Characterization of adeno-associated viral proteins proteoforms using a top-down MS approach on a UHPLC-Orbitrap Ascend Tribrid mass spectrometer

Characterization of Adeno-Associated Viral assemblies on an Ultra-High Mass Range Hybrid QuadrupoleOrbitrap Mass Spectrometer Kristina Srzentić1, Eugen Damoc2, Marc Günder1 1Thermo Fisher Scientific, Reinach, Switzerland 2Thermo Fisher Scientific, Bremen, Germany ABSTRACT Purpose: Evaluate the performance of the Thermo Scientific™ Q…

APPLICATION NOTE 000253 Highly sensitive intact mass analysis of AAV capsid proteins using a UHPLC-FLDHRAM MS platform Authors: Reiko Kiyonami¹, Kristina Srzentić², Rosa Viner¹, Weijing Liu¹, Min Du², Katy Irvin³, Kenneth Thompson³, Chao Yan Liu³, Jon Zmuda³ ¹ Thermo Fisher…

Multiple ion activations and proton transfer charge reduction facilitate the characterization of capsid proteoforms of recombinant adeno-associated virus 6 (rAAV6) Jake T. Kline1, Kristina Srzentić2, Katalyn Irvin3, Christopher Mullen4, Romain Huguet4, John EP Syka4, Luca Fornelli1 1University of Oklahoma, Norman,…

Application note | 001120 Biopharma Assessing key attributes of adeno-associated viral proteins using HPLC-FLD-intact mass analysis Authors Application benefits Reiko Kiyonami1, Roberto Gamez1, Min • Rapid ratio assessment of adeno-associated virus (AAV) structural proteins VP1:VP2:VP3 through sensitive fluorescence detection •…