ICSialic Acid Determination in Glycoproteins: Comparison of Two Liquid Chromatography Methods

Application Update 180 Direct Determination of Sialic Acids in Glycoprotein Hydrolyzates by HPAE-PAD INTRODUCTION Sialic acids are critical in determining glycoprotein bioavailability, function, stability, and metabolism.1 Although over 50 natural sialic acids have been identified,2 two forms are commonly determined…

Application Update: 181 Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD Deanna Hurum and Jeff Rohrer Introduction • Neuraminic Acids • Anion Exchange • Pulsed Amperometric Detection • CarboPac PA20 Fast Sialic Acid Column • High-Throughput Analysis Glycoprotein sialylation…

APPLICATION NOTE 74121 Determination of glycoprotein sialic acid composition using HPAE-PAD in dual eluent generation cartridge mode Authors: Jingli Hu and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Keywords: Dionex CarboPac PA20-1 mm column, Dionex ICS-6000 HPIC system, electrochemical detection,…

Table of Contents Introduction Applications Innovative Analytical Technologies Monosaccharide and Sialic Acid Determinations in Biosimilars Using HPAE-PAD Introduction Table of Contents Introduction Applications Innovative Analytical Technologies A large number of innovator biotherapeutics are currently losing patent protection, resulting…