<p><strong>In the week of January 13, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Waters Corporation: Light scattering-based solutions for excipients and advanced formulations</strong></h5><div><div><ul><li><div>Tu, 14.1.2025 05:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4545">Registration</a></div></li></ul></div><p>In this webinar, we will showcase case studies demonstrating how SEC-MALS and FFF-MALS are utilized to characterize, quantify, and deformulate advanced excipients and formulations.</p></div><h5><strong>2.&nbsp;Thermo Fisher Scientific: Well characterized biological products, challenging yet rewarding</strong></h5><div><div><ul><li><div>Tu, 14.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4578">Registration</a></div></li></ul></div><p>The presentation emphasizes the importance of developing innovative methods and technologies to support the ecosystem and enhance data workflows. It introduces the concept of a new facility, named "Concept".</p></div><h5><strong>3.&nbsp;Agilent Technologies: Getting the Most from Your Agilent HPLC Pump</strong></h5><div><div><ul><li><div>Tu, 14.1.2025 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4519">Registration</a></div></li></ul></div><p>We will focus on your Agilent HPLC pump. We will look at some of its capabilities and how to choose the right column for it. We will discuss best practices to help maximize up-time.</p></div><h5><strong>4.&nbsp;Restek Corporation: Incorporating ultrashort-chain compounds into the comprehensive analysis of PFAS</strong></h5><div><div><ul><li><div>We, 15.1.2025 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4569">Registration</a></div></li></ul></div><p>Join Dr. Shun-Hsin Liang, as he explores the simultaneous analysis of C1 to C14 PFCA and PFSA, along with other PFAS types, using two alternative chromatographic approaches.</p></div><h5><strong>5.&nbsp;SCIEX: Unlocking precision: nitrosamine and NDSRI analysis</strong></h5><div><div><ul><li><div>Th, 16.1.2025 10:30 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4682">Registration</a></div></li></ul></div><p>We invite you to join our webinar on nitrosamine and NDSRIs, specifically examining their analysis and implications in the pharmaceutical industry.</p></div><h5><strong>6.&nbsp;Thermo Fisher Scientific: The merger of automated LC fractionation &amp; in-depth characterization</strong></h5><div><div><ul><li><div>Th, 16.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4579">Registration</a></div></li></ul></div><p>Dr. Kristensen discusses how the shift from traditional data handling methods to cloud solutions has revolutionized their workflow, enabling access to vast amounts of data from anywhere.</p></div><h5><strong>7.&nbsp;Waters Corporation: Mastering the Quantitative Bioanalysis of Large Peptides and Proteins: A Case Study of GLP-1 Receptor Agonist Semaglutide in Plasma</strong></h5><div><div><ul><li><div>Th, 16.1.2025 15:00 CEST</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4384">Registration</a></div></li></ul></div><p>We present new data demonstrating a comprehensive and streamlined approach to optimized LC-MS/MS quantification of peptides, addressing the common challenges.</p></div><h5><strong>8.&nbsp;Waters Corporation:</strong> <strong>Method Validation</strong></h5><div><div><ul><li><div>Th, 16.1.2025 19:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4669">Registration</a></div></li></ul></div><p>We´ll discuss practical guidance around accuracy, precision, limit of quantitation and limit of detection and next we´ll walk you through an Empower CDS tool.</p></div>

Webinars LabRulezLCMS Week 3/2025

<p><strong>In the week from January 13, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Anton Paar: How Sweet It Is: Innovations and Automation in Sugar and Chocolate Analysis</strong></h5><div><div><ul><li><div>Tu, 14.1.2025 18:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4448">Registration</a></div></li></ul></div><p>This webinar describes a comprehensive approach to analyzing sugar, confectionary products, and chocolate.</p></div><h5><strong>2.&nbsp;Shimadzu Corporation: Elemental Analysis: Energize the Future with Advanced Battery Testing Solutions</strong></h5><div><div><ul><li><div>Tu, 14.1.2025 19:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4668">Registration</a></div></li></ul></div><p>Innovative techniques for overcoming unique matric-specific challenges for analyzing black mass, electrolyte solutions, anode materials, and cathode materials by ICP-AES, TOC, and X-ray fluorescence.</p></div><h5><strong>3.&nbsp;Agilent Technologies: Automating the Workflow for the Analysis of Soils by ICP-OES. Less Work, More Flow</strong></h5><div><div><ul><li><div>Tu, 14.1.2025 21:30 CEST</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/3770">Registration</a></div></li></ul></div><p>ADS2 automates lab workflows, from calibrators to smart dilutions. Faster, smarter, and more accurate.</p></div><h5><strong>4.&nbsp;Agilent Technologies: Agilent 8700 LDIR Workflows</strong></h5><div><div><ul><li><div>Th, 16.1.2025 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/4637">Registration</a></div></li></ul></div><p>The two main worklflows of the LDIR will be discussed and demonstrated.</p></div>

