Evaluating IonHance Hexafluoroisopropanol (HFIP) for Enhanced LC-MS Oligonucleotide Analysis

Application Note Enhanced Resolution for Longer Oligonucleotide Analytes With a MaxPeak™ Premier Oligonucleotide BEH C18 300 Å Column Balasubrahmanyam Addepalli, Makda Araya, Matthew A. Lauber Waters Corporation Abstract Ion-pairing reversed phase liquid chromatography is widely used to characterize the identity,…

Application Note Platform Ion Pairing RPLC Method for Oligonucleotides Using High Throughput 20 mm Length Columns Szabolcs Fekete, Mateusz Imiolek, Matthew A. Lauber Waters Corporation Abstract A novel generic (platform) method is proposed for the separation of oligonucleotide size variants…

Poster Reprint ASMS 2026 MP 528 Improved Oligonucleotide LC–MS Performance through Inert Column Hardware and Alternative Separation Strategies Andrew Coffey1; Daniel Meston1; Chae-Young Ryu2; Anne Blackwell2; Jordy Hsiao4 1Agilent Technlogies, Church Stretton, United Kingdom; 2 Agilent Technologies, Seoul, South Korea;…

Application Note BioPharma/Pharma Eliminate Metal Ions from Your Oligonucleotide LC/MS Analysis Avoid undesirable metal interactions with Agilent Ultra Inert HPLC column hardware and fully biocompatible LC instrumentation Authors Abstract Andrew Coffey, Andrea Angelo P. Tripodi, Anne Blackwell, and Jordy Hsiao…