Analysis of Oligonucleotides Using Ion-Pairing Alternatives on the Agilent Pro iQ Plus

Application Note Biopharmaceuticals Analysis of Oligonucleotides Using an Ion-Pairing-Free Reversed-Phase Method with TOF LC/MS Authors Lee Bertram and Jordy Hsiao Agilent Technologies, Inc. Abstract This application note details a reversed-phase separation methodology for oligonucleotides without the use ion pairing reagents.…

Table of contents Fast Track to Certainty: Confident Biopharma Decisions with LC-Single Quadrupole Mass Detection How single quadrupole LC/MS simplifies identification, purification, and stability assessment in biopharma Table of Contents Introduction��������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������������� 3 Consolidating Biopharma QC Workflows: LC/UV Versus Single Quadrupole…

Poster Reprint ASMS 2025 Poster number MP 230 Small-Footprint Capillary UHPLC/MS Technology Significantly Reducing Consumption of PFAS Containing Modifiers for Fast and High-Resolution Separation of Synthetic Oligonucleotides Xiaoli Dong1, Cary Simpson2, Sam Foster2, Greg Ward2, Patrick Batoon1 1Agilent Technologies Inc,…

Poster Reprint ASMS 2025 Poster number ThP 565 High-throughput Ion-Pairing Free Reversed Phase Analysis of Oligonucleotides using RapidFire Quadrupole Time-of-Flight Mass Spectrometry Guannan Li and Lee Bertram Agilent Technologies, Inc., Santa Clara, CA Introduction Experimental Oligonucleotides are commonly analyzed by…