Top-down sequencing of bispecific antibodies by electron capture dissociation on a timsOmni platform

Applying cDDA-eXd on a timsOmni to the analysis of the Fab regions of antibodies originating from human serum Simon Ollivier1,2, Manuel Bauer5, Dina Schuster1,2, Athanasios Smyrnakis3, Jan Fiala1,2, Stuart Pengelley4, Oliver Raether4, Mariangela Kosmopoulou3, Detlev Suckau4, Jean-François Greisch1,5, Dimitris Papanastasiou3…

AS MS 2 0 2 5 – M P 4 7 1 Advances in hardware design and function of the new timsOmni MS platform D. Papanastasiou1; A. Smyrnakis1; M. Kosmopoulou1; A. Grigoriadis1; I. Orfanopoulos1; N. Manolis1; I. Panagiotopoulos1; R. Gioves1;…

AS MS 2 0 2 5 – M P 7 1 3 n Offline tandem MS workflows on the timsOmni platform for deep sequencing of intact proteins and mAbs Athanasios Smyrnakis1, Mariangela Kosmopoulou1, Anastasios Grigoriadis1, Florian Busch2, Stuart Pengelley3, Eduardo…

Analysis of bispecific and multispecific antibody therapeutics using native mass spectrometry on a modified hybrid Orbitrap mass spectrometer Corentin Beaumal1; Lisa Füssl1; Sara Carillo1; Kai Scheffler2; Cong Wang3; Heiner Koch3; Kelly Broster4; Jonathan Bones1,5 1NIBRT, Dublin, Ireland; 2Thermo Fisher Scientific,…