Applying cDDA-eXd on a timsOmni to the analysis of the Fab regions of antibodies originating from human serum

Top-down sequencing of bispecific antibodies by electron capture dissociation on a timsOmni platform Iuliia Stroganova1,2, Simon Ollivier1,2, Jean-François Greisch1,5, Athanasios Smyrnakis3, Mariangela Kosmopoulou3, Detlev Suckau4, Dimitris Papanastasiou3 and Albert J.R. Heck1,2 1Biomolecular Mass Spectrometry & Proteomics, NL, 2Netherlands Proteomics Center,…

Top‐Down Mapping of Protein Modifications by Multimodal MSⁿ on the New timsOmni Platform Francis Berthias1, Nurgül Bilgin2, Athanasios Smyrnakis3, Mariangela Kosmopoulou3, Christian Albers4, Jasmin Mecinović2, Dimitris Papanastasiou3, and Ole N. Jensen1 1 Department of Biochemistry and Molecular Biology; 2 Department…

AS MS 2 0 2 5 – M P 4 7 1 Advances in hardware design and function of the new timsOmni MS platform D. Papanastasiou1; A. Smyrnakis1; M. Kosmopoulou1; A. Grigoriadis1; I. Orfanopoulos1; N. Manolis1; I. Panagiotopoulos1; R. Gioves1;…

Application Note Biopharma Top-Down Sequence Analysis of Intact Proteins Using an Agilent AdvanceBio 6545XT LC/Q-TOF with ExD Authors Rachel Franklin and Mike Hare Agilent Technologies, Inc. Abstract Detailed proteoform characterization is crucial for understanding disease mode of action (MOA) and…