Intact Analysis Using Hydrophobic Interaction - Agilent BioHPLC Columns Application Compendium

Application Note Biologics Research AdvanceBio HIC: a Hydrophobic HPLC Column for Monoclonal Antibody (mAb) Variant Analysis Using the Agilent 1260 Infinity II Bio-Inert LC Authors Andrew Coffey and Sandeep Kondaveeti Agilent Technologies, Inc. Abstract This Application Note describes the separation…

Sandeep kondaveetil1, Andy Coffey2, Priya Jayaraman1, Linda Llyod2, Suma Ramagiri1 Hydrophobic Interaction Chromatography- Native Mode Separation of Bio-Therapeutic Workflows 1. Agilent Technologies, Inc., Wilmington, DE USA 2. Agilent Technologies, Inc., Church Stretton, UK Experimental LC Conditions – mAb oxidized variant…

Agilent BioHPLC Columns Characterization of NIST Monoclonal Antibody Critical Quality Attributes Application Compendium Contents Agilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted from NISTmAb RM 8671 batch. Quality control (QC) testing is performed using Agilent LC-MS system. QC batch release test…

Why Use HILIC and HIC Chromatography? Theory and method development strategies Mohit Patel, PhD LC Applications Engineer October 8, 2024 1 10/08/2024 DE-001530 AGILENT CONFIDENTIAL Agenda 1 What is HILIC and when should you consider it? 2 How to approach…