Webinars LabRulezICPMS Week 3/2025

A fast thermal desorption unit for micro thermal desorption tubes, Part I: Development of the system and proof of concept measurements with hyper-fast gas chromatography

Understanding the fundamentals of the on-off retention mechanism of oligonucleotides and their application to high throughput analysis

Can NMR-HetCA be a Reliable Prediction Tool for the Direct Identification of Bioactive Substances in Complex Mixtures?

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezGCMS Library in the week of 13th January 2025? Check out new documents from the field of the gas phase, especially GC and GC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT GCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications and other documents by <strong>Agilent Technologies</strong>, <strong>Thermo Fisher Scientific</strong>,<strong> </strong>and<strong> Shimadzu</strong>!</p><h4><strong>1. Thermo Fisher Scientific: </strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_7000_mrm_toxicology_asms_2024_wp810_en_agilent_b9f153ff37.pdf"><strong>HERAtech Laboratories satisfies growing water-analysis demand and reduces costs using workflow automation</strong></a></h4><ul><li>Case study</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/paper/28639"><strong>Full PDF for download</strong></a></li></ul><p><i>“Automation is fundamental because thirteen analysts are not enough to cope with our increase in samples. The automated approach we use allows for method consolidation with the analysis of PAHs and pesticides in a single GC run. Additionally, the possibility to perform sample extraction automatically, followed by online GC-MS/MS analysis, offers a significant reduction of labor spent and a significant reduction in solvent consumption.”</i></p><p><i><strong>Dr. Paolo Morelli</strong>, Laboratory Manager, HERAtech S.r.l., Sasso Marconi (BO), Italy</i></p><p><i>“It’s necessary to evaluate every possible marginal gain on every single process in the workflow to remove any bottlenecks. The bottleneck that Thermo Fisher Scientific has helped us solve is huge for sample preparation for pesticides and in particular for PAHs by eliminating manual SPE.”</i></p><p><i><strong>Dr. Paolo Morelli</strong>, Laboratory Manager, HERAtech S.r.l., Sasso Marconi (BO), Italy</i></p><blockquote><h5><strong>Analytical system used at HERAtech for automated determination of pesticides and PAHs in water samples</strong></h5><ul><li>TriPlus RSH SMART autosampler</li><li>Thermo Scientific™ TRACE ™ 1610 Series gas chromatograph with Thermo Scientific™ iConnect™ Programmable Temperature Vaporizing (PTV) injector module for large-volume injection</li><li>Thermo Scientific™ TSQ™ 9610 triple quadrupole mass spectrometer</li></ul></blockquote><p><i>“The key aspect of this automated solution is an analytical system capable of carrying out automatically, in a fully unattended way, all the extraction phases of the LLE protocol using microvolumes.”</i></p><p><i><strong>Dr. Paolo Morelli</strong>, Laboratory Manager, HERAtech S.r.l., Sasso Marconi (BO), Italy</i></p><p><i>“We made significant investments focused on automation in terms of instruments that allow us to analyze more analytes in one run. In particular, we aimed to consolidate different methods into a single method and provide as many analytical measurements as possible with a single machine. Our goal was simultaneous analysis of 70 analytes including pesticides and PAHs, with quantification limits between 0.0005 μg/L (0.5 ng/L) and 0.02 μg/L.”