LipidSearch 5.0: A New Software for Processing Data from Direct Infusion and LC-MS High Resolution Mass Spectrometry Based Lipidomics Workflows

2 3 MS /MS Software Utilizing Positive and Negative Ion n MS HCD and CID Spectra for Improved Lipid Identification David A Peake1, Reiko Kiyonami1, Daniel Gachotte2, Gavin E Reid3, Yasuto Yokoi4, and Andreas Hühmer1 1Thermo Fisher Scientific, San Jose,…

Reiko Kiyonami1, David Peake1, Devin Drew1, Xuefei Sun2, Xiaodong Liu2, and Ken Miller1 1 Thermo Fisher Scientific, San Jose, CA, USA; 2Thermo Fisher Scientific, Sunnyvale, CA, USA Key Words C30 column, Q Exactive HF, high resolution, accurate mass, Orbitrap mass…

Higher Resolution LC-MS and MS-MS Analysis of Lipid Extracts Using Benchtop Orbitrap-based Mass Spectrometers and LipidSearch Software David A Peake,1 Yasuto Yokoi, 2 Reiko Kiyonami1 and Yingying Huang1 1 Thermo Fisher Scientific, San Jose, USA; 2Mitsui Knowledge Industry, Tokyo, JAPAN…

Results: Fatty acids comprising the molecular phospholipid species were unambiguously identified. ne Proteome Discoverer software is a node-based workflow engine and study management 55 platform for analysis of mass spectrometry-based proteomics datasets. The latest released version 2.1 fully supports isotopically-labeled…