide (CO₂) can be used as the mobile phase in supercritical fluid chromatography (SFC). Until recently, this technique had been overlooked in favor of high-performance liquid chromatography (HPLC) and gas chromatography (GC). However, new developments in the field have demonstrated that SFC can be a highly cost-effective, accurate and sensitive solution capable of outperforming HPLC and GC. In light of these new technologies, it’s time to reshape the field of chromatography with SFC.</p>
<h4><strong>4. Agilent Technologies: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_size_exclusion_chromatography_1290_infinity_ii_bio_5994_7664en_agilent_585512a949.pdf">Advanced SEC-MALS Analysis of Monoclonal Antibodies with the Agilent 1260 Infinity II Multi-Angle Light Scattering Detector</a></strong></h4>
<ul>
<li>Application Note</li>
</ul>
<p><strong>Abstract:</strong> Size exclusion chromatography (SEC) in combination with multiangle light scattering (MALS) detection enables accurate and reliable separation and molecular weight (MW) determination of monomer and aggregate protein species. The Agilent 1260 Infinity II Multi-Angle Light Scattering detector coupled to the Agilent 1290 Infinity II Bio LC System offers the possibility to measure the intensity of the scattered light at multiple angles to gain more precise absolute MW for the analysis of biopharmaceuticals. The 1290 Infinity II Bio LC is specially designed for conditions used in biochromatography with a completely iron-free flow path and a high tolerance to salt-based buffers such as those used in SEC.</p>
<p><strong>Conclusion:</strong> The possibility of using a MALS detector with 20 angles enables highly precise calculations of absolute molar mass. The MALS detector shows higher sensitivity than UV detection especially for higher protein aggregates, adding valuable information to a conventional SEC setup. The smaller angles allow the determination of higher molecular mass aggregates with high sensitivity and precision. The excellent resolution of the Agilent AdvanceBio SEC 300 Å column enables clear separation of monomer, dimer, higher aggregates, and mAb fragments, leading to unique MW determinations for the separated peaks. The combination of the Agilent 1290 Infinity II Bio LC System with the Agilent 1260 Infinity II Multi-Angle Light Scattering detector provides a complete biocompatible flow path to ensure trusted results for challenging solvent conditions or iron-reactive samples.</p>
<h4><strong>5. Agilent Technologies: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/te_evaluation_rohs_compliant_ion_injectors_5994_7613en_agilent_1dfe200fad.pdf">Evaluation of Agilent RoHS-Compliant LC/MS Ion Injectors</a></strong></h4>
<ul>
<li>Technical Note</li>
</ul>
<p><strong>Introduction:</strong> Recently, governments across the globe have started to reduce the use of hazardous materials in electronic components. Specifically, the Restriction of Hazardous Substances in Electrical and Electronic Equipment (RoHS) Directive in the European Union has been driving this effort. The Agilent resistive ion injector has been updated to a leaded glass–free, RoHS-compliant-without-exemption part for use in Agilent LC/MS quadrupole instruments that do not have an ion funnel. The ion injector acts to pull desolvated ions from the desolvation chamber into the MS (Figure 1). In doing so, the ions are culminated into a single, narrow beam, allowing the MS to separate the ions according to their mass-to-charge ratio (m/z). The ion injector is resistive, which allows for fast polarity mode switching, and has metal contacts at either end for electrical connection to the MS. In this technical overview, the performance of the Agilent RoHS ion injectors was compared to that of their respective legacy ion injector models (Table 1).</p>
<p><strong>Conclusion:</strong> In this technical overview, the Agilent RoHS-compliant ion injectors were compared to their respective Agilent legacy ion injectors. The studies presented in this technical overview demonstrate equivalent functionality between the legacy and RoHS ion injector models. Specifically, tune performance was shown to be equivalent between RoHS and legacy ion injectors, and lifetime performance under acidic and basic conditions was also shown to be comparable between RoHS and legacy ion injections, including at high mass on the Agilent 6545XT AdvanceBio LC/Q-TOF. Moreover, IDLs and S/N were within specifications on multiple of the tested RoHS ion injectors. Furthermore, the RoHS ion injectors held up to 10,000 injections of bovine urine and maintained tune criteria throughout, demonstrating excellent robustness. Finally, no differences in performance were observed on two different MS platforms when running target pharmaceuticals, both in neat samples and diluted human urine. In conclusion, the RoHS Agilent FS and Agilent Ultivo and MSD iQ ion injectors are suitable drop-in replacements for the legacy Agilent FS and Agilent Ultivo ion injectors.</p>
<h4><strong>5. Waters Corporation: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/2024_pegs_hanna_peptidworkskit_ce7f104856.pdf">PEGS: SIMPLE AND AUTOMATABLE SAMPLE PREPARATION FOR TRYPTIC PEPTIDE MAPPING WITH THE PEPTIDEWORKS KIT</a></strong></h4>
