Top-Down CDR Sequencing of Native Intact mAbs Through Deconvolution of HC+LC Mixture Spectra

Poster Reprint ASMS 2023 Poster number TP 024 Top-down characterization of native monoclonal antibodies obtained with electron capture dissociation on Q-TOF instruments Yury V. Vasil’ev1, Rachel Franklin1, Michael C. Hare1, Adrian Guthals1, Joseph S. Beckman2 1e-MSion 97332 2Oregon – A…

Transformative Workflows for Protein Characterization: ECD and Multidimensional Separations Expert Insights Sponsored by Expert Insights Contents Introduction 3 The Role of Electron Capture Dissociation in Biomolecular Analysis Adapted from Cooper, et al. 4 Offline Coupling of Hydrophobic Interaction Chromatography– Capillary…

Poster Reprint ASMS 2024 Poster number WP 470 High-Efficiency Electron Capture Dissociation of Peptides and Proteins After Collision-Induced Unfolding and Ion Mobility Joseph C. Meeuwsen1, Yury V. Vasilev1, Ruwan T. Kurulugama1, Valery G. Voinov1 1Agilent Technologies, Inc., Santa Clara, CA…

Application Note Biopharma Identification of Amino Acid Isomers Using Electron Capture Dissociation in the Agilent 6545XT AdvanceBio LC/Q-TOF System Authors Rachel Franklin and Joseph Meeuwsen Agilent Technologies, Inc. Abstract Accurately determining the amino acid sequence is crucial for understanding a…