Intact Analysis of Biopharmaceuticals by Hydrophobic Interaction/Reversed Phase 2D-LC/MS System

Sandeep kondaveetil1, Andy Coffey2, Priya Jayaraman1, Linda Llyod2, Suma Ramagiri1 Hydrophobic Interaction Chromatography- Native Mode Separation of Bio-Therapeutic Workflows 1. Agilent Technologies, Inc., Wilmington, DE USA 2. Agilent Technologies, Inc., Church Stretton, UK Experimental LC Conditions – mAb oxidized variant…

Analysis of Monoclonal Antibodies Using Multiple Heart-cutting Hydrophobic Interaction/Reversed Phase 2D-LC/MS Application Note Biotherapeutics & Biologics Author Abstract Gregory Staples Hydrophobic interaction chromatography (HIC) is a popular approach for both Agilent Technologies, Inc. downstream processing and analytical scale analysis of…

Agilent-NISTmAb Intact Analysis Using Hydrophobic Interaction Agilent BioHPLC Columns Application Compendium Contents Agilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted from NISTmAb RM 8671 batch. Quality control (QC) testing is performed using Agilent LC-MS system. QC batch release test includes aggregate…

Application Note Biopharma/Pharma Characterization of Antibody‑Drug Conjugates Using 2D-LC and Native MS Authors David L. Wong and Sarah M. Stow Agilent Technologies, Inc. Abstract Antibody drug conjugates (ADCs), which comprise a monoclonal antibody (mAb) conjugated to a small molecule drug…