Antibody subunit analysis via inline electrochemical reduction of an intact antibody using the ROXY potentiostat coupled to a Vanquish UHPLC – Q Exactive Plus MS system

Application note | 001391 Biopharma Improved and universal inline electrochemical reduction and subunits LC-MS analysis of multiple classes of monoclonal antibodies Authors Application benefits Sara Carillo, Tomos E. Morgan, Hendrik1 1 • Reduced mAb and reduced subunits analysis can be…

ROXY™ Exceed Electrochemistry System for EC-MS Proteomics Metabolism Drug Stability Environmental Synthesis Electrochemistry – Mass Spectrometry (EC–MS) Why? Over 90% of the worlds existing compounds possess REDOX properties, making them either oxidizable or reducible. By injecting your analyte into an…

APPLICATION NOTE 72854 Antibody subunit analysis workflow on a quadrupole-Orbitrap mass spectrometer: from optimized sample preparation to data analysis Authors Kai Scheffler, Thermo Fisher Scientific, Germering Eugen Damoc, Thermo Fisher Scientific, Bremen Keywords Antibody subunits, Orbitrap, Q Exactive BioPharma, sample…

HPLC Columns LC-MS analysis of intact and subunit-level mAb enabled by a novel monodisperse supermacroporous reversed-phase platform Audrius Žvirblis1, Odeta Dembovskytė1, Ke Ma2, Christof Mitterer3, Mauro De Pra4 and Frank Steiner5 1Thermo Fisher Scientific, Vilnius, Lithuania, 2Thermo Fisher Scientific, CA,…