Improving IEX Technology and IEX-MS Applications for Domain Specific Charge Variant Analysis of mAb

Characterization and Monitoring of mAb Charge Variants via Online IEX-MS Samantha Ippoliti Waters MS User Meeting ASMS 2019 Atlanta, GA June 1, 2019 ©2019 Waters Corporation 1 Agenda 1. mAb CQA Characterization 2. Reverse Phase vs Ion Exchange Chromatography 3.…

[ APPLICATION NOTE ] Automated Analytical Cation-Exchange Chromatography Fraction Collection and Structural Characterization of Monoclonal Antibody Charge Variants Hua Yang, Bill Warren, and Stephan Koza Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ Cation-exchange separation on Charge variant levels…

[ APPLICATION NOTE ] Online IEX-MS of mAb Charge Variants Using a BioResolve SCX mAb Column, IonHance CX-MS pH Concentrates, and BioAccord System Samantha Ippoliti, Andrew Schmudlach, Matthew A. Lauber, and Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION…

Online IEX-MS Characterization and Monitoring of mAb Charge Heterogeneity Using an Optimized Cation Exchange Resin and Compact TOF Mass Spectrometer Samantha Ippoliti1, Qi Wang1, Ying Qing Yu1, Matthew A. Lauber1, Henry Shion1 1 Waters Corporation, Milford, MA, USA INTRODUCTION RESULTS…