HIGH THROUGHPUT CYSTEINYLATION SCREENING AT MAB SUBUNIT LEVEL USING LC-MS SCREENING WORKFLOW

Application Note Efficient Non-Reduced mAb Subunit LC-MS Analysis to Screen for Modifications of Unpaired Cysteines in Innovator and Biosimilars Samantha Ippoliti, Bradley Prater, Ying Qing Yu, Magnus Wetterhall Similis Bio, Waters Corporation Abstract This application note highlights a collaboration between…

HIGH THROUGHPUT CYSTEINYLATION SCREENING AT MAB SUBUNIT LEVEL USING LC-MS MONITORING WORKFLOW Samantha Ippoliti1, Bradley Prater2, Ying Qing Yu1, Magnus Wetterhall3, Guillaume Bechade4 1 Waters Corporation, Milford, MA USA; 2Similis Bio, Sunnyvale, CA; 3Waters Corporation, Solna, Sweden; 4Waters Corporation, Wilmslow,…

Application Note Biopharma/Pharma Hybrid UV-Vis/MS Assay for Free Cysteine Determination in Monoclonal Antibodies Using the Agilent Cary 3500 UV-Vis and Agilent 6545XT AdvanceBio LC/Q-TOF MS Author Abstract Ravindra Gudihal Agilent Technologies, Inc. Cysteine residues in monoclonal antibodies (mAbs) play key…

Application Note Expanding the Antibody-Oligo Conjugate (AOC) Characterization Toolbox: Part 3Partial and Complete AOC Conjugation Site Determination Samantha Ippoliti, Ying Qing Yu Waters Corporation, United States Note: This is the third application note in a three-part series on AOC characterization.…