High-throughput Ion-Pairing Free Reversed Phase Analysis of Oligonucleotides using RapidFire Quadrupole Time-of-Flight Mass Spectrometry

Poster Reprint ASMS 2024 Poster number WP 573 Systematic Evaluation of Hydrophilic Interaction Liquid Chromatography Stationary Phases for Oligonucleotide Characterization by LC/MS Jordy J. Hsiao1, Alex Apffel1, Lee Bertram1, Andrea Angelo P. Tripodi2, Andrew Coffey2, Ta-Chen Wei3, Connor Flannery1 1Agilent…

Application Note Oligonucleotide Analysis Molecular Confirmation of Oligonucleotides Using Agilent LC/MSD XT and OpenLab CDS Author Abstract Lee Bertram and Brian Rivera Agilent Technologies, Inc. Mass spectrometry (MS) is an important tool for quality control during the development of synthetic…

Application Note High-throughput, Ion-Pairing-Free, HILIC Analysis of Oligonucleotides Using Agilent RapidFire Coupled to Quadrupole Time-of-Flight Mass Spectrometry Author Abstract Peter Rye, PhD Agilent Technologies, Inc. This application note describes a high-throughput, ion-pairing-free method for oligonucleotide characterization using the Agilent RapidFire…

Application Note BioPharma MS/MS Oligonucleotide Sequencing Using LC/Q-TOF with HILIC Chromatography Authors Guannan Li and Peter Rye Agilent Technologies, Inc. Introduction Oligonucleotides are commonly analyzed by liquid chromatography/mass spectrometry (LC/MS) in negative ion polarity mode using ion-pairing reversed‑phase (IP‑RP) methods.…