ENHANCED TOP-DOWN MASS SPECTROMETRY PERFORMANCE WITH A HYBRID QUADRUPOLE-MULTIREFLECTING TIME-OF-FLIGHT SYSTEM

Next-generation MALDI top-down sequencing of protein biotherapeutics – expanding the scope of timsTOF technology Next-generation MALDI top-down sequencing (MALDI-TDS) improves top-down sequence analysis of biopharmaceutical proteins, as demonstrated in this note for adalimumab subunits, recombinant SARS-CoV-2 S-glycoprotein RBD expression products,…

Application Note Biopharma Top-Down Sequence Analysis of Intact Proteins Using an Agilent AdvanceBio 6545XT LC/Q-TOF with ExD Authors Rachel Franklin and Mike Hare Agilent Technologies, Inc. Abstract Detailed proteoform characterization is crucial for understanding disease mode of action (MOA) and…

Methods Sample Preparation and Liquid Chromatography Protein standards (Ubiquitin, Carbonic anhydrase and Enolase) were purchased from Sigma Aldrich. Proteins were infused at 1pmol/ul at a flow rate 5ul/min for direct infusion experiments. Recombinant Human TROP2 protein (extracellular domain) was purchased…

Poster Reprint ASMS 2025 Poster number TP 703 In-Source Charge Reduction Enhances Top-Down Characterization of Denatured Proteins Rachel Franklin1; Yury V. Vasilev1; John Mehl2 1Agilent Technologies, Inc., Corvallis, OR 2Agilent Technologies, Inc., Wilmington, DE Introduction Experimental Denatured proteins often yield…