Automated High-Throughput Clone Screening of Therapeutic Antibodies: From Automated IdeS Digestion to Intact Fc/2-Glycosylation Analysis by MALDI-TOF

Automated High-Throughput Oligo QC: From Automated Sample Preparation to MALDI Spectra Assessment Synthetic oligonucleotides find a variety of applications in molecular biology. Primers for instance are used for DNA sequencing and amplification. Mass spectrometry is a well-established technology for quality…

Extensive LC-Top-Down MS Sequence Confirmation of the NISTmAb Reference Material 8671 Characterization of therapeutic antibodies aims on primary sequence validation, localization of modifications such as glycan profiles and the determination of disulfide bond status of the target molecule. Introduction Typically,…

Application Note Automating Rapid High-Throughput LC-MS mAb Subunit Screening of Microbioreactor Cell Culture Samples Alireza Aghayee, Yamin Htet, Stephan M. Koza, Lindsay Morrison, Henry Shion, Ying Qing Yu Waters Corporation Abstract Monoclonal antibodies (mAbs) remain one of the fast-growing classes…

Label-free compound profiling in K562 leukemia cells by MALDI-TOF mass spectrometry using heme B as response marker Bruker’s very fast rapifleX MALDI PharmaPulse solution has been successfully used in pharmaceutical research for compound screening in various cell-free biochemical assays that…