Spectinomycin and Lincomycin on a Thermo Scientific™ Acclaim™ PolarAdvantage (PA) Column with Electrochemical Detection

APPLICATION NOTE 72880 Determination of spectinomycin and related impurities in spectinomycin dihydrochloride Authors Jingli Hu and Jeffrey Rohrer Goal Thermo Fisher Scientific Sunnyvale, CA To demonstrate that spectinomycin content can be determined and related impurities separated with a Thermo Scientific™…

Table of contents Summary Overview Excipient function Measurement Charged Aerosol Detector Universal detection Uniform response Working principles (U)HPLC Systems with CAD Columns choice Excipients overview Adjuvants Amino acids Carbohydrates Counterions Nonionic surfactants Polyethylene glycol Proteins and excipients HPLC-Charged Aerosol Detection…

Acclaim columns overview Columns for challenging separation needs Broad range of HPLC and UHPLC columns Phenyl-1 C30 Mixed-Mode WAX-1 Mixed-Mode WCX-1 Organic acid Surfactant Plus AmG C18 Explosives E2 M Mixed-Mode HILIC-1 ix ed -m Trinity P1 od e Carbamate…

Sialic Acid Determination in Glycoproteins: Comparison of Two Liquid Chromatography Methods Deanne Hurum and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA, USA Introduction Experimental Sialic acids are critical in determining glycoprotein bioavailability, function, stability, and eventual catabolism. Although over 50…