On-demand Online Reduction of Intact Antibodies for Automated Workflows on SampleStream coupled to a timsTOF

How to Determine Glycan Profiles of Biopharmaceuticals from Peptide Mapping Data A new and sensitive approach combining BioPharma Compass, PASEF and VIP-HESI Abstract Glycosylation is a common critical quality attribute (CQA) of therapeutic proteins and needs to be characterized during…

Recombinant SARS-CoV-2 Receptor Binding Domain: Comprehensive Top-Down Sequence Confirmation, Curation and O-Glycosylation Site Determination Know your SARS-CoV-2 antigen structure before it´s use in research and diagnostics Abstract The COVID pandemic dramatically influences our life and the demand remains very high…

Next-generation MALDI top-down sequencing of protein biotherapeutics – expanding the scope of timsTOF technology Next-generation MALDI top-down sequencing (MALDI-TDS) improves top-down sequence analysis of biopharmaceutical proteins, as demonstrated in this note for adalimumab subunits, recombinant SARS-CoV-2 S-glycoprotein RBD expression products,…

Application Note Biopharma/Pharma Automated Monoclonal Antibody Subunit Analysis by Online Reduction Using 2D-LC/MS Author Lucas Willmann Agilent Technologies, Inc. Abstract Recombinant monoclonal antibodies (mAbs) have become a popular class of biotherapeutics over recent years. To assess critical quality attributes in…