Mastering HILIC-Z Separation for Polar Analytes

Application Note Small Molecule Pharma Retaining and Separating Polar Molecules—A Detailed Investigation of When to Use HILIC Versus a Reversed-Phase LC Column Author Anne Mack Agilent Technologies, Inc. Abstract This work examines when to use hydrophilic interaction LC (HILIC) and…

Why Use HILIC and HIC Chromatography? Theory and method development strategies Mohit Patel, PhD LC Applications Engineer October 8, 2024 1 10/08/2024 DE-001530 AGILENT CONFIDENTIAL Agenda 1 What is HILIC and when should you consider it? 2 How to approach…

Technical Overview Hydrophilic Interaction Chromatography Method Development and Troubleshooting Introduction Hydrophilic interaction chromatography (HILIC) is a rapidly growing technique within the field of high-performance liquid chromatography (HPLC). Even the most polar compounds can be separated with HILIC using the same…

HILIC Chromatography: When and How? Golnar Javadi Applications Engineer LC Columns and Consumables Technical Support January 25, 2022 Agenda 1 What is HILIC and when should you consider it? 2 HILIC method development • • 3 Tips and tricks for…