</i></p><p><i><strong>Dr. Paolo Morelli</strong>, Laboratory Manager, HERAtech S.r.l., Sasso Marconi (BO), Italy</i></p><p><i>“Only two solvents are necessary to perform automated DLLME, and the much lower volumes offer significant cost savings. The sample volume required is much lower, reducing logistic costs of transportation and storage.”</i></p><p><i><strong>Dr. Paolo Morelli</strong>, Laboratory Manager, HERAtech S.r.l., Sasso Marconi (BO), Italy</i></p><h4><strong>2. Agilent Technologies/ASMS: A Triple Quadrupole GC/MS MRM Database for Forensic and Toxicological Workflows</strong></h4><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/paper/27204"><strong>Full PDF for download</strong></a></li></ul><p>Systematic toxicological analysis in forensic investigation demands continuous adaptability to an ever-evolving toxicant landscape.</p><p>The three main challenges are:</p><ol><li>low concentrations of the toxicants</li><li>an ever-growing number of analytes to be monitored and quantitated</li><li>the limitations in obtaining analytical standards for every chemical.</li></ol><p>For the volatile compounds, gas chromatography/mass spectrometry (GC/MS) is the method of choice when analyzing forensic drugs and toxicants [1, 2]. GC/MS <strong>forensic toxicological workflow</strong> greatly benefits from <strong>selectivity</strong> and <strong>sensitivity</strong> of multiple reaction monitoring (MRM) approach enabled with triple quadrupole GC/MS (GC/TQ).</p><p>The aim of this work was to develop an <strong>MRM database</strong> to help toxicological researchers build screening and quantitation methods simplifying method development.</p><p>The database of MRM transitions for relevant toxicants was established and successfully applied to creating GC/TQ methods for analyzing real-world authentic samples with <strong>greater sensitivity and confidence</strong> than the conventional GC/MS approach. The database will be available for download starting July 2024.</p><h4><strong>3. Shimadzu: GC/GC-MS Glass Insert/Liner Selection Guide</strong></h4><ul><li>Guide</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/paper/26962"><strong>Full PDF for download</strong></a></li></ul><p>In a gas chromatograph, the sample is injected into the column via the injection unit. A glass insert / liner is used to vaporize the sample more efficiently, and to inject the vaporized sample into the column with no unnecessary dispersion. Additionally, using a glass insert reduces the extent to which contaminants such as high boiling point compounds from the sample contaminate the injection unit. Accordingly, selecting a glass insert appropriate for the objective heightens the accuracy of analysis and leads to more reliable results.</p><p>When selecting a glass insert, various selection standards are used depending on the application. For example, the following cases can be cited as selection standards for glass inserts.&nbsp;</p><ul><li>What type of injection unit is used? What sample injection method is used during analysis?</li><li>Is the glass insert filled with wool (glass wool)? What shape is the glass insert?</li><li>What are the dimensions of the glass insert?</li><li>Is surface treatment of the glass insert necessary?</li></ul><p>Glass inserts with a variety of specifications are commercially available to suit these varied selection criteria, so selecting a glass insert can be daunting.</p><p>This selection guide describes how to select an appropriate glass insert for the objective/application as well as points to consider in accordance with elements of the glass insert configuration. Additionally, this guide introduces glass inserts appropriate for your Shimadzu GC system.</p>

