Complete Characterization of a Cysteine- linked Antibody-Drug Conjugate Performed on a Hybrid Quadrupole-Orbitrap Mass Spectrometer with High Mass Range

High Resolution Mass Spectrometry of Antibody Drug Conjugates Using the Orbitrap Mass Analyzer Kai Scheffler1, Eugen Damoc2, Aaron Bailey3, and Jonathan Josephs3 Thermo Fisher Scientific, 1Dreieich, Germany, 2Bremen, Germany, 3San Jose, USA Figure 1: Operating modes for the three major…

Integrated characterization of a lysine-linked antibody-drug conjugate by native intact mass analysis and peptide mapping performed on a hybrid quadrupole-Orbitrap mass spectrometer with high mass range Aaron O. Bailey 1, Stephane Houel 1, Eugen Damoc 2, Kai Scheffler 3, Jonathan…

APPLICATION NOTE 72511 Complete characterization of a lysine-linked antibody drug conjugate by native LC/MS intact mass analysis and peptide mapping Authors Aaron O. Bailey,1 Stephane Houel,1 Kai Scheffler,2 Eugen Damoc,3 Jennifer Sutton,1 Jonathan L. Josephs1 Thermo Fisher Scientific 1 San…

Agilent BioHPLC columns Characterization of Antibody-Drug Conjugate Critical Quality Attributes Application compendium Contents Introduction3 Featured application notes 2 PLRP-S Polymeric Reversed-Phase Column for LC/MS Separation of mAbs and ADC (5991-7163EN) 5 Intact- and Peptide-Level Characterization of Trastuzumab Emtansine In Vitro…