Overcoming nonspecific binding in liquid chromatography: enhancing assay sensitivity, accuracy, and reproducibility in peptide/protein workflows

[ WHITE PAPER ] Achieving Maximum Protein and Peptide Recovery, Sensitivity, and Reproducibility using QuanRecovery Vials and Plates Moon Chul Jung Waters Corporation, Milford, MA, USA WHAT IS NON-SPECIFIC BINDING AND WHY IS IT IMPORTANT IN QUANTITATIVE ASSAYS? All successful…

FACTORS THAT INFLUENCE THE RECOVERY OF HYDROPHOBIC PEPTIDES DURING LC-MS SAMPLE HANDLING Moon Chul Jung, Kim Haynes, Markus Wanninger, Waters Corporation, 34 Maple Street, Milford, MA 01757 INTRODUCTION RESULTS Successful protein and peptide quantitation using LC-MS requires continuous attention to…

HPLC Vials Sample loss starts at the surface— minimize non-specific binding and maximize recovery. Did you know a significant amount of your sample could be left stuck inside your vial? When it comes to non-specific binding (NSB), choosing the right…

FACTORS THAT INFLUENCE THE RECOVERY OF HYDROPHOBIC PEPTIDES DURING LC-MS SAMPLE HANDLING Moon Chul Jung and Thomas H. Walter, Waters Corporation, 34 Maple Street, Milford, MA 01757, USA INTRODUCTION RESULTS AND DISCUSSION A sensitive and reproducible LC-MS analysis of biomolecules…