News from LabRulezGCMS Library - Week 3, 2025

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 13th January 2025? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications by <strong>Thermo Fisher Scientific,</strong> <strong>Shimadzu</strong>,<strong> </strong>a presentation by <strong>Agilent Technologies</strong>, a manual by <strong>Metrohm,</strong> and a poster from <strong>ASMS </strong>by <strong>Waters Corporation</strong>!</p><h4><strong>1. Thermo Fisher Scientific: High-resolution DIA proteomics workflow for single-cell samples on the Orbitrap Astral mass spectrometer</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/28638"><strong>Full PDF for download</strong></a></li></ul><p>Assess proteome coverage and sample throughput performance for single-cell samples using a library-free and library-based data-independent acquisition (DIA) method on the Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer.</p><h5><strong>Introduction</strong></h5><p>In recent years, there has been a shift to analyzing smaller sample quantities, driven by the interest in profiling the proteome of individual cells. This increased focus on LC-MS analysis of limited sample amounts, e.g., single cells, requires the highest possible sensitivity. At the same time, throughput and chromatographic performance must be preserved to generate sufficiently high-quality data from large data sets to draw meaningful conclusions. This trend toward analyzing smaller sample quantities reflects the need to understand the heterogeneous nature of biology through the dissection of complex systems into their individual parts or, specifically, individual cells. Traditional proteomics approaches read out the average of a sample while single-cell proteomics provides insights into the exact nature of each of the components in such samples.</p><p>The Orbitrap Astral mass spectrometer delivers a new standard for single-cell samples with deeper proteome coverage and faster throughput. It enables these capabilities through the synchronized acquisition of high-resolution and high-dynamic range full scan spectra with the Thermo Scientific™ Orbitrap™ mass analyzer and high speed and sensitivity MS/MS spectra with the Thermo Scientific™ Astral™ mass analyzer. The Thermo Scientific™ Vanquish™ Neo UHPLC system delivers maximum performance in terms of precise, low flow rates and gradient formation, as well as low injection volumes—an important benefit for single-cell proteomics and limited sample amounts.</p><p>The Thermo Scientific™ μPAC™ Neo HPLC columns with novel micro-pillar array deliver highly reproducible chromatographic separation and increased sensitivity with maximum resolution.<br>The Thermo Scientific™ FAIMS Pro Duo interface enhances sensitivity by removing background signals.</p><p>In this technical note, we present a single-cell proteomics workflow (Figure 1). To first evaluate sensitivity and quantitative precision and accuracy and linearity of the workflow, a dilution series from 5 ng to 50 pg of the Thermo Scientific™ Pierce™ HeLa protein digest standard was analyzed.</p><h4><strong>2. Shimadzu: Automatic Optimization of Gradient Conditions by AI Algorithm - Consecutive Optimization at Different Column Oven Temperatures</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/28642"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>The AI algorithm of LabSolutions MD can automatically optimize gradient conditions to greatly reduce labor of LC method development.</li><li>Gradient conditions are automatically optimized at different column oven temperatures consecutively.</li><li>Automatic optimization of gradient conditions can be applied not only to new method development, but also to existing method to efficiently improve resolution.</li></ul><h5><strong>Introduction</strong></h5><p>In the typical LC method development, the process begins with “preparation” which includes mobile phase preparation, column installation, and creation of analysis schedules, then the analysis is started. After that, the acquired data is analyzed and “preparation” for the subsequent analysis is carried out, followed by starting the next analysis again. The method development progresses by repeating these processes, but in addition to the significant time required to repeatedly create analysis schedules, expertise in chromatography is necessary to explore optimal conditions based on data analysis. In other words, typical method development requires “human intervention”. Therefore, eliminating human involvement and automating such method development processes would be desirable to improve labor efficiency. This article introduces an approach for automatically optimizing gradient conditions at different column oven temperatures consecutively, identifying the combination of temperature and gradient conditions that meet the resolution criteria.</p><h5><strong>Conclusion</strong></h5><p>Automatic optimization of gradient conditions using AI algorithm of LabSolutions MD was applied to a model sample (mixture of seven compounds of small molecule) at different column oven temperatures. As a result, the temperature and gradient conditions that met the resolution criteria were successfully explored. In method development, human intervention, such as analysis batch creation and data analysis, is required to optimize gradient conditions. LabSolutions MD can provide significant labor savings in this area. For more information on LabSolutions MD, please refer to the Technical Report <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/c190_e284_1ca86b48ea/c190-e284.pdf">“Efficient Method Development Based on Analytical Quality by Design with LabSolutions MD Software (C190-E284)”</a>.