Glycoprotein Oligosaccharide Analysis Using High- Performance Anion-Exchange Chromatography

Application Note 105 Glycosylation Analysis of Human Serum Transferrin Glycoforms Using Pellicular Anion-Exchange Chromatography INTRODUCTION Glycoforms and Protein Sialylation Glycoproteins are proteins with a carbohydrate attached to the polypeptide backbones through one or more glycosylation sites. Oligosaccharides can be linked…

APPLICATION UPDATE 176 Preparation of peptide N-Glycosidase F digests for HPAE-PAD analysis Authors Introduction Pranathi Perati and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Glycosylation is one of the most important post-translational modifications in eukaryotic cell proteins. Glycoproteins are involved…

Application Note 215 Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG Using the CarboPac PA200 Column Introduction High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) is a widely used technique for determining an extensive set of carbohydrates including, but…

HPAE-PAD Analysis of N-linked Oligosaccharides from Glycoproteins Using Dual Eluent Generation Cartridge Mode Beibei Huang, Lillian Chen, Joachim Weiss, and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA , USA, 94085 ABSTRACT Data Analysis Purpose: To demonstrate Dual Eluent Generation Cartridge…