Rapid analysis of titer, aggregate and intact mass of antibody therapeutics using multi-dimensional liquid chromatography coupled with native mass spectroscopy

Application note | 003310 Biopharma Monitoring of multiple quality attributes of intact monoclonal antibodies from bioreactors by switchable 2D-LC-MS Authors Application benefits Maria Grübner , Kai Scheffler , 1 1 • Heart-cut 2D-LC with protein A affinity chromatography (ProA) of…

Poster Reprint ASMS 2023 Poster number MP 626 Multi-attribute monitoring of aggregates and charge variants of monoclonal antibody through native 2DSEC-MS-WCX-MS Sunil Kumar1, Tushar Sharad Savane1, Vadiraja Bhat2, Mike Knierman3, Anurag Singh Rathore1 1 Department of Chemical Engineering, Indian Institute…

High Efficiency Protein A Affinity Columns for the LC-MS and 2D LC-MS Analysis of mAb and msAb A quiver of LC-MS based approaches for the sub-unit and intact and analysis of intact monoclonal antibodies (mAb) and multispecific antibodies (msAb) directly…

Poster Reprint ASMS 2023 Poster number MP 625 A native multi-dimensional monitoring workflow for at-line characterization of mAb titer, size, charge, and glycoform heterogeneities in cell culture supernatant Sanghati Bhattacharya1, Shashank Jain2 , Vadiraja Bhat3, Srishti Joshi1, Anurag S. Rathore1…