<ul>
<li>Poster, PEGS 2024</li>
</ul>
<p><strong>NTRODUCTION</strong></p>
<p>Reliable day-to-day sample preparation is a  critical factor in generating an effective  peptide mapping method. Sample  preparation for peptide mapping can result in  method-induced peptide modifications, over-  or under-digestion of the protein, and  autolysis of the proteolytic enzyme, each of  which complicate data analysis and  interpretation.</p>
<p>Waters’ PeptideWorkTryptic Protein  Digestion Kits deliver fast and reliable  sample preparation for routine peptide  mapping of therapeutic proteins. The sample  preparation kit is centered around  RapiZymTrypsin, Waters’  homogeneously methylated, recombinant  trypsin. RapiZyme Trypsin enables speed,  digestion fidelity, and low levels of trypsin-  derived peptides through its autolysis  resistance, purity, and high activity.  RapiZyme Trypsin can be used at high  concentrations to achieve faster digestions  without requiring high temperatures or  sacrificing digestion completeness.</p>
<p><strong>CONCLUSION</strong></p>
<ul>
<li>Comprehensive kit and protocol for  preparation of tryptic protein digests </li>
<li>Automatable preparation of 24 samples in  under 2.5 hours</li>
<li>Reproducible sample prep without  sacrificing digestion completion or  generating high levels of method-induced  peptide modifications </li>
<li>93% reduction in missed cleavages and  55% reduction in non-specific cleavages  compared to a leading immobilized trypsin  digest kit</li>
<li>Suitable for peptide mapping of ADCs</li>
</ul>
<h4><strong>6. Thermo Fisher Scientific: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_002998_ic_ms_integrion_isq_ec_environmental_water_an002998_na_en_b7542bd84c.pdf">Determination of selenite and selenate in environmental waters by ion chromatography-mass spectrometry (IC-MS)</a></strong></h4>
<ul>
<li>Application note</li>
</ul>
<p><strong>Keywords:</strong> Single quadrupole mass spectrometer, Dionex Integrion HPIC, Dionex IonPac AS11-HC column, ISQ-EC, ADRS 600 suppressor</p>
<p><strong>Goal:</strong> To develop a method to determine selenite and selenate in spiked environmental water by coupling ion chromatography (IC) with single quadrupole mass spectrometry.</p>
<p><strong>Conclusion:</strong> An IC-MS method that enables the determination of selenium species in simulated contaminated environmental waters was developed. The reagent-free ion chromatography system provides excellent reproducibility, yielding exceptional quantification accuracy and consistently reliable results. Recoveries for selenite and selenate in the three water samples ranged between 90–110%, with a method LOD of 4 and 2 µg/L for selenite and selenate, respectively. This method is helpful for highly selective screening of selenium species at µg/L levels in environmental waters. Although not with the same sensitivity as IC-ICP-MS, it offers the advantage of selectively identifying selenium species through their m/z ratio. Moreover, this method enables determining whether drinking water exceeds the EPA&#39;s outlined MCL of 50 ppb for selenium contamination.</p>
<h4><strong>7. Thermo Fisher Scientific: <a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_002998_ic_ms_integrion_isq_ec_environmental_water_an002998_na_en_b7542bd84c.pdf">Size-exclusion chromatography of adeno-associated viruses with the SurePac Bio 550 SEC MDi column</a></strong></h4>
<ul>
<li>Application note</li>
</ul>
<p><strong>Keywords:</strong> Biopharmaceutical, size-exclusion liquid chromatography, SEC, adeno-associated virus, AAV, monomeric capsids, high molecular weight species, HMWS, 3 µm SurePac Bio 550 SEC MDi column, monodisperse</p>
<p><strong>Key benefits</strong></p>
<ul>
<li>High-resolution separation of monomeric AAV and aggregates by size-exclusion liquid chromatography within 4 minutes</li>
<li>Utilizing UV and fluorescence detection for easy and conclusive confirmation of peak identities</li>
<li>Resolution of aggregate and monomer peaks preserved even for injection of large volumes</li>
<li>Consistent lot-to-lot and column-to-column performance</li>
</ul>
<p><strong>Goal:</strong> To demonstrate baseline separation of adeno-associated virus (AAV) monomeric capsids and high molecular weight species (HMWS) using a 4.6 × 150 mm sizeexclusion liquid chromatography column packed with 3 µm monodisperse silica particles with a 550 Å pore size.</p>
<p><strong>Conclusions</strong></p>
<ul>
<li>The SurePac Bio 550 SEC MDi column delivers baseline resolution of AAV monomer and aggregate peaks in less than 4 minutes.</li>
<li>The SurePac Bio 550 SEC MDi column consistently delivers reliable separation of AAV across different lots.</li>
<li>Careful optimization of flow rate, sample loading amounts, and buffer conditions is crucial for achieving accurate and consistent results in SEC AAV separation.</li>
<li>The SurePac Bio 550 SEC MDi column outperforms other commercially available columns in AAV analysis, displaying advantages such as sharper peaks, improved resolution, and lower operational pressure</li>
</ul>