</p><h4><strong>3. Agilent Technologies: Optimize Your Polymer Analysis by Selecting the Ideal GPC/SEC Column</strong></h4><ul><li>Presentation</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/28578"><strong>Full PDF for download</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4481"><strong>Recorded webinar</strong></a></li></ul><p>&nbsp;</p><ul><li><strong>GPC/SEC Separation Mechanism</strong></li><li><strong>Conventional GPC/SEC Workflow</strong></li><li><strong>Considerations for GPC/SEC Column Selection (Key questions to ask)</strong><ul><li>What polymer are you analysing?</li><li>Which solvent (or solvents) is your polymer soluble in?</li><li>What is the expected molecular weight range of your polymer?</li><li>What is the requirement for your analysis or what would you like to improve about your existing GPC/SEC separation?<ul><li>Resolution is important</li><li>Reproducibility of sample chromatography and results</li><li>Speed of analysis or sample throughput is something to improve on</li></ul></li></ul></li><li><strong>Further considerations</strong><ul><li>Know the properties of the sample</li><li>Be familiar with the properties of the columns being considered</li><li>It is important to balance polarities for the sample, solvent, and column packing</li></ul></li><li><strong>Common Column Chemistries for GPC/SEC</strong></li><li><strong>Agilent Magic Triangles</strong></li><li><strong>Column Selection: Porosity</strong></li><li><strong>Individual Pore Versus MultiPorous Particle</strong></li><li><strong>Column Selection: Particle size</strong></li><li><strong>Columns for Low Polarity Organic Eluents</strong></li><li><strong>Columns for Medium Polarity Organic Eluents</strong></li><li><strong>Columns for High Temperature GPC/SEC</strong></li><li><strong>Columns for Fluorinated Organic Eluents</strong></li><li><strong>Columns for Aqueous Eluents: Neutral or weak Anions</strong></li><li><strong>Columns for Aqueous Eluents: Polycations and Strong Polyanions</strong></li><li><strong>Column Selection: Additional Considerations</strong></li><li><strong>Columns in Series</strong></li><li><strong>Wrongly Coupled Columns</strong></li><li><strong>Combination of Individual Pore Size Columns</strong></li><li><strong>Column Selection and Importance of Solvent Choice</strong></li><li><strong>Solvent Selection</strong></li><li><strong>Successful Solvent Choice</strong></li><li><strong>Eluent Modification in Organic GPC</strong></li><li><strong>Common Questions Around GPC/SEC Standards</strong></li><li><strong>Agilent Polymer Calibration Standard Offerings</strong></li><li><strong>Well Characterized Polymer Standards</strong></li><li><strong>Easivial Certificate of Analysis</strong></li><li><strong>Common Questions Around SEC Standards</strong></li><li><strong>Installation and Care of Your Column</strong></li><li><strong>Before Your Analysis</strong></li><li><strong>Column Selection – Additional considerations</strong></li><li><strong>System Tubing</strong></li><li><strong>Your GPC/SEC Toolbox</strong></li></ul><h4><strong>4. Metrohm: Installation Instruction for ProfIC Vario 7 Cation</strong></h4><ul><li>Manual</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/28584"><strong>Full PDF for download</strong></a></li></ul><p>The ProfIC Vario 7 Cation is a Professional IC Vario system with Inline Dilution and Inline Dialysis for the fully automatic determination of cations in high concentrations in matrices containing emulsions. This system enables users to dilute the sample and eliminate droplets of fat or organic solvents or particles prior to the analysis.</p><h4><strong>5. Waters Corporation: IMPROVED TISSUE HOMOGENIZATION AND SPE-BASED SAMPLE PREPARATIONS FOR THE QUANTITATIVE LC-MS ANALYSIS OF OLIGONUCLEOTIDE THERAPEUTICS</strong></h4><ul><li>Poster/ASMS</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/27580"><strong>Full PDF for download</strong></a></li></ul><p>Efficient extraction of drug analytes is a critical aspect of drug metabolism and pharmacokinetics (DMPK) studies. This has long applied to small molecules and still applies to the bioanalysis of oligonucleotides. Oligonucleotide drugs and their metabolites must be quantified within both biofluids and tissue samples. The newest of oligonucleotide drugs are both extensively modified and conjugated. These modified residues and conjugate moieties can complicate extraction recovery and reproducibility. In this work, we report several key insights on how to achieve improved extractions. Protocols for solvent-assisted proteinase K sample pre-treatments are investigated using a weak anion exchange (WAX) microplate-based solid phase extraction (SPE) device. Direct injection LC-MS quantitation is demonstrated for all three antisense oligonucleotides (ASOs).</p><h5><strong>CONCLUSIONS</strong></h5><ul><li>Selective extraction and elution of ASO oligonucleotides is feasible from multiple tissue types.</li><li>Extracted ASOs are amenable for either full scan mode analysis on benchtop small footprint TOF MS or MRM analysis by Triple Quad MS</li><li>Reproducible and repeatable data were collected even without the application of an internal standard</li><li>One novel aspect of the SPE eluate is that no further sample preparation is required for subsequent LC-MS analysis saving time and avoiding sample losses during drying and reconstitution</li><li>Eluted nucleic acid components can be readily analyzed by regular ion-pairing reversed phase chromatography and UV/MS-based detection and quantitation</li><li>This robust tissue extraction protocols for improved LC-MS based bioanalysis of ASO drugs was repeatable and highly reproducible across multiple batches of SPE sorbent, providing confidence in the suitability of the optimized protocols</li></ul>

