Separation of Pyrimidine and Purine Bases in HILIC mode with AZURA Compact

Polar Analytes: C18 Didn’t Work, Now What? Mark Powell Agilent Technologies Technical Support Page 1 C18 Doesn’t Always Work… Page 2 Pore Dewetting or Phase Collapse •Alkyl phases such as C8 or C18 can exhibit poor retention or reproducibility of…

[ APPLICATION NOTE ] Targeted Quantification of Cell Culture Media Components by LC-MS WANG Xiaoxia, LIU Yi, WANG Hui, JIA Zhengwei Waters Technology (Shanghai) Co., Ltd. APPLICATION BENEFITS ■ ■ A fast and universal LC-MS/MS method Cell culture medium components…

Separation of Hydroxybenzoic acids with AZURA Compact Method HILIC Mode HPLC VPH0060J Column: Eurospher II 100-5 HILIC, 150 x 3 mm ID Phase: Eurospher II 100-5 HILIC Conditions: Eluent: Gradient: Flow rate: Temperature: Volume: Order No. 15CE120E2J A: 5 mM…

Thinking Outside the C18 Box 1 Modes of HPLC • Reversed-phase • C18, C8, other non-polar phases • Phenyl, PFP, other more polar phases • Ion Pairing • HILIC • Silica • Amide • Zwitterionic • Hydroxyl-based • Chiral •…