News from LabRulezLCMS Library - Week 31, 2024

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week 31, 2024? Check out new documents from the field of spectroscopy, especially ICP/MS techniques!</strong></p>
<blockquote>
<h4>👉 <a href="https://icpms.labrulez.com/library"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT ICPMS AND RELATED TECHNIQUES</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> libraries.</p>
</blockquote>
<h4><strong>1. Metrohm: <a href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_ra_009_download_d7cc0b953a.pdf">Comparison of SPELEC RAMAN and standard Raman microscopes</a></strong></h4>
<ul>
<li>application note</li>
</ul>
<p><strong>Larger laser spot size can provide representative results with a single measurement</strong></p>
<p>Standard Raman microscopes are traditionally used to perform Raman measurements or Raman spectroelectrochemical experiments when they are coupled with electrochemical equipment. Raman spectra collected with confocal microscopes allows the characterization of very small areas. However, these instruments can exhibit several limitations. SPELEC RAMAN, a new generation of spectroscopic and spectroelectrochemical instruments, offers powerful and interesting features to overcome these issues. In this Application Note, a detailed comparison is made between the main features of a standard Raman instrument and the SPELEC RAMAN by analyzing the results obtained with both instruments.</p>
<p><strong>CONCLUSION</strong></p>
<p>SPELEC RAMAN exhibits a number of competitive advantages over standard Raman microscopes. For instance, the spot size of SPELEC RAMAN allows the characterization of a large area in a single experiment, obtaining representative results with only one measurement. The versatility of the Raman probe is demonstrated since it can be used with different cells. The portability as well as the simplicity of SPELEC RAMAN facilitate the performance of optical experiments. It also ensures the synchronization of electrochemical and optical signals in case Raman spectroelectrochemical measurements are carried out. In addition, DropView SPELEC software allows realtime acquisition of the data and the performance of operando measurements. The easy data treatment and analysis of the results is done using one-click tools.</p>
<h4><strong>2. Shimadzu: <a href="https://icpms.cz/labrulez-bucket-strapi-h3hsga3/c103_e421_5c783b2e76.pdf">FTIR Talk Letter (vol. 42)</a></strong></h4>
<ul>
<li>other</li>
</ul>
<p><strong>Table of content</strong></p>
<ul>
<li>Development of Dual Functional Catalytic Materials for CO2 Capture and Selective Hydrogenation and Mechanistic Elucidation of High CO Selectivity Using FTIR Spectroscopy | 02 </li>
<li>Configuration of the AIRsight Infrared Raman Microscope | 06</li>
<li>Notes on Infrared Spectral Analysis – Aliphatic Saturated Hydrocarbons (Paraffins) | 10</li>
<li>Fourier Transform Infrared Spectrophotometers &quot;IRSpirit-X Series&quot; | 16</li>
</ul>
<h4><strong>3. Agilent Technologies: <a href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/ab_tissue_metals_la_icp_ms_8900_5994_7575en_agilent_8aa962e737.pdf">Study of Localization of Endogenous Metals on Thin Tissue Sections by LA-ICP-MS</a></strong></h4>
<ul>
<li>Application Brief</li>
</ul>
<p><strong>Multi-element analysis of biological samples using laser ablation with an Agilent ICP-MS</strong></p>
<p><strong>LA-ICP-MS provides bioimaging and metallomics data</strong></p>