News from LabRulezLCMS Library - Week 3, 2025

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week of 13th January 2025? Check out new documents from the field of spectroscopy/spectrometry and related techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT SPECTROSCOPY/SPECTROMETRY RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications by <strong>ALS Europe</strong>, <strong>Thermo Fisher Scientific,</strong> <strong>Agilent Technologies and</strong> <strong>Shimadzu</strong>!</p><h4><strong>1. ALS Europe:&nbsp;Isotope Ratio Analysis Solves Complex Problems</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/paper/28592"><strong>Full PDF for download</strong></a></li></ul><p>ALS Laboratories are one of the few in the world to offer commercial isotope ratio testing services not only for radiogenic systems (Sr, Nd, Pu, U) and light stable isotopes (Li, B, Si), but also for heavy stable elements such as Ag, Ca, Cd, Cu, Fe, Mg, Mo, Si, and Zn.</p><p>All of these isotope ratio tests can be used as a fingerprint to provide information about origin or geologica<strong>l age, and have potential to be used for tracing pollution and exposure sources.</strong></p><h5><strong>ALS Capabilities for Isotope Ratio Analysis</strong></h5><p>ALS experts have more than 30 years of research and commercial testing experience for stable isotope ratio analysis using both High Resolution Sector Field ICP-MS (ICP-SFMS) and Multi-Collector ICP-MS (MC-ICP-MS) instrumentation. Our expert team has made substantial contributions to the field of isotope ratio analysis with 170 peer-reviewed publications. ALS laboratories offer isotope ratio analyses for more than 20 stable and radiogenic isotopic systems in a variety of sample matrices with high precision, even where sample concentrations are very low.</p><h4><strong>2. Thermo Fisher Scientific: Highly sensitive and reliable analysis of distillate products as per ASTM Method D8110 using single quadrupole ICP-MS</strong></h4><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/paper/28389"><strong>Full PDF for download</strong></a></li></ul><p>The goal of this application note is to demonstrate the performance of an analytical method for routine analysis of typical samples relevant to the petrochemical industry, including starting materials such as crude oil but also products such as naphtha, with different boiling ranges using single quadrupole ICP-MS following the guidelines provided in ASTM Method D8110 Standard Test Method for Elemental Analysis of Distillate Products by Inductively Coupled Plasma Mass Spectrometry (ICP-MS).</p><h5><strong>Conclusions</strong></h5><p>The results obtained from the experiments described in this application note demonstrate the following:</p><ul><li>The proposed method and instrument set-up for elemental analysis of different petroleum products using the iCAP MSX ICP-MS represents a viable solution for laboratories performing oil and refinery product testing.</li><li>The combination of the sample introduction system together with a uniquely robust plasma generation system and a powerful CRC allows for sensitive analysis and comprehensive interference removal for all elements commonly analyzed in the industry. A common sample preparation method encompassing simple dilution of any products with either higher viscosity or lower boiling point in PremiSolv offers complete flexibility for the analysis of a variety of based petroleum sample matrices.</li><li>The use of KED with helium provided effective for removal of polyatomic interferences commonly observed in such challenging matrices, as was demonstrated by the detection limits and blank equivalent concentrations (BECs) achieved.</li><li>The method therefore allows the accurate and precise determination of a total of 28 elements in different kinds of distillate products, as was demonstrated by the results obtained in the spike recovery test.</li><li>If needed, other reactive gases can be used in a multi-mode analysis performed during a single aspiration of a sample, including alternative cell gases such as hydrogen. Thanks to the fast gas switching in the QCell CRC, the added time for switching cell gases is often negligible for the overall analysis time per sample.</li></ul><p>Taken together, the results obtained demonstrate that the proposed analytical method can be used in routine laboratories to achieve high-throughput, robust, and reliable analytical results with minimum downtime of the system.