Monitoring oxidation in recombinant monoclonal antibodies at subunit level through two-dimensional liquid chromatography coupled with mass spectrometry

There is a growing trend to obtain intact mass information as well as the glycan profile in the QC of mAbs using high resolution mass spectrometers. The most commonly employed LC/MS method is to desalt mAbs via reverse phase liquid…

Characterize Fab and Fc Fragments by Cation-Exchange Chromatography Application Note Biologics and Biosimilars Authors Abstract Isabel Vandenheede, Emmie Dumont, This application note describes the use of the Agilent Bio MAb weak Pat Sandra, and Koen Sandra cation-exchange column in measuring…

Agilent BioHPLC Columns Characterization of NIST Monoclonal Antibody Critical Quality Attributes Application Compendium Contents Agilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted from NISTmAb RM 8671 batch. Quality control (QC) testing is performed using Agilent LC-MS system. QC batch release test…

Poster Note 643 98 Fast Analysis of Therapeutic Monoclonal Antibodies, Fragments, and Oxidation Variants Using a Super-Macro Porous Reversed-Phase Coupled with anFragments, FastAnalysis Analysis of Therapeutic TherapeuticColumn Monoclonal Antibodies, Fast of Monoclonal Antibodies, Fragments, a Orbitrap Mass Spectrometer Column Coupled…