<p>Bioimaging and metallomics are complementary fields of study that explore the role of analytes and metals in biological systems. Bioimaging visualizes the presence or absence of certain tissues, cells, and low molecular weight compounds while metallomics seeks to understand the functions and roles of metals in biological structures. Analytical techniques such as liquid-chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS), single cell-ICP-MS, and laser ablation (LA) ICP-MS have been used for protein quantification1 , single cell analysis2 , and bioimaging, respectively.</p>
<p>LA-ICP-MS can provide detailed information about the localization of elements on the surface of solid samples, as demonstrated in this study for the investigation of the distribution of metals on thin sections of biological samples. </p>
<h4><strong>4. Thermo Fisher Scientific: <a href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_002778_tea_icap_pro_series_fertilizer_an002778_em_en_8c4a437262.pdf">Combining full spectrum read-out and innovative automation capabilities for the robust and fast analysis of major and trace elements in fertilizers using ICP-OES</a></strong></h4>
<ul>
<li>Application note</li>
</ul>
<p><strong>Keywords:</strong> ICP-OES, robustness, method validation, sensitivity, Step Ahead, limits of quantitation, analytical testing, toxic elements</p>
<p><strong>Goal:</strong> To demonstrate the capabilities of the Thermo Scientific™ iCAP™ PRO XP ICP-OES Duo instrument, in conjunction with the Thermo Scientific™ iSC-65 Autosampler, for the fast and robust elemental analysis of fertilizers.</p>
<p><strong>Conclusions:</strong> This application note presents a fast and optimized method for the quick, accurate, and robust analysis of major and trace elements in fertilizers using the iCAP PRO XP ICP-OES Duo instrument and the iSC-65 Autosampler. The highlights of the study are listed below:</p>
<ul>
<li>A quick and simple method with a run time of 36 s per sample for all target elements in fertilizers has been developed. This has been achieved using the excellent sensitivity in radial mode and the fast data acquisition capabilities of the iCAP PRO XP ICP-OES Duo instrument using the iFR mode. Due to the fully simultaneous acquisition across the full optical spectrum, an additional exposure can be eliminated, offering a 50% reduction of the time required for data acquisition. </li>
<li>The use of the Step Ahead feature available on the iSC-65 Autosampler compliments the speed of the iCAP PRO XP ICP-OES Duo instrument and the chosen settings in this method by enabling rinsing parallel to data acquisition. The combination of both provides the opportunity to reduce sample turnover times further by almost 30% compared to a standard setup.</li>
<li>The method allows achievement of the typically required limits of quantification for commonly regulated elemental contaminants in fertilizers—such as As, Cd, Cr, Hg, Ni, and Pb—with respect to the criteria prescribed in the Regulation (EU) 2019/1009. At the same time, it is capable of covering a large linear dynamic range of 0.01–20 mg·kg-1 for trace elements and 1–1,500 mg·kg-1 for elements present in higher concentrations. If the total concentration of Cr is found to be higher than the specified limit, then a speciation technique may be used to determine the amount of Cr (VI) present in the samples. The concept of the application can be easily extended to other sample types, such as soil or wastewater analysis. ICP-MS instruments can also benefit from single mode measurement and the high-speed capabilities of the iSC-65 Autosampler.² </li>
<li>Continuous, uninterrupted measurements of &gt;5 hours covering more than 500 unknown samples proves the accuracy and unmatched robustness of the developed method, which makes the iCAP PRO XP ICP-OES Duo instrument an ideal choice for high productivity analytical testing laboratories.</li>
</ul>