</p><h4><strong>3. Agilent Technologies: Improved Lab Efficiency with the Agilent 18-Cell Changer Accessory</strong></h4><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/paper/26590"><strong>Full PDF for download</strong></a></li></ul><p>Evaluation of an Agilent Cary 60 UV-Vis spectrophotometer workflow using the 18-cell changer accessory</p><h5><strong>Introduction</strong></h5><p>In laboratory settings, time is of the essence. The speed at which any experiment can be conducted is important, and any advantages that can improve speed are critical. It is equally important that analytical workflows are optimized for maximum instrument use and minimal operator interaction.</p><p>The Agilent Cary 60 UV-Vis spectrophotometer is a flexible, powerful, and reliable UV-Vis system, ideal for routine analysis. To further improve workflow efficiency, the Cary 60 UV-Vis can be equipped with the Agilent 18-cell changer accessory. The 18-cell changer can measure up to 18 cuvettes in sequence, without any user interaction.</p><p>This technical overview compares a Cary 60 UV-Vis workflow using the 18-cell changer accessory versus a single-cell holder accessory to evaluate differences in terms of analysis time, data quality, user interaction, ease of use, and efficiency.</p><h5><strong>Conclusion</strong></h5><p>In summary, the Agilent 18-cell changer accessory for the Agilent Cary 60 UV-Vis spectrophotometer provides a range of benefits to laboratories and technicians alike. Compared to a single-cell holder, it dramatically increases efficiency by reducing the time taken to complete experiments and limiting the need for operator interaction, all without compromising the quality of results.</p><h4><strong>4. Shimadzu: Sensitivity Evaluation and Example Analysis of Microscopic Targets with Thermoelectrically Cooled MCT Detector</strong></h4><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/paper/28644"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>A thermoelectrically cooled MCT detector can measure microscopic targets down to 25 μm in size without needing liquid nitrogen.</li><li>An MCT detector is compatible with transmission, reflection, and ATR method for analyzing a range of sample types.</li><li>For targets smaller than 25 μm, the standard T2SL detector can measure targets down to 10 μm in size with good sensitivity.</li></ul><h5><strong>Introduction</strong></h5><p>Infrared spectroscopy is extremely useful for qualitative analysis of organic compounds and some inorganic compounds. Infrared microscopy can be used for spectroscopic analysis of microscopic targets that are otherwise difficult to analyze. The AIMsight Infrared Microscope and the infrared mode of the AIRsight Infrared/Raman Microscope are equipped as standard with the T2SL detector, which can analyze microscopic targets down to 10 μm in size if it is cooled with liquid nitrogen. However, the difficulties associated with procuring and handling liquid nitrogen mean there is increasing demand for infrared spectroscopic analysis of microscopic targets without using liquid nitrogen.</p><p>The thermoelectrically cooled (TEC) MCT detector is a new optional detector in Shimadzu’s product lineup that can analyze microscopic targets down to 25 μm in size without liquid nitrogen. This Application News compares this new detector with existing detectors and presents two example analyses of microscopic targets using the new detector.</p><h5><strong>Conclusion</strong></h5><p>This Application News compares the sensitivity of the new TEC MCT detector with existing detectors and presents two example analyses performed using the TEC MCT detector. The comparisons show the TEC MCT detector, which operates without liquid nitrogen, can measure targets as small as 25 μm with good sensitivity, and it is compatible with reflection and ATR spectroscopy as well as transmission spectroscopy for analysis of a range of sample types.</p><p>The T2SL detector, which requires liquid nitrogen, is recommended when analyzing targets smaller than 25 μm or when using fewer scans to shorten the analysis time. However, the minimum target size for infrared spectroscopic analysis with the T2SL detector is 10 μm, so targets smaller than 10 μm, Raman spectroscopy with the AIRsight infrared/Raman microscope should be used.</p>

