Systematic Evaluation of Hydrophilic Interaction Liquid Chromatography Stationary Phases for Oligonucleotide Characterization by LC/MS

Connor Flannery1, Jordy Hsiao1, Lee Bertram1, Andrea Angelo P. Tripodi2, Andrew Coffey2, Anne Blackwell 4, Ta-Chen Wei4, Chae-Young Ryu3 Emerging Alternative Analytical Techniques for Oligonucleotide Separation and Purification: HILIC & SEC Introduction 1Agilent Technologies, Santa Clara, CA 2Agilent Technologies, Church…

Poster Reprint HPLC 2024 Poster number 274 Emerging Alternative Analytical Techniques for Oligonucleotide Separation and Purification: HILIC & SEC 1 Connor Flannery1, Jordy Hsiao1, Lee Bertram1, Andrea Angelo P. Tripodi2, Andrew Coffey2, Anne Blackwell4, Ta-Chen Wei4, Chae-Young Ryu3 1Agilent Technologies,…

Application Note High-throughput, Ion-Pairing-Free, HILIC Analysis of Oligonucleotides Using Agilent RapidFire Coupled to Quadrupole Time-of-Flight Mass Spectrometry Author Abstract Peter Rye, PhD Agilent Technologies, Inc. This application note describes a high-throughput, ion-pairing-free method for oligonucleotide characterization using the Agilent RapidFire…

Application Note BioPharma MS/MS Oligonucleotide Sequencing Using LC/Q-TOF with HILIC Chromatography Authors Guannan Li and Peter Rye Agilent Technologies, Inc. Introduction Oligonucleotides are commonly analyzed by liquid chromatography/mass spectrometry (LC/MS) in negative ion polarity mode using ion-pairing reversed‑phase (IP‑RP) methods.…