News from LabRulezICPMS - Library Week 31, 2024

<p><strong>In the 2 weeks from August 12, the following webinars await you in the field of gas / solid phase and especially Raman, FTIR, GC and GC/MS.</strong></p>
<blockquote>
<h4><a href="https://gcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Agilent Technologies: Get the Most Out of Your MS: Novel Column Chemistry Raises the Bar on Sensitivity and Data Accuracy</strong></h5>
<ul>
<li>Tu, 13.8.2024 17:00 CEST</li>
<li><a href="https://gcms.labrulez.com//webinar/3908">Registration</a></li>
</ul>
<p>We’ll examine how column attributes like bleed, thermal stability, and inertness can translate into practical data quality improvements.</p>
<h5><strong>2. Phenomenex: Advancing Food Testing Using New and Unique GC Column Selectivities</strong></h5>
<ul>
<li>Tu, 13.8.2024 19:00 CEST</li>
<li><a href="https://gcms.labrulez.com//webinar/4161">Registration</a></li>
</ul>
<p>Learn about important applications in food testing and current trends. Recieve an overview of available new column selectivity, and relevant applications.</p>
<h5><strong>3. Shimadzu Corporation: Elevating Pharma 4.0: Harnessing CaliberLIMS For Digital Excellence</strong></h5>
<ul>
<li>We, 14.8.2024 08:30 CEST</li>
<li><a href="https://gcms.labrulez.com//webinar/4163">Registration</a></li>
</ul>
<p>CaliberLIMS is a robust, scalable, and reliable platform designed to meet the demands of modern laboratories aiming for Pharma 4.0.</p>
<h5><strong>4. Agilent Technologies: End Consumables Counting Chaos: Track and Trend for Timely GC Maintenance</strong></h5>
<ul>
<li>Th, 15.8.2024 17:00 CEST</li>
<li><a href="https://gcms.labrulez.com//webinar/4209">Registration</a></li>
</ul>
<p>Attend this GC Live session to learn how the latest GC instruments can track historical maintenance events and display trend plots for easy interpretation.</p>
<h5><strong>5. Agilent Technologies: UV-VIS-NIR in Battery Development</strong></h5>
<ul>
<li>Th, 15.8.2024 20:00 CEST</li>
<li><a href="https://gcms.cz/webinar/3909">Registration</a></li>
</ul>
<p>Agilent&#39;s portfolio of instruments offers the high performance and flexible accessories needed for pioneering new applications.</p>
<h5><strong>6. Agilent Technologies/Markes International: Environmental Air Monitoring: Methodologies, Regulations, and Market Trends</strong></h5>
<ul>
<li>Tu, 20.8.2024 10:30 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/4187">Registration</a></li>
</ul>
<p>An in-depth webinar on environmental air quality monitoring application solutions, organized by Agilent Technologies in collaboration with Markes International.</p>


Webinars LabRulezGCMS Week 33-34/2024

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Plastics

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Presentation

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Various

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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LECO Training Course CS-744/844 Series & ON/ONH-736/836 Series

SCIEX at SOFT/TIAFT

Waters at SOFT/TIAFT 2026

Automatic ICP-OES Analysis of Lubricating Oils Using SPECTRO GENESIS DSOI and ACAROS Automation

Detection and Identification of Microplastics in Water and Other Matrices Using the Agilent Laser Infrared Spectroscopy Microscope

Unlocking New Analytical Possibilities with Waters APGC Solutions