News from LabRulezICPMS Library - Week 3, 2025

<p><strong>In the week from January 20, the following webinars await you in the field of gas / solid phase and especially Raman, FTIR, GC and GC/MS.</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>4 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Agilent Technologies: All About USP 467 Residual Solvent: Regulatory and Application Updates</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/4513">Registration</a></div></li></ul></div><p>Recent changes in USP 467, latest trends &amp; applications in residual solvent analysis along with analytical workflow in order to get accurate &amp; reproducible results.</p></div><h5><strong>2. Agilent Technologies:</strong> <strong>Make Your GC lab more productive with OpenLab CDS</strong></h5><div><div><ul><li><div>We, 22.1.2025 10:30 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/4671">Registration</a></div></li></ul></div><p>The latest variant of OpenLab CDS. OpenLab CDS in sync with GC platform provides latest features familiarization to ensure your lab is productive, efficient and compliant.</p><h5><strong>3. LECO: Two-Dimensional Gas Chromatography Coupled with Electron and Chemical Ionization Mass Spectrometry for the Analysis of Aviation Fuels</strong></h5><div><div><ul><li><div>We, 22.1.2025 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/4688">Registration</a></div></li></ul></div><p>The identification of different types of unknown compounds in complex mixtures by using GCxGC coupled with TOF mass spectrometry using positive-ion mode EI and methane CI is discussed.</p></div></div><h5><strong>4. Agilent Technologies:</strong> <strong>Using LDIR for Advanced Microplastics Characterization</strong></h5><div><div><ul><li><div>Th, 23.1.2025 09:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/4683">Registration</a></div></li></ul></div><p>This session will highlight the advanced features of LDIR technology and its critical applications in analyzing microplastics within various matrices.</p></div><h5><strong>5.&nbsp;Waters Corporation: Empower Tips: Ask Me Anything About Navigating Data Governance - Risk and Vigilant Monitoring</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/4550">Registration</a></div></li></ul></div><p>We will explore critical aspects of data governance and critical thinking, insights and strategies to help navigate the current environment, evolving your approach and modernizing your way of working.</p></div>

Webinars LabRulezGCMS Week 4/2025

<p><strong>In the week of January 20, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over&nbsp;</p><p><strong>4 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Characterization and sequence mapping of large RNA and mRNA therapeutics using HRAM</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4580">Registration</a></div></li></ul></div><p>The presentation showcases the innovative partial T1 digest strategy developed at the University of Sheffield for sequencing mRNA therapeutics.</p></div><h5><strong>2.&nbsp;SelectScience/Agilent Technologies: From Start to Finish: How Agilent Can Support Better Decisions in IVT mRNA-Based Biopharmaceutical Analysis</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4674">Registration</a></div></li></ul></div><p>We will discuss how automated electrophoresis and LC/MS systems solutions from Agilent provide reliable insights for IVT mRNA workflows at many steps.</p></div><h5><strong>3.&nbsp;Phenomenex: LC Troubleshooting Quick Guide: Spot Problems Fast &amp; Fix Them Like a Pro!</strong></h5><div><div><ul><li><div>Th, 23.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4676">Registration</a></div></li></ul></div><p>In this webinar - General troubleshooting approach, HPLC troubleshooting steps and diagnosing tips and preventive maintenance and column care.</p></div><h5><strong>4.&nbsp;Thermo Fisher Scientific: State-of-the-art E&amp;L is not rocket science</strong></h5><div><div><ul><li><div>Th, 23.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4581">Registration</a></div></li></ul></div><p>Ulli Honester, a team leader from Solvias, presented on advancements in mass spectrometry for identifying extractables and leachables (E&amp;L).</p></div><h5><strong>5.&nbsp;Waters Corporation: Empower Tips: Ask Me Anything About Navigating Data Governance - Risk and Vigilant Monitoring</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4550">Registration</a></div></li></ul></div><p>We will explore critical aspects of data governance and critical thinking, insights and strategies to help navigate the current environment, evolving your approach and modernizing your way of working.</p></div><h5><strong>6.&nbsp;Evosep Biosystems: A beginner’s guide to Evosep 2025</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4548">Registration</a></div></li></ul></div><p>This webinar is your opportunity to gain a comprehensive understanding of the Evosep One’s key features and how it can seamlessly integrate into your current processes.</p></div><h5><strong>7.&nbsp;Shimadzu Corporation: ASTM D8421 and D8535: Cost Effective Methods for Rapid PFAS Testing in Wastewater and Soil</strong></h5><div><div><ul><li><div>Th, 23.1.2025 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4465">Registration</a></div></li></ul></div><p>Discover simplified standardized methods for PFAS analysis based on co-solvation as sample preparation.</p></div>

Webinars LabRulezLCMS Week 4/2025

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LabRulez

LECO Separation Science Workshop

Thermo Fisher Scientific at SOFT/TIAFT 2026

Bruker at SOFT-TIAFT 2026

The Next Frontier in Nitrosamines Testing: Detection and Risk Assessment in Packaging and IV Infusion Bags

From Cleared Tissue to Quantitative 3D Insights: Integrated Bruker and Translucence Biosystems Workflows for Whole-Organ Imaging

Membrane Technologies for Water and Food Sustainability