PEGS: HIGH THROUGHPUT CYSTEINYLATION SCREENING AT MAB SUBUNIT LEVEL USING LC-MS MONITORING WORKFLOW

HIGH THROUGHPUT CYSTEINYLATION SCREENING AT MAB SUBUNIT LEVEL USING LC-MS SCREENING WORKFLOW Samantha Ippoliti1, Bradley Prater2, Ying Qing Yu1, Magnus Wetterhall3 1Waters Corporation, Milford, MA; 2Similis Bio, Sunnyvale, CA, USA; 3Waters Corporation, Solna, Sweden INTRODUCTION RESULTS & DISCUSSION Monoclonal antibodies…

Application Note Efficient Non-Reduced mAb Subunit LC-MS Analysis to Screen for Modifications of Unpaired Cysteines in Innovator and Biosimilars Samantha Ippoliti, Bradley Prater, Ying Qing Yu, Magnus Wetterhall Similis Bio, Waters Corporation Abstract This application note highlights a collaboration between…

Application Note Similis Bio - Streamlined mAb Subunit LCMS Workflow for Multiple Attribute Monitoring of Biosimilar mAb Candidates During Bioprocessing and Development Samantha Ippoliti, Jared Young, Katy McNally, Caitlin Hanna, Ying Qing Yu, Bradley Prater, Mark D. Wrona, Emma Harry…

Application Note Biopharma/Pharma Hybrid UV-Vis/MS Assay for Free Cysteine Determination in Monoclonal Antibodies Using the Agilent Cary 3500 UV-Vis and Agilent 6545XT AdvanceBio LC/Q-TOF MS Author Abstract Ravindra Gudihal Agilent Technologies, Inc. Cysteine residues in monoclonal antibodies (mAbs) play key…